Traditional Chinese medicine composition for preventing and treating rabbit diarrhea as well as preparation method and application of traditional Chinese medicine composition

Abstract

The invention discloses a traditional Chinese medicine composition for preventing and treating rabbit diarrhea as well as a preparation method and application of the traditional Chinese medicine composition. The traditional Chinese medicine composition is prepared from the following raw material medicines: cacumen biotae, tangerine leaves and dandelions. The rabbit diarrhea can be prevented and treated only through the small traditional Chinese medicine compound consisting of the three traditional Chinese medicines, the curative effect is clearer, side effects are avoided, and the safety is high. Through the effective means of screening the raw materials, optimizing the preparation process, improving the stability of the effective components and the like, the content and the stability of the effective components such as flavonoids, polysaccharides and organic acids in the composition are improved, and the rabbit diarrhea, especially the bacterial diarrhea, is effectively prevented and treated.

Description

Technical Field

[0001] The invention belongs to the technical field of traditional Chinese medicines, and in particular relates to a traditional Chinese medicine composition for preventing and treating rabbit diarrhea, and a preparation method and application thereof. Background Art

[0002] In the process of intensive rabbit breeding, diarrhea in young rabbits is one of the most common diseases that harm rabbits. In particular, the mortality rate of weaned rabbits is as high as 50-70%, of which more than 80% is caused by diarrhea. Rabbits have poor tolerance to diarrhea. Once the disease occurs, it will cause large casualties and cause serious economic losses to farmers. The control of diarrhea in young rabbits will directly affect the growth rate and feed conversion rate during the entire breeding period, and ultimately affect the economic benefits of the rabbit farm.

[0003] There are many factors that cause diarrhea in rabbits, including non-biological factors such as climate change, weaning, and feed changes, and biological factors such as Clostridium perfringens, Escherichia coli, rotavirus, and coccidia. The most common one is bacterial diarrhea. The use of antibiotics is the most common method to prevent and treat diarrhea in rabbits, but it also brings many negative effects, such as destroying the balance of microbial flora in the cecum of rabbits. After people eat rabbit meat products with residual antibiotics, they passively develop drug resistance, which endangers human health. Therefore, the country has banned the addition of growth-promoting antibiotics to feed. For this reason, it is a general trend to prepare alternative drugs with similar efficacy to antibiotics for the prevention and treatment of diarrhea in rabbits. However, the pure Chinese medicine preparations currently developed for the treatment of diarrhea in rabbits are all large compound prescriptions, which are more suitable for comprehensive treatment of complex diseases with multiple targets, but cannot be accurately treated for a single disease. Moreover, since the large compound prescription is composed of multiple Chinese medicinal materials and has complex ingredients, it will increase the absorption burden of the gastrointestinal tract, stimulate the gastrointestinal mucosa, and is not conducive to the treatment of diarrhea. Summary of the invention

[0004] In view of the above shortcomings, the present invention provides a Chinese medicine composition for preventing and treating rabbit diarrhea, and its preparation method and application. The composition is composed of Platycladus orientalis leaves, Citrus aurantium leaves and Taraxacum officinale, and can be used to prevent and treat rabbit diarrhea, especially for bacterial diarrhea.

[0005] In order to solve the problems existing in the prior art, the present invention adopts the following technical solutions:

[0006] In a first aspect, the present invention provides a traditional Chinese medicine composition for preventing and treating rabbit diarrhea, which is prepared from the following raw materials: Platycladus orientalis leaves, Citrus aurantium leaves, and Taraxacum officinale.

[0007] Furthermore, the Chinese medicine composition is composed of the following raw materials in the following weight ratio: Platycladus orientalis leaf: Citrus aurantium leaf: Taraxacum officinale = 2:1-2:1-2.

[0008] Preferably, the Chinese medicine composition is composed of the following raw materials in the following weight ratio: Platycladus orientalis leaf: Citrus aurantium leaf: Taraxacum officinale = 2:2:1.

[0009] In a second aspect, the present invention provides use of the Chinese medicine composition described in the first aspect in the preparation of a medicament for preventing and treating diarrhea in rabbits.

[0010] In a third aspect, the present invention provides a medicine for preventing and treating diarrhea in rabbits, comprising the Chinese medicine composition described in the first aspect.

[0011] Furthermore, the dosage form of the drug is a solution or granules.

[0012] In a fourth aspect, the present invention provides a method for preparing the drug for preventing and treating rabbit diarrhea according to the third aspect, comprising the following steps:

[0013] (1) Drying Platycladus orientalis leaves, tangerine leaves, and dandelions;

[0014] (2) Add purified water at a solid-liquid ratio of 1:15-25 g / ml and boil for 1-3 times, each time for 1-3 hours, combine the filtrates, and concentrate the filtrates to obtain a solution.

[0015] In a fifth aspect, the present invention provides a method for preparing the drug for preventing and treating rabbit diarrhea according to the third aspect, comprising the following steps:

[0016] (1) Drying Platycladus orientalis leaves, tangerine leaves, and dandelions;

[0017] (2) adding purified water at a solid-liquid ratio of 1:15-25 g / ml and boiling for 1-3 times, each time for 1-3 hours, combining the filtrates, and concentrating the filtrates;

[0018] (3) adding anhydrous ethanol, standing for 23-25 ​​hours, filtering, removing the solvent, and concentrating to an extract to obtain a Chinese medicine extract powder;

[0019] (4) Adding a filler to the Chinese medicine extract powder, mixing evenly, adding a binder, stirring to granulate, and drying to obtain Chinese medicine granules.

[0020] Furthermore, the filler is lactose, dextrin or mannitol.

[0021] Furthermore, the adhesive is anhydrous ethanol.

[0022] The advantages and beneficial effects of the present invention are:

[0023] The leaves of Platycladus orientalis are the leaves of Cupressus funebris Endl. of the Cupressaceae family. The cypress is distributed in the southwest and Shaanxi, Gansu, Zhejiang, Jiangxi, Fujian and other places, with the largest distribution in Sichuan, western Hubei and Guizhou. The leaves of Platycladus orientalis are bitter, astringent, slightly cold, astringent and dry in nature, and have the effects of clearing heat and cooling blood, dispelling wind and removing dampness, stopping bleeding and removing blood stasis. It is often used to stop bleeding, treat burns, vomiting blood, bloody diarrhea, hemorrhoids and other diseases. The active ingredients of Platycladus orientalis leaves are mainly flavonoids, volatile oils, polysaccharides and tannins, which have anti-inflammatory, hemostatic, antibacterial and antioxidant pharmacological activities.

[0024] Tangerine leaves are the leaves of the citrus plant of the Rutaceae family. They are mainly distributed in more than 10 provinces (cities) in my country, including Zhejiang, Fujian, Sichuan, Guangxi and Guangdong. Tangerine leaves are flat in nature, bitter and spicy in taste, and have the effects of soothing the liver and promoting qi, regulating qi and relieving pain, and dispersing knots. They are mainly used to treat carbuncle pain, hernia pain, etc., and there are medicinal records in herbal classics such as "Compendium of Materia Medica". Chemical composition studies have shown that the effective chemical components of tangerine leaves are mainly flavonoids, volatile oils and carbohydrates, among which polymethoxyflavonoid components such as citrus nobiletin and tangerine have pharmacological activities such as antioxidant, antiviral, antibacterial, anti-inflammatory and neuroprotective. In addition to utilizing the anti-inflammatory and antibacterial activity of flavonoids, the addition of tangerine leaves to this recipe can also relieve the pain of animals with diarrhea.

[0025] Dandelion is a perennial herb belonging to the Asteraceae family. The whole plant of dandelion grows widely in hillside meadows, roadsides, fields and riverbanks in medium and low altitude areas. The whole plant of dandelion can be used as medicine. It is sweet, slightly bitter and cold in nature. It has diuretic, laxative, jaundice-relieving and choleretic effects. The active ingredients of dandelion are taraxacin, taraxacin, choline, organic acids and inulin, which have antioxidant, antibacterial and anti-inflammatory activities, inhibit intestinal inflammation and ulcer lesions, and treat acute and chronic inflammation.

[0026] For rabbit bacterial diarrhea, Western medicine mainly uses antidiarrheal drugs, antibiotics and other drugs to treat bacterial infections. Chinese veterinary medicine classifies diarrhea as diarrhea, and often uses the method of clearing heat and detoxification. Clinically, rabbits show mental anxiety, loss of appetite, thirst and love to drink, thin feces, yellow or green, sometimes with blood or mucus, abdominal pain and bloating.

[0027] There are many kinds of Chinese medicinal materials. Under the premise that the efficacy cannot be simply expected, the present invention creatively combines three Chinese medicines, Platycladus orientalis, tangerine leaves, and dandelions, in a specific ratio to achieve a good therapeutic effect of preventing and treating bacterial diarrhea in rabbits. Platycladus orientalis is the main medicine, which has the effects of hemostasis, anti-inflammatory, and antibacterial; the ministerial medicine is tangerine leaves, which has the effects of promoting qi, antibacterial, and analgesia; the adjuvant is dandelions, which has anti-inflammatory and antibacterial effects. From the comparison of the embodiment and the comparative example, it can be seen that Platycladus orientalis, tangerine leaves, and dandelions show a good synergistic therapeutic effect after being combined in a specific ratio, and bacterial diarrhea is accurately treated to achieve the best therapeutic effect. The present invention can achieve the prevention and treatment of rabbit diarrhea only through a small Chinese medicine compound composed of three Chinese medicines, and the efficacy is clearer, there is no side effect, and the safety is high. The present invention improves the content and stability of effective ingredients such as flavonoids, polysaccharides, and organic acids in the composition through effective means such as raw material screening, preparation process optimization, and improving the stability of effective ingredients, and effectively prevents and treats rabbit diarrhea, especially bacterial diarrhea. The invention is aimed at treating bacterial diarrhea in rabbits and has the characteristics of high efficiency, no residue, low cost, good safety, no drug resistance and no toxic side effects. DETAILED DESCRIPTION

[0028] The technical solutions in the embodiments of the present application are clearly described below. Obviously, the described embodiments are part of the embodiments of the present application, rather than all of the embodiments. All other embodiments obtained by ordinary technicians in this field based on the embodiments in the present application belong to the scope of protection of the present application.

[0029] The experimental methods in the following examples, unless otherwise specified, are all conventional methods, and are performed according to the techniques or conditions described in the literature in the field or according to the product instructions. The materials, reagents, etc. used in the following examples, unless otherwise specified, can all be obtained from commercial channels.

[0030] Example 1

[0031] This embodiment provides a Chinese medicine composition granule for preventing and treating rabbit diarrhea, which is prepared from the following raw materials: 50g of Platycladus orientalis leaves, 50g of Citrus aurantium leaves, and 25g of Taraxacum officinale.

[0032] The arborvitae leaves, tangerine leaves and dandelions are all produced in the hilly areas of Sichuan. The present invention uses the arborvitae leaves, tangerine leaves and dandelions produced in the hilly areas of Sichuan as raw materials, the effective ingredients can be better retained, and the medicinal raw materials are easy to obtain.

[0033] This embodiment provides a method for preparing a granule of a Chinese medicine composition for preventing and treating diarrhea in rabbits, comprising the following steps:

[0034] (1) Drying the Platycladus orientalis leaves, orange leaves, and dandelions at 60° C. for 4 h. Drying at 60° C. in this step is beneficial to the efficacy of the medicine;

[0035] (2) Add purified water at a solid-liquid ratio of 1:20 g / ml and boil twice, each time for 2 h, combine the filtrates, and concentrate the extract to 1 g / ml, which is equivalent to 1 g of the original drug per ml of solution;

[0036] The inventors have found through experiments that by optimizing the process conditions, the extraction rate and extraction quality can be increased when the solid-liquid ratio is 1:15-25g / ml. Among them, when the solid-liquid ratio is 1:20, the extraction rate and extraction quality are the best.

[0037] (3) adding anhydrous ethanol until the volume percentage of anhydrous ethanol is 60%, standing for 24 hours, filtering, and removing the solvent by rotary evaporation of the filtrate, wherein the rotary evaporation temperature is 60° C. and the speed is 80 r / min, and concentrating to an extract to obtain a Chinese medicine extract powder. In this step, the rotary evaporation is carried out at a temperature of 60° C. and a speed of 80 r / min, which is conducive to exerting the efficacy of the medicine. If the rotary evaporation temperature is too high or too low, the effective ingredients will be lost.

[0038] (4) Weigh lactose and Chinese medicine extract powder in a mass ratio of 1:1, pass through an 80-mesh sieve, mix evenly, add anhydrous ethanol as a binder until the volume percentage of anhydrous ethanol is 60%, stir to granulate, dry at 60°C for 30min, pass through an 18-mesh sieve to obtain Chinese medicine granules.

[0039] The present application can use lactose, dextrin or mannitol as fillers. However, the inventors have found through experiments that lactose is the best filler because it is more stable, has the right hardness and softness, is palatable, and releases drugs faster when combined with other raw materials.

[0040] Example 2

[0041] This embodiment provides a Chinese medicine composition granule for preventing and treating rabbit diarrhea, which is prepared from the following raw materials: 50g of Platycladus orientalis leaves, 50g of Citrus aurantium leaves, 50g of Taraxacum officinale, and the rest are the same as in Example 1.

[0042] Example 3

[0043] This embodiment provides a Chinese medicine composition granule for preventing and treating rabbit diarrhea, which is prepared from the following raw materials: 50g of Platycladus orientalis leaves, 25g of Citrus aurantium leaves, 50g of Taraxacum officinale, and the rest are the same as in Example 1.

[0044] Example 4

[0045] This embodiment provides a Chinese medicine composition solution for preventing and treating diarrhea in rabbits, which is prepared from the following raw materials: 50g of Platycladus orientalis leaves, 50g of Citrus aurantium leaves, and 25g of Taraxacum officinale.

[0046] This embodiment provides a method for preparing a solution of a Chinese medicine composition for preventing and treating diarrhea in rabbits, comprising the following steps:

[0047] (1) Dry Platycladus orientalis leaves, orange leaves, and dandelions at 60°C for 4 h;

[0048] (2) Purified water was added at a solid-liquid ratio of 1:20 g / ml and boiled twice, each time for 2 h. The filtrates were combined and the extract was concentrated to 1 g / ml, which is equivalent to 1 g of the original drug per ml of solution.

[0049] Comparative Example 1

[0050] This comparative example provides a Chinese medicine composition for preventing and treating rabbit diarrhea. The difference between this comparative example and Example 1 is only the lack of the component dandelion, and the rest is the same as Example 1.

[0051] Comparative Example 2

[0052] This comparative example provides a Chinese medicine composition for preventing and treating rabbit diarrhea. The difference between this comparative example and Example 1 is only the lack of the component tangerine leaf, and the rest is the same as Example 1.

[0053] Comparative Example 3

[0054] This comparative example provides a Chinese medicine composition for preventing and treating rabbit diarrhea. The difference between this comparative example and Example 1 is only that the amount of Platycladus orientalis leaves used is 100g, and the rest is the same as Example 1.

[0055] Comparative Example 4

[0056] The drug used in this comparative example for preventing and treating rabbit diarrhea is enrofloxacin.

[0057] Comparative Example 5

[0058] This comparative example provides a Chinese medicine composition for preventing and treating diarrhea in rabbits. The difference between this comparative example and Example 1 is only that Purslane (dried aerial part) is used instead of Platycladus orientalis leaves, and the rest is the same as Example 1.

[0059] Experimental Example 1 Test on the effect of Chinese medicine composition in preventing diarrhea in rabbits

[0060] 1 Experimental animals

[0061] This experiment was conducted in two farms with similar feeding conditions in Suining City from November 2023 to February 2024 as a replicate experiment. 120 New Zealand white rabbits, 35 days old and weighing about 800g, were selected from the farms, half of them were male and half were female.

[0062] 2 Methods

[0063] 2.1 Test methods

[0064] The experimental rabbits were concentrated in the same rabbit house with overlapping three-layer cages, with 2 rabbits in each cage. Each group of 6 rabbits was marked separately, and a total of 10 groups were set. One group of rabbits was fed with the medicine prepared in Example 1, two groups of rabbits were fed with the medicine prepared in Example 2, three groups of rabbits were fed with the medicine prepared in Example 3, four groups of rabbits were fed with the medicine prepared in Example 4, five groups of rabbits were fed with the medicine prepared in Comparative Example 1, six groups of rabbits were fed with the medicine prepared in Comparative Example 2, seven groups of rabbits were fed with the medicine prepared in Comparative Example 3, eight groups of rabbits were fed with the medicine prepared in Comparative Example 4, nine groups of rabbits were fed with the medicine in Comparative Example 5, the tenth group was a blank control group (no medicine was used), and the eight groups (Comparative Example 4) used a dosage of 2‰ enrofloxacin powder uniformly mixed in the commercially available baby rabbit basic feed, and the medicines of the remaining groups except the eight groups were mixed into the commercially available baby rabbit basic feed according to a mixed feeding ratio of 1.0% by mass. All groups were pre-fed 7 days before weaning, and the formal prevention test began on the day of weaning, and the formal test lasted for 14 days. The rabbit house was cleaned and disinfected regularly. All rabbits were vaccinated and dewormed according to the immunization program of the farm. During the experiment, they were free to eat and drink, and clinical observations and records were conducted. The feeding and management conditions remained consistent during the experiment.

[0065] 2.2 Effect judgment criteria

[0066] Observe the appetite, coat color, mental state, perianal moisture and feces of the puppies regularly every day, and record the number of diarrhea and deaths in each cage of each group in a timely manner.

[0067] Diarrhea rate (%) = (number of diarrheal animals * number of diarrheal days) / (number of test animals * number of test days) * 100

[0068] Mortality rate (%) = number of deaths / number of tested animals*100.

[0069] 3 Test results

[0070] 3.1 The results of the diarrhea prevention test are shown in Table 1:

[0071] Table 1

[0072]

[0073]

[0074] During the test period, one rabbit died of diarrhea in the blank control group 1, zero rabbit died of diarrhea in the blank control group 2, and no rabbit died in the example groups 1 to 4 and the comparative example groups 1 to 5. The average mortality rate of the blank control group was 8.33%.

[0075] As can be seen from Table 1, the Chinese medicine compositions prepared in Examples 1-4 of the present invention all have good effects in preventing rabbit diarrhea, and are superior to the preventive effects of all comparative groups. The Chinese medicine composition granules prepared in Example 1 have the best effect, with a preventive effect of 100% on rabbit diarrhea, while the preventive effect of Example 2 on rabbit diarrhea is 97.62%; and the preventive effect of Example 3 on rabbit diarrhea is 96.43%.

[0076] From the comparison between the embodiment and the comparative example, it can be seen that the Chinese medicine compositions prepared by compounding Platycladus orientalis leaves with tangerine leaves and dandelions in a weight ratio of 2:1-2:1-2 all have a good effect of preventing rabbit diarrhea, among which the composition composed of Platycladus orientalis leaves with tangerine leaves and dandelions in a weight ratio of 2:2:1 has the best effect. This shows that Platycladus orientalis leaves, tangerine leaves and dandelions have a synergistic effect after being mixed in a specific ratio, which enhances the effect of preventing rabbit diarrhea.

[0077] The effect of the Chinese medicine composition solution prepared in Example 4 is inferior to that in Example 1, and the prevention effect of rabbit diarrhea is 99.405%. This may be because the preparation method of Example 1 is more conducive to the extraction of effective ingredients such as flavonoids in Platycladus orientalis leaves and effective ingredients in dandelions and orange leaves, which shows that after the raw materials of the present application are combined in a specific proportion, under a specific preparation method, the components will produce a synergistic effect, thereby improving the effect of preventing rabbit diarrhea.

[0078] The Chinese medicine composition prepared in Comparative Example 1 has a preventive effect of 95.24% on rabbit diarrhea. Due to the lack of the component dandelion, the synergistic effect cannot be well exerted, resulting in a reduced preventive effect.

[0079] The Chinese medicine composition prepared in Comparative Example 2 has a preventive effect of 95.24% on rabbit diarrhea. The preventive effect is relatively low, and due to the lack of orange leaves, the synergistic effect cannot be well exerted, resulting in a reduced preventive effect.

[0080] The Chinese medicine prepared in Comparative Example 3 has a preventive effect of 94.64% on rabbit diarrhea, which is relatively low. This is because the content of Platycladus orientalis leaves is too high, resulting in insufficient antibacterial and anti-inflammatory effects, which makes the preventive effect worse.

[0081] The preventive effect of the antibiotic group in Comparative Example 4 on rabbit diarrhea was 94.05%, and the rabbits were particularly prone to relapse and drug resistance.

[0082] The Chinese medicine prepared in Comparative Example 5 has a preventive effect of 89.88% on rabbit diarrhea, which is poor. This is because Purslane is used instead of Platycladus orientalis leaves, and Purslane does not have a synergistic effect with the other two medicines, resulting in a poor preventive effect.

[0083] The comparison between Examples 1-4 and Comparative Examples 1-5 indicates that the composition of Platycladus orientalis leaves, citrus leaves and dandelion of the present invention exhibits a synergistic effect in preventing rabbit diarrhea.

[0084] Experimental Example 2: Effect test of Chinese medicine composition in treating diarrhea in rabbits

[0085] 1 Experimental animals

[0086] This experiment was conducted in a farm within the jurisdiction of Suining City from November 2023 to February 2024. 120 New Zealand white rabbits of about 30-40 days old and weighing about 800g were selected from the same batch of rabbits. They were diagnosed with bacterial diarrhea clinically and in the laboratory. In the early stage of the disease, the clinical symptoms were mental anxiety, loss of appetite, thin feces, translucent colloid on the surface, yellow or green in color; among them, there were 40 rabbits with blood in the feces, abdominal pain and bloating and other severe symptoms, which were respectively set in each experimental group, 4 in each group. Centrally isolated in the same rabbit house with overlapping three-layer cages, 2 in each cage. Each group of 12 was marked separately, and a total of 10 groups were set up.

[0087] 2 Methods

[0088] 2.1 Test methods

[0089] One group of rabbits was fed with the medicine prepared in Example 1, two groups of rabbits were fed with the medicine prepared in Example 2, three groups of rabbits were fed with the medicine prepared in Example 3, four groups of rabbits were fed with the medicine prepared in Example 4, five groups of rabbits were fed with the medicine prepared in Comparative Example 1, six groups of rabbits were fed with the medicine prepared in Comparative Example 2, seven groups of rabbits were fed with the medicine prepared in Comparative Example 3, eight groups of rabbits were fed with the medicine in Comparative Example 4, and nine groups of rabbits were fed with the medicine in Comparative Example 5. A blank control group (no medicine was used). The eight groups (Comparative Example 4) used a dose of 2‰ enrofloxacin powder uniformly mixed in the commercially available rabbit basal diet, and the medicines of the remaining groups except the eight groups were mixed into the commercially available rabbit basal diet according to a mixing ratio of 3% by mass. 7 days is a course of treatment. The rabbit house was cleaned and disinfected regularly. All rabbits were vaccinated and dewormed according to the immunization program of the field. During the experiment, they were free to eat and drink water, and clinical observation and records were carried out. The feeding and management conditions remained consistent during the experiment.

[0090] 2.2 Effect judgment criteria

[0091] Observe the appetite, coat color, mental state, perianal moisture and feces of the puppies regularly every day, and record the number of diarrhea and deaths in each cage of each group in a timely manner.

[0092] Cure standard: After treatment, the sick rabbit's spirit, eating, defecation and other behaviors return to normal, and it is judged to be cured.

[0093] Mortality rate (%) = number of deaths / number of tested animals*100.

[0094] 3 Test results

[0095] 3.1 The test results are shown in Table 2:

[0096] Table 2

[0097] Group Quantity(Head) Number of deaths mortality rate(%) Cure rate (%) Example 1 12 0 0 100 Example 2 12 1 8.3 91.7 Example 3 12 2 16.67 83.33 Example 4 12 1 8.3 91.7 Comparative Example 1 12 3 25 75 Comparative Example 2 12 4 33.33 66.67 Comparative Example 3 12 3 25 75 Comparative Example 4 12 4 33.33 66.67 Comparative Example 5 12 6 50 50 Blank control 12 8 66.67 33.33

[0098] As can be seen from Table 2, the Chinese medicine compositions prepared in Examples 1-4 of the present invention have a good effect in treating bacterial diarrhea in rabbits, and are superior to the therapeutic effects of all comparative examples. Among them, the Chinese medicine composition granules prepared in Example 1 have the best effect, with a cure effect of 100% for bacterial diarrhea in rabbits, and no drug residue and no drug resistance. This shows that after the raw materials of the present application are combined in a specific ratio, under a specific preparation method, a synergistic effect will be generated between the components, thereby improving the effect of preventing bacterial diarrhea in rabbits.

[0099] From the comparison between the embodiment and the comparative example, it can be seen that the Chinese medicine compositions prepared by compounding Platycladus orientalis leaves with tangerine leaves and dandelions in a weight ratio of 2:1-2:1-2 all have good effects on treating bacterial diarrhea in rabbits, among which the composition compounded with Platycladus orientalis leaves with tangerine leaves and dandelions in a weight ratio of 2:2:1 has the best therapeutic effect. This shows that Platycladus orientalis leaves, tangerine leaves and dandelions have a synergistic effect after being mixed in a specific ratio, which enhances the effect of treating bacterial diarrhea in rabbits.

[0100] The Chinese medicine composition solution prepared in Example 4 has a therapeutic effect of 91.7% on bacterial diarrhea in rabbits, which is inferior to that in Example 1. This may be because the preparation method of Example 1 is more conducive to the extraction of effective ingredients such as flavonoids in Platycladus orientalis leaves, and effective ingredients in dandelions and orange leaves. This shows that after the raw materials of the present application are combined in a specific proportion, under a specific preparation method, a synergistic effect will be produced between the components, thereby improving the effect of treating bacterial diarrhea in rabbits.

[0101] The therapeutic effect of the Chinese medicine composition prepared in Comparative Example 1 on bacterial diarrhea in rabbits is 75%. Due to the lack of the component dandelion, the synergistic effect cannot be well exerted, resulting in a reduced therapeutic effect.

[0102] The therapeutic effect of the Chinese medicine composition prepared in Comparative Example 2 on bacterial diarrhea in rabbits is 66.67%. The therapeutic effect is low because the flavonoid active ingredients of Platycladus orientalis leaves cannot synergize with the active ingredients of tangerine leaves, resulting in a reduced therapeutic effect.

[0103] The therapeutic effect of the traditional Chinese medicine prepared in Comparative Example 3 on rabbit diarrhea is 75%, which is relatively low. This is because the content of Platycladus orientalis leaves is too high, resulting in insufficient antibacterial and anti-inflammatory effects, which makes the therapeutic effect worse.

[0104] The therapeutic effect of the antibiotic group in Comparative Example 4 on rabbit diarrhea was 66.67%, and the rabbits were particularly prone to relapse and drug resistance.

[0105] The therapeutic effect of the Chinese medicine prepared in Comparative Example 5 on rabbit diarrhea is 50%, which is poor. This is because Purslane is used instead of Platycladus orientalis leaves, and Purslane does not have a synergistic effect with the other two medicines, resulting in a poor therapeutic effect.

[0106] The comparison between Examples 1-4 and Comparative Examples 1-5 indicates that the composition of Platycladus orientalis leaves, Citrus aurantium leaves and Taraxacum officinale of the present invention exhibits a synergistic effect in treating bacterial diarrhea in rabbits.

[0107] This experiment selects rabbits of the same breed, similar weight, similar feeding and management as experimental animals, thereby avoiding the difference in results caused by the participation of experimental animals. The raw materials used in the present invention are all common Chinese medicines, with low cost and high safety, and are suitable for the prevention and treatment of rabbit diarrhea in large-scale breeding.

[0108] The present application is described above in conjunction with the embodiments, but the present application is not limited to the above-mentioned specific implementation modes, which are merely illustrative and not restrictive. Under the guidance of the present application, ordinary technicians in this field can make many forms without departing from the purpose of the present application and the scope of protection of the claims, all of which are within the protection of the present application.

Claims

1. A Chinese medicine composition for preventing and treating diarrhea in rabbits, characterized in that: It is prepared from the following raw materials: Platycladus orientalis leaves, citrus leaves, and dandelion.

2. A Chinese medicine composition for preventing and treating diarrhea in rabbits according to claim 1, characterized in that: The Chinese medicine composition is composed of the following raw materials in the following weight ratio: Platycladus orientalis leaf: Citrus aurantium leaf: Taraxacum officinale = 2:1-2:1-2.

3. A Chinese medicine composition for preventing and treating diarrhea in rabbits according to claim 2, characterized in that: The Chinese medicine composition is composed of the following raw materials in the following weight ratio: Platycladus orientalis leaf: Citrus aurantium leaf: Taraxacum officinale = 2:2:

1.

4. Use of the Chinese medicine composition according to any one of claims 1 to 3 in the preparation of a medicament for preventing and treating diarrhea in rabbits.

5. A drug for preventing and treating diarrhea in rabbits, characterized in that: A Chinese medicine composition comprising any one of claims 1 to 3.

6. The drug according to claim 5, characterized in that: The dosage form of the drug is solution or granules.

7. The method for preparing the drug for preventing and treating rabbit diarrhea according to claim 6, characterized in that: The following steps are involved: (1) Drying Platycladus orientalis leaves, orange leaves and dandelions; (2) Add purified water at a solid-liquid ratio of 1:15-25 g / ml and boil for 1-3 times, each time for 1-3 hours. Combine the filtrates and concentrate the filtrates to obtain a solution.

8. The method for preparing the drug for preventing and treating rabbit diarrhea according to claim 6, characterized in that: The following steps are involved: (1) Drying Platycladus orientalis leaves, orange leaves and dandelions; (2) Add purified water at a solid-liquid ratio of 1:15-25 g / ml and boil for 1-3 times, each time for 1-3 hours, combine the filtrate, and concentrate the filtrate; (3) Add anhydrous ethanol, let stand for 23-25 ​​hours, filter, remove the solvent, and concentrate to obtain extract to obtain Chinese medicine extract powder; (4) Add filler to the Chinese medicine extract powder, mix well, add binder, stir to granulate, and dry to obtain Chinese medicine granules.

9. The method for preparing the drug for preventing and treating rabbit diarrhea according to claim 8, characterized in that: The filler is lactose, dextrin or mannitol.

10. The method for preparing the drug for preventing and treating rabbit diarrhea according to claim 8, characterized in that: The adhesive is anhydrous ethanol.

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