Construction method and application of SSR (Simple Sequence Repeat) molecular fingerprint map of strawberry hybrid

Abstract

The invention discloses a construction method and application of an SSR (Simple Sequence Repeat) molecular fingerprint map of strawberry hybrid. Eight pairs of SSR primer sequences are screened from 140 pairs of SSR primers which cover the whole genome of strawberries; the SSR molecular fingerprint map of the strawberry hybrid is constructed by utilizing the eight pairs of the SSR primer sequences; the purity of the seedlings of the strawberry hybrid Shenyang, a close relative sib line thereof, a non-near source variety, an excellent line and the like can be authenticated genetically; the SSRmolecular fingerprint map has the characteristics that the specificity is high, the polymorphism is stable, the band type combination is easy to identify and high in dominance, and can authenticate the strawberry hybrid accurately and quickly; moreover, culturing conditions are screened to control a somatic cell mutation risk which is possibly brought by tissue culture; the vitrification and the formation of calluses are stopped; the tissue culture proliferation generation number and the growth coefficient are inhibited; a micropropagation method of the strawberry hybrid Shenyang is determined; the Shenyang strawberry original seedlings with complete varietal purity can be micro-propagated quickly.

Description

technical field [0001] The invention relates to the field of construction of molecular fingerprints of fruit trees, in particular to a construction method and application of SSR molecular fingerprints of strawberry hybrids. Background technique [0002] Strawberry is a perennial plant of the Rosaceae Fragaria genus, an important horticultural economic crop, and ranks second in the world's berry production. Modern cultivated strawberries are allo-octoploid, highly heterozygous, and hybrid crops. Asexual reproduction is necessary to maintain the characteristics of the variety. However, long-term asexual reproduction in the field has buried hidden dangers for the production of strawberries caused by viral diseases. At the same time, it also brings Variety confusion in production. [0003] Strawberry anthracnose is a devastating fungal disease in strawberry seedlings. Through molecular marker-assisted screening, indoor inoculation of suspended spores of pathogenic bacteria, and...

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Problems solved by technology

[0006] In the prior art, although in sterile conditions and limited space, a large number of plant seedlings can be rapidly propagated through tissue culture, and strawberry tissue culture micropropagation seedling technology is also relatively common, but in production, tissue culture sources are often encountered. The problem of somatic cell variation of seedlings will cause many problems in production and application, so that many growers would rather use the field propagation route that is susceptible to viral diseases than take the risk of adopting tissue culture micropropagation seedlings. The reason is that somatic cell variation caused by inappropriate tissue culture and micropropagation process will lead to changes in various traits of plants, such as: flowering and fruiting time, fruit appearance, flavor, yield and other traits may change, among them, flowering delay, in production During this period, no flowers or fruit quality degradation is the most common phenomenon

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