Method for implementing excessive accumulation of alpha-oxoglutarate by regulation and control of carbon metabolism flow with assist factor
A technology of ketoglutarate and cofactor, applied in the field of excessive accumulation of α-ketoglutarate
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Embodiment 1
[0033] Embodiment 1 improves vitamin B 1 Concentration increases pyruvate dehydrogenase (PDH) pathway metabolic flux
[0034] Vitamin B 1 When the concentration increased to 0.04mg / L, the production of α-KG reached a maximum of 10.3g / L, the production of pyruvate decreased to 58.6g / L, and the carbon molar ratio of pyruvate to α-KG (CPYR / CKG) was 3.9. Continue to increase the vitamin B in the medium 1 When the concentration is high, the production of α-KG will gradually decrease.
Embodiment 2
[0035] Example 2 Increasing the Biotin Bio Concentration Increases the Metabolic Flux of the Pyruvate Carboxylase (PC) Pathway
[0036] When the biotin Bio concentration in the medium increased to 0.06mg / L, the α-KG production reached the maximum value of 14g / L, the pyruvate production dropped to 52.6g / L, and the carbon molar ratio of pyruvate to α-KG (CPYR / CKG) was 3.74.
Embodiment 3
[0037] Example 3 Calcium Carbonate Effects on Carbon Metabolic Flow Direction and Flux Size
[0038] As shown in the table below, when the pH was not adjusted in shake flasks and fermenters, cell growth was weak and acid production was minimal. In shake flasks with CaCO 3 When used as a buffer, a higher concentration (6.8 g / L) of α-KG was produced. In fermentor culture, if the pH is adjusted with NaOH, the concentration of α-KG in the fermentation broth is very low. But if instead CaCO 3 When the pH was adjusted, the concentration of α-KG in the fermented liquid increased by 8 times compared with that when the pH was adjusted with NaOH. When the calcium carbonate concentration in the culture system was 60g / L, the accumulation of α-KG reached the maximum value (12.8g / L).
[0039] Fermentation controls in shake flasks and fermenters
[0040] cell
[0041] Description: C PYR / C KG It is the ratio of the number of carbon moles of pyruvate to the number of carbon...
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