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Indium sulfide nanoparticle modified labeled electrochemical immunosensor and electrochemical immunoassay method thereof

An immunosensor and nano-microsphere technology, which is applied to the electrochemical variables of materials, material analysis through electromagnetic means, scientific instruments, etc., can solve the problem that metal sulfide nanomaterials rarely fix proteins, etc.

Active Publication Date: 2021-02-02
YANGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, metal sulfide nanomaterials have rarely been reported for immobilizing proteins in electrochemical biosensing

Method used

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  • Indium sulfide nanoparticle modified labeled electrochemical immunosensor and electrochemical immunoassay method thereof
  • Indium sulfide nanoparticle modified labeled electrochemical immunosensor and electrochemical immunoassay method thereof
  • Indium sulfide nanoparticle modified labeled electrochemical immunosensor and electrochemical immunoassay method thereof

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Embodiment 1

[0035] In this embodiment, a CEA-labeled electrochemical immunosensor based on hollow rambutan-like indium sulfide nanostructure microspheres is first prepared. The preparation method of the CEA-labeled immunosensor includes the following steps:

[0036] Preparation of hollow rambutan-like indium sulfide nanostructure microspheres

[0037] In a 50 mL beaker, add 0.0210 g of trimellitic acid, 0.3806 g of thiourea, 0.5 mL, indium nitrate, 9.5 mL of twice distilled water, and 0.0365 g of CTAB respectively, and place them on a magnetic stirrer to stir rapidly, until the homogeneously mixed clear solution , transfer the solution into the lining of the reaction kettle made of polytetrafluoroethylene, then put the inner lining into the reaction kettle made of steel material and tighten the upper cover of the reaction kettle, and then place the reaction kettle in a constant temperature blast drying oven to set The temperature was 120 °C and the reaction time was 12 h. After the react...

Embodiment 2

[0046] Using the CEA-labeled electrochemical immunosensor of the hollow rambutan-like indium sulfide nanostructure microspheres in Example 1 to carry out immunoassay methods includes:

[0047] (1) Incubate the CEA-labeled immunosensor prepared above in incubation solutions containing CEA antigen standard samples of different known concentrations, wherein the incubation solution consists of a 2:1:22 CEA antigen solution, HRP-labeled CEA antibody solution and PBS, wherein the concentration of HRP-labeled CEA antibody solution is 2 μg / mL, the incubation time is 40 min, and then washed with phosphate buffer solution; wherein, the CEA antigen solution in the incubation solution Concentrations are 0.01 ng / mL 0.1 ng / mL, 4 ng / mL, 5 ng / mL, 10 ng / mL, 13 ng / mL, 21 ng / mL, 23ng / mL;

[0048] (2) Using the CEA-labeled immunosensors prepared in the examples as the working electrode, the saturated calomel electrode as the auxiliary electrode, and the platinum sheet electrode as the counter ele...

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Abstract

The invention relates to an indium sulfide nanoparticle modified labeled electrochemical immunosensor and an electrochemical immunoassay method thereof in the technical field of electrochemical immunoassay, and the method comprises the following steps: firstly, synthesizing hollow rambutan-shaped indium sulfide nanostructure microspheres, biologically functionalizing the hollow rambutan-shaped indium sulfide nanostructure microspheres by using streptavidin, and modifying the hollow rambutan-shaped indium sulfide nanostructure microspheres on the surface of a glassy carbon electrode; through the specific affinity effect of streptavidin on biotin, fixing a biotinylated antibody is fixed on a functional interface, and bovine serum albumin is used for sealing to obtain the labeled electrochemical immunosensor. Indium sulfide has a large specific surface area and excellent biocompatibility, and streptavidin has high selectivity on biotinylated antibodies, so that captured antibodies can beeffectively fixed on the surface of indium sulfide. An electrochemical immunosensor prepared from the nano-microsphere can be used for quickly, simply and conveniently realizing high-sensitivity marking detection on tumor markers in serum or body fluid in a thionine solution system.

Description

technical field [0001] The invention relates to the technical field of electrochemical immunoassay, in particular to a labeled electrochemical immunosensor modified by indium sulfide nanometer microspheres and an electrochemical immunoassay method thereof. Background technique [0002] In 1990, Henry et al. proposed the concept of immunosensor, which is a new type of biosensor that combines highly sensitive sensing technology with specific immunoassay technology to detect antigen-antibody reactions. Tumor markers refer to substances produced by tumor cells and related to the properties of tumor tissues. These substances exist in the nucleus, cytoplasm and membrane of tumor tissues and tumor cells or are secreted in body fluids. The presence or content changes of tumor markers can indicate the existence and stage of tumors. Electrochemical immunosensor combines electrochemical analysis technology with immunoassay technology, and has attracted much attention due to its advant...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/48G01N27/327G01N27/30G01N33/53G01N33/574
CPCG01N27/48G01N27/308G01N27/3278G01N33/5302G01N33/57473
Inventor 杨占军曹佳雯李娟
Owner YANGZHOU UNIV
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