57 results about "Ferrocenecarboxylic acid" patented technology

The invention discloses a preparation method of a ferrocenyl carbon nanotube composite material and application thereof and belongs to the technical field of synthesis of inorganic materials. The preparation method of the composite material comprises the following steps: firstly carrying out oxidization and amination on carbon nanotubes and carrying out acylation on ferrocenecarboxylic acid, then taking triethylamine as an acid-binding agent in a dichloromethane solvent, and enabling the carbon nanotubes subjected to amination to react with activated ferrocenylformyl chloride to prepare the composite material. The preparation method of the composit material is simple and is mild in reaction condition; the product is convenient to separate and purify and stable in structure. The composite material takes the carbon nanotubes with large specific surface area and high conductivity as support materials; ferrocenyl derivatives which can be used as electron media and provided with excellent electrochemical reversibility are covalently coupled on the surfaces of the carbon nanotubes, so that the composite material is excellent in effect of redox reaction for electrochemically catalyzing dopamine; the common interference matters including ascorbic acid and uric acid have no interference on detection of dopamine; the composite material is wide in linear response range and high in sensitivity and repeatability for detection of dopamine.

The invention discloses a preparation method of a molecular imprinted polymer for detecting lung cancer tumor markers. The method comprises the following basic process of: dissolving template molecules (ferrocenecarboxylic acid-tumor marker), a functional monomer and a cross-linking agent in an organic solvent (pore-foaming agent); then transferring the solution into water, stirring and emulsifying; adding an initiator for cross-linking, performing ultraviolet polymerization to obtain spherical molecular imprinted substances with relatively uniform particle sizes; and washing by ethanol, and centrifuging to remove the template molecules to obtain the molecular imprinted polymer with specificity to the lung cancer tumor markers. The molecular imprinted polymer is also used as an identifying element of a biosensor. The invention provides a new tumor diagnosis method.

The invention discloses a novel method to prepare spherical covalent framework material (COFETTA-TPAL) nano composite (Fc-(COOH)2@COFETTA-TPAL) with electroactive ferrocenecarboxylic acid molecules (Fc-(COOH)2). Tetra-(4-aminobenzene)ethylene (ETTA), terephthalaldehyde (TPAL) and Fc-(COOH)1 are co-dissolved in 1,4-dioxane solvent before ultrasonic mixing; acetic acid is added; the mixed solution is transferred to a reactor and allowed to react in an oven at 120 DEG C for 2 days so as to obtain earthy yellow solid Fc-(COOH)2@COFETTA-TPAL nano composite. The Fc-(COOH)2@COFETTA-TPAL nano composite prepared via solvothermal one-pot synthesis is spherical, with uniform size and diameter of about 200 nm. Fc-(COOH)2 Molecules are well loaded in holes of COFETTA-TPAL. Crystal structure of the COFETTA-TPAL is well maintained, and electroactivity of Fc-(COOH)2 and COFETTA-TPAL is retained. Studies discover that the nano composite can catalyze disproportionation reaction of H2O2 and may replace peroxidase, the preparation process is simple, and the materials are low in price and easy to obtain.

The invention discloses an Fc-FF-RGD composite. The Fc-FF-RGD composite is characterized in that the composite is a polypeptide analogue sequence composed of ferrocenecarboxylic acid, biphenyl alanine and arginine-glycine-aspartic acid. Compared with the prior art, the Fc-FF-RGD composite has the following advantages that an RGD ligand on hydrogel can be combined with alpha5beta1 integrin on the surfaces of cells so that adhesion and multiplication of the cells are promoted and a good biological nano material application potential is shown; and furthermore, self-assembled nano small balls can identify the alpha5beta1 integrin on the surfaces of cells, so that the Fc-FF-RGD composite can be used as a drug transportation carrier to realize the aim of enhancing the anti-tumor effect.

The invention discloses a gold nanorod biotic compound and a preparation method and applications thereof and belongs to the technical field of electrochemical analysis. A gold nanorod modified by silicon dioxide (AuNRs@SiO2) is used as a carrier and combined with a detection antibody (dAb) and ferrocenecarboxylic acid (Fc) to prepare dAb-AuNR-Fc which is used for amplifying escherichia coli in an electrochemical immunoassay dairy product. A 'sandwich' immunoassay model is established on basis of specificity interaction between the escherichia coli and escherichia coli antibody, and Fc combined with an electrode surface is measured by a differential pulse voltammetry to obtain a current signal. According to the gold nanorod biotic compound and the preparation method and the applications thereof, the electrochemical immunoassay method based on dAb-AuNR-Fc biotic compound is used for escherichia coli detection, the detection sensitivity, the specificity and the accuracy are high, and a novel method is provided for analysis and research of the escherichia coli in the dairy product.

The invention discloses a dual-valve multi-stimulation-responsive drug carrier constructed on the basis of mesoporous silicon / cyclodextrin / zinc oxide quantum dots and a preparation method thereof. With mesoporous silicon nanoparticles as a carrier, after drug loading, blocking is performed by using aminated zinc oxide quantum dots to obtain a drug carrier blocked by the zinc oxide quantum dots; then, after the drug carrier blocked by the zinc oxide quantum dots is connected with ferrocenecarboxylic acid through chemical bonds, the drug carrier further reacts with anhydrided cyclodextrin for secondary blocking to obtain a composite nanometer drug carrier system. The composite nanometer drug carrier system has the properties of dual pH sensitivity, H2O2 oxidation sensitivity and fluorescenceimaging, has more efficient targeting drug administering ability in cancer cells, and can effectively increase the drug utilization rate.

The invention provides a polycarbonate composite material for 3D printing and a preparation method thereof. The preparation method of the polycarbonate composite material comprises the following steps: mixing N-aminoethyl-gamma-aminopropyl triethoxy silane with ethyl acrylate, adding ethyl methacrylate, then sequentially adding cyclodextrin and ferrocenecarboxylic acid, then adding polycarbonate particles, heating, stirring and cooling, wherein the content of polycarbonate is 40-50%, the content of N-aminoethyl-gamma-aminopropyl triethoxy silane is 5-20%, the content of ethyl acrylate is 5-20%, the content of ethyl methacrylate is 10-30%, the content of cyclodextrin is 0.5-2%, and the content of ferrocenecarboxylic acid is 0.5-2%. The preparation method of the polycarbonate composite material for 3D printing is simple in technology, and the prepared polycarbonate composite material can be used for carrying out 3D printing in a temperature range of 50-70 DEG C, can not block a nozzle of a 3D printer and can be applied to the fields of automobile manufacturing, medical devices, aviation, aerospace, electronics and the like.

The invention relates to the technical field of energy material synthesis, and discloses a lattice-distorted ultrathin metal organic framework nanosheet catalyst and a preparation method and application thereof. The preparation method comprises the following steps: dissolving terephthalic acid and ferrocenecarboxylic acid in a mixed solution of DMF, ethanol and water, adding cobalt salt and an acid-binding agent, ultrasonically stripping, washing and separating to obtain the lattice-distorted ultrathin metal organic framework nanosheet catalyst. The ultrathin two-dimensional nanosheet structure obtained by lattice distortion caused by ferrocenecarboxylic acid and ultralong-time ultrasonic treatment has a larger specific surface area, more active sites are exposed, the oxygen evolution reaction can be promoted, and the nanosheet structure has good catalytic stability, and has the advantages of low cost, high utilization rate, strong pertinence, good effect and the like.

The invention discloses a chromium-free passivation treatment agent used before coating of a cast aluminum alloy workpiece. The chromium-free passivation treatment agent is prepared from raw materials in percentage by weight as follows: 5-10% of potassium persulfate, 1-5% of sorbitol, 0.5-1.5% of ammonium meta-vanadate, 0.1-0.5% of sodium diisobutyl naphthalene sulfonate, 0.1-0.5% of potassium zirconium carbonate, 0.05-0.08% of ferrocenecarboxylic acid, 0.05-0.08% of lauryl alcohol polyoxyethylene, 0.01-0.05% of sodium pyrrolidone carboxylic acid and the balance of water. Compared with the prior art, the chromium-free passivation treatment agent has the benefits that poisonous and harmful content such as hexavalent chromium and the like is not contained, and the environment-friendliness is realized; the corrosion resistance and the adhering force of a passivation film formed on the surface of the cast aluminum alloy workpiece reach a chromating technique level, and a chromating technique can be completely replaced; the treatment process is simple, the treatment time is short, construction is convenient and the treatment agent is suitable for large-scale industrial production.

The invention discloses an electrochemical immunosensor and a preparation method thereof, and application in preparation of a PSA detection kit, and belongs to the technical field of electrochemical immunosensors. The electrochemical immunosensor comprises an operating electrode, the operating electrode comprises a modified electrode whose platinum electrode surface is wrapped by a graphene oxide / polyion liquid composite material layer, and an antibody 1 and an antibody 2 in specific binding with a to-be-analyzed target object, the antibody 1 is fixed to the graphene oxide / polyion liquid composite material layer, and the antibody 2 is marked with ferrocenecarboxylic acid. According to the electrochemical immunosensor, a novel graphene oxide / polyion liquid compound is provided and modifiedon the bare platinum electrode, a sandwich immunity mode is adopted to prepare the electrochemical immunosensor, the ferrocene-marked antibody 2 has a signal amplification effect, and the lowest detection limit of the sensor used for PSA detection reaches 1.0 pg / mL.

The invention discloses a preparation method of a ferrocene-based azo-phenyl amphiphilic segmented copolymer material for preparing a regular honeycomb membrane. The preparation method comprises the following steps: preparing chlorocarbonyl ferrocene from ferrocenecarboxylic acid, and then preparing ferrocenyl methoxyethyl methacrylate monomers from chlorocarbonyl ferrocene and hydroxyethyl methacrylate; preparing azo-phenyl-6-hydroxyl from 4-hydroxyl azobenzene, and then preparing azobenzene monomers from azo-phenyl-6-hydroxyl; preparing a macromolecular initiator from polyethylene glycol monomethyl ether; mixing the ferrocenyl methoxyethyl methacrylate monomers, the azobenzene monomers and the macromolecular initiator, and adding CuBr and PMDETA, thus obtaining the regular honeycomb membrane errocene-based azo-phenyl amphiphilic segmented copolymer material (PEO-b-P(FcEMA-co-MAAZOOHEE)s. The polymer hydrophilic-hydrophobic chain segment ratio is flexible to adjust, the material heatstability is good, the stimulation response phenomenon is apparent, and convenience in photoelectric response detection can be realized.

The invention relates to a modulated nickel / cobalt bimetal organic framework (MOF)-based electrocatalyst, a preparation method and application. The catalyst comprises a conductive substrate and a ferrocenecarboxylic acid-modulated nickel / cobalt bimetallic MOF (NiCoMOF-Fc) material loaded on the surface of the conductive substrate, wherein the NiCoMOF-Fc material has catalytic activity. The catalyst is prepared by a simple one-step solvothermal method and comprises the following specific steps: mixing an N, N-dimethylformamide (DMF) solution in which terephthalic acid and ferrocenecarboxylic acid are dissolved and a DMF solution in which nickel salt and cobalt salt are dissolved in a reaction kettle in which a conductive substrate is placed, carrying out solvothermal reaction, taking out the conductive substrate after the reaction is finished, washing and drying to obtain the catalyst. The catalyst is obtained. The modulated nickel / cobalt bimetal MOF-based electrocatalyst provided by the invention has excellent urea oxidation reaction (UOR) electrocatalytic activity, the preparation process is simple, large-scale production is easy, and the modulated nickel / cobalt bimetal MOF-based electrocatalyst has important practical application value in urea-assisted water decomposition, urea-containing wastewater treatment and direct urea fuel cells.

The invention provides a method for determining the glucose in an aqueous solution by using a TiO2 nano-tube modified ito electrode. The method comprises: synthesizing TiO2 nano-tubes by using a hydrothermal method, grafting -OH on the surface, modifying an ito electrode simultaneously with the TiO2 nano-tubes and catechol by using an electrochemical method to form a composite film, modifying thesurface of the composite film with ferrocenecarboxylic acid, coating the surface of the outer layer of the ito electrode with glucose oxidase in a dropwise manner, and determining the glucose contentin the aqueous solution by using a chronoamperometry method. According to the present invention, the electrode is modified by coupling the TiO2 nano-tubes with the catalytic activity and the catechol,and the ferrocenecarboxylic acid synergistically acts as the electron mediator of glucose oxidase, such that the electron transfer and reaction signal during the glucose determination is enhanced, and the method has significant advantages in the determination of the trace sample.

The invention provides an acrylamide type monomer containing a dicyclopentadienyl iron structure, as well as an amphipathic polymer, a preparation method and a use thereof. The preparation method of the monomer comprises three steps of reactions: firstly enabling ferrocenecarboxylic acid to react with aliphatic diamine to generate an amino-containing dicyclopentadienyl iron compound, then reacting with 4-bromo-1-butyric acid tert-butyl ester to introduce a carboxylic ester structure, and finally reacting with acryloyl chloride to get the acrylamide type monomer containing the dicyclopentadienyl iron structure. An acrylamide type polymer containing the dicyclopentadienyl iron structure is obtained by free radical polymerization or active free radical polymerization of the monomers, and the acrylamide type amphipathic polymer containing the dicyclopentadienyl iron structure is generated by performing selective hydrolysis on the obtained polymer under the action of trifluoroacetic acid. The amphipathic polymer can form a self-assembled aggregate with a microstructure in a selective solvent and can be further applied to chemical modification of an electrode, and thus has potential applications in the fields of catalysis, optoelectronics, molecular recognition, sensors and the like.

The invention discloses a lithium-based anti-bacteria metal surface treatment agent which comprises the following components in parts by weight: 15-20 parts of polyvinyl alcohol, 0.4-0.5 part of tea saponin, 3-4 parts of lithium lubricating grease, 0.4-1 part of ferrocenecarboxylic acid, 0.1-0.2 part of hexahydrophthalic anhydride, 0.1-0.12 part of DBNPA, 0.5-1 part of potassium sorbate, 0.1-0.2 part of hexamethyl phosphoramide, 1-2 parts of sodium bisulfite, 6-8 parts of sericin, 0.08-0.1 part of boric acid, 10-20 parts of methylbenzotriazole and 180-200 parts of deionized water. According to the invention, water-soluble polymer polyvinyl alcohol and sericin are mixed, and through modification of boric acid, tensile strength and heat stability of a blended membrane are improved, the membrane adsorbed on the surface of the metal is more uniform and stable since corrosion inhibition alkali liquor using methylbenzotriazole as a main material is added, the metal material is protected from being corroded by harmful media in atmosphere and water, a better corrosion inhibition effect is achieved, and bacterial and fungal contamination can be effectively resisted sine the added DBNPA, potassium sorbate and other various metal surface treatment agent additives are compatible.

The invention belongs to the technical field of electrochemistry, and discloses a Co-Fe alloy / graphene compound material, as well as a preparation method and application thereof. The method comprises the following steps: ultrasonically dispersing graphene oxide into water; stirring and dissolving 1,1'-ferrocenecarboxylic acid and cobalt acetate tetrahydrate in the graphene oxide dispersion liquid at room temperature; controlling the temperature to be 20-80 DEG C, adding alkali into the prepared solution under a stirring state, adjusting the pH value of the solution to be 4-12, and stirring for 2-10 hours; transferring the obtained system into a hydrothermal reactor, performing hydrothermal treatment for 48-72 hours at 40-200 DEG C, cooling to room temperature, taking out the reaction liquid, separating to obtain precipitate, and drying the precipitate; grinding, and calcining the ground powder under nitrogen atmosphere at 400-600 DEG C for 2-8 hours to obtain the Co-Fe alloy / graphene compound material. The Co-Fe alloy / graphene compound material prepared by the method is used as an electrode material of a super capacitor, and the electrochemical performance of the capacitor is greatly promoted, so that the Co-Fe alloy / graphene compound material has a wide development prospect.

The invention discloses a ferrocenecarboxylic acid modified unsaturated polyester resin and a synthesis method thereof. The resin is characterized in that the unsaturated polyester resin with molecular chain ends containing ferrocene group structures is prepared by carrying out melt polycondensation on a ferrocenecarboxylic acid monomer, fumaric acid and dihydric alcohol. The resin prepared by the invention has obvious advantages of better flowability, easily controlled solidification and formation, high hot-strength of products, high dimensional accuracy and good performance stability and can be used in a bonder which has higher requirements for performance indexes and is used for bonding a permanent magnet.

An amperometric sensor for uric acid and a manufacturing method thereof are disclosed, in which polyacrylamide is used to fix catalase, uricase and ferrocenecarboxylic acid on a working electrode. In determining concentration of uric acid, hydrogen peroxide is produced when enzyme and uric acid react with each other and then a reduction current generated from enzyme on the electrode with an external voltage 200 mV applied is detected. In determining concentration of uric acid, a concentration range of 2.5-20 mg / dl is achieved and sensibility of the sensor in a linear portion is 5.17 uAcm−2(mg / dl)−1. In addition, reaction time required for the reaction between enzyme and uric acid is 5.17 uAcm−2(mg / dl)−1.

The invention relates to a preparation method of colloidal carbon spheres loaded with magnetic Fe3O4 nanoparticles on the surface. The method comprises the following steps of: dispersing ferrocenecarboxylic acid into absolute ethyl alcohol; adding an obtained mixture into a reaction kettle; putting the reaction kettle into a controlled-temperature furnace, heating to 140-180 DEG C, and reacting for 4-28 hours; taking the container out; naturally cooling to room temperature to obtain a black product; and washing and centrifugally separating to obtain a needed product. Process parameters are adjustable, an entire preparation process is easy and convenient to operate, conditions are easy to control, and post-treatment of a product is convenient, so that large-scale production is easy, and side products causing pollution to the environment are not produced during preparation; the method is accordant with a sustainable development requirement, and is an environmentally-friendly synthesis process; obtained colloidal carbon spheres serving as a product have high purity and high monodispersity, and are in nanoscale sizes; and the loaded magnetic nanoparticles have adjustable loading capacity, superparamagnetic property and high application potentials in targeting medicaments and separation technologies.

The invention discloses PET (polyethylene terephthalate) / montmorillonite nano composite special for engineering plastic, manufactured from, by weight, 58-60 parts of PET resin, 20-22 parts of polyvinylidene fluoride, 5-7 parts of montmorillonite, 1.4-1.8 parts of nano alumina, 6-8 parts of carbon fiber, 0.7-0.9 part of zinc powder, 0.1-0.2 part of benzotriazole, 7-9 parts of gypsum fiber, 1.8-2.5 parts of ferrocenecarboxylic acid, 6-8 parts of methyldiethanolamine, 1.5-1.8 parts of dodecyl trimethyl ammonium chloride, and suitable water; by combining with the advantages in PET resin and polyvinylidene fluoride, the properties of the composite, such as corrosion resistance, wear resistance and impact resistance, are improved; by combining the added carbon fiber and zinc powder to the materials, the prepared composite has good electrical conductivity, significant antistatic effect, light weight and high strength; when used in engineering plastic, the composite has the properties meeting normal use, is easier to cut, and is highly worthy of marketing and application.

The invention provides a ferrocene zinc porphyrin organic dye, a preparation method of a ferrocene zinc porphyrin organic dye sensitized solar cell and application of the ferrocene zinc porphyrin organic dye. According to the ferrocene zinc porphyrin organic dye, the preparation method of the ferrocene zinc porphyrin organic dye sensitized solar cell and the application of the ferrocene zinc porphyrin organic dye, nitrobenzaldehyde, ferrocenecarboxaldehyde, ferrocenecarboxylic acid, and porphyrin are used as raw materials, and the ferrocene zinc porphyrin organic dye is successfully synthesized through a multi-step reaction of condensation, reduction, coordination, substitution and the like. A nano-crystalline TiO2 / FTO (Fluorine-Doped Tin Oxide) electrode is sensitized through a soaking method; a ferrocene zinc porphyrin complex dye sensitized solar cell is researched; a result shows that the dye-sensitized solar cell prepared by the invention, which is irradiated by standard light, has different capacity of outputting an electric current due to different ferrocene, has different extents of photoelectric conversion efficiency, and has incident photon to current conversion efficiency (IPCE) which can be up to 85 percent at most. According to the ferrocene zinc porphyrin organic dye, the preparation method of the ferrocene zinc porphyrin organic dye sensitized solar cell and the application of the ferrocene zinc porphyrin organic dye, a synthetic process is simple, a process route is short, and the operation is convenient; a reaction condition is mild, the cost is low, and the yield is high.

An amperometric sensor for uric acid and a manufacturing method thereof are disclosed, in which polyacrylamide is used to fix catalase, uricase and ferrocenecarboxylic acid on a working electrode. In determining concentration of uric acid, hydrogen peroxide is produced when enzyme and uric acid react with each other and then a reduction current generated from enzyme on the electrode with an external voltage 200 mV applied is detected. In determining concentration of uric acid, a concentration range of 2.5-20 mg / dl is achieved and sensibility of the sensor in a linear portion is 5.17 uAcm−2(mg / dl)−1. In addition, reaction time required for the reaction between enzyme and uric acid is 5.17 uAcm−2(mg / dl)−1.

The invention belongs to the technical field of waste water treatment, and discloses a Co-Fe alloy loaded carbon material and a preparation method and application thereof. The Co-Fe alloy loaded carbon material is structurally characterized in that a carbon layer coats the outer surface of Co-Fe alloy; the inside of the Co-Fe alloy is hollow. Under the condition of room temperature, 1,1'-ferrocenecarboxylic acid and cobalt acetate tetrahydrate are stirred and dissolved into water; the temperature is controlled to be 20 to 80 DEG C; alkali is added into the obtained solution during stirring; the pH of the solution is regulated to 4 to 12; then, 1,10'-phenanthroline is added; the stirring is performed for 1 to 3h; the materials are transferred into a hydrothermal reaction kettle; the hydrothermal reaction is performed for 48 to 72h at 40 to 200 DEG C; separation is carried out to obtain solids; drying is performed on the solids; grinding is performed; the ground powder is roasted for 2 to 8 hours at 200 to 800 DEG C in the nitrogen gas atmosphere; the Co-Fe alloy loaded carbon material can be obtained. The Co-Fe alloy loaded carbon material obtained through preparation has very good adsorption performance; meanwhile, the reaction conditions are simple; the operation is easy; the yield is high; the industrial production is easy; the whole process is clean; no pollution is caused; no harm is caused on the environment; the separation from water can be easily realized.

The invention belongs to the technical field of photocatalytic hydrogen production, and relates to a preparation method of functional carbon quantum dot modified Ag-In-Zn-S quantum dots, which comprises the steps of: mixing CDs and a 1M ferrocenecarboxylic acid solution using dimethyl sulfoxide as a solvent, adding distilled water to a constant volume of 20 mL, and carrying out a hydrothermal reaction at a temperature of 110-140 DEG C for 2-4 h to obtain a CDs-FcA precursor; and mixing silver nitrate, indium nitrate, zinc acetate and L-cysteine into an aqueous solution, adjusting the pH value of the solution to 8.5 with 1M NaOH, adding the CDs-FcA precursor and thioacetamide, conducting ultrasonic stirring to a uniform state, carrying out a hydrothermal reaction at the temperature of 110 DEG C for 2-4 h, and conducting centrifugal washing after the reaction is finished, so as to obtain the unctional carbon quantum dot modified Ag-In-Zn-S quantum dots. The high visible light response capacity of the Ag-In-Zn-S quantum dots is utilized, photo-generated holes in the quantum dots can be rapidly extracted through a two-order low HOMO energy level track, the recombination efficiency of photo-generated charges is greatly reduced, and higher water photolysis hydrogen production efficiency is achieved. The method is simple in process, low in cost, easy to obtain, convenient for batch production, non-toxic and harmless, and meets the requirement for environment-friendliness.

A phthalocyanine dye is prepared through reaction of the halogenated metal-phthalocyanine complex on ferrocenylcarboxylic acid under existance of bicyclohexyl biimide carbonate and 4-dimethylamino pyridine. A phthalocyanine dye composition contains tetra-(2,4-dimethyl-3-phenyloxy) copper phthalocyanine bromidid (1-10 wt.%), bis-(hydroxymethyl) tetra-(2,4-dimethyl-3-phenyloxy) copper phthalocyanine bromidid (1-10 wt.%), bis-(hydroxymethyl) tetra-(2,4-dimethyl-3-phenyloxy) copper phthalocyanine, and esterified ferrocenylcarboxylic acid.

The invention discloses a composite nano material based on ferrocene and hypericin as well as a preparation method and application of the composite nano material. The preparation method comprises the following steps: adding polyethylene glycol polylysine, ferrocenecarboxylic acid N-succinimide ester and hypericin into a tetrahydrofuran medium, and self-assembling the product to form the composite nano material based on ferrocene and hypericin. The prepared composite nano material based on ferrocene and hypericin has the advantages of being high in local concentration, good in anti-tumor curative effect, rapid in kidney clearance, high in biocompatibility, small in toxic and side effects and the like. The good anti-tumor curative effect is reflected in that a large amount of active oxygen is generated, glutathione on the local part of a tumor is depleted, ferroptosis and hypericin-mediated photodynamic therapy generate a synergistic effect, meanwhile, immunogenic cell death is triggered, and the powerful anti-tumor effect is achieved.

The invention discloses a preparation method of ferrocenecarboxylic acid. The preparation method comprises the following steps: 1) feeding; 2) synthesis; 3) hydrolysis: adding a part of crushed ice and deionized water into a hydrolysis kettle, then carefully transferring the reaction mixed solution into the hydrolysis kettle for 30-60 minutes, using nitrogen for isolation during the period, continuously adding the crushed ice, keeping the hydrolysis internal temperature to be lower than 30 DEG C, and continuously stirring for 2-3 hours to ensure complete hydrolysis; 4) filtering and washing; and 5) washing. According to the invention, the nitrogen is introduced in the hydrolysis process to isolate oxygen; and the crushed ice is fed in batches, and the temperature of the hydrolysis kettle is continuously controlled, so that the materials are prevented from being oxidized before hydrolysis, and the generation of impurities is reduced.

The present invention discloses a method for constructing an electrochemical immunosensor by using the multi-walled carbon nanotube imitation mussel chemical modification to synthesize functional nano-composite probes. The method comprises: wrapping a polydopamine layer on the surface of a multi-walled carbon nanotube by using the imitation mussel excitation chemistry; after the polydopamine layeris grafted with the bromine initiator, using light-induced electron transfer-atom transfer radical polymerization and initiation on the surface of the polydopamine layer to form polymethylacrylic acid-glycidyl ester with a controllable molecular weight; using the amidogen to modify the polymethylacrylic acid-glycidyl ester to synthesize a functional nano-composite, and loading a large amount of electrochemically labeled anthraquinone dicarboxylic acid and ferrocenecarboxylic acid on the functional groups of the functional nano-composite to prepare two kinds of nano-composite probes for signalamplification; and finally, using the two functionalized nano-composite probes to prepare an electrochemical immunosensor, and performing electrochemical analysis and combined detection on two tumormarkers of the carcinoembryonic antigen and the alpha-fetoprotein.

The invention relates to a preparation method of ferrocenecarboxylic acid, comprising the following steps of: firstly, adding polyethylene glycol, iodine-potassium iodide and acetylferrocene into an aqueous alkali with uniformly stirring, wherein the acetylferrocene undergoes an iodoform reaction to form ferrocene carboxylic acid salt dissolved in a non-organic phase; secondly, extracting the solution obtained from the reaction of the first step by the use of an organic solvent, adjusting the pH value of a water phase to 2-3 by the use of strong acid to change sodium ferrocene carboxylic acid into ferrocenecarboxylic acid precipitated from the water phase. According to the invention, non-organic aqueous alkali is used as a solvent to prepare ferrocenecarboxylic acid. The preparation method provided by the invention is easy to control. During the process, the mild reaction is carried out in a solution with the reaction temperature being from zero to 5 DEG C for a short time; ferrocenecarboxylic acid can be produced only within 2 hours. The used raw materials are commonly sold in the market and are cheap. The yield of the product in the invention can reach more than 85% by optimum technology.