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Electrochemical immunosensor and preparation method thereof, electrochemical immunoassay method and kit

A technology of immunosensor and analysis method, applied in the field of electrochemical immunoassay method and kit, electrochemical immunosensor and preparation, which can solve problems such as difficult to find

Active Publication Date: 2020-04-07
SOUTHWEST UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, ECL multiplex immunoassays still face great challenges because it is difficult to find two luminescent substances with potential resolution, no energy transfer, and co-reactive reagents that do not interfere with each other.
Although existing researchers have utilized different approaches and provided many feasible strategies to address the multiplexed immunoassay problem, they all exhibit certain drawbacks

Method used

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  • Electrochemical immunosensor and preparation method thereof, electrochemical immunoassay method and kit
  • Electrochemical immunosensor and preparation method thereof, electrochemical immunoassay method and kit
  • Electrochemical immunosensor and preparation method thereof, electrochemical immunoassay method and kit

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Embodiment 1

[0048] One, the following are described for the substances involved in the embodiments of the present invention:

[0049] Electrochemiluminescence, abbreviated as: ECL;

[0050] N-(4-aminobutyl)-N-ethyl isoluminol, abbreviated as: ABEI;

[0051] Graphite phase carbon nitride nanosheets, abbreviated as: g-C 3 N 4 ;

[0052] Brain natriuretic peptide, abbreviated as: BNP;

[0053] Cardiac troponin I, abbreviated as: cTnI;

[0054] Glassy carbon electrode, abbreviated as: GCE;

[0055] Gold nanoparticles, abbreviated as: AuNPs;

[0056] Electrodeposit gold nanoparticles on the surface of the glassy carbon electrode, abbreviated as: DpAu / GCE;

[0057] Bovine serum albumin, abbreviated as: BSA;

[0058] AuNPs@ABEI; anti-cTnI 2Combined with AuNPs@ABEI secondary antibody marker, abbreviated as: anti-cTnI 2 -AuNPs@ABEI;

[0059] Gold nanoparticles and graphitic carbon nitride nanosheets compounded with the first antibody complex, abbreviated as: AuNPs@g-C 3 N 4 ;anti-BNP ...

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Abstract

The invention discloses an electrochemical immunosensor and a preparation method thereof, an electrochemical immunoassay method and a kit. The electrochemical immunosensor is characterized in that gold nanoparticles are electrically deposited on the surface of a polished glassy carbon electrode, and two antibodies, namely anti-cTnI1 and anti-BNP1, are immobilized on the surface of the glassy carbon electrode. In the ECL detection process, two secondary antibody markers, namely anti-cTnI2-AuNPs(at)ABEI and anti-BNP2-AuNPs(at)g-C3N4, are synchronously assembled on the surface of an electrochemical immunosensor incubated with BNP and cTnI antigens, and along with the increase of the concentrations of cTnI and BNP of a sandwich layer, an ECL signal enhanced along with the increase of the antigen concentration is displayed, so that the synchronous multiple immunoassay of BNP and cardiac troponin I (cTnI) is realized, and an effective scheme is provided for the diagnosis of acute myocardialinfarction.

Description

technical field [0001] The invention relates to the technical field of biomedical detection, in particular to an electrochemical immunosensor, a preparation method, an electrochemical immunoassay method and a kit. Background technique [0002] Acute myocardial infarction (AMI) is a fatal disease that mainly kills the elderly. The World Health Organization (WHO) predicts that by 2030, cardiovascular disease will cause 23.3 million deaths per year. For most patients, some prodromal symptoms will appear 1-2 weeks before the onset of AMI. Therefore, early and accurate diagnosis of AMI markers can significantly reduce the risk of death. The currently reported diagnostic methods for AMI are usually based on a single biomarker, which inevitably leads to many misdiagnoses. [0003] Multiplex immunoassay (MIA) means that two or more target proteins are detected simultaneously in one assay. It has significant advantages over single-component immunoassay methods, including higher sa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/74G01N33/68G01N27/26G01N27/48G01N27/30
CPCG01N27/26G01N27/308G01N27/48G01N33/6893G01N33/74G01N2333/4712G01N2333/58G01N2800/324G01N2800/60
Inventor 陈时洪杜佳炜赵晋文谭兴容
Owner SOUTHWEST UNIVERSITY
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