The invention belongs to the technical field of electrochemical immunoassay, and relates to an assembly array type multi-channel capacitance immune sensor based on a glass capillary structure, which is formed through the following steps: fixing antibody A (antigen A) on the inner wall of a glass capillary through aminosilane reagent and glutaraldehyde, inserting a platinum wire electrode into the glass capillary, fixing the platinum wire electrode in the glass capillary, injecting nonspecific protein, sealing active adsorption sites, washing, drying, and combining two glass capillaries formed through the above steps, thereby obtaining an immune electrode pair sensitive to A. Then an immune electrode pair sensitive to B, an immune electrode pair sensitive to C and an immune electrode pair sensitive to D are formed in sequence in the identical method. The assembly array type multi-channel capacitance immune sensor can be obtained through assembling the electrode pairs. A corresponding immune reaction substance is cultured and washed in the glass capillary and is then inserted in substrate solution with urinary enzyme, and the urinary enzyme labeled antigen (urinary enzyme labeled antibody) catalyzes the reaction of the substrate, thereby increasing the capacitance of the solution. Then the content of the antigen (antibody) can be measured.