10 results about "Streptococcus iniae" patented technology

The invention discloses a bacillus subtilis strain WN0101 and an application of the bacillus subtilis strain WN0101. The bacillus subtilis WN0101 is classified and named as bacillus subtilis, the bacillus subtilis WN0101 is preserved in the China Center for Type Culture Collection on September 29, 2025, and the preservation number of the bacillus subtilis WN0101 is CCTCC (China Center for Type Culture Collection) NO: M 20252145. The fermentation supernatant of the bacillus subtilis WN0101 can be used for specifically killing vibrio fluvialis and streptococcus iniae. In addition, the bacillus subtilis WN0101 has no hemolytic activity, and the fermentation supernatant of the bacillus subtilis WN0101 has good temperature stability and protease stability. Therefore, the bacillus subtilis WN0101 can replace antibiotics and is applied to prevention and treatment of vibrio fluvialis and streptococcus iniae infection.

The application discloses a SNP molecular marker related to the resistance of yellowfin snapper to streptococcus iniae disease, which is characterized by being located at the 10398424th base of the 5th chromosome of the yellowfin snapper; the sequence of the SNP molecular marker is shown as SEQ ID NO:1, and the 251th base from the 5' end is G or A; the resistance of the individual with the GG or GA genotype to the streptococcus iniae disease is higher than that of the individual with the AA genotype. The application further discloses primers, a kit for detecting the SNP molecular marker, a method for breeding the yellowfin snapper with the resistance to the streptococcus iniae disease, and the application of the above-mentioned primers, the kit and the method in breeding the yellowfin snapper with the resistance to the streptococcus iniae disease.

The invention discloses multiple qPCR (quantitative polymerase chain reaction) primers and a kit for detecting core pathogenic bacteria in aquaculture and application of the multiple qPCR primers and the kit. The multiple qPCR primer group comprises a primer combination A (SEQ ID NO.1-3) for detecting edwardsiella tarda, a primer combination B (SEQ ID NO.4-6) for detecting streptococcus iniae and a primer combination C (SEQ ID NO.7-9) for detecting aeromonas salmonicida. The invention also provides a kit containing the primer group and a detection method. A single-tube multiple detection system is constructed for three pathogenic bacteria which are most harmful in aquaculture, detection is rapid, and compared with a traditional culture method, the period is greatly shortened; the method is high in flux, low in cost and suitable for large-scale monitoring; and the kit has strong specificity and high sensitivity, and can realize early screening. The invention provides an effective tool for rapid, accurate and high-throughput detection of core pathogenic bacteria in aquaculture.

The invention discloses an SNP (Single Nucleotide Polymorphism) molecular marker related to streptococcus iniae disease resistance of sparus latus, which is characterized in that the SNP molecular marker is positioned at a 10398424-site basic group of a fifth chromosome of sparus latus; the sequence of the SNP molecular marker is as shown in SEQ ID NO: 1, and the basic group of the 251st site from the 5'end is G or A; wherein the streptococcus iniae disease resistance of a GG or GA genotype individual is higher than that of an AA genotype individual. The invention also discloses a primer and a kit for detecting the SNP molecular marker, and a method for breeding the sparus latus with streptococcus iniae disease resistance. The invention further discloses application of the primer, the kit and the method in breeding the streptococcus iniae disease resistant sparus latus.

The invention relates to the technical field of feed additives, in particular to a traditional Chinese medicine composition for preventing and treating streptococcus iniae diseases of humpback perches as well as a preparation method and application of the traditional Chinese medicine composition. The traditional Chinese medicine composition comprises the following components in parts by weight: 10-15 parts of eucommia ulmoides, 10-15 parts of folium artemisiae argyi, 10 parts of radix angelicae, 10-13 parts of rhizoma zingiberis, 10-12 parts of liquorice, 7-9 parts of caulis spatholobi, 7-8 parts of herba ecliptae, 7-8 parts of radix ophiopogonis, 6-7 parts of poria cocos, 7-8 parts of hawthorn, 3-5 parts of radix aucklandiae, 3-4 parts of dendrobe, 3-5 parts of radix pulsatillae and 3-5 parts of radix puerariae. By feeding the traditional Chinese medicine composition provided by the invention, the weight gain rate of the humpback perches is increased by 20%, and the death rate of the humpback perches infected with streptococcus iniae is reduced by 10%.

The invention discloses a pelteobagrus fulvidraco calicivirus and streptococcus iniae dual fluorescent quantitative PCR method, and belongs to the technical field of biological diagnosis. The invention provides a pelteobagrus fulvidraco calicivirus and streptococcus iniae dual detection primer and a fluorescent probe. The pelteobagrus fulvidraco calicivirus and streptococcus iniae dual detection primer comprises primers as shown in SEQ ID NO.1-2 and SEQ ID NO.4-5, and the fluorescent probe comprises fluorescent probes as shown in SEQ ID NO.3 and SEQ ID NO.6. According to the present invention, the rapid and accurate diagnosis of the two pathogens is achieved by amplifying and detecting the two nucleic acids such as the pelteobagrus fulvidraco calicivirus and the streptococcus iniae by using the principle of the fluorescent quantitative PCR technology, and the lowest detection limits of the YcCV and the S. iniae are respectively 1 * 100 copies / [mu] L and 1 * 100 copies / [mu] L by using the detection method provided by the present invention. Important technical support is provided for prevention and control of diseases of the pelteobagrus fulvidraco.

The invention relates to the technical field of biology, in particular to recombinant lactic acid bacteria and application of the recombinant lactic acid bacteria in anti-streptococcus iniae disease vaccine.A research group recombines ENO genes of streptococcus iniae through a genetic engineering means, then converts the ENO genes into lactic acid bacteria and successfully expresses the ENO genes, the research group successfully expresses and purifies full-length ENO protein from the recombinant lactic acid bacteria, and the recombinant lactic acid bacteria have the advantages that the recombinant lactic acid bacteria can be used for resisting streptococcus iniae diseases; a challenge experiment verifies that the resistance to streptococcus iniae can be remarkably improved when fishes are fed with feed containing the recombinant lactic acid bacteria, related diseases caused by the streptococcus iniae can be effectively prevented, the immunity of the fishes can be improved, and the recombinant lactic acid bacteria are probiotics, have the disease-resistant and probiotic effects and are suitable for large-scale production. A new technical idea is provided for developing green biological feed, improving fish immunity and developing oral streptococcus iniae vaccines.

This invention discloses a triplet real-time PCR detection method for Streptococcus agalactiae, Streptococcus dolphinus, and Lactococcus gasseri. It utilizes three primer sets and probes: one for Streptococcus agalactiae, one for Streptococcus dolphinus, and one for Lactococcus gasseri. By optimizing the degradation temperature, primer dosage, and probe dosage, this invention effectively reduces the mutual interference of the three target pathogens, improving the accuracy of the detection results. This detection method has significant application value in fish farming. The reagent kit of this invention has low preparation cost, a simple preparation process, and is easily scalable for industrial production, thus possessing broad market prospects.

The invention provides an application of indole diterpenoid compounds in mangrove-derived fungus secondary metabolites in preparation of drugs for resisting aquaculture pathogenic bacteria. The indole diterpenoid compounds separated from fermentation metabolites of endophytic fungi Penicillium janthinellum D131 derived from mangrove kandelia candel rhizosphere have bacteriostasis effects on vibrio alginolyticus, vibrio frenesii, vibrio harveyi, streptococcus iniae and streptococcus agalactiae, so that the indole diterpenoid compounds can be used for preparing the medicines for resisting the harmful bacteria in aquaculture; the invention provides an important way for deeply excavating more valuable marine vibrio or streptococcus resistant new drugs used in the aquaculture process.

The invention belongs to the technical field of biology, and particularly relates to a macrobrachium rosenbergii MrBeclin1 gene, recombinant protein and application. The autophagy related gene MrBeclin1 is cloned and identified from macrobrachium rosenbergii, and the autophagy related gene MrBeclin1 encodes proteins of 426 amino acids and has a conservative APG6 structural domain. The gene has the highest expression in hemolymph, is positioned in cytoplasm, and has obviously up-regulated expression in hepatopancreas and gill after being stimulated by aeromonas hydrophila or LPS (Lipopolysaccharide). Functional mechanism research shows that MrBeclin1 interference can cause significant change of autophagy related gene expression and cause serious pathological damage to hepatopancreas and gill. Meanwhile, the recombinant protein can be directly combined with aeromonas hydrophila, citrobacter freundii, staphylococcus aureus and streptococcus iniae, and can specifically inhibit the growth of the first two gram-negative bacteria. The invention systematically clarifies the key effect of MrBeclin1 in immune defense of macrobrachium rosenbergii through dual mechanisms of autophagy regulation and direct bacteriostasis, and provides a new molecular target and theoretical basis for prevention and control of shrimp epidemic diseases.