45 results about "Salmonella Bacteriophage" patented technology

The invention relates to a Salmonella bacteriophage, particularly a wide-host-spectrum strongly-lytic Salmonella pullorum bacteriophage. The invention also relates to Salmonella pullorum for controlling the bacteriophage in a culture environment. The Salmonella pullorum bacteriophage is named PSPu-4-116, and collected in China Center for Type Culture Collection, and the collection number is CCTCC M2011042.

The invention discloses a salmonella phage LPSE34. The preservation number of the salmonella phage LPSE34 is CCTCC NO 2018121. The phage is a broad-spectrum salmonella phage and can crack drug-tolerant salmonellas, it is identified that the phage belongs to myoviridae, and the phage is named as LPSE34. The phage LPSE34 is stable in titer at the pH of 4-12 and the temperature of 30-60 DEG C. The invention further discloses application of the salmonella phage in food, especially in chicken and ham systems; and in addition, the invention further discloses a compound edible coating film formed bytaking the phage as an antibacterial substance to be combined with sodium alginate, and compared with antibiotics and chemical preservatives, the phage cannot affect the texture and flavor of food andhas the characteristics of high specificity, no residue and safety. The salmonella phage LPSE34 can serve as a bacteriocidal substance/the antibacterial substance to control pollution of food-borne pathogens to the food and is a very promising biological agent for guaranteeing the food safety.

The invention belongs to the technical field of biology and particularly relates to endolysin sourced from salmonella bacteriophage and an application thereof. The amino acid sequence of the endolysin sourced from the salmonella bacteriophage includes the total of the partial of the amino acid sequence represented as the SEQ ID No.1. Sequence structure analysis proves that the protease is a lyase which can lysing bacterial cell walls in the bacteriophage. A test proves that the protein formed by the amino acid sequence is good in bactericidal activity. The endolysin, when being expressed and purified by a suitable expression vector on market, can achieve a significant sterilization effect, so that the endolysin can be used for preparing a bacterial inhibitor of salmonella.

The invention discloses a salmonella bacteriophage and an application thereof in medicines for preventing and treating salmonella infection diseases. The bacteriophage is named as SPP11 and is preserved in China General Microbiological Culture Collection Center, wherein the preservation date is Nov.04,2019, and the preservation number is CGMCC No.18868. The bacteriophage SPP11 with a strong cracking spectrum has a strong cracking effect on salmonella, can effectively prevent and control salmonella diseases in poultry farms, reduce positivity of the pullorum disease antibody of chickens, reducediarrhea of chickens caused by salmonella enteritis, and can also be used for salmonella sterilization of feeding environment, feed, drinking water and the like. In addition, the bacteriophage is easy for industrial production, and medicines or disinfectants prepared by the bacteriophage not only have reduced cost, but also have the advantages of environmental friendliness.

The present invention relates to novel bacteriophages and bacteriophage cocktails containing the novel bacteriophages and or parts and/or products of them, all of them belonging to the Caudovirales order. The novel bacteriophages and the bacteriophage cocktails containing the novel bacteriophages and or parts and/or products of them are Salmonella specific bacteriophages and have lytic activity against Salmonella.

The invention discloses a salmonella bacteriophage and bacteriophage anti-bacterium composition and an application thereof. A bacteriophage is salmonella enteritidis bacteriophage LPSE1 and salmonella typhimurium bacteriophage LPST10, and both the salmonella enteritidis bacteriophage LPSE1 and the salmonella typhimurium bacteriophage LPST10 have excellent inhibiting effect on salmonellas and good tolerance for pH (potential of hydrogen) and temperature. Application examples of the salmonella bacteriophage to food systems verify that the bacteriophage has excellent effect when being used for bacteriostatic agents. The salmonella enteritidis bacteriophage LPSE1 and the salmonella typhimurium bacteriophage LPST10 have synergistic effects after being mixed and have more beneficial inhibiting effect as compared with a single bacteriophage.

The present invention is directed to isolated Salmonella bacteriophage, and methods of using Salmonella bacteriophage, or polynucleotides and polypeptides derived therefrom, to control the growth or contamination of food products by Salmonella. The present invention also contemplates the use of Salmonella bacteriophage, and polynucleotides and polypeptides derived therefrom, for the treatment of host infections or environmental contamination by Salmonella.

The invention provides a bacteriophage composition, a bacteriostatic agent and application thereof, and belongs to the technical field of bioengineering. The bacteriophage composition comprises a clostridium perfringens bacteriophage (CCTCC NO: M2020047), a salmonella bacteriophage (CCTCC NO: M2020710), an escherichia coli bacteriophage (CCTCC NO: M2020711) and a shigella flexneri bacteriophage (CCTCC NO: M209029). The bacteriophage composition has a strong lysis effect on clostridium perfringens, salmonella, escherichia coli and shigella flexneri, and can be used for inhibiting and/or eliminating clostridium perfringens, salmonella, escherichia coli and shigella flexneri; by inhibiting the overgrowth of clostridium perfringens, salmonella, escherichia coli and shigella flexneri, the occurrence of intestinal diseases of animals is effectively prevented and controlled; a safe bacteriophage antibacterial product source without toxic and side effects is provided for preventing and controlling animal intestinal diseases caused by clostridium perfringens, salmonella, escherichia coli and shigella flexneri.

The invention discloses a salmonella bacteriophage nano magnetic bead conjugate and its enrichment and separation kit. The conjugate is a complex of a salmonella bacteriophage coupled with a carboxylmagnetic bead, wherein the salmonella bacteriophage is Salmonella Typhimurium bacteriophage LPST10, and a preservation number is CCTCC NO: M 2016473. The bacteriophage of the Salmonella bacteriophagenano magnetic bead conjugate of the invention can specifically recognize and infect bacteria, and has the characteristics of good specificity and small molecular weight. The kit is capable of rapidlyand sensitively separating and enriching salmonella.

The invention belongs to the technical field of salmonella phages, and particularly relates to an acid-resistant phage isolate capable of specifically cracking salmonella and application of the acid-resistant phage isolate in sterilization and bacterium prevention. The invention mainly discloses salmonella pullorum phage SG4P1 with the preservation number of CCTCC NO: M2018765. The salmonella phage is a strong phage isolated from the nature, and toxicological experiments prove that the salmonella phage is safe and free of any side effect. The experiments find that the phage can tolerate extremely high acidity, and therefore, the salmonella phage can have a strong cracking effect on salmonella in the intestinal tract through gastric acid. The phage is expected to be developed into a biological bactericide for preventing and treating infection and pollution of the salmonella.

The invention provides a preparation method of novel salmonella bacteriophage microencapsulated microspheres. The method comprises the following steps of mixing a cationic etherified starch solution and a bacteriophage suspension, and uniformly stirring to obtain a first mixed solution; uniformly stirring and mixing the first mixed solution, a sodium alginate solution, a xanthan gum solution and a nano TiO2 solution to obtain a second mixed solution; and dropwise adding the second mixed solution into a calcium chloride solution to form calcium alginate gel, filtering and collecting bacteriophage microspheres, putting the bacteriophage microspheres into a cationic chitosan oligosaccharide solution, coating for 30 minutes, and filtering and collecting the bacteriophage microencapsulated microspheres. In actual use, after the bacteriophage microspheres enter gastric juice, the bacteriophage surface coated material can effectively resist erosion of gastric acid and prolong the survival time of bacteriophage, and after the bacteriophage microspheres enter intestinal juice, the bacteriophage surface coated material can react with the intestinal juice and is cracked to release the bacteriophage in the material so as to achieve the sterilization effect. The method has the advantages that microencapsulation preparation is carried out at room temperature, the process is simple, the application range is wide, and the cost is low; and the raw materials are wide in source, cheap and easy to obtain, and the bacteriophage in the product is high in activity and encapsulation efficiency and can be directly applied to feed addition.

The invention discloses a salmonella bacteriophage with a wide lysis spectrum and high temperature resistance. The preservation number is CGMCC No.21995. After whole genes are sequenced, the salmonella bacteriophage does not have virulence genes and lyogenic genes. The salmonella bacteriophage RDP-SA-20049 acts for 60 min at the high temperature of 90 DEG C, the titer is kept at 1.0 * 10 < 7 > pfu/mL or above, and the salmonella bacteriophage RDP-SA-20049 has high biological activity and good high temperature resistance. The salmonella bacteriophage can be used for lysing pathogenic bacteria including 5 strains of salmonella typhimurium, 4 strains of salmonella pullorum, 1 strain of salmonella paratyphi B, 1 strain of salmonella typhimurium, 7 strains of salmonella enteritidis and salmonella BS-20036, and has the characteristic of high cracking rate, the cracking rate is up to 99%, the cracking performance is stronger, and the salmonella bacteriophage has good advantages in treatment of salmonella infection diseases, and is high in titer and good in prevention and treatment effect.

The invention discloses a preparation method and a test method of a bacteriophage mixed preparation for killing salmonella, and relates to the technical field of veterinary sterilization preparations.The preparation method disclosed by the invention comprises the following steps: s01, performing induction and identification of mild salmonella bacteriophage; s02, purifying the salmonella bacteriophage; s03, storing the suspension; and s04, preparing phage cocktail; the test method comprises the following steps: t1, preparing a liquid culture medium; t2, diluting a bacterial liquid; t3, performing mixing preparation; and t4, performing reference test. For the salmonella with different concentrations, the preparation has a good bactericidal effect, can improve the bactericidal ability of thesalmonella compared with antibiotics and reduce the generation of bacterial resistance, and is a mixed preparation applied to environmental sterilization instead of the antibiotics.

The invention discloses a bacteriophage composition and an application of the bacteriophage composition in a medicine for preventing and treating chicken salpingitis. The bacteriophage composition comprises a coliphage PD07 and a salmonella bacteriophage PYC04. A preservation number of the coliphage PD07 is CGMCC No18865, and the coliphage PD07 is preserved in CGMCC on November 4, 2019; the preservation number of the salmonella bacteriophage PYC04 is CGMCC No18867, and the salmonella bacteriophage PYC04 is preserved in the CGMCC on November 4, 2019. According to the bacteriophage composition,the coliphage PD07 and the salmonella bacteriophage PYC04 are compounded to be used as a component for effectively preventing and treating the chicken salpingitis; and compared with a single lytic escherichia coli phage, the bacteriophage composition has advantages that a sterilization range is greatly widened; sterilization activity is improved; a laying rate of laying hens can be increased, andphysique and disease resistance of the laying hens are enhanced.

The invention discloses a salmonella bacteriophage RDP-SA-21003 capable of simultaneously splitting escherichia coli, and the preservation number is CGMCC No. 22494. After whole genes are sequenced, the salmonella bacteriophage RDP-SA-21003 does not have virulence genes and lyogenic genes; the cracking efficiency of the obtained bacteriophage on host bacteria BS20078 is 98.7% or above, when the bacteriophage is independently used, the cracking capacity on salmonella is relatively high, and the cracking spectrum is wide; and the bacteriophage RDP-SA-21003 has the capability of cracking escherichia coli causing chicken air sacculitis and peritonitis.

The invention belongs to the technical field of animal infectious disease prevention and treatment, and particularly relates to a broad-spectrum high-temperature-resistant salmonella virulent bacteriophage and application thereof. The salmonella bacteriophage is preserved in the China Center for Type Culture Collection, and the preservation number is CCTCC NO: M20211353. The salmonella bacteriophage has a good killing effect on salmonella drug-resistant strains, also has a good treatment effect on salmonella infection, is suitable for being used as a biological agent for preventing and controlling salmonella, and can be applied to comprehensive prevention and control of salmonella.

The invention discloses a salmonella bacteriophage resistant to antiviral drugs. The preservation number of the salmonella bacteriophage is CGMCC No.21994. After sequencing is carried out on whole genes, the salmonella bacteriophage does not have virulence genes and lyogenic genes. After the salmonella bacteriophage RDP-SA-20032 is treated for 60 minutes at 60 DEG C, the titer is still kept at 10<9> pfu/mL, and the salmonella bacteriophage RDP-SA-20032 has better tolerance to temperature; the titer is still kept at 10<11>pfu/mL when the bacteriophage is cultured for 1 hour at 37 DEG C in a pH range of 4-9, and the bacteriophage still survives when the pH is 12 or 2, so that the acid-base tolerance range of the bacteriophage is relatively wide; the salmonella bacteriophage has good titer stability under the coexistence condition of the salmonella bacteriophage and antiviral drugs; and after being redissolved, the phage spray-dried powder is still stable in titer after being kept at 4 DEG C for 72 hours, can tolerate higher temperature, and can be produced by adopting a conventional spray drying tower, the production efficiency is improved, and the production cost is reduced.

The invention discloses a salmonella bacteriophage CKT1 without transduction of drug-resistant gene and application of the salmonella bacteriophage CKT1, the virulent bacteriophage CKT1 is preserved in China Center for Type Culture Collection on January 21, 2022, and the preservation number of the virulent bacteriophage CKT1 is CCTCC (China Center for Type Culture Collection) 2022110. The separated salmonella bacteriophage CKT1 has splitting activity on D-group salmonella such as salmonella pullorum, salmonella gallinarum and salmonella enteritidis from different sources, is short in incubation period, high in progeny bacteriophage outbreak amount and low in bacteriophage mutation resistance rate, does not carry virulence genes and drug-resistant genes on a genome, does not have transduction capability, and can be used for preparing the salmonella bacteriophage CKT1. The bacteriophage does not cause transmission of drug-resistant genes, is a safe, efficient and high-specificity virulent bacteriophage, and does not destroy intestinal flora of chicks. The bacteriophage preparation can become a novel environment-friendly auxiliary means for purifying salmonella pullorum in a breeding chicken farm, and is also an alternative strategy for preventing and treating salmonella pullorum or salmonella gallinarum infection of chicken flocks.

The invention relates to the field of phage technology, in particular to a Salmonella phage with high temperature resistance and wide cracking spectrum and a composition thereof. Described Salmonella phage is Salmonella phage SG8P3 ( Salmonella pullorum Phage SG8P3). The Salmonella bacteriophage SG8P3 of the present invention has good high temperature resistance characteristics, 65 ℃ of water baths 2h still have 80% survival rate, 75 ℃ of lower water baths 2h have 10 ⁶ PFU/mL titer. At a concentration of 10 ² ~10 ³ cfu/mL Salmonella medium, 10 ⁴ ~10 ⁶ PFU/mL of Salmonella phage SG8P3 can sterilize more than 99% of the above-mentioned concentrations of Salmonella, and has a broad-spectrum bactericidal ability against Salmonella; the composition of Salmonella phage SG8P3 can crack more Salmonella, and the cleavage rate can reach more than 98%, with Stronger disintegration.

The invention provides a salmonella phage composition and application thereof, and belongs to the technical field of biology. The salmonella bacteriophage composition provided by the invention comprises at least one of a bacteriophage GSP162, a bacteriophage GSP193, a bacteriophage GSP001 and a bacteriophage GSP032. The salmonella bacteriophage composition provided by the invention plays a role in four receptors of salmonella lipopolysaccharide (LPS) O antigen, LPS core polysaccharide, outer membrane protein BtuB and outer membrane protein TolC respectively. The four bacteriophages screened by the invention have good temperature tolerance and acid-base tolerance, can be used for cracking salmonella of various different serotypes, and are wide in cracking spectrum; an in-vitro bacterial lysis experiment shows that compared with the use of a single phage, the phage cocktail can effectively delay the appearance of an anti-phage strain, and the synergistic effect is obvious. A mouse intestinal sterilization test shows that the bacteriophage composition disclosed by the invention can be used for effectively reducing the salmonella carrying amount in cecum.