The invention discloses an LC-MS / MS positive
ion mode detection method of EDTA in food (particularly
wine). The method comprises the steps that 1, a sample to be detected is pretreated, wherein 11, the sample to be detected is sucked into a container capable of being closed, a 20.0 mg / L
boric acid solution and a 10% amine
methanol solution (preferentially, a 10% butyl amine
methanol solution) are added, and the container is closed for even mixing; 12, the solution obtained through the step 11 passes through a microporous filtering film with the
pore diameter being 0.1-0.5
micrometer to obtain a filtrate; 2, the filtrate is placed into a sample
bottle, then, an amine
methanol solution is added, sufficient and even shaking is carried out, and then detection is carried out through liquid chromatogram and tandem
mass spectrum. The method can qualitatively and quantitatively analyze and detect EDTA in food such as
wine, and is good in stability, accurate and sensitive, the qualitative limit is 0.0001 mg / kg, and the quantitative limit is 0.001 mg / kg; the detection method is simple, method errors are avoided, consumed time is short,
energy consumption is low, and the application and popularization prospect is good.