Phosphatidylserine taurine direct drinking powder for improving memory and Alzheimer's disease and preparation method thereof

Abstract

The invention provides phosphatidylserine and taurine direct drinking powder for improving memory and Alzheimer's disease and a preparation method, and relates to the field of direct drinking powder. The phosphatidylserine taurine direct drinking powder for improving memory and Alzheimer's disease is prepared from the following raw materials in percentage by mass: 30-40% of erythritol, 10-20% of taurine, 10-20% of maltodextrin, 10-20% of 28%-38% of resistant dextrin; 12% to 15% of phosphatidylserine; 5%-10% of theanine in tea leaves; 2%-8% of rhizoma polygonati powder; 2%-6% of menthol; 0%-1% of citric acid; 0%-1% of taurine; 10%-1% of vitamin B; and 20%-1% of vitamin B. Core active components are phosphatidylserine and taurine, a compound of citric acid and menthol can promote absorption of phosphatidylserine and taurine, resistant dextrin and polygonatum polysaccharide form an eutectic network as a carrier, phosphatidylserine and taurine are subjected to spray drying under the condition that the pH value is 4.5 to form an ionic bonding body, and the antibacterial activity of the product is improved. The direct drinking powder can rapidly dissolve out and release active ingredients through the acid-base property of oral mucosa, and the dosage form is rapidly released through the oral mucosa, so that the bioavailability is greatly improved.

Description

Technical Field

[0001] This invention relates to the field of direct drinking powder technology, specifically to a phosphatidylserine taurine direct drinking powder for improving memory and Alzheimer's disease, and its preparation method. Background Technology

[0002] There are approximately 55 million Alzheimer's patients worldwide. Existing drugs mostly target Aβ protein, and their efficacy against abnormal phosphorylation of Tau protein is limited. Phosphatidylserine (PS), as a key component of cell membranes, can inhibit excessive phosphorylation of Tau, but it is subject to absorption disorders.

[0003] Most existing phosphatidylserine formulations use PS+DHA soft capsules, which promote absorption through fatty acids and encapsulate the phosphatidylserine with lecithin during processing to improve stability. This approach means that phosphatidylserine mainly relies on gastrointestinal absorption, resulting in a first-pass effect loss of over 40%. At the same time, DHA competes with phosphatidylserine (PS) for the transporter protein (MFSD2A), leading to low utilization and a peak time of up to 3.5 hours. Existing phosphatidylserine formulation designs do not avoid the first-pass effect in the liver and do not solve the problem of brain-targeted delivery of phosphatidylserine. The complex formulations also lead to mutual inhibition of active ingredients. Summary of the Invention

[0004] To address the shortcomings of existing technologies, this invention provides a phosphatidylserine taurine direct-drinking powder and its preparation method for improving memory and Alzheimer's disease. It solves the problems of existing phosphatidylserine dosage form designs failing to avoid the first-pass effect in the liver, failing to address the brain-targeted delivery problem of phosphatidylserine, and the mutual inhibition of active ingredients caused by compound formulations.

[0005] To achieve the above objectives, the present invention provides the following technical solution: a phosphatidylserine taurine direct-drinking powder for improving memory and Alzheimer's disease, composed of the following raw materials in the indicated mass percentages:

[0006] Erythritol 30%–40%;

[0007] Resistant dextrin 28%–38%;

[0008] Phosphatidylserine 12%-15%;

[0009] Tea contains 5%–10% theanine;

[0010] Polygonatum powder 2%–8%;

[0011] Menthol 2%–6%;

[0012] Citric acid 0%–1%;

[0013] Taurine 0%–1%;

[0014] Vitamin B1 0%–1%;

[0015] Vitamin B2 0%–1%.

[0016] Preferably, a phosphatidylserine taurine direct-drinking powder for improving memory and Alzheimer's disease is composed of the following raw materials in weight percentages:

[0017] 35% erythritol;

[0018] 32% resistant dextrin;

[0019] Phosphatidylserine 15%;

[0020] Tea contains 7% theanine;

[0021] Polygonatum powder 6%;

[0022] Menthol 4%;

[0023] Citric acid 0.96%;

[0024] Taurine 0.03%;

[0025] Vitamin B1 0.005%;

[0026] Vitamin B2 0.005%.

[0027] A preferred method for preparing a phosphatidylserine taurine direct-drinking powder for improving memory and Alzheimer's disease includes the following steps:

[0028] Step 1: Boil and dry the resistant dextrin at 52℃-55℃ for 1h-1.5h, pass it through a 60-mesh sieve, and then divide it into small and large portions of 0.2 and 0.8 by mass. Mix erythritol, phosphatidylserine, tea theanine and polygonatum powder and pass it through a 60-mesh sieve.

[0029] Step 2: Boil and dry citric acid, menthol and taurine at 52℃-55℃ for 1h-1.5h, then pass them through a 60-mesh sieve. Also pass vitamin B1 and vitamin B2 through a 60-mesh sieve.

[0030] Step 3: Mix the sifted erythritol, phosphatidylserine, tea theanine, polygonatum powder and a small amount of resistant dextrin from Step 1, and mix them in a mixer for 10 minutes to obtain Mixed Powder 1. Mix the sifted citric acid, menthol, taurine, vitamin B1 and vitamin B2 from Step 2 in a mixer for 20 minutes to obtain Mixed Powder 2.

[0031] Step 4: Place Mixed Powder 1 and Mixed Powder 2 in a mixer and mix for 20 minutes to obtain phosphatidylserine taurine direct drinking powder that improves memory and Alzheimer's disease. Pack the direct drinking powder into bags with a bag size of 1.0g / bag. After bagging, inspect and store the powder in the warehouse for sale.

[0032] Preferred application of a phosphatidylserine taurine direct-drinking powder for improving memory and Alzheimer's disease, used to enhance memory and Alzheimer's patients.

[0033] This invention provides a phosphatidylserine taurine direct-drinking powder for improving memory and Alzheimer's disease, and its preparation method. It has the following beneficial effects:

[0034] The core active ingredient of this invention is phosphatidylserine plus taurine. The complex of citric acid and menthol can promote the absorption of phosphatidylserine and taurine. Resistant dextrin and polygonatum polysaccharide form a co-crystal network as a carrier. Phosphatidylserine and taurine are spray-dried under pH 4.5 conditions to form an ionic bond, enabling the direct drinking powder to construct a co-transport system of taurine and phosphatidylserine. Taurine mediates the crossing of the blood-brain barrier by TAUT transporters, which greatly increases the blood-brain barrier permeability. Citric acid and menthol synergistically open the oral mucosa and tightly connect it, which increases the permeability coefficient of phosphatidylserine by 8.3 times. The direct drinking powder can quickly dissolve and release the active ingredient through the acidity and alkalinity of the oral mucosa. The rapid release dosage form through the oral mucosa makes the bioavailability much higher than that of similar products. Attached Figure Description

[0035] Figure 1 A simplified process diagram of the preparation method of the phosphatidylserine taurine direct drinking powder of the present invention;

[0036] Figure 2 This is a trajectory diagram of the mine field results from the mine field experiment of the present invention;

[0037] Figure 3 A statistical chart of the time to enter the central zone in the mine field experiment of this invention;

[0038] Figure 4 A bar chart showing the time of entry into the central zone in the mine field experiment of this invention.

[0039] Figure 5 The Y-maze results of the Y-maze experiment of the present invention are analyzed as shown in the figure;

[0040] Figure 6 This is a bar chart showing the Y-maze rotation ratio in the Y-maze experiment of this invention. Detailed Implementation

[0041] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0042] Example 1:

[0043] This invention provides a phosphatidylserine taurine direct-drinking powder for improving memory and Alzheimer's disease, composed of the following raw materials by weight percentage:

[0044] 35% erythritol;

[0045] 32% resistant dextrin;

[0046] Phosphatidylserine 15%;

[0047] Tea contains 7% theanine;

[0048] Polygonatum powder 6%;

[0049] Menthol 4%;

[0050] Citric acid 0.96%;

[0051] Taurine 0.03%;

[0052] Vitamin B1 0.005%;

[0053] Vitamin B2 0.005%;

[0054] In actual production, the above direct drinking powder formula can be weighed directly according to the percentage ratio. Based on actual production, the core active ingredients are phosphatidylserine and taurine. Citric acid and menthol complex can promote the absorption of phosphatidylserine and taurine. Resistant dextrin and polygonatum polysaccharide form a co-crystal network as a carrier. Phosphatidylserine and taurine form ionic bonds under pH 4.5 conditions through spray drying, enabling the direct drinking powder to construct a co-transport system of taurine and phosphatidylserine. Taurine mediates the crossing of the blood-brain barrier of phosphatidylserine through the TAUT transporter, increasing the blood-brain barrier permeability by at least 58%. Citric acid and menthol synergistically open the oral mucosa and tightly connect it, increasing the permeability coefficient of phosphatidylserine by 8.3 times. The direct drinking powder can quickly dissolve and release the active ingredients through the acidity and alkalinity of the oral mucosa. The rapid release dosage form through the oral mucosa increases the bioavailability to 82%.

[0055] The following experiment verifies that this direct-drinking powder can improve memory and alleviate Alzheimer's disease:

[0056] a. Experimental principle:

[0057] Diphosphatidylserine can enhance synaptic plasticity and significantly improve the learning and memory abilities of AD model mice by promoting the synthesis and release of neurotransmitters such as acetylcholine. It can also reduce Aβ deposition and hyperphosphorylated Tau protein levels in the brain, thus decreasing the formation of neurofibrillary tangles. Combined use with unsaturated fatty acids such as DHA can enhance its protective effect on neuronal activity.

[0058] Taurine has neuroprotective effects by regulating brain metabolite levels, improving cerebral hemodynamics, and protecting neurons from oxidative stress damage. It can also alleviate mood disorders by significantly reducing anxiety symptoms and improving depressive-like behaviors in AD model mice. Taurine can also target AD pathology by inhibiting the synergistic toxicity of Aβ and tau proteins, reducing abnormal microglial activation.

[0059] This experiment tested whether the sample could alleviate Alzheimer's disease by administering it to three-wheeled rats.

[0060] b. Experimental instruments and materials:

[0061] Instruments / Materials Model / Batch Number factory SPF-grade three-turn mouse 24 weeks SPF-grade animal facility of Shandong First Medical University Mining Behavioral Equipment Mouse-specific Shandong First Medical University Y-maze equipment Mouse-specific Shandong First Medical University

[0062] c. Experimental methods:

[0063] SPF-grade triple-transfer mice were randomly divided into two groups of three each: a control group and a sample treatment group. The control group was fed pure water, while the sample treatment group was fed the test sample at a concentration of 15 g / L. After two weeks of feeding, behavioral tests in the mine and Y-maze were performed.

[0064] Mine Field Experiment:

[0065] Three days prior to the experiment, mice were transferred to the behavioral laboratory (with the same photoperiod). They were kept still for one hour before the experiment to avoid interference. Environmental control: Light: 50-100 lux (central area). Noise: <50 dB. Temperature: 22±1°C. Apparatus cleaning: Wipe with ethanol after each test to avoid odor residue. Gently place the mice in the central area of ​​the open field and immediately start recording. Recording time: 10 minutes. After the test, gently remove the mice and return them to their cages. Clean the apparatus and wait 5 minutes before testing the next mouse (to avoid odor interference). Randomize the mice by cage number to avoid time sequence bias. Cross-test mice within the same group (e.g., alternating between control and experimental groups).

[0066] Y-maze experiment:

[0067] Three days prior to the experiment, mice were transferred to the behavioral laboratory (with the same photoperiod). They were kept still for one hour before the experiment to avoid interference. Environmental control: Light: 50-100 lux (central area). Noise: <50 dB. Temperature: 22±1°C. Apparatus cleaning: Wipe with ethanol after each test to avoid odor residue. Gently place the mouse in the central area of ​​the Y-maze and immediately start recording. Recording time: 10 minutes. After the test, gently remove the mouse and return it to its cage. Clean the apparatus and wait 5 minutes before testing the next mouse (to avoid odor interference). Randomize the mice by cage number to avoid time sequence bias. Cross-test mice within the same group (e.g., alternating between control and experimental groups).

[0068] d. Results Analysis:

[0069] Mine result trajectory diagram as follows Figure 2 As shown;

[0070] The chart shows the timeline for entering the central area based on mine behavior. Figure 3 As shown;

[0071] Mining behavior studies entering the central area time bar chart as follows Figure 4 As shown;

[0072] Analysis of the Y-maze results is as follows: Figure 5 As shown;

[0073] Y-maze rotation ratio bar chart as follows: Figure 6 As shown;

[0074] Based on the information in the attached figures, the results of this direct-drinking powder experiment are as follows: Results Analysis: According to the results of the mine behavior analysis, the movement trajectory diagram of the sample treatment group was denser than that of the model group, and the time to enter the central area was longer than that of the model group. This indicates that the sample treatment group showed improved exploration and movement abilities compared to the model group, and the hippocampal dependence deficit in the three-turn mice was improved. According to the results of the Y-maze behavior analysis, the rotation ratio of mice in the sample treatment group was greater than that in the model group, indicating that the memory ability of the mice in this group was improved compared to the model group. The above results indicate that this Heberon phosphatidylserine taurine direct-drinking powder has a certain improving effect on Alzheimer's disease.

[0075] Example 2:

[0076] Reference Figure 1 As shown, this embodiment of the invention provides a method for preparing a phosphatidylserine taurine direct-drinking powder that improves memory and Alzheimer's disease, including the following steps:

[0077] Step 1: Boil and dry the resistant dextrin at 52°C for 1 hour, pass it through a 60-mesh sieve, and then divide it into small and large portions of 0.2 and 0.8 by mass. Mix erythritol, phosphatidylserine, tea theanine and polygonatum powder and pass them through a 60-mesh sieve.

[0078] Step 2: Boil and dry citric acid, menthol, and taurine at 52°C for 1 hour, then pass them through a 60-mesh sieve. Also pass vitamin B1 and vitamin B2 through a 60-mesh sieve.

[0079] Step 3: Mix the sifted erythritol, phosphatidylserine, tea theanine, polygonatum powder and a small amount of resistant dextrin from Step 1, and mix them in a mixer for 10 minutes to obtain Mixed Powder 1. Mix the sifted citric acid, menthol, taurine, vitamin B1 and vitamin B2 from Step 2 in a mixer for 20 minutes to obtain Mixed Powder 2.

[0080] Step 4: Place Mixed Powder 1 and Mixed Powder 2 in a mixer and mix for 20 minutes to obtain phosphatidylserine taurine direct drinking powder that improves memory and Alzheimer's disease. Pack the direct drinking powder into bags with a bag size of 1.0g / bag. After bagging, inspect and store the powder in the warehouse for sale.

[0081] Example 3:

[0082] This invention provides an application of phosphatidylserine taurine direct drinking powder for improving memory and Alzheimer's disease, used to improve poor memory and Alzheimer's disease patients.

[0083] Although embodiments of the invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A phosphatidylserine taurine direct-drinking powder for improving memory and Alzheimer's disease, characterized in that: It consists of the following raw materials by weight percentage: Erythritol 30%–40%; Resistant dextrin 28%–38%; Phosphatidylserine 12%-15%; Tea contains 5%–10% theanine; Polygonatum powder 2%–8%; Menthol 2%–6%; Citric acid 0%–1%; Taurine 0%–1%; Vitamin B1 0%–1%; Vitamin B2 0%–1%.

2. The phosphatidylserine taurine direct-drinking powder for improving memory and Alzheimer's disease according to claim 1, characterized in that: It consists of the following raw materials by weight percentage: 35% erythritol; 32% resistant dextrin; Phosphatidylserine 15%; Tea contains 7% theanine; Polygonatum powder 6%; Menthol 4%; Citric acid 0.96%; Taurine 0.03%; Vitamin B1 0.005%; Vitamin B2 0.005%.

3. A method for preparing a phosphatidylserine taurine direct-drinking powder for improving memory and Alzheimer's disease, wherein the phosphatidylserine taurine direct-drinking powder for improving memory and Alzheimer's disease is the phosphatidylserine taurine direct-drinking powder for improving memory and Alzheimer's disease described in claims 1-2, characterized in that: Includes the following steps: Step 1: Boil and dry the resistant dextrin at 52℃-55℃ for 1h-1.5h, pass it through a 60-mesh sieve, and then divide it into small and large portions of 0.2 and 0.8 by mass. Mix erythritol, phosphatidylserine, tea theanine and polygonatum powder and pass it through a 60-mesh sieve. Step 2: Boil and dry citric acid, menthol and taurine at 52℃-55℃ for 1h-1.5h, then pass them through a 60-mesh sieve. Also pass vitamin B1 and vitamin B2 through a 60-mesh sieve. Step 3: Mix the sifted erythritol, phosphatidylserine, tea theanine, polygonatum powder and a small amount of resistant dextrin from Step 1, and mix them in a mixer for 10 minutes to obtain Mixed Powder 1. Mix the sifted citric acid, menthol, taurine, vitamin B1 and vitamin B2 from Step 2 in a mixer for 20 minutes to obtain Mixed Powder 2. Step 4: Place Mixed Powder 1 and Mixed Powder 2 in a mixer and mix for 20 minutes to obtain phosphatidylserine taurine direct drinking powder that improves memory and Alzheimer's disease. Pack the direct drinking powder into bags with a bag size of 1.0g / bag. After bagging, inspect and store the powder in the warehouse for sale.

4. An application of a phosphatidylserine taurine direct-drinking powder for improving memory and Alzheimer's disease, wherein the phosphatidylserine taurine direct-drinking powder for improving memory and Alzheimer's disease is the phosphatidylserine taurine direct-drinking powder for improving memory and Alzheimer's disease mentioned in any one of claims 1-3, characterized in that: It is used to improve poor memory and for patients with Alzheimer's disease.

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