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Phage flocculant and application of phage flocculant in fermentation post-treatment technology

A processing technology and phage technology, applied in the direction of phage, virus/phage, microorganism, etc., can solve the problem of high power consumption cost of centrifuges and centrifugal aids, and achieve the effect of meeting the requirements of industrialized mass production, simple preparation process and cost reduction.

Pending Publication Date: 2019-04-09
PHAGELUX (NANJING) BIO-TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, when large-scale mass production is required, the cost of centrifuges, centrifugal auxiliary appliances, and power consumption in the above-mentioned post-fermentation treatment process is very high, and cost control is very important in industrial production, so it is urgent to provide a A flocculant that facilitates the separation of bacteriophage liquid and a post-fermentation treatment process applicable to industrial production

Method used

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  • Phage flocculant and application of phage flocculant in fermentation post-treatment technology
  • Phage flocculant and application of phage flocculant in fermentation post-treatment technology
  • Phage flocculant and application of phage flocculant in fermentation post-treatment technology

Examples

Experimental program
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Effect test

Embodiment 1

[0041] Flocculant sedimentation to obtain Vibrio parahaemolyticus phage process: Add 3% working concentration of polyferric aluminum chloride flocculant to the obtained mixed fermentation broth of Vibrio parahaemolyticus phage and Vibrio parahaemolyticus, manually or using a mixer (During mass production) Stir evenly, stand at room temperature for 3 hours, take the supernatant when upper and lower stratification occurs, and pass the supernatant through a 0.2 micron bacterial filter membrane to obtain a finished filtrate.

[0042] Centrifuge separation process to obtain Vibrio parahaemolyticus phage: centrifuge the obtained Vibrio parahaemolyticus phage and Vibrio parahaemolyticus mixed fermentation broth at 6000 rpm for 10 min, separate Vibrio parahaemolyticus and supernatant through a centrifuge, Take the supernatant and pass it through a 0.2 micron bacterial filter to obtain the finished filtrate.

[0043] Vibrio parahaemolyticus phage VP46, Vibrio parahaemolyticus phage VP4...

Embodiment 2

[0053] Flocculant sedimentation to obtain Xanthomonas rugosa phage process: Add 0.1% polysilicate flocculant at working concentration to the obtained mixed fermentation broth of Xanthomonas rugosa bacteriophage and Xanthomonas rugosa, manually or using a mixer (During mass production) Stir evenly, stand at room temperature for 3 hours, take the supernatant when upper and lower stratification occurs, and pass the supernatant through a 0.2 micron bacterial filter membrane to obtain a finished filtrate.

[0054] Centrifuge separation process to obtain Xanthomonas rugosa bacteriophage: Centrifuge the obtained Xanthomonas rugosa bacteriophage and Xanthomonas rugosa mixed fermentation broth at 6000 rpm for 10 minutes, pass Xanthomonas rugrugosa and supernatant through The centrifuge separates, and the supernatant is passed through a 0.2-micron bacterial filter to obtain a finished filtrate.

[0055] Get three groups of 500 ml of Xanthomonas rugosa phage preparations obtained in two ...

Embodiment 3

[0065] The process of obtaining Salmonella phage by flocculant sedimentation: Add 5% working concentration of polyphosphorus aluminum flocculant to the obtained Salmonella phage and Salmonella mixed fermentation broth, stir it manually or with a mixer (in mass production), and let it stand at room temperature for 3 hours. Take the supernatant during stratification, and pass the supernatant through a 0.2 micron bacterial filter to obtain the finished filtrate.

[0066] Centrifuge separation to obtain Salmonella phage process: centrifuge the obtained Salmonella phage and Salmonella mixed fermentation broth at 6000 rpm for 10 minutes, separate Salmonella and supernatant through a centrifuge, and take the supernatant to pass through a 0.2 micron bacterial filter membrane to obtain the finished filtrate.

[0067] Get three groups of 500 ml of Salmonella phage preparations obtained in two ways and let stand. The control examples containing flocculant and no flocculant were combined ...

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Abstract

The invention discloses a phage flocculant and application of the phage flocculant in a fermentation post-treatment technology and belongs to the field of a microbial post-treatment technology. The phage flocculant is characterized in that the phage flocculant is a cationic flocculant, and a working concentration of the phage flocculant is 0.1-5% generally. The flocculant is applied in the fermentation post-treatment technology of a vibrio parahaemolyticus phage, a coliphage, a salmonella phage, a xanthomonas axonopodis phage, a ralstonia solanacearum phage, a staphylococcus aureus phage, a streptococcus agalactiae phage, a streptococcus iniae phage or a pseudomonas aeruginosa phage. The phage flocculant can play a role of rapid subsidence of bacteria, be convenient and quick to operate, reduce centrifugal equipment investment and manpower and material resource loss and meet requirements of industrial mass production of the phage, and is safe, nontoxic and harmless to animals and plants.

Description

technical field [0001] The invention relates to the technical field of microbial post-treatment technology, more specifically, it relates to a bacteriophage flocculant and its application in post-fermentation treatment technology. Background technique [0002] Phage is a type of virus capable of infecting bacteria. It is small in size and simple in structure. It is mainly composed of protein capsid and nucleic acid wrapped inside. Bacteriophages are strictly parasitic, widely distributed in nature, can specifically lyse bacteria, have good safety, and have no toxic and side effects on the body and the environment. [0003] Phage is an important experimental tool and the most ideal material in the field of molecular biology. It can be used to prevent and treat diseases, identify bacterial types, screen and detect carcinogens, etc. It is a common process to increase the titer of phages by fermentation. [0004] The existing domestic patent with publication number CN102187937A...

Claims

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Application Information

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IPC IPC(8): C12N7/00C12N7/02
CPCC12N7/00C12N2795/00051
Inventor 乔欢徐旭凌陈海费文斌黄杰胡怿林何四龙丛郁肖逍丁良许文建樊小九王卫斌沈婵娟
Owner PHAGELUX (NANJING) BIO-TECH CO LTD
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