8 results about "Salmonella Phage" patented technology

The invention discloses a salmonella virulent bacteriophage vBSalPNW15 and application thereof, the bacteriophage is separated from farm sewage, belongs to a long-tail bacteriophage family, has a regular icosahedron head and a long-tail structure, and keeps stable under the conditions that the pH is 4-13 and the temperature is 40-60 DEG C. Genomic analysis shows that the kit does not contain virulence genes and drug-resistant genes and is good in safety. The bacteriophage and cinnamyl aldehyde are combined to be used for preventing and treating salmonella infection, both the bacteriophage and cinnamyl aldehyde show a remarkable synergistic bacteriostatic effect (FICI = 0.5) in vitro and in vivo, and the bacteriophage and cinnamyl aldehyde can effectively inhibit growth of multi-drug-resistant salmonella, remove biological membranes, relieve tissue pathological damage and remarkably improve the survival rate of chicks. The invention provides a safe and efficient antibiotic replacement scheme for preventing and treating salmonella infection, and is suitable for the fields of livestock and poultry breeding and food safety.

This invention discloses a novel virulent Salmonella phage vB_Sal_SM6 and its phage cocktail composition. Phage vB_Sal_SM6 exhibits lytic activity against multiple serotypes of Salmonella. A phage cocktail prepared by mixing phage vB_Sal_SM6 with phage vB_VpaP_G1 achieved a lysis rate of 98.11% against 53 Salmonella strains. In vivo experiments showed that this phage cocktail, administered via drinking water, significantly reduced the number of Salmonella in laying hen feces, achieving a bactericidal rate of 99.59%. It improved growth performance decline, liver function damage, egg quality decline, and intestinal mucosal damage caused by Salmonella infection, without adverse effects on healthy laying hens. The phage and its cocktail provided by this invention have a broad host spectrum, high safety, and significant antibacterial effect, making them suitable for the prevention and control of Salmonella infection in poultry and showing promising application prospects.

The invention discloses a salmonella bacteriophage, a bacteriophage conjugate and a preparation method and application of the salmonella bacteriophage and the bacteriophage conjugate, and relates to the technical field of biology, the salmonella bacteriophage is bacteriophage 72-1B, the preservation number is M20252570, and the preservation unit is China Center for Type Culture Collection. The preparation method comprises the following steps: specifically coupling a fluorescent material with a bacteriophage 72-1B to prepare a bacteriophage 72-1B-fluorescent material 9-(2-bromoethyl) carbazole conjugate; the phage conjugate combines the characteristics of a fluorescent material and the phage and can specifically recognize and combine salmonella, and the detection limit is as low as 10 < 2 > CFU / mL; the distribution positions (such as food pollution sites and wound infection areas) of bacteria can be directly observed through a fluorescence microscope and flow cytometry, so that visual positioning is realized, an intuitive basis is provided for pollution traceability and targeted therapy, and blind prevention and control are avoided.

The invention discloses a cocktail preparation based on duck-origin salmonella adaptive evolution bacteriophage and application of the cocktail preparation, and belongs to the technical field of biological medicine. The cocktail preparation consists of a phage phi ST 705 with the preservation number of CCTCC (China Center for Type Culture Collection) NO: M 2025836, a phage phi R2 with the preservation number of CCTCC NO: M 2025837 and a phage phi R1-1-1 with the preservation number of CCTCC NO: M 2025838. The bacteriophage phi ST 705 is separated and purified from sewage of a farm, and the host bacterium is salmonella ST 705; the bacteriophage phi R2 and the bacteriophage phi R1-1-1 correspond to bacteriophages separated from the salmonella ST 705 in an adaptive evolution strain under the continuous selection pressure of the bacteriophage phi ST 705. The cracking capacity and the host spectrum of the cocktail preparation are remarkably expanded, and the resistant mutation rate of salmonella ST 705 can be effectively reduced.

The present application relates to the technical field of Salmonella bacteriophage, and particularly relates to a bacteriophage capable of lysing Salmonella Blegdam (Salmonella Blegdam bacteriophage) and a composition and application thereof. The bacteriophage is Salmonella bacteriophage RDP-SA-22010, the preservation number of which is CGMCC No. 45274, and the bacteriophage was preserved in the China General Microbiological Culture Collection Center on September 21, 2022. The Salmonella bacteriophage RDP-SA-22010 can also lyse Salmonella enteritidis, Salmonella pullorum and Salmonella kentucky. A composition comprises the Salmonella bacteriophage RDP-SA-22010. A reagent or kit comprises the above-mentioned composition. The present application also provides that the Salmonella bacteriophage RDP-SA-22010 can be applied to the preparation of a biological sterilizing agent, the preparation of a medicine for treating diseases caused by Salmonella and the preparation of a feed additive. The Salmonella bacteriophage RDP-SA-22010 in the present application provides a new biological control means for the prevention and treatment of Salmonella Blegdam.

The invention provides double probes for detecting salmonella, a kit as well as a preparation method and application of the kit, and belongs to the technical field of bacterial detection. The double probes comprise a capture probe and a detection probe; the capture probe is a magnetic bead coupled with a salmonella aptamer and a salmonella bacteriophage, and the detection probe is gold nanoparticles modified with fDNA; the salmonella aptamer and the fDNA are of a single-chain structure with mutually paired sequences. The double probes provided by the invention respectively promote the sensitive and convenient development of a salmonella detection technology through efficient capture, separation and enrichment and visual signal output. The scheme provided by the invention is simple to operate, low in cost, less in time consumption, easy to implement, high in sensitivity and high in accuracy, does not need large experimental equipment, and can realize visualization of detection results.

The invention discloses a salmonella bacteriophage nano-emulsion preparation as well as a preparation method and application thereof. The salmonella bacteriophage nano-emulsion preparation is mainly prepared from the following active ingredients in percentage by mass: 5 to 15 percent of salmonella bacteriophage suspension, 84 to 94 percent of edible oil, 0.5 to 3.0 percent of Pickering stabilizer dispersion liquid and 0 to 2.0 percent of protective agent. The invention also discloses a preparation method of the salmonella bacteriophage nano-emulsion preparation and application of the salmonella bacteriophage nano-emulsion preparation in bacteriostasis and preservation of meat products. The salmonella bacteriophage nano-emulsion preparation disclosed by the invention is more essential in stable mechanism, more stable in system, more beneficial to bacteriophage activity maintenance, better in food applicability, safe, friendly, more durable in bacteriostatic action and more suitable for fresh keeping of meat products when being applied.

The application discloses a Salmonella bacteriophage and application thereof, the bacteriophage is Salmonella bacteriophage TZCZ65, is preserved in China typical culture preservation center, CCTCC NO: M 20242879, the preservation date is December 23, 2024, and the preservation address is Wuhan.The Salmonella bacteriophage TZCZ65 disclosed in the application has good stability, no virulence drug resistance gene, high safety, has lysis activity to Salmonella of different sources, wide host range, short latent period, high burst size of progeny bacteriophage, and can be applied to livestock and poultry slaughtering, processing and breeding links, to provide a safe and efficient biological antibacterial product for livestock and poultry and products thereof.