19 results about "Salmonella Phage" patented technology

The invention discloses a salmonella virulent bacteriophage vBSalPNW15 and application thereof, the bacteriophage is separated from farm sewage, belongs to a long-tail bacteriophage family, has a regular icosahedron head and a long-tail structure, and keeps stable under the conditions that the pH is 4-13 and the temperature is 40-60 DEG C. Genomic analysis shows that the kit does not contain virulence genes and drug-resistant genes and is good in safety. The bacteriophage and cinnamyl aldehyde are combined to be used for preventing and treating salmonella infection, both the bacteriophage and cinnamyl aldehyde show a remarkable synergistic bacteriostatic effect (FICI = 0.5) in vitro and in vivo, and the bacteriophage and cinnamyl aldehyde can effectively inhibit growth of multi-drug-resistant salmonella, remove biological membranes, relieve tissue pathological damage and remarkably improve the survival rate of chicks. The invention provides a safe and efficient antibiotic replacement scheme for preventing and treating salmonella infection, and is suitable for the fields of livestock and poultry breeding and food safety.

The utility model discloses a salmonella bacteriophage screening device, relates to the technical field of salmonella bacteriophage screening, and aims to solve the problems that when multi-thallus culture is carried out on existing culture dishes, most of the culture dishes are directly placed in one culture dish, mixing is easily caused, observation is influenced, and screening is inaccurate. A culture cavity is formed in the culture dish body, the culture cavity and the culture dish body are of an integrated structure, and a protective cover is arranged at the upper end of the culture dish body; the central column is arranged in the culture cavity, partition plates are arranged on the peripheral surfaces of the central column, and the partition plates and the central column are of an integrated structure; the limiting plates are arranged on the inner wall of the culture cavity, the limiting plates are connected with the inner wall of the culture cavity in a hot melting mode, limiting inserting grooves are formed in the limiting plates, the three faces of each limiting inserting groove are open, the partition plates are inserted into the limiting inserting grooves, and the number of the partition plates and the number of the limiting plates are both four.

The present invention provides a solid preparation of Salmonella phage, a preparation method thereof and an application thereof, belonging to the technical field of bioengineering. The solid preparation is a dry powder obtained by spray-drying a biologically active phage and a protective agent and then mixing with a carrier. The phage is selected from Salmonella phage S4P and Salmonella phage S16P. Among them, the preservation number of Salmonella phage S4P is CCTCC NO: M2021765, and the preservation number of Salmonella phage S16P is CCTCC NO: M2021861. The solid preparation of Salmonella phage of the present invention can be stored at room temperature for a long time, still has a high titer level after being stored at high temperature for a certain period of time, also has good bile salt tolerance, and the adopted preparation method has the advantages of low cost, short cycle, simple process, etc. compared with freeze-drying.

This invention discloses a novel virulent Salmonella phage vB_Sal_SM6 and its phage cocktail composition. Phage vB_Sal_SM6 exhibits lytic activity against multiple serotypes of Salmonella. A phage cocktail prepared by mixing phage vB_Sal_SM6 with phage vB_VpaP_G1 achieved a lysis rate of 98.11% against 53 Salmonella strains. In vivo experiments showed that this phage cocktail, administered via drinking water, significantly reduced the number of Salmonella in laying hen feces, achieving a bactericidal rate of 99.59%. It improved growth performance decline, liver function damage, egg quality decline, and intestinal mucosal damage caused by Salmonella infection, without adverse effects on healthy laying hens. The phage and its cocktail provided by this invention have a broad host spectrum, high safety, and significant antibacterial effect, making them suitable for the prevention and control of Salmonella infection in poultry and showing promising application prospects.

The invention discloses a salmonella bacteriophage, a bacteriophage conjugate and a preparation method and application of the salmonella bacteriophage and the bacteriophage conjugate, and relates to the technical field of biology, the salmonella bacteriophage is bacteriophage 72-1B, the preservation number is M20252570, and the preservation unit is China Center for Type Culture Collection. The preparation method comprises the following steps: specifically coupling a fluorescent material with a bacteriophage 72-1B to prepare a bacteriophage 72-1B-fluorescent material 9-(2-bromoethyl) carbazole conjugate; the phage conjugate combines the characteristics of a fluorescent material and the phage and can specifically recognize and combine salmonella, and the detection limit is as low as 10 < 2 > CFU / mL; the distribution positions (such as food pollution sites and wound infection areas) of bacteria can be directly observed through a fluorescence microscope and flow cytometry, so that visual positioning is realized, an intuitive basis is provided for pollution traceability and targeted therapy, and blind prevention and control are avoided.

The invention provides a freeze-drying protective agent and a penicillin bottle freeze-drying method of salmonella bacteriophage, and belongs to the technical field of biology. The freeze-drying protective agent comprises the following components in percentage by mass: 5%-10% of trehalose, 1%-3% of mannitol, 1%-3% of lactose, 1%-3% of sodium citrate, 0.5%-2% of linseed oil, 10%-20% of skim milk powder, 1%-3% of sodium sulfate and 1%-3% of succinic acid. According to the penicillin bottle freeze-drying method of the salmonella bacteriophage, the freeze-drying recovery rate of the salmonella bacteriophage can be remarkably improved; the freeze-dried bacteriophage preparation is small in size, light in weight, easy to transport and store and capable of keeping activity for a long time at normal temperature.

The present invention relates to the field of biological bacteriophage technology, and discloses a bacteriophage for lysing Salmonella and Escherichia coli, and the bacteriophage is named Salmonella phage GRNPK. The bacteriophage is a virulent bacteriophage, has a strong lysis effect on both Salmonella and Escherichia coli, and belongs to the Demerecviridae family; the bacteriophage forms small and transparent plaques on a double-layer agar plate; has high stability, is active under pH 2-12 and under conditions of -20°C-70°C; has strong infectivity, and is cultured for 12 hours under the condition of an optimal multiplicity of infection (MOI) of 0.001, and has a titer of 1.1×10 12 PFU / mL. High-throughput sequencing showed that the phage GRNPK did not encode any lysogeny, toxin, drug resistance and virulence factor-related genes. In vitro experiments showed that GRNPK could effectively inhibit the growth of Salmonella and Escherichia coli and delay the bacteria from developing resistance to the phage. Animal test results showed that the phage could effectively reduce the number of Enteritidis Salmonella in the intestines of chicks.

The invention belongs to the technical field of microbiology and agricultural application thereof, and particularly relates to a salmonella bacteriophage, a microbial preparation and application of the salmonella bacteriophage. The salmonella bacteriophage disclosed by the invention belongs to a long-tail bacteriophage, has relatively good killing and destroying capability on host bacteria drug-resistant salmonella typhimurium SB56, also can have different degrees of splitting action on other 16 clinical drug-resistant salmonella typhimurium, is relatively wide in antibacterial spectrum, and has the potential of being applied to drugs for preventing and treating salmonella typhimurium; the salmonella bacteriophage pSty.S56. XXXSZ disclosed by the invention is relatively short in latency period and relatively strong in environmental tolerance, and can achieve a synergistic effect with antibiotics, so that the antibacterial ability is relatively lasting, and the dosage of avidin is reduced.

The invention discloses a cocktail preparation based on duck-origin salmonella adaptive evolution bacteriophage and application of the cocktail preparation, and belongs to the technical field of biological medicine. The cocktail preparation consists of a phage phi ST 705 with the preservation number of CCTCC (China Center for Type Culture Collection) NO: M 2025836, a phage phi R2 with the preservation number of CCTCC NO: M 2025837 and a phage phi R1-1-1 with the preservation number of CCTCC NO: M 2025838. The bacteriophage phi ST 705 is separated and purified from sewage of a farm, and the host bacterium is salmonella ST 705; the bacteriophage phi R2 and the bacteriophage phi R1-1-1 correspond to bacteriophages separated from the salmonella ST 705 in an adaptive evolution strain under the continuous selection pressure of the bacteriophage phi ST 705. The cracking capacity and the host spectrum of the cocktail preparation are remarkably expanded, and the resistant mutation rate of salmonella ST 705 can be effectively reduced.

The invention belongs to the technical field of microbial sterilization preparations, and particularly discloses a broad-spectrum salmonella bacteriophage SD40-16 and application of the broad-spectrum salmonella bacteriophage SD40-16. The bacteriophage SD40-16 can be used for cracking six serotypes of salmonella and escherichia coli including salmonella enteritidis and salmonella typhimurium, is wide in host range and strong in killing capability, and can be used for inhibiting the growth of bacteria. And meanwhile, the strain has good thermal stability and acid-base tolerance, and is easy to proliferate and enrich. The bacteriophage can enhance the antibacterial ability of kanamycin, provides a new drug combination strategy, has an obvious synergistic effect on salmonella resistance when being combined with kanamycin, and has a good application prospect in the aspect of preventing and treating salmonella infection.

The invention discloses a salmonella bacteriophage and application thereof, the salmonella bacteriophage is salmonella bacteriophage TZCZ65 and is preserved in China Center for Type Culture Collection, CCTCC NO: M 20242879, the preservation date is December 23, 2024, and the preservation address is Wuhan. The salmonella bacteriophage TZCZ65 disclosed by the invention is good in stability, free of non-toxic drug-resistant genes, high in safety, wide in host range, short in incubation period and high in filial generation bacteriophage outbreak amount, has splitting activity on salmonella from different sources, can be applied to livestock and poultry slaughtering, processing and breeding links, and has broad application prospects. A safe and efficient biological antibacterial product is provided for livestock and poultry and products thereof.

The invention discloses a strain of Salmonella phage vB-SenS-JZ291 and its application, belonging to the field of biotechnology. The Salmonella phage vB-SenS-JZ291 can lyse different serotypes of Salmonella such as Typhimurium, Typhimurium 1,4,[5],12:i:-unidirectional variant, Enteritis, Pullorum, Thompson and Mbandaka, and the lysis rates of Enteritis serotype, Typhimurium 1,4,[5],12:i:-unidirectional variant and Typhimurium serotype Salmonella are 94.7%, 96.2% and 88.6% respectively. The phage can effectively remove the content of mixed contamination of different serotypes of drug-resistant Salmonella in multiple foods within 12 hours under the conditions of 4°C and 25°C. The invention also provides an effective combination of a phage and a preservative, and the removal rate of mixed contamination of multiple drug-resistant Salmonella in food reaches 96.8%.

The present invention belongs to the field of biotechnology, and particularly relates to a Salmonella phage SPYS_2 and its application. The present invention provides a Salmonella phage SPYS_2, which is deposited in the China Center for Type Culture Collection with the deposit number of CCTCC NO: M2024985. The Salmonella phage SPYS_2 of the present invention has strong tolerance to temperature and pH, adapts to various environments, proliferates rapidly, is suitable for large-scale production, and can effectively kill Salmonella, and can be used for the prevention or treatment of Salmonella infection in animal breeding.

The present invention discloses a Clostridium perfringens phage PM11, a phage composition thereof and an application thereof. The Clostridium perfringens phage PM11 for preventing and controlling poultry bacterial diarrhea has a preservation number of CGMCC No. 22372. The phage was deposited in the China General Microbiological Culture Collection Center on April 12, 2021. The present invention also discloses a phage composition formed by combining the Clostridium perfringens phage PM11 with Escherichia coli phage PD205 and Salmonella phage PC192. The above-mentioned Clostridium perfringens phage and phage composition can be used as an active ingredient for effectively preventing and treating poultry bacterial diarrhea, and can also be used as an environmental disinfectant to effectively control the pollution of the breeding environment. Compared with a single lytic phage, it has the characteristics of a wide lysis spectrum, which broadens the bactericidal range and improves the bactericidal activity.

The present application relates to the technical field of Salmonella bacteriophage, and particularly relates to a bacteriophage capable of lysing Salmonella Blegdam (Salmonella Blegdam bacteriophage) and a composition and application thereof. The bacteriophage is Salmonella bacteriophage RDP-SA-22010, the preservation number of which is CGMCC No. 45274, and the bacteriophage was preserved in the China General Microbiological Culture Collection Center on September 21, 2022. The Salmonella bacteriophage RDP-SA-22010 can also lyse Salmonella enteritidis, Salmonella pullorum and Salmonella kentucky. A composition comprises the Salmonella bacteriophage RDP-SA-22010. A reagent or kit comprises the above-mentioned composition. The present application also provides that the Salmonella bacteriophage RDP-SA-22010 can be applied to the preparation of a biological sterilizing agent, the preparation of a medicine for treating diseases caused by Salmonella and the preparation of a feed additive. The Salmonella bacteriophage RDP-SA-22010 in the present application provides a new biological control means for the prevention and treatment of Salmonella Blegdam.

The present invention belongs to the field of biotechnology, and particularly relates to a Salmonella phage SPYS_1 and its application. The present invention provides a Salmonella phage SPYS_1, which is deposited in the China Center for Type Culture Collection with the deposit number of CCTCC NO: M2024984. The Salmonella phage SPYS_1 of the present invention has strong tolerance to temperature and pH, adapts to various environments, proliferates rapidly, is suitable for large-scale production, and can effectively kill Salmonella, and can be used for the prevention or treatment of Salmonella infection in animal breeding.

The invention provides double probes for detecting salmonella, a kit as well as a preparation method and application of the kit, and belongs to the technical field of bacterial detection. The double probes comprise a capture probe and a detection probe; the capture probe is a magnetic bead coupled with a salmonella aptamer and a salmonella bacteriophage, and the detection probe is gold nanoparticles modified with fDNA; the salmonella aptamer and the fDNA are of a single-chain structure with mutually paired sequences. The double probes provided by the invention respectively promote the sensitive and convenient development of a salmonella detection technology through efficient capture, separation and enrichment and visual signal output. The scheme provided by the invention is simple to operate, low in cost, less in time consumption, easy to implement, high in sensitivity and high in accuracy, does not need large experimental equipment, and can realize visualization of detection results.

The invention discloses a salmonella bacteriophage nano-emulsion preparation as well as a preparation method and application thereof. The salmonella bacteriophage nano-emulsion preparation is mainly prepared from the following active ingredients in percentage by mass: 5 to 15 percent of salmonella bacteriophage suspension, 84 to 94 percent of edible oil, 0.5 to 3.0 percent of Pickering stabilizer dispersion liquid and 0 to 2.0 percent of protective agent. The invention also discloses a preparation method of the salmonella bacteriophage nano-emulsion preparation and application of the salmonella bacteriophage nano-emulsion preparation in bacteriostasis and preservation of meat products. The salmonella bacteriophage nano-emulsion preparation disclosed by the invention is more essential in stable mechanism, more stable in system, more beneficial to bacteriophage activity maintenance, better in food applicability, safe, friendly, more durable in bacteriostatic action and more suitable for fresh keeping of meat products when being applied.

The application discloses a Salmonella bacteriophage and application thereof, the bacteriophage is Salmonella bacteriophage TZCZ65, is preserved in China typical culture preservation center, CCTCC NO: M 20242879, the preservation date is December 23, 2024, and the preservation address is Wuhan.The Salmonella bacteriophage TZCZ65 disclosed in the application has good stability, no virulence drug resistance gene, high safety, has lysis activity to Salmonella of different sources, wide host range, short latent period, high burst size of progeny bacteriophage, and can be applied to livestock and poultry slaughtering, processing and breeding links, to provide a safe and efficient biological antibacterial product for livestock and poultry and products thereof.