21 results about "Bacterial diarrhea" patented technology

The invention relates to the technical field of functional microorganism screening and application, in particular to bacillus subtilis for preventing and treating bacterial diarrhea of pets and application of the bacillus subtilis. The preservation number of the bacillus subtilis is CCTCC (China Center for Type Culture Collection) NO: M20231592, and the bacillus subtilis has relatively strong acid resistance and cholate resistance, can effectively inhibit common pathogenic bacteria causing bacterial diarrhea and remarkably reduce the diarrhea rate of pets, and can be widely applied to production of pet foods or medicines.

The invention discloses a lactobacillus brevis with broad-spectrum bacteriostatic activity and a separation method and application thereof, and belongs to the technical field of microorganisms, in particular to a lactobacillus brevis AW04 with broad-spectrum bacteriostatic activity, which is characterized in that the lactobacillus brevis AW04 is preserved in the China Center for Type Culture Collection, the lactobacillus brevis AW04 is preserved in the China Center for Type Culture Collection, the lactobacillus brevis AW04 is preserved in the China Center for Type Culture Collection, and the lactobacillus brevis AW04 is preserved in the China Center for Type Culture Collection. The address is Wuhan University Campus, Wuchang District, Wuhan City, Hubei Province; the postcode is 430072; the preservation number of the strain is CCTCC M 20241970; the preservation date is September 13, 2024. The strain provides a novel, safe and efficient strain resource for developing microecological preparations, feed additives or veterinary drugs for preventing and treating bacterial diarrhea of animals such as calves and the like, and has important value for reducing the use of antibiotics in the breeding industry and coping with bacterial drug resistance crisis. The strain can be produced on a large scale by using the existing fermentation technology and is convenient to apply.

The invention discloses a quadruple PCR (Polymerase Chain Reaction) detection primer combination for detecting diarrhea pathogenic bacteria of young deer and application, and belongs to the technical field of biology. The quadruple PCR detection primer combination for detecting the diarrhea pathogenic bacteria of the young deer comprises an escherichia coli primer pair as shown in SEQ ID NO.1-2, a klebsiella pneumoniae primer pair as shown in SEQ ID NO.3-4, a salmonella primer pair as shown in SEQ ID NO.5-6 and a proteus mirabilis primer pair as shown in SEQ ID NO.7-8. The invention further discloses a kit for detecting the diarrhea pathogenic bacteria of the young deer. The multiple PCR detection method is established by using the specific genes of the main pathogenic bacteria of the diarrhea of the baby deer, so that the pathogenesis of the diarrhea of the baby deer can be quickly and accurately detected, the detection efficiency of epidemic diseases is greatly improved, and the method has important significance in preventing and treating bacterial diarrhea of the baby deer.

The invention belongs to the field of biological pharmacy and animal medicine, and particularly relates to a nano antibody of antibacterial hair adhesin protein CfaE as well as a preparation method and application of the nano antibody. The invention provides a nano antibody mVHH-IgA of antibacterial hair adhesin protein CfaE, the nano antibody at least comprises a VHH fragment, the VHH fragment comprises three amino acid fragments of CDR1, CDR2 and CDR3, and the amino acid sequence of the CDR1-3 is shown as SEQ ID NO.1-3 in sequence, or the amino acid sequence of the CDR1-3 is shown as SEQ ID NO.4-6 in sequence, and the nano antibody mVHH-IgA of the antibacterial hair adhesin protein CfaE is prepared from the nano antibody mVHH-IgA of the antibacterial hair adhesin protein CfaE, the nano antibody mVHH-IgA of the antibacterial hair adhesin protein CfaE and the nano antibody mVHH-IgA of the antibacterial hair adhesin protein CfaE. Meanwhile, the VHH fragment is also fused with a secreting type IgA alpha chain constant region. The nano antibody mVHH-IgA provided by the invention has high specificity and stability, the nano antibody mVHH-IgA as a feed additive is mixed with a conventional feed to feed animals, and animal experiments show that the animal bacterial diarrhea can be effectively treated by feeding the feed containing the nano antibody mVHH-IgA provided by the invention.

The invention provides a piglet health-care feed additive as well as a preparation method and application thereof, and belongs to the technical field of feed additives. The health-care feed additive for the piglets is prepared from the following raw materials in parts by weight: 30-50 parts of astragalus membranaceus, 30-50 parts of myrobalan, 25-40 parts of cynomorium songaricum, 25-40 parts of rhizoma alismatis, 25-40 parts of cortex lycii radicis, 20-30 parts of roasted rhizoma atractylodis macrocephalae, 20-30 parts of spina date seeds, 20-30 parts of bunge corydalis herb, 20-30 parts of acalypha australis, 10-20 parts of turmeric, 10-20 parts of paulownia leaves, 5-15 parts of loquat leaves, 3-5 parts of folium isatidis and 3-5 parts of hawthorn through processes such as fermentation, freeze drying and the like. The feed additive has the effects of regulating intestinal flora structures of piglets, increasing the number of beneficial bacteria in intestinal tracts, reducing field planting of harmful bacteria, reducing the occurrence rate of bacterial diarrhea of the piglets and the like, can replace antibiotics for use, does not remain harmful substances in pig bodies after being used, and is beneficial to realizing green and sustainable development of piglet breeding.

The application discloses a traditional Chinese medicine oral liquid for preventing and treating pig viral diarrhea disease and a preparation method and application thereof. The traditional Chinese medicine oral liquid comprises the following active components: honeysuckle, radix scutellariae, capillaris, viticis trifolia, gardenia, gentiana, salvia miltiorrhiza, dried tangerine or orange peel, radix notoginseng, ligustrum lucidum and liquorice. The traditional Chinese medicine oral liquid has the effects of clearing heat and resolving toxins, drying dampness and purging fire, invigorating the spleen and stomach, nourishing yin and generating fluid, and can be effectively used for preventing and treating pig viral diarrhea disease and bacterial diarrhea disease. The traditional Chinese medicine oral liquid has the characteristics of scientific prescription compatibility, wide raw material sources, simple preparation process, safe and wide application, and is suitable for industrialized breeding, and has clear efficacy, stable quality and strong market competitiveness.

The invention relates to the technical field of animal feed additives, in particular to a composition with bacteriostatic activity and application thereof. A screening antibacterial experiment of the plant essential oil and the organic acid shows that the composition of the plant essential oil and the organic acid, which is reasonably proportioned, has remarkable antibacterial activity on escherichia coli, salmonella typhimurium, shigella flexneri and clostridium perfringens, and particularly has a very good synergistic interaction effect. The composition of the plant essential oil and the organic acid is preferably thymol, carvacrol and sorbic acid, and the optimal compounding ratio is 3: 1: 1. The plant essential oil and organic acid composition provided by the invention has the advantages of being good in antibacterial effect, low in production cost, natural, non-toxic and the like, provides an effective treatment means for weaned piglets with bacterial diarrhea, and has huge potential commercial value.

This invention discloses a multilayer microsphere for the prevention and / or treatment of bacterial diarrhea, its preparation method, and its applications, relating to the field of biomedical technology. The multilayer microsphere has a three-layer core-shell structure: the core layer is loaded with *Lactobacillus plantarum* and sodium butyrate, the middle layer is a chitosan coating layer, and the outer layer is a sodium alginate coating layer. This invention has found that *Lactobacillus plantarum* and sodium butyrate have a significant synergistic antibacterial effect, effectively inhibiting the proliferation of pathogenic *Escherichia coli* and *Salmonella*. In vivo experiments have confirmed that the multilayer microsphere can effectively restore the body weight of diarrhea-prone animals, repair pathological damage to the jejunum and colon, and alleviate intestinal inflammation at the molecular level by inhibiting the activation of NF-κB and MAPK inflammatory pathways, downregulating the expression of pro-inflammatory factors IL-1β, IL-6, and TNF-α, and upregulating the level of the anti-inflammatory factor IL-10. The multilayer microsphere provided by this invention can serve as a novel, green, and safe formulation suitable for the prevention and control of bacterial diarrhea in livestock and poultry farming.

The invention discloses a traditional Chinese medicine composition for preventing and treating rabbit diarrhea as well as a preparation method and application of the traditional Chinese medicine composition. The traditional Chinese medicine composition is prepared from the following raw material medicines: cacumen biotae, tangerine leaves and dandelions. The rabbit diarrhea can be prevented and treated only through the small traditional Chinese medicine compound consisting of the three traditional Chinese medicines, the curative effect is clearer, side effects are avoided, and the safety is high. Through the effective means of screening the raw materials, optimizing the preparation process, improving the stability of the effective components and the like, the content and the stability of the effective components such as flavonoids, polysaccharides and organic acids in the composition are improved, and the rabbit diarrhea, especially the bacterial diarrhea, is effectively prevented and treated.

The invention discloses an extraction method and application of guava functional components for preventing and treating piglet diarrhea. The extraction method comprises the following steps: performing vacuum drying on guava leaves at 45-60 DEG C, and crushing; mixing with an ethanol-water mixed solvent, and carrying out ultrasonic-assisted extraction; filtering the extracting solution, concentrating under reduced pressure, and freeze-drying to obtain the guava leaf extract; according to the scheme, comprehensive prevention and control of bacterial diarrhea are achieved, phenolic compounds in GE directly inhibit growth of ETEC and salmonella, compound enzymes make up the defect that piglet digestive enzymes are insufficient to reduce intestinal burden, and traditional Chinese medicine compounds enhance the intestinal convergence function; the natural plant extract, the traditional Chinese medicine compound and the food-grade enzyme preparation are taken as core components, so that the problems of drug resistance and residue of antibiotics are avoided; ultrasonic-assisted ethanol-water mixed solvent extraction is adopted, and a freeze drying technology is combined, so that the yield of phenolic compounds in GE is increased, the impurity content is reduced, and the raw material cost and the feed palatability influence are reduced.

This invention provides a highly lytic Shigella phage and its applications, belonging to the field of bioengineering technology. The phage is named vB-SsoS-Q1, classified as Shigella sonnei phage, and deposited at the China General Microbiological Culture Collection Center (CGMCC) with accession number CGMCC No. 45414. This phage is a double-stranded DNA phage with an icosahedral head approximately 75 nm long and 70 nm wide, possessing a retractable tail. It belongs to the class Tailed Phages, with a full-length gene of 50326 bp. It does not contain virulence or drug resistance genes, exhibits highly efficient lytic ability, and can lyse broadly against Shigella and Escherichia coli. This phage can be applied to prevent and treat bacterial diarrhea in livestock and poultry, for the biopreservation of fresh meat, dairy products, and other foods, effectively controlling contamination by multidrug-resistant Shigella, and providing a safe and efficient new solution to replace antibiotics.

The invention belongs to the field of biological pharmacy and animal medicine, and particularly relates to a nano antibody of antibacterial hair adhesin protein CfaE as well as a preparation method and application of the nano antibody. The invention provides a nano antibody mVHH-IgA of antibacterial hair adhesin protein CfaE, the nano antibody at least comprises a VHH fragment, the VHH fragment comprises three amino acid fragments of CDR1, CDR2 and CDR3, the amino acid sequence of the CDR1 is shown as SEQ ID NO.1, the amino acid sequence of the CDR2 is shown as SEQ ID NO.2, the amino acid sequence of the CDR3 is shown as SEQ ID NO.3, and the nano antibody mVHH-IgA is fused with a secreting type IgA alpha chain constant region. The nano-antibody mVHH-IgA provided by the invention has high specificity and stability, the nano-antibody mVHH-IgA is used as a feed additive and is mixed with a conventional feed to feed animals, and animal experiments show that the feed containing the nano-antibody mVHH-IgA provided by the invention can be used for effectively treating porcine bacterial diarrhea.

The invention discloses an application of an METTL14 gene in resistance of sheep to escherichia coli infection. Research finds that the expression level of the METTL14 gene is closely related to escherichia coli resistance, the important effect of the METTL14 gene in the F17 escherichia coli infection resisting process of sheep is verified from mRNA, protein and cell levels, then the new application of the METTL14 gene is provided, it is determined that the METTL14 gene can be used as an important resistance candidate functional gene for bacterial diarrhea, and the application of the METTL14 gene in resisting F17 escherichia coli infection of sheep is promoted. And meanwhile, a scientific basis is provided for molecular breeding of the lambs for resisting the escherichia coli diarrhea disease in lamb production.

The invention relates to the technical field of traditional Chinese medicine compositions, in particular to a traditional Chinese medicine composition for preventing and treating chicken bacterial diarrhea, which is prepared from the following components in parts by weight: 20 to 50 parts of radix curcumae, 20 to 50 parts of cortex fraxini, 20 to 50 parts of honeysuckle stem, 40 to 80 parts of rheum officinale, 20 to 50 parts of fructus gardeniae, 10 to 30 parts of fructus chebulae, 10 to 30 parts of radix paeoniae alba and 10 to 30 parts of pericarpium zanthoxyli seed. The traditional Chinese medicine composition with different doses has a good clinical curative effect on chicken diarrhea caused by escherichia coli O78, and can relieve adverse effects of escherichia coli challenge on chicken by repairing tissue damage, regulating serum biochemical indexes and inflammatory factor content, improving intestinal morphology and reducing large intestine rod bacterium loading amount. According to the characteristics of multiple targets and multiple components of the compound traditional Chinese medicine, the effects of antibiosis, anti-inflammation, immunoregulation and the like are achieved, and meanwhile, the traditional Chinese medicine composition is nontoxic in clinical medication curative effect and safety evaluation and can be used for a long time.

The present invention relates to the technical field of postbiotic fermentation, and a preparation method and application of a postbiotic with inhibition of bacterial diarrhea pathogens are proposed, and the preparation method includes the following steps: S1, Lactobacillus paracasei Postbio-P8 and fermented mucus lactobacillus Postbio-Q8 strains are activated and cultured respectively, and then the activated Lactobacillus paracasei Postbio-P8 and fermented mucus lactobacillus Postbio-Q8 are fermented sequentially in stages, and after fermentation, the mixed fermentation liquid is sterilized to obtain a postbiotic fermentation liquid; S2, the postbiotic fermentation liquid is clarified and impurities removed, concentrated and desalted, and freeze-dried to obtain a postbiotic freeze-dried powder. The present invention adopts staged fermentation, which not only improves nutrient utilization efficiency, reduces the accumulation of metabolic inhibitors, but also alleviates space and competition with each other, thereby significantly improving the yield of metabolites such as total bacterial volume and organic acids.

The present invention provides a Chinese medicine compound for treating bacterial diarrhea and a preparation method and application thereof, and relates to the technical field of traditional Chinese medicine. The Chinese medicine compound of the present invention is prepared by crushing, extracting, concentrating and other steps using coptis root, phellodendron, scutellaria, atractylodes, stir-fried astragalus, roasted liquorice and Shenqu as raw materials. The Chinese medicine compound of the present invention has a therapeutic effect on bacterial diarrhea, has the advantages of being easy to use, low treatment cost, reducing inflammation, high survival rate, rapid recovery of health and the like, and has no toxic side effects, and can be used clinically.

The present invention discloses a Clostridium perfringens phage PM11, a phage composition thereof and an application thereof. The Clostridium perfringens phage PM11 for preventing and controlling poultry bacterial diarrhea has a preservation number of CGMCC No. 22372. The phage was deposited in the China General Microbiological Culture Collection Center on April 12, 2021. The present invention also discloses a phage composition formed by combining the Clostridium perfringens phage PM11 with Escherichia coli phage PD205 and Salmonella phage PC192. The above-mentioned Clostridium perfringens phage and phage composition can be used as an active ingredient for effectively preventing and treating poultry bacterial diarrhea, and can also be used as an environmental disinfectant to effectively control the pollution of the breeding environment. Compared with a single lytic phage, it has the characteristics of a wide lysis spectrum, which broadens the bactericidal range and improves the bactericidal activity.

The invention discloses a salmonella bacteriophage PC192, a bacteriophage composition and application of the salmonella bacteriophage PC192. The salmonella bacteriophage PC192 for preventing and controlling avian bacterial diarrhea has the preservation number of CGMCC No.22367. The salmonella bacteriophage PC192 is preserved in China General Microbiological Culture Collection Center on April 12, 2021, and the preservation number of the salmonella bacteriophage PC192 is CGMCC No.22367. The invention also discloses a bacteriophage composition formed by assembling the salmonella bacteriophage PC192, the escherichia coli bacteriophage PD205 and the clostridium welchii bacteriophage PM11, the salmonella bacteriophage PC192 and the bacteriophage composition can be used as active ingredients for effectively preventing and treating the poultry bacterial diarrhea, and can also be used as an environment disinfectant, so that the pollution of a breeding environment is effectively controlled, and the breeding cost is reduced. Compared with a single lytic bacteriophage, the bacteriophage has the characteristic of wide lysis spectrum, widens the bactericidal range and improves the bactericidal activity.

The invention discloses a dark plum antidiarrheal compound preparation for treating chicken diarrhea and a preparation method thereof. The dark plum antidiarrheal compound preparation is prepared from the following active ingredients: dark plum, schisandra chinensis, radix isatidis and berberis poiretii schneid in a mass ratio of (8-12): (12-16): (12-16): (8-12). The fructus mume antidiarrheal compound preparation for treating chicken diarrhea is low in price, and the cost of the fructus mume antidiarrheal compound preparation is reduced by 50% or above compared with that of currently common Chinese pulsatilla root oral liquid, Chinese pulsatilla root powder, Sihuang antidiarrheal granules, Siwei herba andrographitis powder and other compounds, and even is reduced by 500% or above compared with that of the Chinese pulsatilla root oral liquid / powder. The traditional Chinese medicine composition can completely inhibit characteristic pathological changes of peritonitis, pericarditis, perihepatic inflammation and enteritis caused by pathogenic bacteria of escherichia coli and salmonella by 100%, the cure rate of bacterial diarrhea is 100%, disordered intestinal flora and damaged intestinal villi of infected chickens can be recovered, and the inflammatory response of the infected chickens is remarkably reduced.

The invention relates to a traditional Chinese medicine composition for treating diarrhea and a preparation method and application thereof, and belongs to the technical field of traditional Chinese medicines. The invention provides a traditional Chinese medicine composition for treating diarrhea. The traditional Chinese medicine composition comprises Chinese fevervine herb and humifuse euphorbia herb. The mass ratio of the Chinese fevervine herb to the humifuse euphorbia herb is (1-2): (1-2). An escherichia coli infectious diarrhea mouse model is established, the prevention and treatment effects of independent application and combined application of the Chinese fevervine herb and the humifuse euphorbia herb on the escherichia coli infectious diarrhea are evaluated, and results show that the diarrhea symptoms are relieved to different degrees by independent application of the Chinese fevervine herb and the humifuse euphorbia herb, and the Chinese fevervine herb and the humifuse euphorbia herb have a synergistic effect; through combined application, the diarrhea degree can be effectively relieved, the body state can be improved, inflammation caused by diarrhea can be relieved, meanwhile, weight gain can be promoted, and an experimental basis is provided for scientific application of traditional Chinese medicine in prevention and treatment of animal bacterial diarrhea.

The invention discloses application of orange peel hydrolat in preparation of feed, feed additives and drugs. Relates to the technical field of plant extracts. The influence of the orange peel hydrolat on the growth performance, diarrhea rate, organ index, blood routine, serum antioxidant index and liver and jejunum structures and functions of the broiler chickens is researched, and the result shows that the growth performance of the broiler chickens can be remarkably improved and the incidence rate of diarrhea can be reduced by adding a certain dosage of orange peel hydrolat into the feed; meanwhile, the level of inflammatory factors is reduced, the level of immune factors and the activity of antioxidant enzymes are improved, inflammatory response and oxidative stress are reduced, and liver tissue and intestinal mucosa are protected, so that the integrity and functions of the liver and the intestinal tract are recovered. The results show that the broiler bacterial diarrhea can be effectively improved by adding a certain dose of orange peel hydrolat into the feed, and the mechanism of the orange peel hydrolat may be mainly related to the activation of the intestinal immune system and the reduction of inflammatory response.