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A kind of peptoid and its preparation method and application

A kind of synthetic method technology, applied in the field of biomedicine, can solve the problems of lack of accuracy and specificity of the method, and achieve the effect of good specificity, low cost and simple synthesis

Active Publication Date: 2020-09-08
THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are also some other detection methods, such as brain atrophy can be seen in imaging examinations, such as enlargement of the lateral ventricle and third ventricle; low metabolic areas of the parietal lobe can be seen in PET, SPECT, and MRI; early loss of α rhythm and lower potential in EEG examination, Diffuse slow waves are common, and the degree of EEG slowing is related to the severity of dementia; cerebrospinal fluid detects the increase of tau protein and β-amyloid protein, etc. These methods have some limitations
[0005] For example, CN 106018827 A discloses a detection marker for Alzheimer's disease and its detection method. The provided set of serum differential proteins can be used for detection of Alzheimer's disease, but the current method lacks accuracy and specificity, and Certain pathological features need to be present in the patient to be detected

Method used

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  • A kind of peptoid and its preparation method and application
  • A kind of peptoid and its preparation method and application
  • A kind of peptoid and its preparation method and application

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Experimental program
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Embodiment 1

[0044] The preparation of embodiment 1 class peptide

[0045] The peptoids of the present invention are synthesized by solid-phase subunit synthesis, and the method comprises the following steps:

[0046] (1) Add 2M bromoacetic acid and 3.2M N,N'-diisopropylcarbodiimide (DIC) to Rink amide AM resin (substitution level 0.3mmol / g), react at 37°C for 30min, and the resin terminal Amino acylation;

[0047] (2) Add 2M primary amine and react at 37°C for 90 minutes to replace the bromine atom through a nucleophilic substitution reaction to complete the synthesis of a subunit;

[0048] (3) Steps (1) and (2) are repeated until the synthesis of the remaining units is completed;

[0049] (4) After the synthesis is completed, the side chain protecting group is removed, and the peptoid is cleaved from the resin with 95% trifluoroacetic acid, 2.5% ultrapure water, and 2.5% triisopropylsilane for future use.

[0050] The molecular formula of the prepared peptoid is as follows:

[0051] ...

Embodiment 2

[0053] The preparation of embodiment 2 class peptides

[0054] The peptoids of the present invention are synthesized by solid-phase subunit synthesis, and the method comprises the following steps:

[0055] (1) Add 2M bromoacetic acid and 3.2M N,N'-diisopropylcarbodiimide (DIC) to Rink amide AM resin (substitution level 0.3mmol / g), react at 37°C for 30min, and the resin terminal Amino acylation;

[0056] (2) Add 2M primary amine and react at 37°C for 90 minutes to replace the bromine atom through a nucleophilic substitution reaction to complete the synthesis of a subunit;

[0057] (3) Steps (1) and (2) are repeated until the synthesis of the remaining units is completed;

[0058] (4) After the synthesis is completed, the side chain protecting group is removed, and the peptoid is cleaved from the resin with 95% trifluoroacetic acid, 2.5% ultrapure water, and 2.5% triisopropylsilane for future use.

[0059] The molecular formula of the prepared peptoid is as follows:

[0060]...

Embodiment 3

[0062] The preparation of embodiment 3 class peptides

[0063] The peptoids of the present invention are synthesized by solid-phase subunit synthesis, and the method comprises the following steps:

[0064] (1) Add 2M bromoacetic acid and 3.2M N,N'-diisopropylcarbodiimide (DIC) to Rink amide AM resin (substitution level 0.3mmol / g), react at 37°C for 30min, and the resin terminal Amino acylation;

[0065] (2) Add 2M primary amine and react at 37°C for 90 minutes to replace the bromine atom through a nucleophilic substitution reaction to complete the synthesis of a subunit;

[0066] (3) Steps (1) and (2) are repeated until the synthesis of the remaining units is completed;

[0067] (4) After the synthesis is completed, the side chain protecting group is removed, and the peptoid is cleaved from the resin with 95% trifluoroacetic acid, 2.5% ultrapure water, and 2.5% triisopropylsilane for future use.

[0068] The molecular formula of the prepared peptoid is as follows:

[0069]...

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Abstract

The invention relates to the technical field of biomedicine, in particular to peptoid. The invention in particular relates to the peptoid as well as a preparation method and application thereof. The peptoid is prepared from ethylenediamine, 4-phenylbenzylamine, beta-phenylethylamine, tetramethylene diamine, 3,4-methylenedioxy benzylamine, isobutylamine, R(+)-alpha-methylbenzylamine, glycine and 3-aminopropionic acid subunit. The peptoid provided by the invention has high sensitivity for beta-amyloid protein in serum, and can be used for effectively distinguishing the serum of a patient and the serum of a normal person by identifying the beta-amyloid protein in the serum, thus providing a new choice for diagnosis and monitoring of Alzheimer's disease.

Description

technical field [0001] The present invention relates to the field of biomedical technology, in particular to a peptoid, in particular to a peptoid and its preparation method and application, in particular to a peptoid capable of detecting the content of β-amyloid protein in serum and its preparation method and application. Background technique [0002] Alzheimer's disease (Alzheimer's Disease, AD) is a neurodegenerative disease with high incidence in the elderly with progressive memory loss and cognitive dysfunction caused by misfolding and abnormal accumulation of amyloid peptides. . According to the "2015 Global Alzheimer's Report" released by Alzheimer's Disease International (ADI) in 2015, it is estimated that there are currently about 46.8 million dementia patients in the world. This number will gradually increase at a rate of doubling every 20 years, and is expected to reach 74.7 million by 2030 and 131.5 million by 2050. This estimate represents a 12%-13% increase f...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/00C07K1/06C07K1/04G01N33/68A61K38/16A61P25/16
CPCA61K38/00C07K14/00G01N33/6896G01N2800/2821
Inventor 朱凌高厚乾赵子健杨延莲胡志远刘长亮刘明珠
Owner THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA
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