Molecular marker and screening method of gift strain nile tilapia oreochromis niloticus streptococcus iniae infection resistant family

A technology of Streptococcus iniae and Gifu tilapia, applied in biochemical equipment and methods, microbe measurement/testing, DNA/RNA fragments, etc., to achieve accurate identification and ensure stability

Inactive Publication Date: 2014-03-19
FRESHWATER FISHERIES RES CENT OF CHINESE ACAD OF FISHERY SCI
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Problems solved by technology

In the current breeding of parental lines of Gifu tilapia, it is impossible to distinguish whether the screened paren

Method used

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  • Molecular marker and screening method of gift strain nile tilapia oreochromis niloticus streptococcus iniae infection resistant family
  • Molecular marker and screening method of gift strain nile tilapia oreochromis niloticus streptococcus iniae infection resistant family
  • Molecular marker and screening method of gift strain nile tilapia oreochromis niloticus streptococcus iniae infection resistant family

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Embodiment Construction

[0028] The molecular marker method for screening jifu tilapia resistance to Streptococcus iniae infection families is carried out according to the following steps:

[0029] (1) Streptococcus dolphin infection: Select 120 excellent families of species preserved in the base, and select 25-30 tilapias for each family, and breed them in the same breeding environment for 2 months with the same specifications. Using 5×10 7 -3×10 8 The streptococcus iniae bacterial solution with CPU / cell concentration was used for artificial intraperitoneal injection infection, and the ratio of injection concentration to body weight was 600 μL / 100g. The cumulative mortality of each family after 48h was calculated. According to the normal distribution of different families after infection, 3 family individuals from the left and right extremes were selected as the sensitive group and the tolerant group.

[0030] Determination of infection concentration: select healthy general tilapia, artificially i...

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Abstract

The invention discloses a molecular marker and a screening method of gift strain nile tilapia oreochromis niloticus streptococcus iniae infection resistant family. The molecular marker is a sequence shown in SEQ ID NO.20; the screening method comprises five steps of streptococcus iniae infection, genome DNA extraction, primer screening, SRAP-PCR (Sequence-Related Amplified Polymorphism-Polymerase Chain Reaction) amplification and sequencing and analyzing, by the method, a parent family with stronger resistance to streptococcus iniae disease can be found more accurately and quickly, after enhanced cultivation, the parent family can be used as the parent for fingerlings propagation. The molecular marker screened out is prepared into a probe for detecting different parent families, the probe can sort out the streptococcus iniae disease resistant parent more simply and accurately, and selection of parent among different families is facilitated. Meanwhile, by using the bred disease-resistant parent as the propagation parent, quality of fingerlings can be improved further, and culture benefit of gift strain nile tilapia oreochromis niloticus can be increased.

Description

technical field [0001] The invention belongs to the selection and breeding technology of disease-resistant families in aquaculture, and particularly relates to molecular markers and screening methods of Gifu tilapia resistance to Streptococcus iniae infection families. Background technique [0002] Gifu tilapia has the advantages of fast growth, no intermuscular spines, rich amino acids and highly unsaturated fatty acids in muscles. Since it was introduced into China in 2006, it has been widely cultivated in southern provinces such as Guangdong, Guangxi, and Hainan, and has achieved good economic benefits. High-quality seed supply is the guarantee for the sustainable development of the tilapia industry. At present, the most important measure to improve seed quality is to artificially select high-quality parents. The method of artificial breeding is also relatively simple, based on some measurable morphological indicators, plus some specific genetic effect analysis. This k...

Claims

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Application Information

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IPC IPC(8): C12N15/11C12Q1/68
Inventor 强俊何杰徐跑朱志祥王辉董在杰
Owner FRESHWATER FISHERIES RES CENT OF CHINESE ACAD OF FISHERY SCI
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