High-flux detection system of multianalyte simultaneous detection and electrochemical immunoanalytical method

A detection system, electrochemical technology, applied in the direction of material electrochemical variables, measuring devices, analysis materials, etc., can solve the problem of limiting the miniaturization of multi-component sensors, achieve full analysis control, facilitate miniaturization, and simple equipment Effect

Inactive Publication Date: 2007-08-22
NANJING UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These methods all overcome signal interference by enlarging the electrode spacing, but this method largely limits the miniaturization of multicomponent sensors.

Method used

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  • High-flux detection system of multianalyte simultaneous detection and electrochemical immunoanalytical method

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Experimental program
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Effect test

Embodiment 1

[0041] Example 1 further illustrates the high-throughput detection system for simultaneous detection of multiple analytes in conjunction with accompanying drawing 1: the high-throughput detection system for simultaneous detection of multiple analytes consists of a solution delivery part (1), a multi-channel immunosensor array (2) , a flow detection cell (3), an electrochemical detector (4) and a computer (5), wherein the solution transmission part (1) is composed of component A containing carrier fluid and H 2 o 2 The glass test tube of component B, the multi-channel peristaltic pump (6), the connecting pipe (7), the injection valve (8) and the waste liquid part (9), one end of the connecting pipe (7) leads to the group containing the carrier liquid Divide A and keep H 2 o 2 In the glass test tube of component B, the other end is respectively connected with the multi-channel peristaltic pump (6), the injection valve (8) and the waste liquid part (9); the multi-channel immuno...

Embodiment 2

[0042] Embodiment 2 Electrochemical immunoassay method for simultaneous detection of multiple analytes:

[0043] 1) Prepare a disposable electrode array on a PVC film by screen printing technology, as shown in Figure 3, it consists of eight carbon working electrodes a1, a2, a3, a4, a5, a6, a7, a8; a carbon counter electrode b; an Ag / AgCl reference electrode c; silver wire d and insulating film e; the production process is as follows: first print the silver conductive layer on the PVC film, and then print the carbon paste on the working electrode and the carbon counter electrode to form Carbon working electrode and carbon counter electrode, followed by printing Ag / AgCl paste on the position of reference electrode to form Ag / AgCl reference electrode, and finally printing green oil insulating film. The distance between adjacent working electrodes is 0.5 mm.

[0044] 2) Preparation of toluidine blue-chitosan biocompatible film. The chitosan powder was ultrasonically dissolved in...

Embodiment 3

[0053] Taking carcinoembryonic antigen (CEA), alpha-fetoprotein (AFP), ovarian cancer-associated antigen (CA125) and human chorionic gonadotropin (hCG) as examples to illustrate the electrochemical immunoassay method and high-throughput method for simultaneous detection of multiple analytes Application of detection system.

[0054] 75ng / mL CEA, 500ng / mL AFP, 500U / mL CA125, 400mIU / mL hCG standard solution were mixed with the prepared toluidine blue-chitosan solution at 1:3 (V:V) and heated at 4°C Set aside for 12 hours. 1 μL of each of the above mixed solutions was drop-coated on the surfaces of the eight working electrodes, and dried at room temperature for 2-3 hours. Store in a dry place at room temperature until use.

[0055] Simultaneously optimize the measurement conditions for the four systems, choose the best detection potential -0.5V, incubation time 40min, incubation temperature room temperature, pH value 6.5, injected H 2 o 2 The concentration is 10.7mM, and the d...

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Abstract

A high-flux detection system and the electrochemistry immune analytical method to detect many analytes at the same time has provided in the invention. It uses the functional chitosan membrane modified by the medium to fix the antigen molecule combining with the competitive immune analytical method, so the antigen in electrode surface competes with the antigen in sample to combine with the enzyme labeled antibody in warm cultivating solution to link the part antibody into the electrode surface, which catalyses the reaction between the H2O2 and the Toluidine Blue fixed in the electrode surface to get the detected antigen content in solution indirectly by the current catalyzed by the H2O2 oxidation. So the electrical medium is fixed in the electrode surface to avoid the signal cross generated by the diffusion which is the immune sensor array without disturbance. Combining with the flowing injection technology, it reaches the high-flux detection output. The sensor array is simple and cost little, also it has the short analyzing time and high sensitivity, so it is important to the life analytical chemistry, the immune science and the clinical medicine.

Description

1. Technical field [0001] The invention is a high-throughput detection system and an electrochemical immunoassay method for simultaneous detection of multiple analytes, involving a simultaneous immunoassay method for multiple components in the same sample, the combined use of electrochemical immunoassay and flow analysis technology, and a certain time In-house high-throughput detection output. 2. Background technology [0002] Immunoassay is a highly sensitive and selective method for detecting antibodies, antigens or related substances by using the highly specific reaction between antigen / antibody, which provides a powerful means for clinical immunoassay of serum tumor markers . However, in view of the unsatisfactory sensitivity and specificity of existing tumor markers, in actual tumor diagnosis, it is often necessary to improve the accuracy of diagnosis through joint analysis of multiple tumor markers. [0003] At present, multiple parallel single-component analysis is ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/53G01N27/416G01N27/27G01N35/00
Inventor 鞠熀先吴洁
Owner NANJING UNIV
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