42 results about "Torulopsis glabrata" patented technology

The invention discloses a method for increasing yield of pyruvic acid, belonging to the fermentation engineering field. The method is as follows: when Torulopsis glabrata (CCTCC NO: M202019) is used to ferment and produce pyruvic acid, aspartic acid is added to increase the acid stress resistance of Torulopsis glabrata. When the pH values are 5.5 and 4.5, the yields of pyruvic acid are separately55.8g / L and 30.4g / L which are separately increased by 12.4% (49.6g / L) and 48.4% (20.5g / L) than the control groups without aspartic acid; and the method has better technical effect and wide application prospect.

The invention discloses a method for microbial fermentative synthesis of α-ketoglutaric acid, in which calcium carbonate is added to a culture medium and the concentration of biotin is increased to promote the generation of a large amount of α-ketoglutarate during pyruvate fermentation. During the fermentative production of pyruvate by Torulopsis glabrata CCTCC M202019, delaying the addition of calcium carbonate inhibited the formation of α-KG and increased the carbon molar ratio of pyruvate to α-KG (C PYR / C KG ), and increasing the concentration of calcium carbonate in the medium will promote the accumulation of a large amount of α-KG, and when the concentration of calcium carbonate is 40g / L, it is most conducive to the formation of α-KG. Keep the concentration of calcium carbonate in the medium constant, increase the concentration of biotin in the medium, promote the concentration of α-KG to rise continuously and C PYR / C KG The value decreased continuously, and the accumulation of α-KG was 23.5g / L when the biotin concentration was 60μg / L. When there is Ca 2+ When present, the activity of intracellular pyruvate carboxylase can be increased by 40%, while the activity of pyruvate dehydrogenase system does not change significantly. Ca in medium 2+ The increase of the concentration of biotin and biotin can significantly increase the activity of pyruvate carboxylase, so that T. glabrata shifts from fermentative production of pyruvate to synthesis of high-concentration α-KG.

A constructing method for non-resistance badge auxotrophy smoothing ball false yeast belongs to biological engineering technology field. The invention is a system method for breeding large fragment deletion non-resistance badge auxotrophy smoothing ball false yeast comprising obtaining large fragment deletion consanguinity recombination fragments by blending PCR method, preparing high efficient sprout fungi electric conversion competence, concentrating candio-hermal and screening restrictive culture medium. Goal auxotrophy bacterial strain can be obtained quickly within 3 to 5 days by a suit system method. The deletion of large fragments avoids efficiently reversion and consanguinity recombination of exogenous plasmid. Furthermore, the method can be used many times in a same leaving strain so that multiplex auxotrophy for following genetic engineering study can be obtained because any resistance badge is not used in entire process.

The invention discloses a method for using the transcription factor Crz1p to regulate acid stress resistance of Toruula glabrata, belonging to the field of bioengineering. In the present invention, by deleting or overexpressing the Cgcrz1 gene of Torulopsis glabrata, the acid stress resistance of the strain is correspondingly reduced or improved. The present invention also compares the cell membrane fatty acid, sterol composition, ratio and permeability of the deletion mutant strain Cgcrz1Δ under acid stress. Acid stress resistance.

The present invention belongs to the field of bioengineering technology. The bacterium of the present invention is a kind of Torulopsis glabrata, WSH-, obtained with WSH-IP303 as starting strain and through NTG mutagenesis and breeding selection in culture medium with added acetic acid as replenishing carbon source. Compared with the starting strain, LQ307 has mush reduced pyruvate decarboxylase, and strong and stable pyruvate producing capacity. The yield of pyruvate reaches 46.2 g / L, 21% higher than that of starting strain when using acetic acid as replenishing carbon source and through shaking bottle culture for 48 hr; the yield of pyruvate may reach 68.7 g / L the glucose converting rate may reach 0.651 g / g through fermentation in a 5 L fermenting tank for 64 hr.

The invention discloses a method for increasing the concentration of flavor liquid fermentation components of a fen-favor liquor. The method comprises the following steps of adding a fen-favor high-temperature yeast into cooked and spread-cooled sorghum to stack; adding a liquefying enzyme and a saccharifying enzyme into a fermentation tank filled with sorghum flour paste, and adding the stacked sorghum to ferment for 2-4 days after liquefying and saccharifying; adding loofah sponge to ferment for 1.5-2.5 days; adding torulopsis glabrata to ferment for 1.5-2.5 days; connecting the fermentation tank and an overflow tower, and carrying out backflow fermentation on a fermentation mash between the fermentation tank and the overflow tower filled with carbon dioxide for 4-6 days; and stopping backflow, enabling all the fermentation mash to enter the fermentation tank, and then, adding the fen-favor high-temperature yeast to ferment for 2-4 days. A liquid-solid interface is increased by utilizing the loofah sponge, so that the formation quality of an ester substance is increased, and the flavor increasing aim is achieved; and meanwhile a gas-liquid interface is increased through the overflow of a fermentation liquid in the large overflow tower filled with carbon dioxide, and the formed flavor components are increased.

The invention belongs to the technical field of fermentation engineering and discloses a recombinant bacterium with a function of pyruvate production intensity improvement and application of the recombinant bacterium with the function of pyruvate production intensity improvement. By targeting expression of a mitochondrion pyruvate carrier protein to a cell membrane in a torulopsis glabrata strain, cell outgoing rate of pyruvate is increased while a feedback inhibition effect of the pyruvate on glycolytic pathway key enzymes is weakened, and consequently pyruvate yield is increased, and production intensity is improved. By means of genetic engineering technology, a signal peptide sequence Shrew1p and MPC1 derived from saccharomyces cerevisiae are positioned and integrated to a pY26 expression vector to construct a saccharomyces cerevisiae recombinant expression plasmid pY26-Shrew1p-MPC1 which is electrophoretically transferred into a recipient bacterium TgU to obtain an engineering strain TgU-(pY26-Shrew1p-MPC1) high in pyruvate yield. Compared with a reference strain TgU-(pY26), the engineering strain TgU-(pY26-Shrew1p-MPC1) has the advantage that dry cell weight, glucose consumption rate, pyruvate yield and pyruvate production intensity are increased by 58.3%, 68.1%, 154.4% and 152.6% respectively.

The invention discloses a method for improving the output of pyruvic acid by a fermentation method through adding gluconic acid sodium salt, which belongs to the fermentation preparation of pyruvic acid technical field. The method adopts a strategy that during the aerobic fermentation process of Torulopsis glabrata CCTCC M202019 with glucose as a substrate, 10 to 15g / L of the gluconic acid sodiumsalt is added as a mixed carbon source when the fermentation is performed for 12 to 16h, which ensures that the output of the pyruvic acid is improved by 11.16 percent and the production period is also reduced to 44h from 48h. The research thought has universal application significance to the production of various important fermented products in industry such as organic acid, amino acid and so on.

The invention discloses a method for regulating the osmotic stress of Torulopsis glabrata, belonging to the field of bioengineering. The invention regulates the ability of resisting osmotic pressure stress of Torulopsis glabrata by overexpressing or deleting the coding gene CgRDS2 of the native transcription factor. The results showed that the deletion of CgRDS2 gene in Torulopsis glabrata, compared with the wild-type strain, could reduce the biomass by 23%, cell viability by 46%, and cell membrane integrity by 47.6% under osmotic stress conditions; after overexpressing the CgRDS2 gene , under the stress condition of 1.5M NaCl, the biomass of the strain increased by 10%, the cell activity increased by 39%, and the cell membrane integrity increased by 12.1%.

The invention relates to a pyruvic acid and L-dopa co-production process and application. The process comprises the following steps: (1) obtaining a pyruvic acid feed liquid by fermentation with torulopsis glabrata, sterilizing the feed liquid by a ceramic membrane, and removing proteins, nucleic acids, pigments and other macromolecules by an ultrafiltration membrane; (2) adding a certain amount of catechol, ammonium acetate, EDTA, sodium sulfite and other compounds to a pyruvic acid concentrate according to the molar amount of pyruvic acid, adjusting the pH to 7.0-9.0, and preparing an enzyme-catalyzed substrate solution; (3) fermenting with tyrosine-phenol lyase genetic engineering bacteria to obtain a tyrosine-phenol lyase bacterial solution, centrifuging to collect thalli, breaking cells by a high-pressure homogenizer, centrifuging, and collecting an enzyme solution; and (4) adding a certain amount of substrate solution to the enzyme solution, stirring evenly, and at the temperature of 25 DEG C, carrying out seal shock reaction. The substrate solution is prepared from the pyruvic acid concentrate, and the concentration of catechol is controlled at 0-10 g / L by fed-batching the substrate solution, the product reaches 120 g / L or more, and fed-batch of the substrate solution is stopped; when the content of catechol in the reaction solution is less than 0.2 g / L, and the reactionis stopped.