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Torulopsis and method for producing acetonic acid by fermenting the same

A yeast and pyruvate technology, which is applied in the field of alanine-deficient pyruvate high-yielding bacteria, can solve the problems of high production cost, further evaluation of the safety properties of strains, strict production control requirements, etc., and achieve an increase in acid production rate, Increase sugar-acid conversion rate and reduce metabolism

Inactive Publication Date: 2009-05-27
上海新立工业微生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] A common defect of the technology reported in the above documents or patents is that the production cost is relatively high, the production control requirements are strict, and the safety properties of the strains need to be further evaluated.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1～3

[0063] Get a loop of DY404 bacteria from the fresh slant and inoculate it into 3 conical flasks with a volume of 5 L and a liquid capacity of 0.7 L, cultivate it at 30 ° C for 20 hours, and insert it into a 30 L fermenter with an inoculum of 10% by volume. The ventilation is 20L / min, the temperature is 30°C, and the stirring is 450r / min. During the process, 8.8mol / L NaOH is used to control the pH to 5.5.

[0064] The components of the seed culture solution are as follows:

[0065] Glucose 40g / L, peptone 5g / L, KH 2 PO 4 1g / L, MgSO 4 ·7H 2 O 0.3g / L.

[0066] The components of the fermentation medium are as follows:

[0067] Glucose 100g / L, ammonium sulfate 6g / L, KH 2 PO 4 2g / L, MgSO 4 ·7H 2 O 0.3g / L, CaCl 2 50mg / L, FeSO 4 40mg / L, CuSO 4 4mg / L, MnCl 2 2mg / L, ZnCl 2 5mg / L, thiamine hydrochloride 0.01mg / L, pyridoxine hydrochloride 0.5mg / L, biotin 0.02mg / L, niacin 7.5mg / L, hair powder 0.8g / L.

[0068] Cells and other proteins are then removed by membrane separa...

Embodiment 4～7

[0073] Take a ring of bacteria from a fresh slant and inoculate it into a 500mL shaker flask, incubate at 30°C for 20 hours, put the inoculum at 1% by volume into a 30L seed tank, cultivate for 15 hours, and inoculate 300L with an inoculum of 10% by volume In the fermenter, the ventilation is 8L / hour, the temperature is 30°C, and the stirring is 200r / min. During the process, 10mol / L NaOH is used to control the pH to 5.5.

[0074] Others are with embodiment 1. The results are shown in Table 2.

[0075] Table 2

[0076]

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PUM

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Abstract

The invention discloses Torulopsis glabrata and a method for fermenting to produce pyruvic acid by adopting a strain. The Torulopsis glabrata is an aspartic acid and lactamine defective pyruvate high yield strain, can reduce the metabolism of the pyruvic acid, and increase the conversion rate of sugar acid. In addition, in the Torulopsis glabrata, a transamination reaction from the pyruvic acid to lactamine exists, and a decarboxylic reaction from aspartic acid to the lactamine also exists. Compared with the prior art, when the strain is adopted to ferment for producing the pyruvic acid, the acid yield can be improved by 38 percent in the same production period, and the conversion rate can be improved by between 2 and 3 percent, so the strain has better prospect of industrialized production.

Description

technical field [0001] The invention relates to an alanine-deficient pyruvic acid high-yielding bacterium and a method for fermenting and producing pyruvic acid with the bacterium. Background technique [0002] Pyruvate is a very important organic acid, which is widely used in chemical, pharmaceutical, agricultural chemical and other industries and scientific research. [0003] The production of pyruvic acid by fermentation has many advantages, such as low cost of raw materials, wide range of sources, high product purity, and mild reaction conditions, which has aroused extensive interest of researchers. In terms of breeding high-yielding strains, the main focus is on blocking the metabolic outlet of pyruvate and accelerating the metabolism of glucose to pyruvate. Great progress has been made so far. [0004] Literature: Four vitamin auxotrophic T.glabrata reported by Yonehara ("J.Ferment.Bioeng".1994,78:155-159) and Miyata ("J.Ferment.Bioeng".1996,82:475-479) IF00005 (for ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/16C12N15/01C12P7/40C12R1/88
Inventor 章健关惠琴孙炳耀
Owner 上海新立工业微生物科技有限公司
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