This invention relates to an optimized and improved
erythrose-4-
phosphate dehydrogenase (Epd)
mutant and its encoding
gene. Based on SEQ ID NO.2, at least one
amino acid is replaced at positions 13, 14, 16, 17, 31, 38, 41, 63, 67, 78, 79, 80, 83, 86, 95, 99, 109, 125, 134, 142, 143, 153, 156, 177, 179, 184, 214, 216, 218, 225, 234, 239, 243, 244, 247, 248, 281, and 318. Compared with wild-type Epd, the
mutant of this invention can increase the
relative yield of
vitamin B6 produced by
fermentation to varying degrees (4%–212%), thus showing good application prospects in the field of microbial
fermentation for
vitamin B6 production.