Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Electrochemical immunoassay method for bisphenol A detection

An immune analysis and electrochemical technology, applied in the direction of material electrochemical variables, biological testing, material inspection products, etc., can solve the problems of long detection time, expensive instruments, and difficulty in real-time on-site detection, achieving fewer reaction steps and easy access , cheap effect

Inactive Publication Date: 2016-10-12
TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY +3
View PDF2 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In view of this, the present invention aims to propose an electrochemical immunoassay method for the detection of bisphenol A, to overcome the problems of traditional instrument detection and enzyme-linked immunoassay method for long detection time, expensive instruments, and difficulty in real-time on-site detection.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Electrochemical immunoassay method for bisphenol A detection

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Synthesis of Bisphenol A

[0031] Weigh 0.5-2.0g of bisphenol A and 0.5-2.5g of potassium carbonate and dissolve in 5-15mL of anhydrous dimethylformamide, and add 0.5-1.5g of ethyl 4-bromobutyrate. The reaction system was stirred at 65-80° C. for 8-16 hours. Then the reaction mixture was extracted with ethyl acetate, dried over sodium sulfate, and spin-dried under reduced pressure. The resultant is separated and purified by a silica gel column using a mixture of methanol and dichloromethane with a volume ratio of 1 / 198 to 2 / 98 as a developing solvent to obtain bisphenol A intermediate product a1. Dissolve 1.0-2.5g of a1 in 3-7mL of methanol, and then add it to 1-5mL of 3-5mol / L sodium hydroxide aqueous solution. Stir at 20-30°C for 8-16 hours. After the reaction mixture was washed with ethyl acetate, the aqueous phase was acidified to pH 2-4 with 28-35% HCl. The acidified aqueous phase was then extracted with ethyl acetate. Next, the organic phases were combined, d...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides an electrochemical immunoassay method for bisphenol A detection. The method comprises the steps of 1, competing reaction, wherein 5-20 microlitres of iso-volume mixed gradient dilution type solution of a sample to be detected or sample extraction solution and enzyme-labelled antigen solution diluted by phosphate buffered solution are added onto the surface of an enclosed electrode dropwise, the enclosed electrode is placed at 20-35 DEG C for incubation lasting 0.5-1.5 h, and flushing and blow-drying are conducted; 2, reduction peak current value detection, wherein the flushed electrode obtained from step 1 is immersed in 2-6 millilitre of ferrocenylmethanol solution of 0.2-1.5 mM, 20-60 microlitres of hydrogen peroxide of 5-15 mM is added at the same time, and then reduction peak current value is detected with the cyclic voltammetry method with a saturated calomel electrode as the reference electrode and a platinum electrode as the counter electrode. The immunoassay method is used for detection. The method overcomes the defect of a traditional enzyme-linked immunoassay method that detection is time-consuming, and reduces detection time.

Description

technical field [0001] The invention belongs to the technical field of immunochemistry and residue analysis of small molecule compounds, and in particular relates to an electrochemical immunoassay method for bisphenol A detection. Background technique [0002] Bisphenol-A is an endocrine disruptor derived from industrial chemicals. As an important organic chemical raw material, bisphenol A is mainly used in the production of various polymer materials such as polycarbonate and epoxy resin, and fine chemical products such as plasticizers, flame retardants, and coatings. From mineral water bottles, medical equipment to food packaging, most of them contain bisphenol A. However, because the surface of its finished product is easily degraded under the action of strong detergents, acidic or high-temperature liquids, there are residues in environmental water bodies, drinking water, aquatic products, vegetables, fruits and canned foods. Toxicology experiments have shown that bisphe...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N33/53G01N27/48
CPCG01N27/48G01N33/53
Inventor 陆旸丁敏玲李梦娟张燕刘国珍李翔王硕
Owner TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com