23 results about "Streptococcus iniae" patented technology

The invention relates to the technical field of microorganisms, in particular to an antibacterial bacillus subtilis subsp. Deserticola strain and application thereof.Bacillus subtilis subsp. Deserticola D7-HSJ9 is obtained from mangrove forest rhizosphere soil by the applicant, and it is detected that the strain has good salt resistance, alkali resistance and antibacterial characteristics, and the bacillus subtilis subsp. Deserticola D7-HSJ9 can be used as a bacillus subtilis subsp. Deserticola strain. According to the present invention, the bacterial strain has characteristics of good antibacterial effect, particularly good antibacterial effect on streptococcus iniae, and good antibacterial effect on a plurality of pathogenic bacteria such as photobacterium dama subspecies, aeromonas salmonicida, staphylococcus aureus and the like, such that the bacterial strain can effectively replace the antibiotic so as to improve the prevention and control of fish diseases, and a good germplasm resource is provided for later preparation of microecological preparation products.

The invention relates to a multiplex PCR (polymerase chain reaction) primer group for simultaneously detecting four pathogens, a detection method and application, and belongs to the field of molecular biology, the four pathogens are nervous necrosis virus, iridovirus, streptococcus iniae and vibrio harveyi, and the primer group is composed of SEQ ID NO.1-8. By utilizing the primer, nervous necrosis virus, iridovirus, streptococcus iniae and vibrio harveyi can be simultaneously detected in a short time, and the aim of establishing a simple and effective method for simultaneously detecting various fish pathogens is fulfilled. And the detection primer group and the detection method provided by the invention have good specificity and relatively high sensitivity.

The invention relates to an SNP site associated with trachinotus ovatus and streptococcus iniae disease resistance characters and application, and belongs to the technical field of molecular biological breeding, the SNP site is located at the 14078454 site on the No.7 chromosome, the polymorphism site is C / T, and the genotype of the site with disease resistance is CC. The invention also provides application of the SNP site in identification of streptococcus iniae resistant trachinotus ovatus, and the application method comprises the following steps: extracting DNA (deoxyribonucleic acid) of the trachinotus ovatus to be identified, and selecting individuals with CC genotype at the SNP site through sequencing. And screening the genotype of which the SNP site is CC as a breeding parent. The method is used for cultivating a new trachinotus ovatus variety resistant to streptococcus iniae.

Methods of predicting survival following Streptococcus iniae infection in tilapia, the method comprising determining the alleles present at one or more, optionally two or more, DNA polymorphism in the tilapia and predicting whether or not the tilapia will survive Streptococcus iniae infection based on the determination of the alleles, methods of detecting in a sample from a tilapia the presence or absence of the alleles present at one or more, optionally two or more, DNA polymorphism associated with survival following Streptococcus iniae infection, relating methods of obtaining an indication of risk of a tilapia becoming infected by Streptococcus iniae comprising, methods of producing broodstock, offspring or eggs using so selected tilapia, all optionally using DNA polymorphisms located on linkage group 8 of the tilapia genome.

The invention relates to the technical field of new application of a natural orange peel extract, in particular to a bacteriostatic agent of the natural orange peel extract and application of the bacteriostatic agent. Bacteriostatic experiments find that the natural extract hesperetin and / or hesperidin of the orange peel has a good bacteriostatic effect on most of aquaculture indicator bacteria. Animal experiments after the hesperetin and / or hesperidin are prepared into the disease-resistant feed find that the hesperetin or hesperidin can effectively improve the resistance of fishes to streptococcus iniae infection. A 5 * MIC hesperetin group shows the best protection effect, the protection rate reaches 60%, and the protection rate of streptococcus iniae fed with a hesperidin group is 46%. Therefore, the hesperetin and / or the hesperidin which is the natural extract of the orange peel can be used as a bacteriostatic agent or a bacteriostatic feed in aquaculture, new application of the hesperetin and the hesperidin in aquaculture is developed, and the hesperetin and the hesperidin have important significance in controlling and preventing streptococcicosis.

The invention discloses a bacillus subtilis strain WN0101 and an application of the bacillus subtilis strain WN0101. The bacillus subtilis WN0101 is classified and named as bacillus subtilis, the bacillus subtilis WN0101 is preserved in the China Center for Type Culture Collection on September 29, 2025, and the preservation number of the bacillus subtilis WN0101 is CCTCC (China Center for Type Culture Collection) NO: M 20252145. The fermentation supernatant of the bacillus subtilis WN0101 can be used for specifically killing vibrio fluvialis and streptococcus iniae. In addition, the bacillus subtilis WN0101 has no hemolytic activity, and the fermentation supernatant of the bacillus subtilis WN0101 has good temperature stability and protease stability. Therefore, the bacillus subtilis WN0101 can replace antibiotics and is applied to prevention and treatment of vibrio fluvialis and streptococcus iniae infection.

The invention relates to the technical field of microorganisms, in particular to a fish streptococcicosis-resistant feed additive and application thereof.The strain D7-HSJ9 applied to the additive is obtained by screening of an applicant, it is detected that the strain has good salt resistance, alkali resistance and antibacterial characteristics, especially has a good antibacterial effect on streptococcus, and after processing, the strain can be used for preparing a feed additive for fish streptococcicosis. The feed additive prepared by compounding the strain and traditional Chinese medicines can well inhibit the growth of streptococcus iniae and streptococcus agalactiae, the additive is used for preparing disease-resistant feed, and the additive has the remarkable advantages of being scientific in component compatibility, obvious in synergistic effect, capable of meeting the growth requirement of fish, capable of remarkably promoting the growth of the fish, capable of improving the feed utilization rate and capable of reducing the feed conversion ratio. In addition, the disease-resistant feed also has the effects of effectively improving the immunity of the fish and reducing the morbidity of the fish, and is a biological feed additive and a biological feed which are low in cost and safe in color.

The application discloses a SNP molecular marker related to the resistance of yellowfin snapper to streptococcus iniae disease, which is characterized by being located at the 10398424th base of the 5th chromosome of the yellowfin snapper; the sequence of the SNP molecular marker is shown as SEQ ID NO:1, and the 251th base from the 5' end is G or A; the resistance of the individual with the GG or GA genotype to the streptococcus iniae disease is higher than that of the individual with the AA genotype. The application further discloses primers, a kit for detecting the SNP molecular marker, a method for breeding the yellowfin snapper with the resistance to the streptococcus iniae disease, and the application of the above-mentioned primers, the kit and the method in breeding the yellowfin snapper with the resistance to the streptococcus iniae disease.

The present invention relates to a multiplex PCR primer set for simultaneously detecting four pathogens, as well as a detection method and application thereof, belonging to the field of molecular biology. The four pathogens are neural necrosis virus, iridovirus, Streptococcus iniae, and Vibrio harveyi. The primer set comprises SEQ ID NOs. 1-8. Using the primers of the present invention, neural necrosis virus, iridovirus, Streptococcus iniae, and Vibrio harveyi can be simultaneously detected in a short period of time, thereby achieving the purpose of establishing a simple and effective method for simultaneously detecting multiple fish pathogens. Furthermore, the detection primer set and detection method of the present method have good specificity and high sensitivity.

The invention provides application of resveratrol in preparation of drugs or feed additives for preventing and treating fish streptococcus iniae diseases. The resveratrol has the remarkable activity of inhibiting the main virulence factor phosphoglucomutase (PGM) of streptococcus iniae, and the inhibition rate of the resveratrol to the activity of the PGM at the low concentration of 10 mu g / mL reaches 27.3%. A low-concentration natural compound is used as a virulence factor inhibitor to research and develop a medicine, is more economical, green and efficient, and is expected to be researched and developed into a natural green medicine for replacing antibiotics in aquaculture.

The invention discloses multiple qPCR (quantitative polymerase chain reaction) primers and a kit for detecting core pathogenic bacteria in aquaculture and application of the multiple qPCR primers and the kit. The multiple qPCR primer group comprises a primer combination A (SEQ ID NO.1-3) for detecting edwardsiella tarda, a primer combination B (SEQ ID NO.4-6) for detecting streptococcus iniae and a primer combination C (SEQ ID NO.7-9) for detecting aeromonas salmonicida. The invention also provides a kit containing the primer group and a detection method. A single-tube multiple detection system is constructed for three pathogenic bacteria which are most harmful in aquaculture, detection is rapid, and compared with a traditional culture method, the period is greatly shortened; the method is high in flux, low in cost and suitable for large-scale monitoring; and the kit has strong specificity and high sensitivity, and can realize early screening. The invention provides an effective tool for rapid, accurate and high-throughput detection of core pathogenic bacteria in aquaculture.

The invention discloses an SNP (Single Nucleotide Polymorphism) molecular marker related to streptococcus iniae disease resistance of sparus latus, which is characterized in that the SNP molecular marker is positioned at a 10398424-site basic group of a fifth chromosome of sparus latus; the sequence of the SNP molecular marker is as shown in SEQ ID NO: 1, and the basic group of the 251st site from the 5'end is G or A; wherein the streptococcus iniae disease resistance of a GG or GA genotype individual is higher than that of an AA genotype individual. The invention also discloses a primer and a kit for detecting the SNP molecular marker, and a method for breeding the sparus latus with streptococcus iniae disease resistance. The invention further discloses application of the primer, the kit and the method in breeding the streptococcus iniae disease resistant sparus latus.

The invention provides an antibacterial peptide ATMP23 with improved antibacterial activity, a mutant of the antibacterial peptide ATMP23 and application of the antibacterial peptide ATMP23 in preparation of an antibacterial agent. The amino acid sequence of the antibacterial peptide ATMP23 provided by the invention is shown as SEQ ID NO: 1, amino acid mutation and screening are carried out on the basis of the amino acid sequence, and antibacterial peptide mutants A23-M1, A23-M2 and A23-M3 with significantly improved antibacterial activity are obtained. The antibacterial peptide ATMP23 provided by the invention is small in molecular weight, convenient to artificially synthesize and low in cost, has a good bactericidal effect on vibrio splendidus, vibrio alginolyticus, vibrio parahaemolyticus, streptococcus agalactiae, streptococcus iniae, staphylococcus aureus, escherichia coli and the like, and can remarkably reduce the feed conversion ratio of livestock, poultry and aquatic products when being applied to livestock, poultry and aquatic feed; and the feed additive can improve the indexes of growth performance and immune function of bred animals, can be widely applied to the industries of feed, food, medicine and the like, and has a good market application prospect.

The invention relates to the technical field of feed additives, in particular to a traditional Chinese medicine composition for preventing and treating streptococcus iniae diseases of humpback perches as well as a preparation method and application of the traditional Chinese medicine composition. The traditional Chinese medicine composition comprises the following components in parts by weight: 10-15 parts of eucommia ulmoides, 10-15 parts of folium artemisiae argyi, 10 parts of radix angelicae, 10-13 parts of rhizoma zingiberis, 10-12 parts of liquorice, 7-9 parts of caulis spatholobi, 7-8 parts of herba ecliptae, 7-8 parts of radix ophiopogonis, 6-7 parts of poria cocos, 7-8 parts of hawthorn, 3-5 parts of radix aucklandiae, 3-4 parts of dendrobe, 3-5 parts of radix pulsatillae and 3-5 parts of radix puerariae. By feeding the traditional Chinese medicine composition provided by the invention, the weight gain rate of the humpback perches is increased by 20%, and the death rate of the humpback perches infected with streptococcus iniae is reduced by 10%.

The invention discloses a pelteobagrus fulvidraco calicivirus and streptococcus iniae dual fluorescent quantitative PCR method, and belongs to the technical field of biological diagnosis. The invention provides a pelteobagrus fulvidraco calicivirus and streptococcus iniae dual detection primer and a fluorescent probe. The pelteobagrus fulvidraco calicivirus and streptococcus iniae dual detection primer comprises primers as shown in SEQ ID NO.1-2 and SEQ ID NO.4-5, and the fluorescent probe comprises fluorescent probes as shown in SEQ ID NO.3 and SEQ ID NO.6. According to the present invention, the rapid and accurate diagnosis of the two pathogens is achieved by amplifying and detecting the two nucleic acids such as the pelteobagrus fulvidraco calicivirus and the streptococcus iniae by using the principle of the fluorescent quantitative PCR technology, and the lowest detection limits of the YcCV and the S. iniae are respectively 1 * 100 copies / [mu] L and 1 * 100 copies / [mu] L by using the detection method provided by the present invention. Important technical support is provided for prevention and control of diseases of the pelteobagrus fulvidraco.

The invention belongs to the field of livestock breeding or medicine, and particularly relates to application of a cinnamon leaf extract in the aspect of preventing and treating human-livestock diseases, fish streptococcus agalactiae diseases or dairy cow mastitis caused by streptococcus agalactiae. The cinnamon leaf extract has good bacteriostatic and bactericidal effects on streptococcus agalactiae and streptococcus iniae. Besides, the cinnamon leaf extract is strong in fragrance, green, environment-friendly, free of solvent residues and high in palatability, can be added into feed, has the effects of aroma enhancement and antibiosis, effectively reduces the use of antibiotics in fish breeding and dairy cow breeding processes, and is also beneficial to improving the food safety. In daily nursing prevention and treatment, the cinnamon leaf extract can also be used as an oil-soluble raw material to be used as an antibacterial agent for various florae such as streptococcus agalactiae so as to reduce the application of preservatives.

The invention discloses a tilapia streptococcus antigen protein and preparation and application of a segmented subunit nano-carrier vaccine of the tilapia streptococcus antigen protein, and belongs to the technical field of biological medicine. The invention discloses a preparation method of BNC-rEno (B-rE) and a segmented nano-carrier soaking vaccine (B-rE1 / B-rE2 / B-rE3) of the BNC-rEno (B-rE). The invention further discloses an immune protection effect of the subunit nano vaccine on tilapia mossambica to resist streptococcus infection. According to the Eno segmented subunit nano vaccine (B-rE3) disclosed by the invention, the serum antibody level of tilapia is remarkably improved, the death rate of tilapia infected with streptococcus agalactiae and streptococcus iniae is remarkably reduced, and the Eno segmented subunit nano vaccine has a relatively good immune protection effect on tilapia against streptococcus infection, can be used as a potential candidate vaccine for tilapia against streptococcus infection, and can be used for preparing the tilapia vaccine for preventing streptococcus infection. A new thought and an effective choice are provided for immune prevention and control of tilapia streptococcosis.

The invention discloses preparation and application of an Fc synergistic tilapia mossambica streptococcus bigeminy nanocellulose immersion vaccine, and belongs to the technical field of biology. The amino acid sequence of the recombinant protein rSSF is as shown in SEQ ID NO. 1. The invention also discloses a preparation method of a bigeminy subunit nano-carrier immersion vaccine prepared from the bigeminy recombinant protein. The invention also discloses an immune protection effect of the bivalent subunit vaccine on tilapia mossambica to resist streptococcus infection. According to the bivalent subunit vaccine disclosed by the invention, the serum antibody level of tilapia is remarkably improved, the death rate of tilapia infected with streptococcus agalactiae and streptococcus iniae is remarkably reduced, and the bivalent subunit vaccine has a relatively good immune protection effect on tilapia against streptococcus infection and can be used as a potential candidate vaccine for tilapia against streptococcus infection; a new thought and an effective choice are provided for immune prevention and control of tilapia streptococcosis.

The invention relates to the technical field of biology, in particular to recombinant lactic acid bacteria and application of the recombinant lactic acid bacteria in anti-streptococcus iniae disease vaccine.A research group recombines ENO genes of streptococcus iniae through a genetic engineering means, then converts the ENO genes into lactic acid bacteria and successfully expresses the ENO genes, the research group successfully expresses and purifies full-length ENO protein from the recombinant lactic acid bacteria, and the recombinant lactic acid bacteria have the advantages that the recombinant lactic acid bacteria can be used for resisting streptococcus iniae diseases; a challenge experiment verifies that the resistance to streptococcus iniae can be remarkably improved when fishes are fed with feed containing the recombinant lactic acid bacteria, related diseases caused by the streptococcus iniae can be effectively prevented, the immunity of the fishes can be improved, and the recombinant lactic acid bacteria are probiotics, have the disease-resistant and probiotic effects and are suitable for large-scale production. A new technical idea is provided for developing green biological feed, improving fish immunity and developing oral streptococcus iniae vaccines.

The invention relates to the technical field of microorganisms, in particular to a fish streptococcicosis-resistant fermented feed and a preparation method thereof.The bacillus subtilis subsp. Deserticola D7-HSJ9 applied to the fermented feed is obtained through self screening of an applicant, and it is detected that the strain has good salt resistance, alkali resistance and antibacterial characteristics, and the bacillus subtilis subsp. Deserticola D7-HSJ9 can be used for preparing the fish streptococcicosis-resistant fermented feed. Compared with a traditional feed processing technology, when the bacterial strain is applied to feed processing, the finished feed fermented by the technology can well retain bioactive components in the fermented feed; in the feed manufacturing process, high-temperature and high-pressure processes are omitted, energy is saved, the influence of high temperature and high pressure on probiotics is avoided, and the feed has the advantages of being large in number of viable bacteria, good in fermentation effect and the like; in addition, the product can also improve the immunity of the fish, reduce the morbidity of the fish and reduce the application of antibiotics in aquaculture, and is a green and safe fermented feed.

This invention discloses a triplet real-time PCR detection method for Streptococcus agalactiae, Streptococcus dolphinus, and Lactococcus gasseri. It utilizes three primer sets and probes: one for Streptococcus agalactiae, one for Streptococcus dolphinus, and one for Lactococcus gasseri. By optimizing the degradation temperature, primer dosage, and probe dosage, this invention effectively reduces the mutual interference of the three target pathogens, improving the accuracy of the detection results. This detection method has significant application value in fish farming. The reagent kit of this invention has low preparation cost, a simple preparation process, and is easily scalable for industrial production, thus possessing broad market prospects.

The invention provides an application of indole diterpenoid compounds in mangrove-derived fungus secondary metabolites in preparation of drugs for resisting aquaculture pathogenic bacteria. The indole diterpenoid compounds separated from fermentation metabolites of endophytic fungi Penicillium janthinellum D131 derived from mangrove kandelia candel rhizosphere have bacteriostasis effects on vibrio alginolyticus, vibrio frenesii, vibrio harveyi, streptococcus iniae and streptococcus agalactiae, so that the indole diterpenoid compounds can be used for preparing the medicines for resisting the harmful bacteria in aquaculture; the invention provides an important way for deeply excavating more valuable marine vibrio or streptococcus resistant new drugs used in the aquaculture process.

The invention belongs to the technical field of biology, and particularly relates to a macrobrachium rosenbergii MrBeclin1 gene, recombinant protein and application. The autophagy related gene MrBeclin1 is cloned and identified from macrobrachium rosenbergii, and the autophagy related gene MrBeclin1 encodes proteins of 426 amino acids and has a conservative APG6 structural domain. The gene has the highest expression in hemolymph, is positioned in cytoplasm, and has obviously up-regulated expression in hepatopancreas and gill after being stimulated by aeromonas hydrophila or LPS (Lipopolysaccharide). Functional mechanism research shows that MrBeclin1 interference can cause significant change of autophagy related gene expression and cause serious pathological damage to hepatopancreas and gill. Meanwhile, the recombinant protein can be directly combined with aeromonas hydrophila, citrobacter freundii, staphylococcus aureus and streptococcus iniae, and can specifically inhibit the growth of the first two gram-negative bacteria. The invention systematically clarifies the key effect of MrBeclin1 in immune defense of macrobrachium rosenbergii through dual mechanisms of autophagy regulation and direct bacteriostasis, and provides a new molecular target and theoretical basis for prevention and control of shrimp epidemic diseases.