9 results about "Transamination" patented technology

A production method according to one embodiment is a method for producing 4-(aminomethyl)cyclohexane-1-carboxylic acid from 1,4-bis(aminomethyl)cyclohexane using a protein consisting of an amino acid sequence having 60% or more identity to an amino acid sequence shown in any one of SEQ ID NOs. 1 to 4 and 103 to 105 and having transamination activity, and a protein consisting of an amino acid sequence having 50% or more identity to an amino acid sequence shown in any one of SEQ ID NOs. 19 to 22, 35 to 46 and 127 to 139 and having aldehyde dehydrogenase activity.

The present invention relates to a mutant transaminase, a nucleic acid encoding the mutant transaminase, a vector comprising the nucleic acid, a cell comprising the mutant transaminase or nucleic acid, and a method for enzymatic reductive transamination of a ketone and formation of a primary amine in the presence of a mutant transaminase.

The invention provides a biosynthesis method of an esdoxaban tosylate chiral intermediate. According to the preparation method, 3-cyclohexene-1-carboxylic acid methyl ester is taken as a starting raw material, and a target intermediate is finally obtained through carboxylesterase resolution, hydrolysis, hydroxylation, dehydrogenation oxidation, transamination reaction, amidation and Boc2O reaction in sequence. According to the method, high-selectivity carboxylesterase is used for realizing chiral resolution; p450 oxidase with excellent regioselectivity and stereoselectivity is utilized to realize specific hydroxylation of a C-H bond under a mild condition, and a traditional process depending on a highly toxic chemical oxidant is overturned; then, clean oxidation is realized through alcohol dehydrogenase, and a way is laid for subsequent reaction; most importantly, the transaminase is adopted to directly construct the chiral amine, so that the use of a high-risk reagent sodium azide is thoroughly avoided, and major potential safety hazards are eliminated from the source; and finally, green amidation is realized by virtue of lipase, and an acyl chloride method for generating corrosive wastewater is replaced.

The present application provides an amine dehydrogenase, which is obtained by mutating an amino acid dehydrogenase as shown in SEQ ID NO. 1, and the mutation comprises at least one mutation in the amino acids at positions 293, 69, 115 and 262 of SEQ ID NO. 1. The present application also provides the use of the amine dehydrogenase in preparing chiral amines. By means of protein engineering, the present application modifies the amino acid dehydrogenase catalyzing amino acid substrates into an amine dehydrogenase (AmDH) with ketone and aldehyde as substrates. The amine dehydrogenase of the present application has high reductive amination activity, and can be applied in the process of catalyzing transamination of hydroxy ketone and prochiral ketone compounds to prepare chiral amines, including but not limited to 3-hydroxy-D-tyrosine. The high catalytic activity, high yield and 99%+ stereoselectivity meet the requirements, and the present application has the advantages of green and efficient, low cost and high optical purity of products, and is one of the most ideal reactions for the synthesis of chiral amines.

The invention discloses a preparation method of L-echinacea A and an acid salt thereof. The preparation method of the L-echinacea A acid salt comprises the following synthetic route. And carrying out alkalization treatment on the L-inclusion canine A acid salt, so as to obtain the L-inclusion canine. According to the invention, pyridinium salt and chiral N-acryloyl oxazolidinone are taken as initial raw materials, a (2R, 5R, 6S) core skeleton of the obtuseline A is selectively constructed through a 1, 3-dipolar cycloaddition reaction, then introduction of all carbon atoms in the obtuseline A is completed through processes of hydrogenation reduction, transamination, hydroboration reduction reaction, methylation and the like, and the novel preparation method of the obtuseline A is realized. The preparation method comprises the following steps: by taking L-echinokimene as a raw material, carrying out protection group replacement, oxidation rearrangement reaction, protection group removal and other processes to prepare L-echinokimene acid salt, and carrying out alkalization treatment on the L-echinokimene acid salt to obtain the L-echinokimene with optical activity, the whole route is simple and convenient, the yield is good, the selectivity is relatively good, and the reaction conditions are mild and controllable.

The present invention relates to a mutant transaminase, a nucleic acid encoding the mutant transaminase, a vector containing the nucleic acid, a cell containing the mutant transaminase or nucleic acid, and a method for enzymatic reductive transamination of ketones and formation of primary amines in the presence of the mutant transaminase.

The invention discloses a method for catalytically synthesizing (S)-1-tert-butyloxycarbonyl-3-aminopyrrolidine by transaminase, which comprises the following steps: carrying out carbonyl transamination reaction on tert-butyloxycarbonyl-3-pyrrolidone in a reaction medium in the presence of transaminase, coenzyme and an amine donor to obtain high-purity (S)-1-tert-butyloxycarbonyl-3-aminopyrrolidine, meanwhile, the production cost is reduced.

The application discloses a method for preparing furfurylamine by in-situ extraction fermentation, and belongs to the technical field of bio-chemical industry. In order to solve the problems of strong raw material and product inhibition effect, high concentration of by-products and low concentration of product in the preparation of furfurylamine by biological transamination catalysis, the application provides a method for preparing furfurylamine by in-situ extraction fermentation, specifically, fermentation culture and induction are carried out on a strain, then furfural, an amino donor and glucose are added in a flow manner to carry out a transamination reaction, and an external flow addition of an extractant is carried out to carry out extraction fermentation. The application firstly reduces the generation of by-products by using the biological fermentation technology, simultaneously couples the extraction separation technology to separate furfurylamine in-situ, effectively solves the problems of biological toxicity and multiple side reactions faced by the biological transamination, and realizes the biological fermentation of high-concentration furfurylamine.