64 results about "Paratyphoid fever" patented technology

The invention relates to a double coptis for injection as a veterinary herbal medicine compound preparation and a preparation technique as well as an application thereof. The compound preparation is made from the following parts of medicines by weight: 1250 portions to 3750 portions of honeysuckle, 1250 portions to 3750 portions of scutellaria and 2500 portions to 7500 portions of forsythia. Sustained release accessories contain 200 portions of hydroxypropyl methylcellulose and 100 portions of sodium carboxymethylcellulose. The containing of sustained release accessories can reduce injection times for large animals and the stress response of large animals. The veterinary herbal injection preparation has the functions of antiviral, antibacterial, anti-inflammatory and antipyretic analgesic effects, as well as improving immunity and the like, and can be applied to epidemic diarrhea, transmissible gastroenteritis, swine paratyphoid, septicemia caused by various diseases, cough, dyspnea, skin cyanosis, arthrocele, lameness, swine reproduction and respiratory disturbance syndrome and cow mastitis caused by streptococcosis, haemophilus parasuis infection, contagious pleuropneumonia, swine lung plague in veterinarian clinical practice.

The invention discloses a typhoid fever and paratyphoid fever combined vaccine. The typhoid fever and paratyphoid fever combined vaccine comprises typhoid Vi polysaccharide and paratyphoid fever thallus specific polysaccharide O-SP. The invention further discloses a preparation method of the vaccine. The preparation method comprises the steps of preparing the typhoid Vi polysaccharide, preparing the paratyphoid fever thallus specific polysaccharide O-SP, preparing typhoid Vi polysaccharide-carrier protein conjugate, preparing paratyphoid fever thallus specific polysaccharide O-SP-carrier protein conjugate and preparing end products. Animal experiments prove that the typhoid fever and paratyphoid fever combined vaccine can induce high-level anti-typhoid Vi polysaccharide and anti-paratyphoid fever lipopolysaccharide (LPS) antibody, has immunologic memory reaction, and has no acute toxicity reactions.

The present invention is drawn to a live, attenuated S. Paratyphi A strain, a live, attenuated S. Paratyphi A strain comprising a stabilized plasmid expression system, an S. Paratyphi conjugate vaccine, and methods of using these strains and conjugate vaccine.

The invention discloses antibacterial maca spray. The antibacterial maca spray comprises, by weight, 0.5-1.0% of maca extract liquid, 1.0-1.5% of cinnamon extracts, 0.1-0.15% of clove extract liquid, 0.1-0.15% of capsicum annuum extract liquid, 0.1-0.5% of cistanche deserticola extracts, 0.3-0.5% of chlorhexidine acetate and the balance water. The antibacterial maca spray has the advantages that inhibition effects can be realized for hemolytic streptococcus, streptococcus pneumoniae, Escherichia coli bacteria, proteus vulgaris, salmonella paratyphi A, shigella dysenteriae, Escherichia coli, staphylococcus aureus, methicillin-resistant staphylococcus aureus, candida albicans, pseudomonas aeruginosa and the like, the antibacterial maca spray can be used for inhibiting the Escherichia coli, the staphylococcus aureus, the candida albicans and the like and has little skin irritation, and good wetting effects can be realized.

The invention discloses a traditional Chinese drug composition for pig and a preparation method and application thereof. The composition comprises drug substances of 6-18 parts of indigofera tinctoria by weight, 4-12 parts of Chinese amphibious knotweed rhizome by weight and 5-15 parts of Chinese mosla herb by weight. The preparation method of the composition comprises: distilling Chinese mosla herb, and collecting the distillate; adding indigofera tinctoria and Chinese amphibious knotweed rhizome in the residue and residual liquid after distillation and decocting for 2-3 times, mixing the decoctive solution and concentrating the solution to viscous liquid, adjusting an alcohol concentration of ethanol to be 60 percent to 70 percent, precipitating, filtrating and concentrating the liquor, adding ethanol and adjusting the alcohol concentration to be 75 percent to 80 percent, sequentially performing precipitation, filtration and concentration of filter liquor, and adding distilled water and refrigerating for precipitation, filtration, and concentration; and blending the distillate of Chinese mosla herb with the collected concentrated solution of indigofera tinctoria and Chinese amphibious knotweed rhizome. The Chinese drug composition can be used for preparation of traditional Chinese veterinary drug to treat blue ear pig disease, porcine circovirus infection, pseudo rabies, piglet pullorum disease of piglet, paratyphoid of piglet, pig erysipelas and edema disease of piglet. Moreover, the Chinese drug composition has the advantages of few side effect and safe and convenient use.

The invention discloses a preparation method and possible application of flagellin FliC from salmonella abortus equi to salmonella abortus equi immunity. Through cloning and connecting a flagellin FliC gene from the salmonella abortus equi to a prokaryotic expression vector pGEX-4T-2, a prokaryotic recombinant of a protein is obtained; the prokaryotic recombinant is transformed into engineering bacteria BL-21, and the protein is subjected to induced expression and purification; the protein has an amino acid sequence in a sequence table 1; the purified FliC recombinant protein has good immunogenicity after being used for the immunization of experimental animals, can overcome the defects of low antibody level and poor safety generated by inactivated vaccines and can be used for preparing subunit vaccines for preventing and treating equine paratyphoid infection and can be used as equine animal vaccine adjuvants, thereby having good development and application prospect.

The invention discloses a henhouse disinfectant formula which consists of the following raw materials in parts by weight: 5-10% of an onion original liquid, 5-10% of a sanguisorba officinalis extract liquid, 0.5-1% of hexachlorophene, 1-3% of formalin with the formaldehyde content of 40%, 0.5-1% of sodium dichloroisocyanurate, and 0.01%-0.02% of an additive named 'Chuqin'an' according to the Chinese pronunciation; a henhouse disinfectant is prepared by mixing and stirring evenly the raw materials according to the proportion. The onion original liquid and the sanguisorba officinalis extract liquid are used as the main raw materials of the disinfectant, the onion original liquid has the effect of killing staphylococcus aureus, corynebacterium diphtheriae and the like, the sanguisorba officinalis extract liquid can kill various strains of typhoid fever or paratyphoid fever pathogens and shigella dysenteriae in one minute, and the two components cooperate with a chemical disinfectant, so that the disinfectant cannot make pathogenic bacteria generate the drug resistance and has strong disinfection effect and the like.

An outer membrane vaccine for preventing the infection of typhoid bacillus and paratyphoid bacillus A and B is disclosed. Its preparing process includes such steps as culturing said bacilli in culture liquid, getting their thallus, and preparing vaccine.

The invention discloses bacillus subtilis and application thereof in a microbial agent and pork pig feed. The collection number of the bacillus subtilis in the China General Microbiological Culture Collection Center is CGMCC No.15941; the collection date is June 12, 2018; the category is Bacillus subtilis; the bacillus subtilis has a stronger function of resisting pathogenic bacteria. The invention also discloses the application of the bacillus subtilis in preparing a microbial agent for treating pork pig paratyphoid fever, wherein the pig paratyphoid fever can be perfectly relieved and prevented for the pork pig eating the microbial agent for treating pig paratyphoid fever. The invention also discloses the application of the bacillus subtilis in preparing a microbial agent for treating pig epidemic diarrhea, wherein the pig epidemic diarrhea can be perfectly relieved and prevented for the pork pig eating the microbial agent for treating pig epidemic diarrhea. The invention further discloses pork pig feed added with the bacillus subtilis, wherein the weight gain of the pork pig can be accelerated, the feed conversion ratio is reduced, and the production cost is lowered.

The invention discloses a method for rapidly and quantitatively analyzing type B paratyphoid salmonella in cucumbers on basis of prediction models. The method is based on traditional microbiology culture, growth curves of the type B paratyphoid salmonella in cucumbers at different temperatures of 10 DEG C, 15 DEG C, 20 DEG C, 25 DEG C, 30 DEG C and 35 DEG C are simulated in combination with mathematics, statistics and other subjects, first-stage and second-stage prediction models are constructed, risk prediction can be performed on initial bacterium carrying amount of commercial cucumber samples by using the models, the safety and quality of the cucumbers in a series of links of production, processing, transportation, selling, storage and the like are guaranteed, and technical support is provided for safety control of the cucumbers.

The invention relates to an application of pineal scale powder in a feed additive for pigs, belonging to an application of pineal scale powder, in particular relating to an application of pineal scale powder in the field of a feed additive for pigs. The pineal scale powder comprises the following raw materials in parts by weight: 78-82 parts of pineal scale powder, 4-6 parts of calcium hydrophosphate, 7-9 parts of zeolite powder, 0.8-1.2 parts of anilazine, 0.8-1.2 arts of bacillus subtillis, 0.5-1.5 parts of bacillus licheniformis, 0.5-1.5 parts of enzyme production probiotics and 2-4 parts of sodium humate. The invention has the beneficial effects that multiple microelement additives can be replaced by the pineal scale powder, so that the feed cost is lowered by 15%, the weight increment is improved by more than 12%, and the fattening time is greatly shortened; furthermore, the fur of the pigs is bright and ruddy, the constitution of the pigs is strong, the meat quality is obviously improved, and the lean meat percentage is improved; and meanwhile, the occurrence of diseases such as swine fever, swine plague, paratyphoid fever, influenza and the like can be reduced.

The invention discloses a process for preparing a typhoid and paratyphoid specific transfer factor oral preparation. The process mainly comprises the following steps of the preparation of immunogen, in vivo immunization of animals, and in vitro immune animal lymphocyte, the preparation of the specific transfer factors of typhoid and paratyphoid, etc. The oral preparation has the functions of transferring cell information, mediating cell immunoreaction, inhibiting specificity and killing salmonella of typhoid and paratyphoid, and also has the function of strengthening nonspecific immunity; the oral preparation has the advantages of no allergic reaction after being taken, safe use and obvious effect, and no drug resistance is generated in a patient who takes the oral preparation; and the product which adopts the oral dosing is not limited by drug taking condition, so that the product is convenient without affecting the biological activity of effective ingredients, is a safe and effective preparation and has good social value and market application prospect.

The invention relates to a traditional Chinese medicine oral liquid for treating chicken paratyphoid fever and a preparation method thereof. The traditional Chinese medicine oral liquid comprises the following ingredients, by weight, 1-5 parts of coke hawthorn, 1-5 parts of honeysuckle flower, 1-5 parts of humifuse euphorbia herb, 1-5 parts of coptis, 1-5 parts of Chinese gall, 1-10 parts of green tangerine peel, 1-5 parts of herba pteridis multifidae and 1-10 parts of licorice. The traditional Chinese medicine oral liquid has effects of antisepsis and anti-inflammation, has advantages of convenient use, low cost, fast effectiveness, little toxic and side effect and almost no residue of veterinary drug, and is an ideal traditional Chinese medicine oral liquid for treating chicken paratyphoid fever.

The invention provides a primer for detecting reverse transcription fluorescence PCR (polymerase chain reaction) of salmonella paratyphi A, which comprises 5'-CCTGTTAGAAGTGTTTACAACTT-3' and 5'-GCTAAACCACCAAATTGTGT-3'. The invention further provides a kit which contains the primer and is used for detecting the salmonella paratyphi A. The primer is a specificity primer which is designed for hsdM genes of the salmonella paratyphi A. In the reverse transcription fluorescence PCR for detecting the target genes, 44 types of salmonella paratyphi A detected by the method are positive, amplification results of other 34 types of non-salmonella typhi blood serum, other 5 types of pathogenic entero bacteria causing diarrhea and 8 types of common non-salmonella mainly causing fever are negative, accordingly, the method is high in specificity and sensitivity, and lower limit of detection of the reverse transcription fluorescence PCR is 5pg / reaction.

The present invention discloses an application of typhoid and paratyphoid salmonella in the anti-tumor aspect, which is invented to solve the problems of great side effects and poor effects of the prior art in the treatment of tumors. The present invention provides the application of the typhoid and paratyphoid salmonella in the rapid anti-tumor aspect. Wherein, the tumors are one or more of gastric cancer, lung cancer, liver cancer, tongue squamous cancer, breast cancer, colon cancer, leukemia, brain tumor and sarcoma. The invention makes use of the rapid combination of the typhoid and paratyphoid salmonella with the mononuclear cells after entering into the human body, the strong endotoxin and bacteremia are released and the temperature reaches highly around 40 DEG C to 42 DEG C, so as to affect and inhibit the growth and the life cycle of the tumor cells, furthermore, the large mononuclear cells-macrophages in the reticuloendothelial system are proliferated greatly, thus enhancing the ability of the macrophages in vivo to swallow the cancer cells, so the invention has very significant inhibiting and killing effects of a variety of tumors.

An outer membrane vaccine for preventing the infection of typhoid bacillus and paratyphoid bacillus A and B is disclosed. Its preparing process includes such steps as culturing said bacilli in culture liquid, getting their thallus, and preparing vaccine.

The invention provides a strain of cross-genus virulent bacteriophage, and the preservation number of the bacteriophage is CCTCC NO: M 2020838. According to the invention, a strain of cross-genus infected salmonella pullorum and salmonella choleraesuis bacteriophage is separated for the first time, and compared with a single lytic salmonella bacteriophage, the bactericidal range of the bacteriophage is greatly widened, and the bacteriophage has good lytic ability for a salmonella choleraesuis virulent strain C78-2, a attenuated vaccine strain C500, salmonella pullorum C79-13, S06004, RKS5078 and CDC1983-67. The bacteriophage disclosed by the invention has the characteristics of high sterilization speed and high yield. Meanwhile, the cross-genus virulent bacteriophage can be drunk with water, can eliminate animal gastrointestinal tract salmonella, is used for preventing and treating swine paratyphoid fever and pullorum disease, and is convenient to use.

The invention relates to a compound maquindox injection for veterinary use and a preparation method thereof. The injection is made from maquindox, atropine sulfate, organic solvent and water. As a special compound preparation for veterinary use, the injection has special efficacy on severe diseases caused by treponema such as swine dysentery, piglet yellow dysentery, white dysentery, calf diarrhea, paratyphoid, bacterial enteritis, and the like, and has the advantages of convenient use, short treatment course, low drug resistance, and the like.

The invention discloses a precise control method for a typhoid and paratyphoid intestinal infectious disease epidemic situation in the technical field of infectious disease control. Through the comprehensively utilization of the theories of five etiology subjects of epidemiology, environmental hygiene, microbiology, molecular biology, and lemology, the etiology differentiation and fusion two-way result conclusion of the propagation circulation or pathogen flow is obtained, investigation and analysis improve the etiologic research accuracy, the pathogen type and source are clear, and the basisis provided for pollution source determination, transmission links and cross-region diffusion; through the dividing of the spreading risk cases and carriers and the public health management, the infection sources is divided into highly-spread risk cases and carriers and general cases and carriers, the highly-spread risk cases and carriers and general cases and carriers are subjected to the publichealth management including hierarchical isolation treatment, hospital treatment, excrement detection and carrying state clearing program, therefore, the method is more scientific and targeted.

The invention belongs to the technical field of poultry breeding feed, and particularly relates to a feed formula for poultry broiler breeding, which comprises feed components and Chinese herbal medicine auxiliary material components, the feed component consists of the following raw materials in parts by weight: 38-55 parts of corn, 20-22 parts of soybean meal, 6-8 parts of peanut cakes, 3-5 parts of bran, 3-5 parts of fish meal, 9-12 parts of limestone flour, 1-3 parts of calcium hydrogen phosphate, 0.2-0.5 part of zinc sulfate, 0.1-0.5 part of methionine, 0.1-0.5 part of lysine, 0.1-0.5 part of vitamins for chickens, 0.5-2 parts of vegetable oil and 2-3 parts of an additive. According to the invention, mineral elements necessary for livestock and poultry are supplemented, so that the broiler chicken achieves relatively good nutrient balance, the weight gain of the broiler chicken is increased, and the chicken flavor is improved; the Chinese herbal medicine auxiliary material components are used for replacing antibiotics, so that chicken bacterial diseases, chicken paratyphoid fever and other chicken diseases can be prevented and treated, poultry broilers have excellent disease resistance and high survival rate, and meanwhile, the weight gain of the broilers is further improved.

Feed used for preventing swine paratyphoid fever is provided. The feed includes corn, wheat middlings, rice bran, vinasse powder, soybean meal, cotton seed meal, bone meal, imported fish meal, soybean oil, table salt, a premix and a feed additive. Through improving a raw material ratio, contents of protein and other nutrients are increased, nutrition is richer, immunity of pigs is boosted and pigs are more preferred by consumers and safer. The feed comprises the feed additive, and can achieve pest killing and sterilization so that the feed can effectively prevent the swine paratyphoid fever. The feed can improve pig quality, can effectively promote growth of pigs, and can greatly reduce the incidence rate of the swine paratyphoid fever, thus ensuring benefit of farmers.

The present invention is drawn to a live, attenuated S. Paratyphi A strain, a live, attenuated S. Paratyphi A strain comprising a stabilized plasmid expression system, and methods of using these strains.

The invention discloses a compound for treating paratyphoid fever of cat and a preparation method thereof, and aims to provide a compound for treating paratyphoid fever of cat and reduce probability of bacterial drug resistance occurrence and a preparation method thereof. The compound comprises the following components in percentage by weight: 2 to 10 percent of florfenicol, 1 to 5 percent of ofloxacin, 3 to 10 percent of taurine, 2 to 10 percent of sodium chloride, 2 to 10 percent of potassium chloride, 1 to 5 percent of vitamin K3, and the balance of glucosum anhydricum. In the compound, the florfenicol and the ofloxacin serving as antibacterial components can effectively kill salmonella, and is effective to escherichia coli and other bacteria, and combination of the florfenicol and the ofloxacin can reduce the probability of bacteria drug resistance, so that the effect of sterilization and bacteriostasis is reinforced; the taurine has the functions of resisting inflammation, protecting liver and abating fever, and an effect of symptomatic treatment to clinical symptoms caused by paratyphoid fever of cat; the sodium chloride and the potassium chloride can supply electrolyte; the vitamin K3 has an effect of stopping bleeding; and the drugs are combined to treat the paratyphoid fever of cat from various aspects, so that therapeutic errors are reduced.

The invention relates to the technical field of traditional Chinese medicines, and particularly relates to a traditional Chinese medicine composition capable of preventing paratyphoid fever of lambs, and a preparation method of the composition. The traditional Chinese medicine composition is applicable to treatment of diseases of bloody flux, black scour and the like of lambs. The prescription is prepared from the following traditional Chinese medicines: lonicerae flos, radix pulsatillae, cortex phellodendri, pericarpium papaveris, and has the functions of clearing away heat and detoxifying, arresting and inducing astringency, invigorating the spleen for eliminating dampness and harmonizing the stomach and checking diarrhea. The purpose of treating the paratyphoid fever of the lambs can be achieved. The invention provides a preparation method and applications of the traditional Chinese medicine composition. The traditional Chinese medicine composition has the functions of clearing away heat and detoxifying, arresting and inducing astringency, invigorating the spleen for eliminating dampness and harmonizing the stomach and checking diarrhea. By adopting the traditional Chinese medicine and veterinarian therapy and using pure traditional Chinese medicines, the following traditional Chinese medicines are orally taken by the lambs, and the lambs are healed fast without side effects, thus being beneficial to the growth of lambs. The traditional Chinese medicine liquor is simple to blend, and convenient to use, the cost of raw materials is low, no toxic reaction occurs after use, the healing rate is high, and no residues exist, so that the traditional Chinese medicine composition is a desirable drug for treating the paratyphoid fever of the lambs.

A medicine for treating chicken paratyphoid fever is disclosed. The medicine is prepared by adding water into 40 g of teucrium viscidum Bl., 30g of purslane herb, 30 g of creeping euphorbia, 30 g of yerbadetajo herb, 45 g of dandelion, 20 g of plantain herb, 30 g of virgate wormwood herb, 30 g of Chinese bellflower and 20 g of herba houttuyniae, and decocting the materials for 30 min to obtain a liquid, and the liquid is adopted as the medicine. During use, when chickens in a house are affected with the chicken paratyphoid fever, the medicine is prepared twice a day according to the above amounts and is drunk by one hundred chickens themselves or mixed with feed and fed to the chickens, seriously ill chickens are drenched with 10 mL of the medicine and then the chickens can be cured after 3 days. Through clinical application, chickens of more than 100 houses are cured with the medicine.

The invention provides a feed formula of ewes in the lactation period. Every 1000 parts of the feed comprise, by weight, 390-440 parts of corn, 160-190 parts of silage corn, 200-240 parts of soybean meal, 40-60 parts of palm kernel meal, 90-110 parts of dry clover, 20-40 parts of grass meal, 20-40 parts of beet pulp, 1-3 parts of Herba Leonuri, 1-3 parts of bone meal, 1-3 parts of zeolite powder, 1-2 parts of montmorillonite, 1-2 parts of calcium hydrogen phosphate, 1-3 parts of sodium bicarbonate, 1-2 parts of magnesium oxide, 1-2 parts of zinc sulfate and 2-3 parts of salt. The feed formula has the advantages of reasonable components, complete nutrition, improvement of the palatability of the feed, stimulation of the appetite of the ewes, realization of effective increase of the milk yield due to the addition of proper amounts of medicinal materials, prevention of the paratyphoid fever disease, colibacillosis and other common diseases of lambs in a n ewe intake and lactation manner, realization of full nutrition of the ewes and the lambs, and realization of the healthy growth of the lambs.

The invention discloses a hepatitis-B-paratyphoid-fever-resistant O-SP monoclonal antibody, a hybridoma cell strain and an application. The hybridoma cell strain is named WV-SPB-11, and the collection number is CCTCC NO:C2015228. The monoclonal antibody is secreted through the hybridoma cell strain in the claim 1. The monoclonal antibody is high in purity and antibody titer, and the following experimental requirement is met; the polysaccharide content of hepatitis-A paratyphoid fever O-SPO-SP is measured with the indirect competitive ELISA method built by the monoclonal antibody, the specificity and the sensitivity are high, and particularly, the monoclonal antibody and hepatitis-A paratyphoid fever O-SP polysaccharide, OSP polysaccharide combination and the like are not subjected to a cross reaction.

The invention discloses a traditional Chinese drug composition for pig and a preparation method and application thereof. The composition comprises drug substances of 6-18 parts of indigofera tinctoria by weight, 4-12 parts of Chinese amphibious knotweed rhizome by weight and 5-15 parts of Chinese mosla herb by weight. The preparation method of the composition comprises: distilling Chinese mosla herb, and collecting the distillate; adding indigofera tinctoria and Chinese amphibious knotweed rhizome in the residue and residual liquid after distillation and decocting for 2-3 times, mixing the decoctive solution and concentrating the solution to viscous liquid, adjusting an alcohol concentration of ethanol to be 60 percent to 70 percent, precipitating, filtrating and concentrating the liquor,adding ethanol and adjusting the alcohol concentration to be 75 percent to 80 percent, sequentially performing precipitation, filtration and concentration of filter liquor, and adding distilled waterand refrigerating for precipitation, filtration, and concentration; and blending the distillate of Chinese mosla herb with the collected concentrated solution of indigofera tinctoria and Chinese amphibious knotweed rhizome. The Chinese drug composition can be used for preparation of traditional Chinese veterinary drug to treat blue ear pig disease, porcine circovirus infection, pseudo rabies, piglet pullorum disease of piglet, paratyphoid of piglet, pig erysipelas and edema disease of piglet. Moreover, the Chinese drug composition has the advantages of few side effect and safe and convenient use.

The invention relates to a kit for separating and identifying salmonella, and a preparation and an application thereof, which are mainly used for solving the technical difficult problems of high omission ratio, complicated identification steps, higher cost and non-intuitive screening result of an existing salmonella separation method. The kit disclosed by the invention comprises general-purpose type non-selective enrichment broth, general-purpose type non-selective enrichment broth which is concentrated by 10 times, selenite sulfonamide green-sulfanilamide enrichment broth, a xylose lysine deoxycholate agar plate, a salmonella enzyme chromogenic agar plate, a salmonella biochemical identification tube, a hydrogen sulfide filter paper strip and an indole filter paper strip, and is characterized by further comprising improved Robert enrichment broth which comprises the following components: 13.58g of magnesium chloride, 7.2g of sodium chloride, 4.5g of papain digestion soybean peptone, 1.26g of KH2PO4, 0.18g of K2HPO4, 36mg of malachite green and 1000ml of distilled water. The kit disclosed by the invention is mainly designed for synchronous separation and identification of salmonella (including typhoid, paratyphoid and non-typhoid and paratyphoid salmonella) in enteric pathogenic bacteria.