An electrochemical immunosensor and preparation method, electrochemical immunoassay method and kit

Abstract

The invention discloses an electrochemical immunosensor, a preparation method, an electrochemical immunoassay method and a kit. The electrochemical immunosensor is as follows: gold nanoparticles are electrodeposited on the surface of a polished glassy carbon electrode, and anti-cTnI is immobilized 1 and anti-BNP 1 two antibodies. In the process of ECL detection, the anti-cTnI 2 -AuNPs@ABEI and anti-BNP 2 -AuNPs@g-C 3 N 4 Two kinds of secondary antibody markers were synchronously assembled on the surface of the electrochemical immunosensor incubated with BNP and cTnI antigen. As the concentration of cTnI and BNP in the sandwich layer increased, it showed an enhanced ECL signal with the increase of antigen concentration, thus realizing the brain Simultaneous multiplex immunoassay of natriuretic peptide (BNP) and cardiac troponin I (cTnI) provides an effective protocol for the diagnosis of acute myocardial infarction.

Description

technical field [0001] The invention relates to the technical field of biomedical detection, in particular to an electrochemical immunosensor, a preparation method, an electrochemical immunoassay method and a kit. Background technique [0002] Acute myocardial infarction (AMI) is a fatal disease that mainly kills the elderly. The World Health Organization (WHO) predicts that by 2030, cardiovascular disease will cause 23.3 million deaths per year. For most patients, some prodromal symptoms will appear 1-2 weeks before the onset of AMI. Therefore, early and accurate diagnosis of AMI markers can significantly reduce the risk of death. The currently reported diagnostic methods for AMI are usually based on a single biomarker, which inevitably leads to many misdiagnoses. [0003] Multiplex immunoassay (MIA) means that two or more target proteins are detected simultaneously in one assay. It has significant advantages over single-component immunoassay methods, including higher sa...

AI Technical Summary

Problems solved by technology

However, ECL multiplex immunoassays still face great challenges because it is difficult to find two luminescent substances with potential resolution, no energy transfer, and co-reactive reagents that do not interfere with each other.

Although existing researchers have utilized different approaches and provided many feasible strategies to address the multiplexed immunoassay problem, they all exhibit certain drawbacks

Images