5 results about "Amino Acylation" patented technology

The present invention relates to an isolated polypeptide having amino acylase activity and comprising an amino acid sequence having at least 87% sequence identity over its full length to the amino acid sequence set forth in SEQ ID NO: 1. The invention further relates to isolated nucleic acid molecules comprising a nucleotide sequence encoding such an aminoacylase, plasmid vectors comprising said nucleic acid molecules, recombinant host cells comprising isolated nucleic acid molecules or vectors, and methods of producing said aminoacylase. The invention also encompasses the use of an aminoacylase according to the invention for the N-acylation of an amino acid or a salt thereof, as well as a method for producing an N-acylamino acid or a salt thereof using an aminoacylase. Furthermore, the present invention relates to the N-acylamino acids obtained, to compositions comprising them, and to the use of the N-acylamino acids produced in cosmetics, home care products or industrial and / or institutional products.

The application discloses a preparation method of 2-acetylamino-4-[(2-hydroxyethyl)sulfonyl]benzoic acid. The 2-acetylamino-4-[(2-hydroxyethyl)sulfonyl]benzoic acid is prepared from 4-fluoro-2-nitrobenzoic acid methyl ester as a starting material through aromatic nucleophilic substitution reaction, nitro reduction reaction, ester hydrolysis reaction, amino acylation reaction, thioether oxidation reaction and the like. The method has the advantages of mild reaction condition, high yield, low cost, easy availability of raw materials, and the like. The synthesis process meets the requirements of green chemistry, the synthesis route is innovative, the reaction post-treatment is simple, and the product is easy to purify.

The invention belongs to the field of plant gene function research, and relates to application of aminoacylase-1 in improving the nitrogen utilization efficiency of plants and a method for improving the nitrogen utilization efficiency of the plants through the aminoacylase-1. Experimental research shows that the overexpression ZmACY-1 gene promotes the growth and development of the plant and the absorption of external nitrate radicals, the nitrogen accumulation of the plant is influenced, and the nitrogen accumulation amount of the overexpression strain is higher than that of a wild type and a knockout strain.

The application provides a method for catalytically synthesizing (S)-2-amino-3-(thiazole-4-yl)propionic acid, taking 2-acetylamino-3-(thiazole-4-yl)propionic acid as a substrate, wherein the catalyst contains aminoacylase, and the aminoacylase comprises an amino acid sequence as shown in at least one sequence of SEQ ID NO:2, SEQ ID NO:4 or SEQ ID NO:5. In the method, the catalyst has the advantages of simple preparation process, low cost, mild reaction condition, short technological process, high product yield and pure stereoisomer, and has high industrial application potential.

The invention belongs to the technical field of bioengineering, and particularly relates to an amino acylase mutant and application of the amino acylase mutant in preparation of D-3, 4-dimethoxy phenylalanine. The amino acid sequence of the amino acylase mutant is one of SEQ ID NO: 4, SEQ ID NO: 6, SEQ ID NO: 8, SEQ ID NO: 10, SEQ ID NO: 12 or SEQ ID NO: 14. The compound disclosed by the invention has high hydrolytic activity and high D type selectivity on N-acyl-DL-3, 4-dimethoxy phenylalanine.