10 results about "Amino Acylation" patented technology

At least the presence of a starting material is detected by measuring glycine formed as a by-product along with a main product through a chemical reaction involving hippuric acid and / or methylhippuric acid as the starting material and catalyzed by a hydrolase. The hydrolase is an aminoacylase having an amino acid sequence of SEQ ID NO: 1 or a protein having the amino acid sequence of SEQ ID NO: 1 in which one or more amino acids are deleted, replaced, or added and having activity for hydrolysis of the hippuric acid and the methylhippuric acid.

The invention relates to the technical field of insulin derivatives, in particular to a long-acting acylated insulin derivative and application thereof. The long-acting acylated insulin derivative is formed by acylating and connecting a fatty acid side chain and an insulin peptide chain, and the fatty acid side chain is acylated and connected with epsilon amino of amino acid K on the insulin peptide chain. The insulin derivative provided by the invention has excellent hypoglycemic ability, the action time is remarkably prolonged, the insulin derivative can be administrated once a week, the compliance and sugar control willingness of a patient can be remarkably improved, and the insulin derivative has a wide market prospect.

The present invention relates to an isolated polypeptide having amino acylase activity and comprising an amino acid sequence having at least 87% sequence identity over its full length to the amino acid sequence set forth in SEQ ID NO: 1. The invention further relates to isolated nucleic acid molecules comprising a nucleotide sequence encoding such an aminoacylase, plasmid vectors comprising said nucleic acid molecules, recombinant host cells comprising isolated nucleic acid molecules or vectors, and methods of producing said aminoacylase. The invention also encompasses the use of an aminoacylase according to the invention for the N-acylation of an amino acid or a salt thereof, as well as a method for producing an N-acylamino acid or a salt thereof using an aminoacylase. Furthermore, the present invention relates to the N-acylamino acids obtained, to compositions comprising them, and to the use of the N-acylamino acids produced in cosmetics, home care products or industrial and / or institutional products.

The application discloses a preparation method of 2-acetylamino-4-[(2-hydroxyethyl)sulfonyl]benzoic acid. The 2-acetylamino-4-[(2-hydroxyethyl)sulfonyl]benzoic acid is prepared from 4-fluoro-2-nitrobenzoic acid methyl ester as a starting material through aromatic nucleophilic substitution reaction, nitro reduction reaction, ester hydrolysis reaction, amino acylation reaction, thioether oxidation reaction and the like. The method has the advantages of mild reaction condition, high yield, low cost, easy availability of raw materials, and the like. The synthesis process meets the requirements of green chemistry, the synthesis route is innovative, the reaction post-treatment is simple, and the product is easy to purify.

The invention relates to the technical field of organic synthesis, in particular to a preparation method of an N-BOC-aniline derivative, which takes an aniline derivative and di-tert-butyl dicarbonate as reaction substrates, sodium hydride as a reaction reagent, 4-dimethylaminopyridine as a catalyst and tetrahydrofuran solvent as a solvent to carry out amino acylation reaction to obtain the N-BOC-aniline derivative. In the amino acylation reaction process, the temperature is controlled to be 20-30 DEG C, the rotating speed is controlled to be 400-500 r / min, the obtained reaction liquid is purified to finally obtain the N-BOC-aniline derivative, the aniline derivative is 2-bromine-5-chloroaniline, 4-hydroxyaniline, 3-hydroxyaniline, 4-carboxyl aniline, 3-carboxyl aniline or 4-bromine-6-carboxyl aniline, and the N-BOC-aniline derivative is 2-bromine-5-chloroaniline, 4-hydroxyaniline, 3-hydroxyaniline, 4-carboxyl aniline, 3-carboxyl aniline or 4-bromine-6-carboxyl aniline. The preparation method has the characteristics of simple preparation process, low preparation cost and high yield and purity of the final N-BOC-aniline derivative, the final yield can reach 84-93%, and the purity can reach 98.98-100%.

The invention belongs to the field of plant gene function research, and relates to application of aminoacylase-1 in improving the nitrogen utilization efficiency of plants and a method for improving the nitrogen utilization efficiency of the plants through the aminoacylase-1. Experimental research shows that the overexpression ZmACY-1 gene promotes the growth and development of the plant and the absorption of external nitrate radicals, the nitrogen accumulation of the plant is influenced, and the nitrogen accumulation amount of the overexpression strain is higher than that of a wild type and a knockout strain.

The application provides a method for catalytically synthesizing (S)-2-amino-3-(thiazole-4-yl)propionic acid, taking 2-acetylamino-3-(thiazole-4-yl)propionic acid as a substrate, wherein the catalyst contains aminoacylase, and the aminoacylase comprises an amino acid sequence as shown in at least one sequence of SEQ ID NO:2, SEQ ID NO:4 or SEQ ID NO:5. In the method, the catalyst has the advantages of simple preparation process, low cost, mild reaction condition, short technological process, high product yield and pure stereoisomer, and has high industrial application potential.

The present invention provides a novel amino acid active ester for use in the aminoacylation of a tRNA, said ester being capable of reducing safety and waste risks. The present invention provides a compound represented by formula (I): or a salt thereof, in particular dichloropyridinyl methyl ester and 2,2,2-trifluoroethyl ester or a salt thereof.

The invention belongs to the technical field of chemical pharmacy, and particularly relates to a synthesis method of flomoxef axetil, which comprises the following steps: carrying out substitution reaction on a compound II tert-butyl alcohol and sodium hypochlorite in an organic solvent to generate a compound III tert-butyl hypochlorite; the compound I and a compound III tert-butyl hypochlorite are subjected to a methoxylation reaction to generate a compound IV; removing an amino protecting group from the compound IV under the action of amino acylase to obtain a compound V; reacting the compound VI potassium difluoromethyl mercaptoacetate under the action of hydrochloric acid to generate a compound VII; the compound V reacts with a compound VII (difluoromethyl mercaptoacetic acid) to obtain a target compound VIII; the method is simple and reliable in reaction operation, short in reaction route, green and environment-friendly, high in yield and easy in post-treatment, and high-purity flomoxef axetil is obtained.

The invention belongs to the technical field of bioengineering, and particularly relates to an amino acylase mutant and application of the amino acylase mutant in preparation of D-3, 4-dimethoxy phenylalanine. The amino acid sequence of the amino acylase mutant is one of SEQ ID NO: 4, SEQ ID NO: 6, SEQ ID NO: 8, SEQ ID NO: 10, SEQ ID NO: 12 or SEQ ID NO: 14. The compound disclosed by the invention has high hydrolytic activity and high D type selectivity on N-acyl-DL-3, 4-dimethoxy phenylalanine.