Preparation method and application of immunosensor based on dopamine biomemetic modification
An immunosensor and dopamine technology, which is applied in the field of preparation of enzyme-free electrochemical immunosensor electrodes, can solve the problems of complex electrode modification steps and poor reproducibility, and achieve the effect of solving difficult synthesis, easy operation, and simple steps
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Embodiment 1
[0023] (1) Dopamine biomimetic modification: pipette 3 μL of freshly prepared 0.5 mg·mL -1 1. Dopamine hydrochloride solution with a pH of 8.5 was drip-coated on the surface of the treated and activated 3 mm glassy carbon electrode, and dried at room temperature;
[0024] (2) In situ preparation of nanoparticles: Rinse the electrode prepared in step (1) with ultrapure water several times, and then pipette 3 μL of 0.5 mg·mL -1 The silver nitrate solution is drip-coated on the surface of the electrode prepared in step (1), and dried at room temperature;
[0025] (3) Antibody immobilization: Rinse the electrode prepared in step (2) with ultrapure water several times, and apply the carcinoembryonic antigen (CEA) antibody solution on the surface of the electrode prepared in step (2) with a pipette gun, at 4°C dry under condition;
[0026] (4) Non-specific site blocking: Rinse the electrode prepared in step (3) with ultrapure water several times, and use a pipette gun to drop-coat...
Embodiment 2
[0030] (1) Biomimetic modification of dopamine: pipette 5 μL of freshly prepared 0.3 mg·mL -1 1. Dopamine hydrochloride solution with a pH of 8.5 was drip-coated on the surface of the treated and activated 4 mm glassy carbon electrode, and dried at room temperature;
[0031] (2) In situ preparation of nanoparticles: Rinse the electrode prepared in step (1) with ultrapure water several times, and then pipette 5 μL of 1.0 mg·mL -1 The chloroplatinic acid solution is drip-coated on the surface of the electrode prepared in step (1), and dried at room temperature;
[0032] (3) Antibody immobilization: Rinse the electrode prepared in step (2) with ultrapure water several times, and use a pipette gun to apply the prostate-specific antigen (PSA) antibody solution onto the surface of the electrode prepared in step (2), 4 Dry at ℃;
[0033] (4) Non-specific site blocking: Rinse the electrode prepared in step (3) with ultrapure water several times, and use a pipette gun to drop-coat th...
Embodiment 3
[0037] (1) Biomimetic modification of dopamine: Use a pipette to pipette 10 μL of the newly prepared concentration of 0.1 mg·mL -1 1. Dopamine hydrochloride solution with a pH of 8.5 is drip-coated on the surface of the treated and activated 5 mm gold electrode, and dried at room temperature;
[0038] (2) In situ preparation of nanoparticles: Rinse the electrode prepared in step (1) with ultrapure water several times, and then pipette 10 μL of 3.0 mg·mL -1 Palladium chloride solution is drip-coated on the electrode surface that step (1) makes, room temperature dries;
[0039] (3) Antibody immobilization: Rinse the electrode prepared in step (2) with ultrapure water several times, and apply the alpha-fetoprotein (AFP) antibody solution on the surface of the electrode prepared in step (2) with a pipette gun, at 4°C dry under condition;
[0040] (4) Non-specific site blocking: Rinse the electrode prepared in step (3) with ultrapure water several times, and use a pipette gun to ...
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