67 results about "Streptococcus iniae" patented technology

The invention discloses high yield and high efficiency health culture feed of tilapia and a preparation method thereof. The feed comprises, by weight, 30 to 50 portions of protein powder, 10 to 18 portions of soybean meal, 6 to 12 portions of peanut meal, 8 to 14 portions corn, 4 to 7 portions of cottonseed meal, 5 to 8 portions of rapeseed meal, 10 to 16 portions of high-gluten flour, 1.8 to 3 portions of vitamins, 1 to 2 portions of amino acids, 1 to 1.8 portions of trace elements, 5 to 8 portions of vegetable oil, 1 to 2 portions of an attractant and 0.6 to 0.9 portions of a dolphin streptococcal biological preparation. The feed is prepared through drying, pulverizing, mixing and drying. The feed has a reasonable formula, is rich in nutrients, can increase the feed intake of tilapia suffering from streptococcal diseases, increases the growth rate, enhances the immune functions, reduces the death rate of tilapia suffering from streptococcal diseases and improves the cure rate of the streptococcal diseases.

The invention relates to the field of the molecular immunology, in particular to a streptococcus iniae vaccine and preparation and application thereof. Particularly, the vaccine is expressed by a base sequence in a sequence table SEQ ID No.1. A preparation method comprises the following steps: using streptococcus iniae G26 as a template and using a primer F2 / R5 to carry out PCR; and connecting a PCR product with plasmids pBTA1 and converting colon bacillus DH5l alpha by the connecting solution to obtain a bacterial strain DH5 alpha / pTASP11, i.e. a bacterial strain of the streptococcus iniae vaccine, which expresses a protein encoded by the base sequence in the sequence table SEQ ID No.1. The application of the streptococcus iniae vaccine is that the obtained bacterial strain of the streptococcus iniae vaccine is cultured by an LB culture medium until OD600 is 0.8 and then is dissolved in PBS and the obtained vaccine preparation solution has effect on immune protection for the streptococcus iniae. The obtained vaccine of the invention has very simple preparation process and does not need any extraction and purification process. The protection efficiency of the streptococcus iniae vaccine aiming at streptococcus iniae infection reaches 100 percent.

The invention provides a triple real-time fluorescent PCR method and a kit for detecting three streptococci at the same time. The kit comprises: a streptococcus agalactiae detection primer pair which is represented as the Seq ID No.1 and the Seq IN No.2 in the sequence table, a probe which is represented as the Seq ID No.3 in the sequence table, a streptococcus dysgalactiae detection primer pair which is represented as the Seq ID No.4 and the Seq ID No.5 in the sequence table, a probe which is represented as the Seq ID No.6 in the sequence table, a streptococcus iniae detection primer pair which is represented as the Seq ID No7 and the Seq ID No.8 in the sequence table, a probe which is represented as the Seq ID No.9 in the sequence table, three positive comparison sample which are represented as the Seq ID No.16, the Seq ID No.17 and the Seq ID No.18 in the sequence table, a negative comparison sample (ddH2O), a PCR buffering liquid required in fluorescent real-time quantification PCR amplification, dNTP and TaqDNAPolymerase. The method is simple and quick and is good in specificity.

The invention discloses a diagnostic kit for a Streptococcus iniae molecule and a detection method. The kit comprises lysis buffer solution A, DNA extraction B solution, DNA extraction C solution, DNA extraction D solution, positive template contrast E solution and PCR reaction solution F solution. The kit and the detection method consist of a pair of specific primers designed by a conserved sequence in a Streptococcus iniae gene group as a main body. Meanwhile, the invention optimizes the DNA extraction lysis solution and PCR reaction conditions, including magnesium ion concentration, annealing temperature and recurrent number. The kit can carry out qualitative detection on a specific DNA fragment of the Streptococcus iniae, is simple, convenient and rapid, has good specificity and high sensitivity, can be used for bacterium tracking detection of each cultivating process for fish cultivation, and has higher practical value.

The invention discloses a method for improving the streptococcus iniae infection resisting capability of GIFT tilapia in high-density culture. The method comprises the steps of preparing of compound Chinese herbal medicine, adding, feeding and breeding management. Compared with the prior art, the method has the following advantages: (1) the immunological protection of the GIFT tilapia in high-density culture can be effectively improved, and the survival rate of GIFT tilapia infected with streptococcus iniae is improved; (2) the compound Chinese herbal medicine added in a feed belongs to environment-friendly feed additives, and has less negative effect on fish and water; (3) the blood and head-kidney immunological stress response can be effectively enhanced, and the liver stress injury is reduced; (4) the adopted technological steps of the method are simple, the Chinese herbal medicine is low, and easy to obtain and preserve, and can be well absorbed and utilized by a fish body, and excrement of the GIFT tilapia does not impact the water quality.

The present disclosure relates to genetic markers for discrimination and detection of bacteria causing Edwardsiellosis and Streptococcosis in fish, and a method for discriminating and detecting the bacteria using the same. A genetic marker for discrimination and / or detection of each of Edwardsiella tarda, Streptococcus iniae, Streptococcus parauberis and Lactococcus garvieae, which cause fish diseases, is selected and a peptide nucleic acid and a primer pair, which are specific for the genetic marker, are used to amplify and obtain melting curves having different fluorescences depending on bacterial species. Thus, bacteria that cause fish diseases can be discriminated and whether or not fish would be infected with the bacteria can be detected in a simple, rapid and accurate manner.

The present invention relates to a field of vaccinology, and especially relates to a bacteria-delivered cross protection vaccine against Edwardsiella tarda and Streptococcus iniae. The cross protection vaccine is a recombinant strain obtained by plasmid [rho]DKS10 transforming escherichia coli DH5[alpha]. The vaccine obtained in the present invention has 83% and 75% protection efficiency respectively for Edwardsiella tarda and Streptococcus iniae infection.

The invention relates to a primer combination capable of detecting three kinds of streptococcus simultaneously. The primer combination consists of upstream and downstream primers capable of detectingstreptococcus agalactiae, as shown in SEQ ID MO:1, 2, upstream and downstream primers capable of detecting streptococcus dysgalactiae, as shown in SEQ ID MO:3, 4, and upstsream and downstream primerscapable of detecting streptococcus iniae, as shown in SEQ ID MO:5, 6. A reagent kit consists of a DNA extraction reagent and a PCR reaction reagent, wherein the DNA extraction reagent consists of a TEbuffer solution, proteinase k, chloroform, isopropyl alcohol, ethanol and redistilled water, and the PCR reaction reagent contains a reaction buffer solution, dNTPs, DNA polymerase, the 6 primers anddeionized water. A method for detecting the three kinds of streptococcus simultaneously comprises the steps of extracting DNA in samples through the extraction reagent, performing PCR amplification on the DNA through the reaction reagent, and performing gel electrophoresis on amplification products. The three kinds of streptococcus can be detected simultaneously, detection is simple, quick, highin sensitivity and high in specificity, and the primer combination and the method can meet requirements for large-scale molecular epidemiology investigation and analysis of aquatic animals and qualitysafety detection of aquatic products.

Isolated M proteins, or M-like proteins, of Streptococcus iniae, encoding nucleic acids, genetic constructs and antibodies which bind the isolated M proteins are provided. Also provided are isoforms and / or fragments of the M proteins, or M-like proteins, that display reduced fibrinogen binding. The isolated M proteins, antibodies and encoding nucleic acids maybe useful for diagnosis, immunization and / or therapy of Streptococcus iniae infections of animals such as fish and humans.

The invention relates to the molecular biology and immunology field, in particular to a gram positive microbes DNA vaccine and construction and application thereof. The vaccine has base sequence in sequence table SEQ ID No.1. The preparation method is that an eukaryotic expression vector pCN3 is used to construct streptococcus iniae DNA vaccine plasmid pCNS 10 which is suspended in PBS to be the DNA vaccine. The DNA vaccine has vaccine protection effect for streptococcus iniae and is the first construction in the whole world. The application is simple and once vaccine can achieve high efficiency and long-term vaccine protection effect.

The GapC plasmin binding protein genes of Streptococcus dysgalactiae (S. dysgalactiae), Streptococcus agalactiae (S. agalactiae), Streptococcus uberis (S. uberis), Streptococcus parauberis (S. parauberis), and Streptococcus iniae (S. iniae) are described, as well as the recombinant production of the GapC proteins therefrom. Also described is the use of the GapC proteins from those species in vaccine compositions to prevent or treat bacterial infections in general, and mastitis in particular.

The GapC plasmin binding protein genes of Streptococcus dysgalactiae (S. dysgalactiae), Streptococcus agalactiae (S. agalactiae), Streptococcus uberis (S. uberis), Streptococcus parauberis (S. parauberis), and Streptococcus iniae (S. iniae) are described, as well as the recombinant production of the GapC proteins therefrom. Also described is the use of the GapC proteins from those species in vaccine compositions to prevent or treat bacterial infections in general, and mastitis in particular.