A construction method and application of SSR molecular fingerprint of "Shenyang" strawberry, screened 8 pairs of SSR primer sequences from 140 pairs of SSR primers covering the whole strawberry genome, and used them to establish SSR molecules of strawberry hybrid "Shenyang" Fingerprints can be used to identify the purity of strawberry hybrid "Shenyang" and its close relatives and sisters, non-close relatives and excellent lines from the genetic nature. It has high specificity, stable polymorphism, and band type combination. Easy to identify, good dominant characteristics, can accurately and quickly identify strawberry hybrids, and screen culture conditions, control the risk of somatic cell variation that tissue culture may bring, prevent vitrification, prevent callus formation, and limit tissue culture Multiplication algebra and multiplication coefficient, establish the micropropagation method of the strawberry hybrid "Shenyang", and quickly micropropagate the original strawberry seedlings of "Shenyang" with complete variety purity.