Obtaining method and application of molecular markers related to chrysanthemum drought resistance

Abstract

The invention belongs to the biotechnology field, provides an obtaining method of molecular markers related to chrysanthemum drought resistance, and is used for positioning and cloning of a chrysanthemum drought resistance excellent gene and breeding of new chrysanthemum varieties. The obtaining method comprises the steps: a, identification of the drought resistance; b, analysis of a chrysanthemum variety group structure; c, analysis of chrysanthemum variety genetic relationship; and d, determination of the molecular markers closely related to the chrysanthemum drought resistance. The method simultaneously adopts three molecular marker techniques comprising SRAP, SCoT and EST-SSR, the principles are different with each other and each have pertinency, and obtained dyeing strips are good in polymorphism and high in repeatability, can cover the entire genome, have rich number of strips and are beneficial for obtaining the molecular markers closely related to the drought resistance. 8 molecular markers related to the chrysanthemum drought resistance are totally obtained, early selection, directional selection and accurate selection of excellent drought-resistant varieties are achieved, the work load can be reduced, and the selection efficiency of chrysanthemum drought resistant genotypes is greatly improved, so as to speed up the process of breeding.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a method for obtaining molecular markers associated with drought resistance of chrysanthemums, which is used for the positioning and cloning of excellent drought resistance genes of chrysanthemums and the cultivation of new varieties of chrysanthemums. Background technique [0002] Chrysanthemum is one of my country's top ten traditional famous flowers and the world's four major cut flowers, and occupies an important position in flower production and landscaping. In recent years, there have been many genetic studies on ornamental traits such as chrysanthemum plant type and flower organ, which have promoted the process of chrysanthemum hybrid breeding to a certain extent. However, with the continuous deepening of breeding work, the traditional hybrid breeding method has brought certain blindness and limitations because it cannot improve specific traits. Molecular marker-assisted select...

AI Technical Summary

Problems solved by technology

However, the precise mapping and cloning of molecular markers associated with drought resistance in chrysanthemums have not yet been reported.

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