4results about How to "Good polymorphism" patented technology

The invention belongs to the technical field of whole-genome gene chips, and particularly relates to a leymus chinensis whole-genome 50K liquid chip and application thereof. According to the leymus chinensis whole genome 50K liquid phase chip, a whole genome re-sequencing technology is utilized, an SNP molecular marker combination of leymus chinensis is determined, and then the leymus chinensis whole genome 50K liquid phase chip is developed. The chip is combined with a high-density SNP probe, realizes high-throughput and high-precision genetic typing of leymus chinensis genomes, and particularly has wide application prospects in the field of molecular breeding.

The invention provides a molecular marker related to soybean recurrent selection group physical property and application of the molecular marker, and relates to the technical field of biology, and the molecular marker is a 38759bp base sequence deleted in an interval of Chr13: 22776273bp to 222815032bp on a soybean MS1 gene. Molecular markers ms1A4 and ms1B1 and molecular markers ms1A6 and ms1B4 are designed and developed according to a soybean ms1 gene large fragment deletion region, and can be used for detecting genotypes of recurrent selection populations to determine fertility, and a new thought for screening specific genotypes or field phenotypes is provided. And the molecular markers of three genotypes of homozygous dominant (MS1MS1), heterozygous (MS1ms1) and homozygous recessive (ms1ms1) in a soybean ms1 recurrent selection group can be detected at one time. The technical problem that in the prior art, all genotypes of soybean recurrent selection groups cannot be detected at a time is solved.

This invention discloses SSR marker primers specific to the filamentous petals of gerberas and their applications. This invention utilizes SSR molecular markers to effectively identify different filamentous petal traits in gerberas, resulting in an SSR marker primer that can rapidly and accurately identify the petal traits of gerbera plants with different filamentous petals and the F1 generation of interspecific hybrids. The SSR marker primers provided by this invention can effectively distinguish between filamentous and non-filamentous petals in gerberas and exhibit significant polymorphism, with an accuracy greater than 65%. Furthermore, compared with traditional morphological trait observation methods, the identification method provided by this invention has better repeatability, saves time, and has high specificity. It can replace traditional morphological identification methods, effectively improving the molecular-level breeding process of gerberas and reducing the identification cycle of traditional morphological trait observation. It also allows for early screening to obtain gerbera offspring with superior filamentous varieties, which is of great significance for gerbera breeding.

The present invention is applicable to the field of biotechnology, and provides an SSR primer set developed based on transcriptome sequencing information of Kalanchoe strychnifolia and an application thereof in the identification of single-petal germplasm, comprising 20 pairs of SSR primer sets, wherein the nucleotide sequences of 1 to 20 pairs of primers are shown as SEQ ID No.1 to SEQ ID No.40. The present invention amplifies DNA of 48 Kalanchoe strychnifolia varieties and detects them by 6% non-denaturing polyacrylamide gel electrophoresis, and finds that the amplification products of 3 groups of SSR primers of the present invention have obvious band patterns and good polymorphism between varieties, and can quickly distinguish single-petal germplasm, thereby providing a reference for the identification, protection and genetic diversity analysis of Kalanchoe strychnifolia germplasm resources.