A Chinese medicinal compound for treating bacterial diarrhea and its preparation method and application

Abstract

The present invention provides a Chinese medicine compound for treating bacterial diarrhea and a preparation method and application thereof, and relates to the technical field of traditional Chinese medicine. The Chinese medicine compound of the present invention is prepared by crushing, extracting, concentrating and other steps using coptis root, phellodendron, scutellaria, atractylodes, stir-fried astragalus, roasted liquorice and Shenqu as raw materials. The Chinese medicine compound of the present invention has a therapeutic effect on bacterial diarrhea, has the advantages of being easy to use, low treatment cost, reducing inflammation, high survival rate, rapid recovery of health and the like, and has no toxic side effects, and can be used clinically.

Description

Technical Field

[0001] The present invention relates to the technical field of traditional Chinese medicine, and in particular to a traditional Chinese medicine compound for treating bacterial diarrhea, and a preparation method and application thereof. Background Art

[0002] Bacterial diarrhea is one of the common digestive diseases in the aquaculture industry, and its incidence rate is relatively high in my country, which has had a serious impact on the production and development of my country's aquaculture industry. Among them, the disease caused by Escherichia coli is a highly contagious and high-mortality disease worldwide, which seriously affects the development of the aquaculture industry. Improper prevention and treatment will cause serious economic losses. Escherichia coli-induced diarrhea is one of the three major causes of death related to infectious diseases worldwide. Antibiotics are the first choice for Escherichia coli diarrhea, with the characteristics of rapid relief of symptoms, low cost and stable effect. In order to seek economic and production benefits, farmers use a large amount of antibiotics for treatment when livestock and poultry are sick, resulting in long-term irregular medication. Excessive use of antibiotics will bring a series of hidden dangers such as drug-resistant bacteria, mutant super bacteria, allergies, and flora disorders. The problem of bacterial resistance has become very serious. Therefore, it is particularly important to develop green drugs that can replace antibiotics. Summary of the invention

[0003] To solve the above problems, the present invention provides a Chinese medicine compound for treating bacterial diarrhea. The present invention defines the composition and ratio of raw materials and combines a specific preparation process to obtain a Chinese medicine compound with good therapeutic effect on bacterial diarrhea.

[0004] The Chinese medicinal compound for treating bacterial diarrhea of ​​the present invention comprises the following Chinese herbal medicines in parts by weight:

[0005] 5-15 parts by weight of Coptis chinensis, 6-18 parts by weight of Phellodendron amurense, 6-15 parts by weight of Scutellaria baicalensis, 8-20 parts by weight of Atractylodes lancea, 10-25 parts by weight of stir-fried Astragalus membranaceus, 4-10 parts by weight of roasted Licorice root, and 4-15 parts by weight of Shenqu.

[0006] Preferably, the Chinese medicinal compound for treating bacterial diarrhea is characterized by comprising the following Chinese herbal medicines in parts by weight:

[0007] 8-12 parts by weight of Coptis chinensis, 6-15 parts by weight of Phellodendron amurense, 8-15 parts by weight of Scutellaria baicalensis, 10-18 parts by weight of Atractylodes lancea, 12-20 parts by weight of stir-fried Astragalus, 5-8 parts by weight of roasted Licorice root, and 8-15 parts by weight of Shenqu.

[0008] Preferably, the Chinese medicinal compound for treating bacterial diarrhea is characterized by comprising the following Chinese herbal medicines in parts by weight:

[0009] 10 parts by weight of Coptis chinensis, 10 parts by weight of Phellodendron chinense, 10 parts by weight of Scutellaria baicalensis, 15 parts by weight of Atractylodes lancea, 15 parts by weight of stir-fried Astragalus membranaceus, 6 parts by weight of roasted Licorice root, and 10 parts by weight of Shenqu.

[0010] The present invention also provides a method for preparing the traditional Chinese medicine compound for treating bacterial diarrhea, comprising the following steps:

[0011] S1. Weigh coptis root, phellodendron, scutellaria, atractylodes, stir-fried astragalus, roasted liquorice and Shenqu according to proportion, crush them, and mix them evenly to obtain raw medicinal materials;

[0012] S2, mixing the raw medicinal materials with water, soaking them, heating and boiling them for extraction, filtering them after the extraction is completed, and repeatedly extracting the residue;

[0013] S3. The two extracts are combined and concentrated to obtain a Chinese herbal compound for treating bacterial diarrhea.

[0014] 5. The preparation method according to claim 4, characterized in that, in S1, the particle size of the raw medicinal material is ≤100 mesh.

[0015] Furthermore, in S2, the weight ratio of the raw medicinal material to water is 1:8.

[0016] Furthermore, in S2, the soaking time is 0.5-2h, and the extraction time is 0.5-2h.

[0017] Another object of the present invention is to provide the use of the Chinese medicine compound in treating bacterial diarrhea.

[0018] Furthermore, the treatment object is one or more of lambs, calves, and piglets.

[0019] Furthermore, the Chinese herbal compound can be prepared into any common dosage form for use, including but not limited to tablets, capsules, granules, aerosols, injections, films, etc.

[0020] In the present invention, Coptis chinensis is the rhizome of Coptis chinensis, Coptis triangularis or Coptis chinensis of the Ranunculaceae family, which is bitter and cold in nature and enters the heart, stomach, large intestine and liver meridians, and has the effects of clearing heat and dampness, purging fire and detoxifying; Phellodendron chinense is the bark of Phellodendron chinense of the Rutaceae family after the cork is removed, which is bitter and cold in nature and enters the kidney, bladder and large intestine meridians. It has the effects of clearing heat and dampness, purging fire and detoxifying; Scutellaria baicalensis is the root of Scutellaria baicalensis of the Labiatae family, which is bitter and cold in nature and enters the lung, stomach, gallbladder and large intestine meridians, and has the effects of clearing heat and dampness, purging fire and detoxifying; Atractylodes lancea is the rhizome of Atractylodes lancea or Atractylodes macrocephala of the Asteraceae family, which is bitter and warm in nature and enters the spleen and stomach meridians. It has the effects of strengthening the spleen and removing dampness, digesting food and relieving stagnation; fried astragalus is made from the rhizome of the leguminous plant astragalus or southwestern astragalus, dried ginger or salt water, is sweet and warm in nature, and enters the spleen and lung meridians, and has the effects of tonifying qi and strengthening the exterior, raising yang and restraining sweat; Shenqu is a kind of traditional Chinese medicine made by fermenting wheat as the main ingredient with yeast, etc., is sweet and warm in nature, and enters the spleen and stomach meridians, and has the effects of digesting food and relieving stagnation, and harmonizing the middle and stopping vomiting; roasted licorice is made from the root of the leguminous plant licorice or licorice glabra, processed with honey water, is sweet and flat in nature, and enters the heart, lung and spleen meridians, and has the effects of tonifying qi and harmonizing the middle, relieving acute pain and relieving pain.

[0021] The present invention not only complies with the principle of compatibility of monarch, minister, assistant and messenger in the field of traditional Chinese medicine, but also meets the requirements of drug efficacy evaluation and safety assessment in the field of modern medicine. Monarch, minister, assistant and messenger is an important principle of Chinese medicine prescription. It divides drugs into four categories: monarch, minister, assistant and messenger according to the role and status of the drugs in the prescription. Monarch drug is a drug that plays a major therapeutic role for the main disease or main syndrome, and is the core of the prescription; minister drug is a drug that assists the monarch drug to strengthen the therapeutic effect or plays a major therapeutic role for the concurrent disease or concurrent syndrome, and is the auxiliary of the prescription; adjuvant drug is a drug that reconciles the relationship between the monarch and minister, enhances or weakens its effect, or eliminates or alleviates its adverse reactions, and is the regulation of the prescription; messenger drug is a drug that guides other drugs to the diseased area or plays a special role, and is the medium of the prescription. According to this principle, the present invention selects Chinese medicinal materials with clear efficacy and mutual synergy to form a new type of Chinese medicine preparation. At the same time, the present invention also adopts scientific methods in the field of modern medicine to systematically study and evaluate the pharmacodynamic mechanism, effective ingredients, quality standards, clinical trials, etc. of the present invention, ensuring the effectiveness and safety of the present invention. Therefore, the present invention not only inherits the traditional wisdom of Chinese medicine, but also conforms to the development trend of modern medicine.

[0022] Compared with the prior art, the beneficial technical effects of the present invention are:

[0023] (1) The Chinese medicine compound preparation of the present invention has a therapeutic effect on bacterial diarrhea and has the advantages of being easy to use, low in treatment cost, reducing inflammation, having a high survival rate, and rapidly recovering health;

[0024] (2) The Chinese medicine compound preparation of the present invention has no toxic side effects and can be used clinically. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] The present invention will be further described below in conjunction with the accompanying drawings.

[0026] Figure 1 is the effect of the maximum dosage on the body weight of mice;

[0027] Figure 2 is the effect of the maximum dosage on the organ coefficient of mice;

[0028] Figure 3 Microscopic photos of the organ pathological histological examination of mice 14 days after administration (400×);

[0029] A: heart of control group, B: heart of maximum dosing group, C: liver of control group, D: liver of dosing group, E: spleen of control group, F: spleen of dosing group, G: lung of control group, H: lung of dosing group, I: kidney of control group, J: kidney of dosing group, K: stomach of control group, L: stomach of dosing group, M: intestine of control group, Q: intestine of dosing group;

[0030] Figure 4 The effect of Chinese herbal compound on the body weight of rats (n=10);

[0031] Figure 5 The effect of Chinese herbal compound on organ coefficients of rats (n=10);

[0032] Figure 6 These are tissue sections of the main organs of rats;

[0033] Figure 7 The effects of different treatments on the body weight of mice;

[0034] Figure 8 To investigate the effects of different treatments on inflammatory factors in mouse serum;

[0035] Fig. 9 The effect of the same treatment method on the spleen and thymus coefficients of mice;

[0036] Fig.10 To investigate the effects of different treatments on the intestines of mice;

[0037] Fig.11 The effects of different treatments on the jejunum, ileum and cecum of mice (400×). DETAILED DESCRIPTION

[0038] The technical solution provided by the present invention is further described below in conjunction with embodiments.

[0039] Example 1

[0040] A method for preparing a traditional Chinese medicine compound for treating bacterial diarrhea comprises the following steps:

[0041] S1. Weigh 10 parts by weight of Phellodendron chinense, 10 parts by weight of Scutellaria baicalensis, 15 parts by weight of Atractylodes lancea, 15 parts by weight of Astragalus membranaceus, 6 parts by weight of Radix Glycyrrhizae Preparata, and 10 parts by weight of Shenqu according to the proportion, crush them through a 100-mesh sieve, mix them evenly, and obtain the raw medicinal materials;

[0042] S2, mixing the raw medicinal materials with 8 times the weight of water and soaking for 2 hours, then heating and boiling to extract for 2 hours, filtering after the extraction is completed, and repeating the extraction once for the residue;

[0043] S3. The two extracts were combined and concentrated to obtain a Chinese herbal compound for treating bacterial diarrhea with a density of 1.6 g / mL.

[0044] Example 2 Acute toxicity test

[0045] The determination was carried out using the Chinese medicinal compound prepared in Example 1 as the raw material.

[0046] Median lethal dose (LD 50 ) determination:

[0047] 40 mice were randomly divided into 5 groups, 8 mice in each group, half of them were male and half were female. They were fasted for 12 hours before administration. The experimental group was gavaged at a dose ratio of 1:0.8. The doses of groups 1-4 were 16.0g / kg, 12.8g / kg, 10.2g / kg, and 8.2g / kg (calculated according to the original medicinal materials) by gavage, and the volume of drug solution gavage for each group of mice was 0.4mL / mouse. The control group was gavaged with an equal volume of normal saline. The observation was continued for 7 days, once in the morning and afternoon each day, and the animal toxicity and death were recorded in detail to calculate the LD 50 If there are dead mice, perform an autopsy on them; if the mice are not dead and the drug cannot be continued (LD 50 ), choose to do the maximum dosage test.

[0048] After oral administration, two mice in the 12.8g / kg group showed depression and rough fur after oral administration, but returned to normal after 6 hours. The rest of the mice were in normal mental state, moved freely, had smooth fur, and had normal defecation and urination, no abnormal secretions, and no adverse reactions. After continuous observation for 7 days, the spirit, appetite, drinking water, and activities of mice in each group were normal. After autopsy and pathological examination, no abnormal lesions were found in the main organs. From this experiment, it can be seen that the LD value of this drug cannot be obtained by the modified Koch method. 50 (LD 50 >16.0g / kg), according to the toxicological evaluation standard and drug toxicity classification standard, when LD 50 When the drug concentration is greater than 16.0 g / kg, it is considered non-toxic. Due to the limitation of the administration volume and drug concentration, it is difficult to determine the LD50. 50 Therefore, a maximum dosage test was conducted on mice.

[0049] Determination of maximum dosage:

[0050] 40 mice were divided into 2 groups, 20 mice in each group, half of them were male and half were female, and they were fasted for 12 hours (water was not allowed). The mice in the experimental group were gavaged with the Chinese medicine compound according to the maximum tolerance dose (maximum concentration 1.6g / mL, 0.8mL each time, twice within 24 hours), and the mice in the control group were gavaged with an equal volume of drinking water. The mice were fed routinely for 14 days after gavage, and the mental state of the mice was observed daily, and the poisoning symptoms, death time and number of mice were recorded. After the experiment, each group of mice was killed, and the blood indicators, organ coefficients and pathological changes of each organ of each group of mice were observed.

[0051] After the mice were gavaged with the Chinese medicine compound twice in the largest volume within 24 hours, the cumulative dose of the experimental group reached 128g / kg. After the gavage, the mice showed signs of lethargy and reduced activity due to gavage stress, and their activities returned to normal 4 hours later. After 14 days of observation, no deaths or abnormal conditions were observed. The changes in body weight of different experimental groups ( Figure 1 ) and blood indexes (Tables 1 and 2) were not statistically different from those in the control group; no major organ lesions were observed after autopsy, and the organ coefficient ( Figure 2 ) and tissue sections ( Figure 3 ) and the control group. The maximum dosage of the Chinese medicine compound is 128g / kg.

[0052] Table 1 Changes in blood routine of mice in each group (n=10)

[0053]

[0054]

[0055] Table 2 Changes in blood biochemistry of mice in each group (n=10)

[0056]

[0057] Subchronic toxicity test:

[0058] According to the results of the acute toxicity test of the Chinese medicine compound and its clinical dosage, the high, medium and low doses of subchronic toxicity were determined to be 16g / kg, 8g / kg and 4g / kg respectively. 80 rats were taken, half male and half female, and raised separately. After 7 days of pre-feeding, they were randomly divided into 4 groups, namely high, medium and low dose groups of the drug and a blank control group, with 20 rats in each group. Using the equal volume and different concentration method, the rats in each test group were gavaged and the control group was given an equal amount of normal saline. The drug was administered once a day for 30 consecutive days.

[0059] On the second day after administration, three rats in the medium-dose group showed signs of huddling, ragged fur, and lethargy, and one rat showed signs of discomfort such as loose stools. These symptoms returned to normal on the third day, and no rats died during the experiment. Figure 4 It can be seen that after 30 days of intragastric administration, there was no significant difference in body weight between the three Chinese medicine compound dosage groups and the control group (P>0.05), indicating that the Chinese medicine compound had no significant effect on the growth and development of rats.

[0060] Changes in hematological indicators:

[0061] As can be seen from Table 3, compared with the control group, the hemoglobin in the high-dose group (P < 0.05) and the average platelet volume in the low-dose group were increased (P < 0.05), and there were no statistically significant differences in the other indicators between each dose group and the control group (P > 0.05).

[0062] Table 3 Changes in blood routine of rats in each group (n=10)

[0063]

[0064]

[0065] Changes in blood biochemical indicators:

[0066] Compared with the control group, the alanine aminotransferase (ALT) content of rats in the low-dose group was decreased (P < 0.05), and there were no statistically significant differences in other indicators between each dose group and the control group (P > 0.05) (Table 3-4).

[0067] Table 4 Changes in blood biochemistry of rats in each group (n=10)

[0068]

[0069] Organ index:

[0070] The results show that Figure 5 ), after intragastric administration, the spleen coefficient of the male high-dose group was significantly higher than that of the control group, and there were no significant differences in the other organ indices compared with the control group (P>0.05).

[0071] Pathological changes in autopsy:

[0072] After intragastric administration, the rats were autopsied and the size and color of the main organs were the same as those of the control group. No pathological changes such as bleeding spots, swelling, and necrosis were observed. The pathological sections of the rat tissues were observed under an optical microscope, and a small amount of red blood cells were found in the kidneys of some rats in the high-dose group. There were no significant differences in other organs between the high-dose group and the control group ( Figure 6 ).

[0073] Example 2

[0074] The Chinese medicinal compound prepared in Example 1 was used as a raw material to measure the therapeutic effect of bacterial diarrhea.

[0075] The Escherichia coli diarrhea model was established using the following method:

[0076] Streptomycin (5 g / L) was added to the drinking water of mice 48-12 h before inoculation with E. coli suspension to eradicate the normal resident flora in the intestine. The streptomycin-treated water also contained fructose (6.7 wt%) to encourage water consumption before inoculation with E. coli suspension. 12 h before inoculation with E. coli suspension, food was removed and the fructose-treated water was replaced with unsweetened sterile water. After fasting for 12 h, all mice were supplemented with 0.2 mL of 0.3% NaHCO3 aqueous solution. After 30 min, different groups of mice were gavaged with the prepared 6×108 cfu / mL E. coli suspension in the morning of 3-5 days, and the mice were gavaged with 0.2 mL / 10 g·d by oral gavage.

[0077] Grouping and processing methods:

[0078] 48 Kunming mice were randomly divided into a normal group, a model group, a Chinese medicine treatment group, and a positive control group (enrofloxacin). Except for the normal group, the remaining 36 mice were modeled according to the above method. After the model was successfully established, drug treatment began:

[0079] Normal group: administered with an equal volume of normal saline;

[0080] Model group: administered with an equal volume of normal saline;

[0081] Chinese medicine treatment group: oral administration of Chinese medicine compound 4.12g / (kg·d);

[0082] Enrofloxacin: Oral administration of enrofloxacin 5 mg / (kg·d).

[0083] Measurement of weight change and diarrhea index:

[0084] During the oral administration of mice, the weight of mice was weighed at regular intervals every day and the changes were recorded. At regular intervals every day, mice were placed in cages with filter paper to observe the diarrhea of ​​mice (mouse feces were divided into 5 types: normal feces; feces with normal appearance but high water content; unformed soft feces; watery feces; feces containing mucus or mucus, pus and blood). The first two were normal, and the last three were considered diarrhea. The number of defecation, the number of abnormal feces, and the level of loose stools in each cage of mice within 6 hours were recorded, and the average loose stool rate, loose stool level, and diarrhea index of each cage of mice were calculated based on the above recorded data.

[0085] After the model group mice were given E. coli suspension, they developed rough fur, arched back, mental fatigue, decreased appetite, chills, reduced activity, weight loss, diarrhea, etc. After the positive control group was treated with enrofloxacin, the weight of the mice increased. After the Chinese medicine group was treated with the drug, the weight recovery was more significant, which was statistically significant (p < 0.05). The specific weight changes of the experimental mice in each group are as follows Figure 7 shown.

[0086] Loose stool grade: An indicator of the severity of unformed soft stools or watery stools or mucous stools. The size of the stain area formed by the contaminated filter paper is divided into four grades. The diameter of the grade 1 stain is less than 1 cm, the diameter of the grade 2 stain is 1-2 cm, the diameter of the grade 3 stain is 2-3 cm, and the diameter of the grade 4 stain is more than 3 cm. The diameter of a circular stain with a more regular shape can be measured directly, and the diameter of the longest and approximate circle of the irregular stain can be measured. The sum of the two divided by the sum represents the approximate diameter of the irregular stain. In statistics, the grade of each loose stool is measured one by one, and then all the loose stool grades of each mouse in each cage are added one by one, and divided by the number of loose stools to obtain the average loose stool grade number per mouse.

[0087] Average loose stool grade = total loose stool grade ÷ total loose stool frequency.

[0088] Loose stool rate (%) = (number of loose stools per mouse ÷ total number of defecations per mouse) × 100%.

[0089] Diarrhea index = loose stool rate × average loose stool grade.

[0090] The diarrhea index of mice in all groups is shown in Table 5. After modeling, the diarrhea index of all E. coli infection groups increased compared with the normal group (P < 0.05). Compared with the model group, the diarrhea index of all drug treatment groups was significantly reduced (P < 0.05). Compared with other treatment groups, the treatment time of the Chinese medicine + FMT group and the positive control group was short.

[0091] Table 5 Effects of different treatments on diarrhea index caused by Escherichia coli n = 12

[0092] Day 1 the next day Day 3 Day 4 Day 5 Control group 0a 0a 0a 0a 0a Model Group 1.69±0.27b 1.28±0.20b 0.46±0.08b 0.11±0.04a 0a Chinese Medicine Group 0.98±0.18d 0.14±0.11a 0.04±0.01a 0a 0a Enrofloxacin group 0.73±0.18c 0.12±0.10a 0a 0a 0a

[0093] Note: Data in the same column with completely different lowercase letters indicate significant differences (P < 0.05), while data with the same lowercase letters indicate insignificant differences (P > 0.05).

[0094] Detection of inflammatory factors:

[0095] After gavage on the 5th day of the experiment, whole blood was collected and blood was collected using EDTA-free blood collection tubes, placed in an ice box for an appropriate period of time, and centrifuged at 3000 rpm for 1 min. Serum from each group was collected and marked, and placed in a -20°C refrigerator to determine the levels of cytokines (IL-1β, IL-6, TNF-α) in serum using ELISA.

[0096] like Figure 8 As shown, the levels of IL-6, TNF-α, and IL-1β in the blood of mice in the positive control group treated with enrofloxacin were significantly lower than those in the modeling group, with significant statistical differences (p<0.01); the levels of IL-6, TNF-α, and IL-1β in the blood of mice in the Chinese medicine group were significantly lower than those in the modeling group, with significant statistical differences (p<0.05).

[0097] Determination of spleen and thymus coefficients:

[0098] After the intragastric administration on the 5th day of the experiment, the mice were anesthetized with ether and killed by cervical dislocation. The spleen and thymus were collected and marked, washed once with saline, and the surface moisture was dried with filter paper. The organ coefficient was calculated using the following formula:

[0099] Organ coefficient (%) = (organ weight (g) / body weight (g)) × 100%.

[0100] Fig. 9 The results of spleen and thymus index of mice in each group are shown. Compared with the normal group, the spleen and thymus index of mice in the model group increased significantly (P < 0.01). Compared with the model group, the spleen index of the Chinese medicine treatment group decreased significantly (P < 0.01).

[0101] Pathological histological examination:

[0102] The organs were observed with the naked eye, and the intestinal tissue of the mice was taken, fixed with 4% neutral formalin fixative, embedded, and observed under a microscope after HE staining.

[0103] The mice in each group were autopsied. The infected group had mucus in the chest and abdominal cavities, intestinal adhesions, significantly shortened intestines, swollen cecum, and the intestines were filled with yellow liquid. The feces in the rectum were not formed. After 5 days of drug treatment, the enrofloxacin treatment group recovered the most. There was no significant difference in intestinal length between the control group and the control group. The cecum was not swollen, there was no yellow liquid in the intestine, and the feces in the rectum were formed. The intestines of the Chinese medicine treatment group also recovered ( Fig.10 ), the results of intestinal pathological histology of the mouse model are shown in Fig.11 .

[0104] Fig.10The results showed that in the modeling group, the intestinal mucosal epithelial cells showed necrosis and sloughing, the intestinal villi were broken and shed in large numbers, the goblet cells proliferated in large numbers, and there was a large amount of inflammatory cell infiltration. In the enrofloxacin treatment group, the number of inflammatory cells decreased, and the damaged tissue began to show varying degrees of repair and regeneration. Although the inflammatory cell infiltration decreased in the traditional Chinese medicine treatment group, goblet cells still existed and the intestinal villi still shed, and the difference was not significant.

[0105] The principles and implementation methods of the present invention are described in this article using specific examples. The description of the above embodiments is only used to help understand the method and core idea of ​​the present invention. At the same time, for those skilled in the art, according to the idea of ​​the present invention, there will be changes in the specific implementation methods and application scope. In summary, the content of this specification should not be understood as limiting the present invention.

Claims

1. A Chinese medicinal compound for treating Escherichia coli diarrhea, characterized in that: It is composed of the following Chinese herbal medicines in parts by weight: 10 parts by weight of Coptis chinensis, 10 parts by weight of Phellodendron chinense, 10 parts by weight of Scutellaria baicalensis, 15 parts by weight of Atractylodes lancea, 15 parts by weight of stir-fried Astragalus membranaceus, 6 parts by weight of roasted Licorice root, and 10 parts by weight of Shenqu.

2. A method for preparing the Chinese medicinal compound for treating Escherichia coli diarrhea according to claim 1, characterized in that: The following steps are involved: S1. Weigh coptis root, phellodendron, scutellaria, atractylodes, stir-fried astragalus, roasted liquorice and Shenqu according to proportion, crush them, and mix them evenly to obtain raw medicinal materials; S2, mixing the raw medicinal materials with water, soaking them, heating and boiling them for extraction, filtering them after the extraction is completed, and repeatedly extracting the residue; S3. The two extracts are combined and concentrated to obtain a Chinese herbal compound for treating Escherichia coli diarrhea.

3. The preparation method according to claim 2, characterized in that: In S1, the particle size of the raw medicinal material is ≤100 mesh.

4. The preparation method according to claim 2, characterized in that: In S2, the weight ratio of the raw medicinal material to water is 1:

8.

5. The preparation method according to claim 2, characterized in that: In S2, the soaking time is 0.5-2 h, and the extraction time is 0.5-2 h.

6. The preparation method according to claim 2, characterized in that: In the S3, the density of the concentrated Chinese herbal compound mixture is 1.6 g / mL.

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