31 results about "Endocrine disruptor" patented technology

The invention discloses a fractionation method of an endocrine disrupter in an environmental water body, and belongs to the technical field of water body pollutant analysis. The fractionation method of the endocrine disrupter in the environmental water body comprises the following steps: S1, enriching and concentrating a water sample to obtain a to-be-fractionated liquid; s2, fractionating the to-be-fractionated liquid by adopting an analytical liquid chromatography system and a C18 semi-preparative chromatographic column to obtain fractions. According to the method, high-resolution synchronous fractionation of the endocrine disrupter in a wide polarity range (Log Kw:-0.19-6.62) is realized, the defects of low fractionation efficiency and high solvent consumption of the traditional semi-preparative liquid chromatography are effectively overcome by optimizing a gradient program of an analytical liquid chromatography system, the time and economic cost are saved, and the method is suitable for industrial production. And the fraction can be directly compatible with biological activity test after salting-out-extraction treatment.

The application discloses a preparation method of trace 10-component phenolic endocrine disruptor solution standard substance, which comprises the following steps: accurately weighing 10 kinds of high-purity phenolic compounds with accurate values, and dissolving the phenolic compounds in a mass spectrometry grade methanol solvent in a nitrogen atmosphere operation box to obtain a dissolved phenolic standard; filling nitrogen into a volumetric flask, pouring the dissolved phenolic standard into the volumetric flask, and dissolving and constant volume of the dissolved phenolic standard in a constant-temperature tank at 20.0 DEG C + / - 0.5 DEG C for 20 min, and then using the mass spectrometry grade methanol solvent to dissolve and constant volume to obtain a constant-volume phenolic solution; gradually diluting the constant-volume phenolic solution to 1 ug / L by using the mass spectrometry grade methanol solvent to obtain a diluted phenolic solution; pre-filling argon into a brown glass ampoule in a nitrogen atmosphere operation box, and then pouring the diluted phenolic solution into the brown glass ampoule, filling argon above the ampoule containing the solution, and fusion sealing the ampoule for refrigeration preservation.

The invention provides a bisphenol A induced rat benign prostatic hyperplasia model and a construction method, and relates to the technical field of animal models. The method comprises the following steps: S1, selecting rats which are 8-9 months old and 9-10 weeks old, and adaptively feeding the rats in a suitable environment for 7 days or more; s2, jointly applying high-fat feed and bisphenol A to the adaptively fed rats to induce benign prostatic hyperplasia; the induction time is 60-80 days, and performing biological index evaluation after the induction is completed. The exposure level of low-dose BPA adopted by the invention is closer to the actual environment exposure level of people, and a main exposure way is simulated through oral gavage. The model constructed by the method can better simulate the real process of chronic and low-dose exposure pathopoiesis of the environmental endocrine disrupter in etiology, and provides an experimental tool with more practical significance for related risk evaluation.

The utility model discloses an environmental endocrine disrupter content detection device which comprises a base, a stand column is arranged on the top face of the base, a supporting plate is fixedly arranged at the end, away from the base, of the stand column, and the environmental endocrine disrupter content detection device further comprises a detection mechanism and a control mechanism, a plurality of positioning cylinders are arranged on the top surface of the positioning plate, and detection cylinders for detecting environmental endocrine disruptors of liquid medicines with different contents are placed in the positioning cylinders; the temporary storage mechanism is fixedly arranged above the supporting plate, the temporary storage mechanism comprises a temporary storage box for storing an environmental endocrine disrupter solution, one side of the temporary storage box fixedly communicates with a connecting hose, the end, away from the temporary storage box, of the connecting hose fixedly communicates with a hollow cylinder, and a liquid dropping head is integrally arranged at the liquid outlet end of the hollow cylinder. The environmental endocrine disrupter content detection device is convenient for detecting the environmental endocrine disrupter content, and is accurate in detection result and high in working efficiency.

This application discloses a method for predicting the quantitative activity of endocrine disruptors, relating to the field of virtual screening of endocrine disruptors. The method includes: acquiring in vitro experimental data of nuclear receptors and removing duplicate data, as well as removing compound sets that do not contain the simplified molecular linear input canonical (SMILES) representation; using a molecular fingerprinting method to extract the primary, secondary, and tertiary structural features of the compounds. For each compound cluster, a quantitative prediction model based on machine learning or quantitative read-across is constructed to predict the quantitative activity value of the compound. Addressing the low efficiency of existing methods for predicting the quantitative activity of endocrine disruptors, this application extracts multi-level structural features of the compounds and constructs corresponding quantitative prediction models for compound structural clusters of different sizes. Through molecular docking and molecular dynamics simulations, the interaction mechanism between endocrine disruptors and nuclear receptors is studied from a structural biology perspective, thus improving efficiency.

The application discloses a method for predicting endocrine disruptors based on a harmful outcome path network, and belongs to the endocrine field, and comprises the following steps: acquiring experimental activity data containing qualitative data and quantitative data according to a pre-constructed harmful outcome path network; acquiring endocrine disruptor list data as a second data set; constructing a target qualitative prediction model according to the qualitative data; predicting target activity data of the second data set by using the target qualitative prediction model to obtain a target activity data set; constructing an endocrine disruptor effect qualitative prediction model by using the target activity data set; constructing a target quantitative prediction model according to the quantitative data; predicting whether a to-be-tested compound has an endocrine disruptor effect by using the endocrine disruptor effect qualitative prediction model; and predicting the corresponding target quantitative activity of the compound with the endocrine disruptor effect by using the target quantitative prediction model to obtain a quantitative harmful outcome path. The application realizes qualitative identification and quantitative classification of endocrine disruptors EDCs.

This application discloses a method, apparatus, medium, and device for identifying endocrine disruptors based on graph neural networks, relating to the field of biocommunication technology. Its main objective is to address the problem of low identification efficiency in existing endocrine disruptor technologies. The method includes: acquiring secondary mass spectrometry data of the organ to be tested; constructing a graph structure representation of the secondary mass spectrometry data, and predicting the graph structure representation based on a pre-trained disruptor prediction model to obtain predicted disruptor values. The disruptor prediction model is constructed based on a graph neural network, and the training sample dataset for the disruptor prediction model includes corresponding graph structure samples generated based on secondary mass spectrometry samples and interference effect information; generating an endocrine disruptor identification result based on a comparison between the predicted disruptor values ​​and a classification threshold, wherein the classification threshold is obtained based on classification evaluation indicators.

The invention discloses a method for constructing a social anomaly mouse model, relates to the technical field of animal model construction, and constructs a social anomaly model of a filial generation mouse by performing bisphenol S exposure on a female mouse in a pregnancy and nursing period. According to the mouse model constructed by the invention, in the pregnancy and nursing period, BPS is exposed, and the development of left secondary motor cortex neurons is damaged, so that progeny is induced to have social behavior disorder; the activity of neurons in the left M2 brain region is activated, so that social behavior disorders can be effectively relieved. The invention not only clarifies the neural development mechanism of the environmental endocrine disrupter-induced autism-like social disorder, but also provides a new thought of activating left M2 neurons as a potential intervention means, and can be used for developing drugs for treating the social behavior disorder induced by the environmental endocrine disrupter; the invention provides a new direction for etiological research and treatment strategy exploration of neurodevelopment disorder diseases taking social defects as core symptoms, and is beneficial to promoting deep development of related medical research.

The present application aims to provide a thermal recording material which is low in safety concerns of endocrine disruptors, excellent in color development and light resistance, and excellent in heat resistance. The thermal recording material (1) has a configuration in which a thermal recording layer (3) is layered on a substrate (2). The thermal recording layer (3) contains a color developer, a non-phenol color former, and a non-phenol ultraviolet absorber. The non-phenol ultraviolet absorber contains an oxanilide ultraviolet absorber. The non-phenol color former preferably contains a compound represented by the following formula (1) and / or a compound represented by the following formula (2). (The definitions of the symbols in the following formulae (1) and (2) are as described in the specification).

The application relates to the technical field of hydrogel preparations, and discloses a hydrogel preparation, a preparation method and application in repairing sperm damage. The method comprises the following steps: step one, constructing a recombinant plasmid; step two, plasmid transformation; step three, protein purification; step four, preparing mEXOs wrapped SKAP2 proteins; and step five, preparing a SKAP2 hydrogel preparation. In the application, SKAP2 proteins are expressed by means of a prokaryote Escherichia coli, milk exosomes are extracted, the SKAP2 proteins are then wrapped into the milk exosomes, and the exosomes containing the SKAP2 proteins are then fused into a hydrogel preparation. The sperm activity of asthenospermia mice can be significantly improved through skin smearing, including improving the sperm activity reduction effect of asthenospermia caused by heavy metal lead, organic endocrine disruptors phthalate, high temperature and aging, and the problem that there is no effective repair preparation for asthenospermia in the prior art is solved.

The invention discloses an endocrine disrupter identification method and system, and the method comprises the steps: carrying out the multi-dimensional feature extraction of a to-be-identified target compound, and obtaining the features of the target compound; respectively inputting the target compound characteristics into an activity prediction model corresponding to each prediction end point type to obtain an activity probability prediction result corresponding to each prediction end point; generating a recognition result of the target compound corresponding to each activity probability prediction result; according to the endocrine disrupter identification method, the identification efficiency of the endocrine disrupter is greatly improved.

The invention provides a detection method for simultaneously detecting multiple endocrine disrupters, which comprises the following steps: grinding tissue, homogenizing, adding an internal standard, extracting with three solvents: dichloromethane and methyl tert-butyl ether, an ethyl acetate-normal hexane mixed solution, acetonitrile and sodium chloride, combining and collecting the solutions to obtain a comprehensive extracting solution, concentrating and dissolving, degreasing, and drying to obtain the endocrine disrupters. Finally, a liquid chromatograph-mass spectrometer is used for detection, and efficient extraction and accurate analysis of the multiple endocrine disruptors in the tissue are achieved. According to the detection method, multiple organic solvents with different properties are adopted for extraction, detection of multiple endocrine disrupters can be synchronously achieved, the concentration level of the endocrine disrupters in the tissue sample can be directly measured, freeze drying treatment is not needed, operation is easy, and sensitivity and recovery rate are high.

The present application relates to wastewater treatment technical field, specifically to a kind of method for improving endocrine disruptor removal in wastewater by strengthening intermediate species adsorption, it includes the following steps: providing Ce-Co3S 4‑x Nano catalyst and peroxymonosulfate;Ce-Co3S 4‑x Nano catalyst and peroxymonosulfate are added to the wastewater containing endocrine disruptor to be treated, and Fenton-like catalytic reaction is carried out under stirring condition, and endocrine disruptor in wastewater to be treated is degraded.The method can efficiently and stably remove endocrine disruptor in wastewater, and can achieve almost 99.9% removal rate of bisphenol A within 9min, and 99.6% removal rate of pentachloronitrobenzene within 12min.

The invention discloses a preparation method of a carbon-modified vanadium disulfide nano material for oxidative removal of endocrine disrupters in a water body and application of the carbon-modified vanadium disulfide nano material to activation of persulfate. The preparation method comprises the following steps: firstly, using ammonium metavanadate as a vanadium source, thioacetamide as a sulfur source and L-ascorbic acid as a carbon source, and adjusting the pH value by using ammonia water; the molar ratio of ammonium metavanadate to thioacetamide to L-ascorbic acid is 1: 5: (1-5), and 2-4 mL of ammonia water is prepared into a precursor; the precursor is subjected to water bath ultrasonic treatment and then subjected to a hydrothermal reaction, washing and vacuum drying are conducted after the reaction is completed, and then the powdery carbon-modified vanadium disulfide is obtained. Compared with a single vanadium disulfide metal 1T phase, the carbon-modified vanadium disulfide nano material for oxidative removal of endocrine disrupters in a water body, prepared by the preparation method disclosed by the invention, is higher in composition, richer in microporous structure, higher in persulfate catalytic activation capability and reusability and lower in toxicity; under the same reaction condition, the degradation reaction rate constant is 4-79 times higher than that of common metal sulfide.

The present application relates to the technical field of detection, in particular to a method for detecting the exposure concentration of environmental endocrine disruptors in human body, comprising obtaining sample data, calculating sample concentration verification index; generating sample concentration step state signal according to sample concentration verification index; obtaining temperature data, calculating sample temperature evaluation index; generating sample temperature state signal according to sample temperature evaluation index; obtaining sample temperature data and sample refractive index data, calculating temperature-refraction evaluation index; generating temperature-refraction correlation signal according to temperature-refraction evaluation index; calculating stirring speed adjustment value according to temperature difference degree value; the present application analyzes the refractive index of sample and the temperature in the sample concentration process, adjusts the stirring speed in the sample concentration process, reduces the influence of sample processing process on the detection result, thereby improving the accuracy of the detection result of the exposure concentration of environmental endocrine disruptors in human body.

The invention relates to the technical field of biological monitoring, in particular to a fluorescence sensor for detecting endocrine disruption activity in sewage and application. A Y-type polypeptide containing an anchoring region, a hinge region and an antifouling region is creatively designed as a structural skeleton, and the tail ends of a main chain and a branch chain are respectively subjected to directional modification to prepare the multifunctional ligand-polypeptide-dye conjugate; an optical biosensing chip, namely a ligand-polypeptide-dye conjugate functionalized biosensing chip which is high in reaction efficiency, strong in anti-pollution characteristic and capable of being stably regenerated, is constructed by adopting a covalent coupling method, and then the quantum dot-nuclear receptor fluorescent probe which is high in sensitivity and adjustable in emission spectrum is prepared. The ratio fluorescence biosensing principle based on fluorescence resonance energy transfer is provided, rapid quantitative detection of the endocrine disruptors in sewage is achieved, and the endocrine disruptors have broad-spectrum specific recognition capacity on a certain type of endocrine disruptors.

The invention relates to the technical field of environmental endocrine disruptors, and discloses a biomarker for detecting EDC-induced glutamic acid synaptic dysfunction and a detection method thereof. The biomarker combination comprises molecules selected from at least one group of the following: presynaptic end function related proteins: plasma or cerebrospinal fluid (CSF) exosome levels including but not limited to synapsin-1 and vesicular glutamate transporter 1 (VGLUT1); proteins related to post-synaptic density and NMDA receptor functions include, but are not limited to, post-synaptic density protein 95 (PSD95), the phosphorylation level of a GluN1 subunit or soluble fragments thereof in CSF; the invention relates to astrocyte-neuron coupling related molecules. According to the invention, a multi-dimensional biomarker combination containing a presynaptic terminal function, a post-synaptic density and receptor function, astrocyte-neuron coupling and endoplasmic reticulum stress and calcium homeostasis is constructed, so that systemic evaluation of EDC-induced glutamic acid synaptic dysfunction is realized.

The invention relates to the field of environmental exposure omics and metabolic diseases, in particular to application of chlorpyrifos and paraquat in preparation of an abdominal obesity diagnosis product. The abdominal fat area, the A / S ratio, the liver fat content and other indexes measured through quantitative computed tomography (qCT) are combined, accurate recognition of abdominal obesity is achieved, the subjects with the normal body mass index (BMI) and visceral fat exceeding the standard can be effectively recognized, and the method has the advantages of being high in sensitivity, high in specificity and good in diagnosis foresight and has good application prospects. A new scheme is provided for early risk assessment and clinical screening of abdominal obesity, the significant correlation between the high exposure level of chlorpyrifos and paraquat and abdominal obesity supports the hypothesis that environmental endocrine disruptors promote obesity through metabolism interference, the urine concentration of chlorpyrifos and paraquat can be used as a novel biomarker, and the application prospect is broad. And a potential tool is provided for early screening of abdominal obesity.

The invention discloses a lake and reservoir endocrine disrupter concentration prediction and tracing method and system, and relates to the technical field of environment monitoring and pollution control, the method comprises the following steps: collecting and fusing multi-source heterogeneous monitoring data of a basin-lake and reservoir system, and constructing a standardized spatio-temporal data set; based on a hydrodynamic force and pollutant migration and transformation physical mechanism, key spatio-temporal features are extracted from the fusion data, and a high-dimensional feature tensor is constructed; constructing and training a deep neural network model fusing causal discovery and physical constraint, and predicting spatial and temporal distribution of the concentration of the endocrine disrupter in the lake and reservoir by using a feature tensor; analyzing the dynamic contribution degree of different pollution sources to the concentration of the endocrine disrupter in the target area to realize pollution source tracing; and carrying out integrated visualization on a concentration prediction result and pollution source contribution degree information, and generating graded early warning signals and targeted treatment decision suggestions. According to the method, the accuracy and robustness of lake and reservoir endocrine disrupter concentration prediction can be remarkably improved, and pollution traceability is realized.

The invention discloses a multi-dimensional topology analysis and dynamic energy coupling-based screening method for EDCs-induced HDP common hub targets. The method comprises the following steps: constructing common action targets of environmental endocrine disruptors (EDCs) and different subtypes (CH, GH, PE / EC) of gestational hypertension; the method comprises the following steps: constructing a PPI network, and innovatively screening common hub targets by adopting a maximum clique centrality (MCC) and degree centrality (Degree) dual topological algorithm; carrying out multi-dimensional passage enrichment analysis; and finally, constructing a molecular docking-molecular dynamics simulation cascade verification model, and carrying out dynamic conformation stability confirmation on the target spot. The invention systematically reveals a'co-disease mechanism 'that EDCs induce different HDP subtypes through targets such as STAT3, NFKB1, CTNNB1, CASP3 and BCL2 for the first time, effectively solves the problems of high false positive rate and lack of dynamic verification of an existing linear research mode, and provides a high-confidence scientific basis for developing a targeted broad-spectrum intervention strategy.

The invention belongs to the technical field of neurotoxicology and multi-omics detection, and particularly relates to an endocrine disrupter induced neurotoxicity biomarker screening and detection method which comprises the following steps: (1) establishing a neurotoxicity animal model; (2) verifying the neurotoxicity model; (3) performing brain tissue transcriptome sequencing and neural pathway analysis and / or performing brain tissue metabolome sequencing and differential metabolite analysis; (4) performing machine learning screening on the biomarkers; (5) carrying out interpretability analysis; (6) constructing a structural equation model; and (7) calculating a neurotoxicity comprehensive index NDTI, and carrying out significance detection and toxicity grade determination. By integrating behavior-neurotransmitter-hormone-inflammation-transcriptome / metabolome and other multi-level data, a reproducible neurotoxicity index is established, and graded quantitative evaluation of neurodevelopment damage caused by different endocrine disruptors and screening verification of key biomarkers are achieved.

The invention relates to the technical field of Raman scattering, in particular to a preparation method and application of an MXene (at) ZIF-67 (at) AgNPs composite type SERS (Surface Enhanced Raman Scattering) substrate, and the preparation method comprises the following steps: step 1, synthesizing a photoelectric double-gain type MXene (at) ZIF-67 heterojunction based on an in-situ solvothermal method, and step 2, loading AgNPs on MXene (at) ZIF-67 (at) AgNPs under the action of an amido bond to assemble the MXene (at) ZIF-67 (at) AgNPs composite type SERS substrate. And step 3, constructing an endocrine disrupter detection platform. According to the prepared MXene (at) ZIF-67 (at) AgNPs composite SERS substrate, an electromagnetic field is synergistically enhanced through a conductive network of MXene and a surface plasma resonance effect of AgNPs, meanwhile, an endocrine disrupter is enriched through a porous structure of ZIF-67, an SERS'hot spot 'is formed, and the Raman signal intensity and the detection sensitivity of the endocrine disrupter are greatly improved. The stability of the composite substrate can be improved by the inhibition effect of ZIF-67 on surface oxidation of the MXene sheet layer. Experiments show that a detection method based on the substrate is simple and easy to implement, and trace endocrine disrupters under a complex background can be effectively detected.

This invention discloses a method for detecting 99 achiral / chiral endocrine disruptors in urine based on a dual-column coupled analysis system, belonging to the field of pollutant monitoring and analysis technology. The method involves a single pretreatment of the urine sample, including formic acid-acetonitrile extraction and ethylenediamine-N-propyl silica gel purification. A dual-column coupled analysis mode is then used, with a C18 column simultaneously quantifying the total concentration of 93 achiral endocrine disruptors (EDCs) and 6 chiral pesticides. The chiral column yields the R / S enantiomer signal intensity ratio, and the content of each enantiomer is calculated using a "total-proportional conversion" model. This invention achieves, for the first time, a unified detection system for achiral and chiral substances, eliminating the need for a single enantiomer standard; accurate quantification can be achieved using only racemic mixtures, effectively avoiding the direct quantitative bias of chiral columns. The method has a limit of quantification as low as 0.25 μg / L, is simple to operate, low in cost, and has good reproducibility, providing an efficient and reliable analytical tool for population exposure monitoring and chiral selective metabolism research.

The application discloses a preparation method of a 21-component phenolic endocrine disruptor matrix standard substance in soil, which comprises the following steps: using high-standard farmland soil as an original matrix, performing acetone gradient extraction cleaning, ethanol gradient extraction cleaning and drying treatment on the original matrix soil to obtain clean matrix soil, and adding 21 kinds of phenolic compounds with accurate purity in a stepwise increasing manner to prepare the 21-component phenolic endocrine disruptor matrix standard substance in soil, wherein the corresponding concentration content can be accurately controlled in the range of 5 mg / kg to 10 mg / kg. The application provides a preparation process and a split process of the standard substance, the prepared standard substance is stable in composition, can be stored for 36 months under the conditions of light shielding and refrigeration, and the method is stable, reliable, and highly reproducible, and has a good popularization and application prospect.

The present application provides a model for predicting endocrine disruptors by integrating pharmacology and toxicology profiles, and a construction method and application thereof. Specifically, the present application constructs an endocrine disruptor prediction model based on pharmacology and toxicology data and in combination with molecular fingerprints of substructures of compounds. The endocrine disruptor prediction model of the present application can more accurately and efficiently evaluate whether a to-be-tested compound is an endocrine disruptor. The present application also develops an endocrine disruptor prediction system based on the endocrine disruptor prediction model.

The application discloses a method for constructing an endocrine simplified transcriptome based on a zebrafish embryo system and application thereof, and belongs to the field of high-throughput identification and screening of endocrine disruptors. By using the BORDA counting method, the evidence scores of three dimensions of gene centrality, information richness and redundancy degree are integrated, a specific endocrine interference simplified gene set is constructed, and the sequencing depth required for endocrine interference screening of each substance is greatly reduced, thereby reducing the test cost and improving the screening throughput of endocrine disruptors. The application provides help and support for rapid screening, mechanism analysis and harmful outcome prediction of new pollutants related to endocrine interference in China and the world.

The present application aims to provide a thermal recording material which is low in safety concerns of endocrine disruptors, excellent in color development and light resistance, and excellent in heat resistance. The thermal recording material (1) has a thermal recording layer (3) laminated on a substrate (2). The thermal recording layer (3) contains a color developer, a non-phenol color former, and a non-phenol light stabilizer. The non-phenol light stabilizer contains a histamine-based light stabilizer. The non-phenol color former preferably contains a compound represented by the following formula (1) and / or a compound represented by the following formula (2). (The definitions of the symbols in the following formulae (1) and (2) are as described in the specification).

The application relates to a method for treating endocrine disruptors in water by using layered hydrotalcite material and peracetic acid, belonging to the field of advanced oxidation technology and relating to a method for removing endocrine disruptors in water. The application aims to solve the problems of poor degradation efficiency, short service life of catalysts and high degradation cost in the prior art of using peracetic acid oxidant alone to degrade endocrine disruptors in water. The method comprises the following steps: I, preparing a metal salt solution; II, preparing a mixed solution of sodium hydroxide and sodium carbonate; III, preparing a gel; IV, aging; and V, adding layered hydrotalcite material and peracetic acid into wastewater containing endocrine disruptors to degrade the endocrine disruptors. The degradation rate of the endocrine disruptors in water can reach 100% within 30 minutes. The application is suitable for degrading endocrine disruptors.

The present application relates to the technical field of biological monitoring, in particular to a fluorescence sensor for detecting endocrine disrupting activity in sewage and application. The present application creatively designs a Y-shaped polypeptide containing an anchoring region, a hinge region and an anti-fouling region as a structural framework, and performs directional modification at the ends of the main chain and the branch chain to prepare a multifunctional ligand-polypeptide-dye conjugate; a covalent coupling method is used to construct a high-response-efficiency, strong-anti-pollution-characteristic and stable-regeneration optical biosensor chip, i.e. a ligand-polypeptide-dye conjugate functionalized biosensor chip, then a quantum dot-nuclear receptor fluorescence probe with high sensitivity and adjustable emission spectrum is prepared, a ratio fluorescence biosensing principle based on fluorescence resonance energy transfer is proposed, and rapid quantitative detection of endocrine disruptors in sewage is realized, so that the present application has broad-spectrum specific recognition ability for certain endocrine disruptors.