Dark plum antidiarrheal compound preparation for treating chicken diarrhea and preparation method thereof

Abstract

The invention discloses a dark plum antidiarrheal compound preparation for treating chicken diarrhea and a preparation method thereof. The dark plum antidiarrheal compound preparation is prepared from the following active ingredients: dark plum, schisandra chinensis, radix isatidis and berberis poiretii schneid in a mass ratio of (8-12): (12-16): (12-16): (8-12). The fructus mume antidiarrheal compound preparation for treating chicken diarrhea is low in price, and the cost of the fructus mume antidiarrheal compound preparation is reduced by 50% or above compared with that of currently common Chinese pulsatilla root oral liquid, Chinese pulsatilla root powder, Sihuang antidiarrheal granules, Siwei herba andrographitis powder and other compounds, and even is reduced by 500% or above compared with that of the Chinese pulsatilla root oral liquid / powder. The traditional Chinese medicine composition can completely inhibit characteristic pathological changes of peritonitis, pericarditis, perihepatic inflammation and enteritis caused by pathogenic bacteria of escherichia coli and salmonella by 100%, the cure rate of bacterial diarrhea is 100%, disordered intestinal flora and damaged intestinal villi of infected chickens can be recovered, and the inflammatory response of the infected chickens is remarkably reduced.

Description

Technical Field

[0001] This invention relates to the field of traditional Chinese veterinary medicine technology, and in particular to a compound preparation of dried plum for treating chicken diarrhea and its preparation method. Background Technology

[0002] Chicken diarrhea is a common and prevalent multifactorial disease in large-scale, intensive, and standardized chicken farming, and a significant cause of chick mortality. The main causes of chicken diarrhea include stress, viruses, parasites, and bacterial infections. Bacterial diarrhea, especially that caused by E. coli and Salmonella, is frequently observed in large-scale farms. Based on the clinical symptoms and pathological changes of chicken colibacillosis and Salmonella infection, it can be diagnosed as a damp-heat syndrome in traditional Chinese veterinary medicine. Treatment with herbal formulas often focuses on clearing heat and detoxifying, and resolving dampness and stopping diarrhea. Currently, a variety of traditional Chinese medicine formulas for treating chicken diarrhea have been published, such as Pulsatilla chinensis oral liquid, Pulsatilla chinensis powder, Qingjie compound, Sihuang Zhili granules, Siwei Chuanxinlian powder, Yangshuhua oral liquid, Qiqing Baidu granules, Shuangge Zhixie oral liquid, compound plum decoction, Jiawei Sanhuang decoction, compound berberine tablets, Zhili pills, Huangbai Zhili granules, Jili Ling powder, Gegen Qinlian decoction, Shuanghuanglian compound, Bailong granules, Sanhuang Jiabai powder, and plum compound, etc.

[0003] However, all of the aforementioned traditional Chinese medicine formulas for treating chicken diarrhea use relatively expensive herbs, such as Pulsatilla chinensis, Coptis chinensis, and Lonicera japonica, and these herbs are used as the main ingredients in large doses. According to statistics (data from January 2024 to July 2025), the price of these three herbs ranges from 80 to 350 yuan / kg. Some compound formulas even contain more than two of these expensive herbs, with an estimated total price exceeding 60 yuan / kg. Clinically, large doses are commonly used for treating or preventing diseases with traditional Chinese medicine, making it difficult to widely adopt compound formulas costing 60 yuan / kg.

[0004] At present, the aquaculture industry faces the problem of continuing to find alternative drugs to antibiotics. Traditional Chinese medicine with antibacterial and anti-inflammatory functions is an ideal drug in the current environment of "reducing and replacing antibiotics" in the aquaculture industry. Therefore, it is urgent to develop traditional Chinese medicine prescriptions with good therapeutic effects and low cost. Summary of the Invention

[0005] To address the above problems, the present invention aims to provide a compound preparation of dried plum for treating chicken diarrhea and its preparation method.

[0006] The technical solution of the present invention is as follows:

[0007] On the one hand, a compound preparation of dried plum for treating chicken diarrhea is provided, the active ingredients of which are made from the following raw materials: dried plum, schisandra fruit, isatis root, and three needles, and the mass ratio of each raw material is 8-12:12-16:12-16:8-12.

[0008] Preferably, the mass ratio of the dried plum, schisandra fruit, isatis root, and three needles is 10:14:14:10.

[0009] Preferably, the dosage form of the ume-based antidiarrheal compound preparation is a liquid dosage form or a solid dosage form.

[0010] On the other hand, a method for preparing a liquid form of the above-mentioned compound preparation for treating chicken diarrhea using dried plum (Prunus mume) is also provided, comprising the following steps:

[0011] S1: Weigh each raw material according to the weight percentage and soak it in purified water for 45-120 minutes;

[0012] S2: Boil over a low flame for 45-90 minutes, then filter to obtain filtrate and residue;

[0013] S3: Add purified water to the filter residue again, simmer over low heat for 45-90 minutes, and then filter to obtain filtrate two;

[0014] S4: Mix the first filtrate with the second filtrate to obtain the liquid form of the Prunus mume antidiarrheal compound preparation.

[0015] Preferably, in step S1, purified water is added at a material-to-liquid ratio of 1:15 to 25.

[0016] Preferably, in step S3, purified water is added at a material-to-liquid ratio of 1:15 to 25.

[0017] The beneficial effects of this invention are:

[0018] (1) The present invention is inexpensive, with a formulation cost of about RMB 23 / kg, which is more than 50% lower than the price of commonly used compound preparations such as Pulsatilla chinensis oral liquid, Pulsatilla chinensis powder, Sihuang Zhili granules, and Siwei Chuanxinlian powder, and even more than 500% lower than the cost of Pulsatilla chinensis oral liquid / powder.

[0019] (2) After continuous use of the present invention for 4-5 days, the bacterial diarrhea rate (caused by Escherichia coli and Salmonella enteritidis) can be reduced from 80% to 0% on the 5th to 10th day, and the survival rate can be increased from 60% to 100%. The bacterial diarrhea cure rate of the present invention is 100%, while the cure rate of commercially available antidiarrheal compound prescriptions varies, ranging from 10% to 100%.

[0020] (3) On the 5th day after continuous use for 4 to 5 days, the present invention can completely inhibit the characteristic pathological changes of peritonitis, pericarditis, perihepatitis and enteritis caused by Escherichia coli and Salmonella pathogens.

[0021] (4) After continuous administration of the present invention for 5 days, the bacterial detection rate of diarrheal chickens can be reduced from 100% to 0%-20%.

[0022] (5) The present invention can restore the disordered intestinal flora and damaged intestinal villi of infected chickens.

[0023] (6) This invention can significantly reduce the inflammatory response in infected chickens. Attached Figure Description

[0024] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0025] Figure 1 The images show small intestinal tissue sections of an E. coli diarrhea model before and after treatment in a specific embodiment; where A and B are both E. coli challenge control groups, C is the treatment group of Example 1 of the present invention, and D is the healthy control group;

[0026] Figure 2 This is a schematic diagram showing the expression levels of intestinal inflammatory factors in chickens with Escherichia coli and Salmonella diarrhea after treatment with the compound preparation for stopping diarrhea of ​​Prunus mume in Example 1 of this invention, in a specific embodiment. Detailed Implementation

[0027] The present invention will be further described below with reference to the accompanying drawings and embodiments. It should be noted that, unless otherwise specified, the embodiments and technical features described in this application can be combined with each other. It should also be pointed out that, unless otherwise indicated, all technical and scientific terms used in this application have the same meaning as commonly understood by one of ordinary skill in the art to which this application pertains. The terms "comprising" or "including" and similar words used in this invention refer to elements or objects preceding the word that encompass the elements or objects listed following the word and their equivalents, without excluding other elements or objects.

[0028] On the one hand, the present invention provides a compound preparation of dried plum for treating chicken diarrhea, the active ingredients of which are made from the following raw materials: dried plum, schisandra, isatis root, and three needles, and the mass ratio of each raw material is 8-12:12-16:12-16:8-12.

[0029] In this invention, *Prunus mume* is the dried, nearly mature fruit of the plum tree (Prunus mume), a plant in the Rosaceae family. It has a sour and astringent taste and is neutral in nature. It enters the liver, spleen, lung, and large intestine meridians. It has the effects of astringing the lungs, astringing the intestines, promoting body fluid production, and expelling roundworms. It is used for chronic cough due to lung deficiency, chronic diarrhea and dysentery, thirst due to deficiency heat, and vomiting and abdominal pain due to roundworm infestation.

[0030] Schisandra chinensis is the dried, ripe fruit of the Schisandra chinensis plant, belonging to the Magnoliaceae family. It has a sour and sweet taste and is warm in nature. It enters the lung, heart, and kidney meridians. It has astringent and consolidating effects, replenishes qi and generates fluids, tonifies the kidneys and calms the mind. It is used for chronic cough and wheezing, nocturnal emission and spermatorrhea, enuresis and frequent urination, chronic diarrhea, spontaneous sweating and night sweats, thirst due to fluid depletion, internal heat and thirst, palpitations and insomnia.

[0031] Isatis root is the dried root of Isatis indigotica, a plant in the Brassicaceae family. It has a bitter taste and is cold in nature. It enters the heart and stomach meridians. It has the effects of clearing heat and detoxifying, cooling the blood, and relieving sore throat. It is mainly used to treat exogenous fever, early stages of febrile diseases, sore throat, febrile rashes, mumps, erysipelas, carbuncles, and boils.

[0032] Three-needle bark refers to the root bark or stem bark of plants in the Berberidaceae family, such as Berberis thunbergii, Berberis vulgaris, and Berberis thunbergii. It has a bitter taste and cold properties. It enters the liver, stomach, and large intestine meridians. It has the effects of clearing heat, promoting diuresis, and dispersing blood stasis. It is mainly used to treat dysentery, jaundice, sore throat, red eyes, and traumatic injuries.

[0033] By combining the efficacy and dosage of the above-mentioned raw materials, this invention can achieve 100% treatment of bacterial chicken diarrhea.

[0034] In one specific embodiment, the mass ratio of the dried plum, schisandra fruit, isatis root, and three needles in the compound preparation for stopping diarrhea of ​​the present invention is 10:14:14:10.

[0035] In one specific embodiment, the dosage form of the ume-based antidiarrheal compound preparation is either a liquid or a solid dosage form. It should be noted that dosage form refers to the form of a pharmaceutical preparation, a specific application form formed from processed pharmaceutical raw materials. By changing the physical form, it adapts to different treatment scenarios. The dosage form in this embodiment is merely a preferred dosage form of the present invention; other dosage forms also have similar therapeutic effects.

[0036] On the one hand, the present invention also provides a method for preparing a liquid form of the compound preparation for treating chicken diarrhea using dried plum, as described in any one of the above-mentioned methods, comprising the following steps:

[0037] S1: Weigh each raw material according to the weight parts and add purified water to soak for 45-120 minutes; optionally, add purified water at a material-to-liquid ratio of 1:15-25;

[0038] S2: Boil over a low flame for 45-90 minutes, then filter to obtain filtrate and residue;

[0039] S3: Add purified water to the filter residue again, simmer over low heat for 45-90 minutes, and then filter to obtain filtrate two; optionally, add purified water at a material-to-liquid ratio of 1:15-25;

[0040] S4: Mix the first filtrate with the second filtrate to obtain the liquid form of the Prunus mume antidiarrheal compound preparation.

[0041] It should be noted that the preparation method of the above embodiments is only a preferred liquid preparation method of the present invention. In the prior art, other methods such as ethanol soaking, reflux, filtration, concentration, etc. can also be applied to the present invention.

[0042] It should be noted that the preparation methods for other traditional Chinese medicine dosage forms, such as granules, are also existing technologies, and the specific steps will not be elaborated here.

[0043] Example 1

[0044] A compound liquid for treating chicken diarrhea using dried plum (Ume) is prepared through the following steps: Weigh 10g of dried plum, 14g of Schisandra chinensis, 14g of Isatis indigotica root, and 10g of Three-needle herb (i.e., the mass ratio of each raw material is 10:14:14:10), and soak the above four Chinese herbs in 1L of purified water for 1 hour. Then, simmer in a clay pot over low heat for 1 hour. Filter the first decoction and collect the filtrate. Then, add another 1L of purified water to the residue and simmer over low heat for 45 minutes. After simmering, filter the filtrate and mix it with the first decoction. Concentrate the mixture to 1g / mL to obtain the concentrated compound liquid for treating chicken diarrhea using dried plum.

[0045] In this embodiment, the obtained concentrated compound solution for treating diarrhea with dried plum is a brown liquid with a slightly bitter taste. Its functions are: astringent to stop diarrhea, clearing heat and drying dampness, and clearing heat and detoxifying. It is mainly used to treat bacterial diarrhea and stress-induced diarrhea in chickens. The dosage is 0.2 g / mL per chicken, administered orally for 5 consecutive days.

[0046] Example 2

[0047] Unlike Example 1, the mass ratio of dried plum, schisandra fruit, isatis root, and three needles in the prescription of this example is 12:12:12:12.

[0048] Example 3

[0049] Unlike Example 1, the mass ratio of dried plum, schisandra fruit, isatis root, and three needles in the prescription of this example is 8:16:16:8.

[0050] Example 4

[0051] Unlike Example 1, the purified water used in the first and second instances of this example is 0.8L each.

[0052] Example 5

[0053] Unlike Example 1, in this example, the drug solution was administered orally at a dosage of 0.1 g / mL / animal, and the feed was mixed with 10% of the recommended sensitive antibiotic dose, allowing free access to food.

[0054] Example 6

[0055] Unlike Example 1, in this example, the medicated solution is mixed with water at a concentration of 0.2 g / mL, and the chickens are allowed to eat freely. This method is suitable for 250 chickens.

[0056] Comparative Example 1

[0057] A compound antidiarrheal liquid for treating chicken diarrhea, which differs from Example 1 in that the prescription of this comparative example is made of dried plum, schisandra fruit, fraxinus bark, phellodendron bark, and kudzu root, with the mass ratio of each raw material being 10:10:14:14:10.

[0058] Comparative Example 2

[0059] A compound antidiarrheal liquid for treating chicken diarrhea, which differs from Example 1 in that the prescription of this comparative example is made of dried plum, schisandra fruit, fraxinus bark, phellodendron bark, and bitter orange peel, with the mass ratio of each raw material being 10:10:14:14:10.

[0060] Comparative Example 3

[0061] A compound antidiarrheal liquid for treating chicken diarrhea, which differs from Example 1 in that the prescription in this comparative example consists of dried plum, fraxinus bark, phellodendron bark, and rhubarb.

[0062] Comparative Example 4

[0063] A compound antidiarrheal liquid for treating chicken diarrhea, which differs from Example 1 in that the prescription of this comparative example is made of dried plum, schisandra fruit, sophora flavescens, andrographis paniculata, and kudzu root, with the mass ratio of each raw material being 10:10:14:14:10.

[0064] Comparative Example 5

[0065] A compound antidiarrheal liquid for treating chicken diarrhea, which differs from Example 1 in that the prescription of this comparative example is made of dried plum, isatis root, atractylodes macrocephala, dried ginger, and poria cocos, with the mass ratio of each raw material being 10:14:6:12:12.

[0066] Comparative Example 6

[0067] A compound antidiarrheal liquid for treating chicken diarrhea, which differs from Example 1 in that the prescription of this comparative example is made of dried plum, Atractylodes lancea, Magnolia officinalis, Citrus reticulata peel, and Andrographis paniculata, with the mass ratio of each raw material being 10:10:10:14:14.

[0068] Comparative Example 7

[0069] A compound antidiarrheal liquid for treating chicken diarrhea, which differs from Example 1 in that the prescription of this comparative example is made of dried plum, kudzu root, scutellaria baicalensis, isatis root, and andrographis paniculata, with the mass ratio of each raw material being 10:14:10:14:14.

[0070] Comparative Example 8

[0071] A compound antidiarrheal liquid for treating chicken diarrhea, which differs from Example 1 in that the prescription of this comparative example is made of dried plum, schisandra fruit, alisma rhizome, poria cocos, isatis root, three needles, and charred rhubarb, with the mass ratio of each raw material being 10:10:6:6:14:14:6.

[0072] Comparative Example 9

[0073] A compound antidiarrheal liquid for treating chicken diarrhea, which differs from Example 1 in that the prescription of this comparative example is made of dried plum, schisandra fruit, sophora flavescens, andrographis paniculata, kudzu root, three needles, and charred rhubarb, with the mass ratio of each raw material being 10:10:4:14:10:14:6.

[0074] Comparative Example 10

[0075] A compound antidiarrheal liquid for treating chicken diarrhea, which differs from Example 1 in that the prescription of this comparative example is made of dried plum, schisandra fruit, alisma rhizome, poria cocos, isatis root, and houttuynia cordata, with the mass ratio of each raw material being 10:10:6:6:14:14.

[0076] Comparative Example 11

[0077] A compound antidiarrheal liquid for treating chicken diarrhea, which differs from Example 1 in that the prescription of this comparative example is made of dried plum, schisandra fruit, andrographis paniculata, sophora flavescens, and kudzu root, with the mass ratio of each raw material being 10:10:14:14:10.

[0078] Based on the prices of medicinal materials as of July 12, 2025, as found on the Chinese Herbal Medicine World website, the prices of the raw materials used in this invention, the comparative examples, and other formulas in the prior art are shown in Table 1:

[0079] Table 1 Prices of various raw materials

[0080]

[0081]

[0082] As can be seen from Table 1, the single-herb medicine used in the Umeboshi Antidiarrheal Compound Liquid of this invention is inexpensive. Its price is reduced by more than 50% compared to commonly used compound liquids such as Pulsatilla chinensis oral liquid, Pulsatilla chinensis powder, Sihuang Zhili granules, and Siwei Chuanxinlian powder, and even by more than 500% compared to Pulsatilla chinensis oral liquid / powder.

[0083] Clinical application examples

[0084] The antidiarrheal compound solutions from each example and comparative example were concentrated to a concentration of 1 g / mL. Each chicken with diarrhea was administered 0.2 mL by gavage daily for 5 consecutive days. Data was measured and observed starting after the 5th day of treatment, and the observation period lasted for 10 consecutive days. The results are as follows: Figures 1-2 As shown in Tables 2 and 3:

[0085] Table 2. The therapeutic effects of various antidiarrheal compound solutions on Escherichia coli diarrhea.

[0086]

[0087]

[0088] Note: In Table 2, the diarrhea rate and survival rate are the results of days 5 to 10 of medication. The degree of characteristic pathological changes refers to the degree of characteristic pathological changes of peritonitis, pericarditis, perihepatitis and enteritis. The score is 0 for normal, 1 for mild inflammation and 2 for severe inflammation. The calculation method is the average of the sum of the degrees of peritonitis, pericarditis, perihepatitis and enteritis. The closer the score is to 2, the more severe the inflammation.

[0089] As shown in Table 2, chickens infected with E. coli in Examples 1 to 5, after continuous use of this invention, achieved 100% cure rate for diarrhea, 100% reduction in pathological changes caused by E. coli, and 90%–100% reduction in viral load in tissues and organs. Example 5 involved the combined use of this invention with a sensitive antibiotic; it can be seen that even with a 50% reduction in the dosage of this invention and a 90% reduction in the dosage of a sensitive antibiotic, a 100% cure rate was still achieved. In Example 6, the diarrhea rate and mortality rate were reduced by 75% after the medication was mixed with water and allowed free access to feed. A small number of chickens exhibited characteristic pathological changes, possibly because a small number of severely affected chickens did not drink enough water and did not receive sufficient medication. While chickens in Comparative Examples 1 to 11 showed varying degrees of therapeutic effect after oral administration of this invention, the results were still insufficient compared to those of this invention.

[0090] Table 3. The therapeutic effects of various antidiarrheal compound solutions on Salmonella enteritis diarrhea.

[0091]

[0092]

[0093] As can be seen from Table 3, after continuous use of the present invention, the Salmonella-infected chickens in Example 1 group can be cured of diarrhea by 100%, the pathological changes caused by Salmonella by 100%, and the viral load in tissues and organs by 80% to 90%. Compared with other comparative examples 1 to 11, the treatment effect is the most ideal.

[0094] from Figure 1As can be seen, the intestinal villi in the E. coli-infected group were disordered, with a small number of villi adhering (orange arrows) and indistinct boundaries. A very small number of goblet cells were distributed between the epithelial cells, and a small number of mucosal epithelial cells were edematous (blue arrows), with loose and pale cytoplasm. Numerous mucosal epithelial cells were sloughed off (black arrows), exposing the lamina propria. After treatment with the Umeboshi antidiarrheal compound solution of Example 1 of this invention, no significant pathological changes were observed between the epithelial cells of the chickens, similar to the healthy control group. This indicates that the treatment with this invention can protect the intestinal tissue of chickens from bacterial damage.

[0095] In addition, inflammatory factors were detected in the intestines of chickens with Escherichia coli and Salmonella diarrhea treated with the Umeboshi Antidiarrheal Compound Solution (this compound) of Example 1 of the present invention. The results are as follows: Figure 2 As shown. From Figure 2 It can be seen that the Ume Plum Antidiarrheal Compound Solution of the present invention can significantly reduce the severe inflammatory response caused by bacterial infection.

[0096] Furthermore, the *Prunus mume* antidiarrheal compound solution of Example 1 of this invention was used to clinically treat stress-induced diarrhea in chickens caused by sudden weather changes (administration method was the same as in Example 1). The results showed that chickens not treated with the formula of this invention still had a diarrhea rate of 50% and a survival rate of 80% after 5-10 days of observation. However, the diarrhea rate in chickens treated with the *Prunus mume* antidiarrheal compound solution of Example 1 of this invention decreased to 0%, and the survival rate was 100%. This demonstrates that the present invention also has ideal efficacy against stress-induced diarrhea caused by sudden weather changes.

[0097] In summary, the Umeboshi antidiarrheal compound liquid of the present invention is not only inexpensive, but also 100% effective in treating bacterial diarrhea, and has an ideal therapeutic effect on stress-induced diarrhea caused by sudden weather changes. Compared with the prior art, the present invention represents a significant advancement.

[0098] The above description is merely a preferred embodiment of the present invention and is not intended to limit the present invention in any way. Although the present invention has been disclosed above with reference to preferred embodiments, it is not intended to limit the present invention. Any person skilled in the art can make some modifications or alterations to the above-disclosed technical content to create equivalent embodiments without departing from the scope of the present invention. Any simple modifications, equivalent changes, and alterations made to the above embodiments based on the technical essence of the present invention without departing from the scope of the present invention shall still fall within the scope of the present invention.

Claims

1. A compound preparation of Fructus Mume for treating chicken diarrhea, characterized in that, The active ingredients are made of Fructus Mume, Schisandra Chinensis, Radix Isatidis and Radix Sophorae Flavescentis, and the mass ratio of each raw material is 8-12:12-16:12-16:8-12.

2. The plum astringent antidiarrheal compound preparation for treating chicken diarrhea according to claim 1, characterized in that, The mass ratio of the Fructus Mume, Schisandra Chinensis, Radix Isatidis and Radix Sophorae Flavescentis is 10:14:14:

10.

3. The plum astringent antidiarrheal compound preparation for treating chicken diarrhea according to claim 1, characterized in that, The dosage form of the Fructus Mume antidiarrheal compound preparation is liquid or solid.

4. The method of claim 1-3, wherein the liquid preparation of the Fructus Mume antidiarrheal compound preparation for treating chicken diarrhea is prepared by, The method comprises the following steps: S1: weigh each raw material and add purified water to soak for 45-120 min; S2: filter after decocting for 45-90 min to obtain filtrate 1 and residue; S3: add purified water to the residue again, and filter after simmering for 45-90 min to obtain filtrate 2; S4: mix the filtrate 1 and the filtrate 2 to obtain the liquid dosage of the Fructus Mume antidiarrheal compound preparation.

5. The preparation method of the liquid preparation of the Wumei antidiarrheal compound preparation for treating chicken diarrhea according to claim 4, characterized in that, In step S1, add purified water according to the solid-liquid ratio of 1:15-25.

6. The method for preparing the liquid form of the compound preparation for treating chicken diarrhea using dried plum as described in claim 4, characterized in that, In step S3, add purified water according to the solid-liquid ratio of 1:15-25.

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