SSR molecular marker method for identifying foxtail millet variety and application

A technology of molecular markers and millet, which is applied in the direction of biochemical equipment and methods, microbial measurement/inspection, etc., can solve the problems of high cost, long cycle, and seasonal restrictions, and achieve better results and save time.

Active Publication Date: 2016-03-09
HEILONGJIANG BAYI AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although this method is simple and economical, it has a long cycle, high cost, and is limited by seasons, and the performance o

Method used

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  • SSR molecular marker method for identifying foxtail millet variety and application
  • SSR molecular marker method for identifying foxtail millet variety and application
  • SSR molecular marker method for identifying foxtail millet variety and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Embodiment 1: the establishment of method

[0026] 1. Prepare millet materials: wolf tail, white quicksand, dead car 1, Baiqinzhou yellow, Laolaibian, red sticky valley, knife handle, Gougenhong, Qianchuanzi, big green seedlings, yellow sticky valley, red millet 15 kinds of millet materials, namely , yellow grain, duck beak, big grain yellow, and red seedling valley, were planted in the greenhouse; millet sprouts about 10d-15d after germination were taken, frozen in liquid nitrogen, and stored in a -80°C ultra-low temperature refrigerator for later use; 15 The information of the millet varieties is shown in Table 1.

[0027] Table 115 Millet Variety Information

[0028] serial number

[0029] 2. Millet DNA extraction: Extract the DNA of various millet materials with a plant genome kit, and use 1% agarose gel electrophoresis to detect the DNA purity; dilute the DNA concentration of each material to 50ng / μL, and place it at -20 ℃ in the refrigerator for later ...

Embodiment 2

[0051] The present embodiment provides a method for identifying millet varieties using SSR molecular marker technology, comprising the following steps:

[0052] 1. Prepare millet materials: plant 45 kinds of millet materials such as Gogenhong, Longclaw, Xiaobaigu, Qianchuan, Knife Handle, Dalihuang, Baishagu, etc. in the greenhouse; take millet sprouts about 10d-12d after germination, and liquid After freezing with nitrogen, put them in a -80°C ultra-low temperature freezer and store them for later use; the information of the 45 millet varieties is shown in Table 7.

[0053] Table 745 Millet Variety Information

[0054]

[0055] 2. Millet DNA extraction: Extract the DNA of various millet materials with a plant genome kit, and use 1% agarose gel electrophoresis to detect the DNA purity; dilute the DNA concentration of each material to 50ng / μL, and place it at -20 ℃ in the refrigerator for later use;

[0056] 3. SSR primers: 5 pairs of SSR core primers suitable for the iden...

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Abstract

The invention discloses an SSR molecular marker method for identifying a foxtail millet variety and application, and belongs to the technical field of plant variety identification. The method comprises the steps that foxtail millet DNA is extracted, a PCR amplification reaction is performed through the foxtail millet DNA and 5 pairs of SSR core primers, and after electrophoresis detection is performed, the foxtail millet variety is identified according to an electrophoresis detection result; the nucleotide sequences of the SSR core primers are shown as SEQ ID NO.1-10. According to the method, the foxtail millet variety can be effectively distinguished, and an important way is supplied to further identification of a large number of the foxtail foxtail millet varieties.

Description

technical field [0001] The invention relates to an SSR molecular marker method and application for identifying millet varieties, belonging to the technical field of plant variety identification. Background technique [0002] Millet is a crop of the genus Setaria in the Gramineae family. It has physiological characteristics such as short growth period, drought tolerance, and barren tolerance. It is widely distributed in the temperate and tropical regions of Eurasia. It is one of the main cultivated crops in the middle and upper reaches of the Yellow River in China. At the same time, the nutritional value of millet is extremely high, rich in amino acids, proteins, vitamins and trace elements such as calcium, copper, iron, zinc, selenium, iodine, magnesium, etc., and its nutritional and health effects are also very significant. For many years, breed identification was based on morphological markers. Although this method is simple and economical, it has a long cycle, high cost,...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
CPCC12Q1/6858C12Q1/6895C12Q2600/156C12Q2531/113C12Q2565/125
Inventor 张东杰王颖沈琰杨义杰孙大庆赵雅楠
Owner HEILONGJIANG BAYI AGRICULTURAL UNIVERSITY
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