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Bacillus subtilis and application thereof in microbial agent and pork pig feed

A Bacillus subtilis and bacterial agent technology, applied in the field of microorganisms, can solve the problems of limited anti-pathogenic bacteria functions of natural probiotics, and achieve the effects of accelerating weight gain, strengthening the ability to resist pathogenic bacteria, and reducing the ratio of feed to meat

Active Publication Date: 2018-11-13
GENE FOCI BEIJING BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the anti-pathogenic bacteria function of natural probiotics is very limited, so it is necessary to adopt methods such as induced mutations to enhance variation, so it is necessary to provide a bacillus that is applied to pig digestive system diseases

Method used

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  • Bacillus subtilis and application thereof in microbial agent and pork pig feed
  • Bacillus subtilis and application thereof in microbial agent and pork pig feed

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0069] In a pig farm with a stock of 30,000 heads in Jiangxi Province, large-scale infectious diarrhea occurred in early spring, which occurred in sows, piglets and fattening pigs, but the incidence rate was highest in piglets before weaning. Typical symptoms include: intermittent or persistent diarrhea, the feces are grayish-yellow mucus, and the area around the anus, tail, and hindquarters are contaminated with loose feces, which dry and form feces or dry fecal balls attached to the hindquarters or tail. Sick pigs have poor appetite, growth stagnation, and eventually die or form stiff pigs. After the outbreak, the farm failed to effectively control the spread of the epidemic through a variety of antibiotics, other chemical drugs, and traditional Chinese medicine treatment programs, resulting in the death of more than 500 pigs within 20 days, with direct and indirect economic losses exceeding 1 million yuan. Recommended by a friend, the farm tried the GF118 Bacillus subtilis ...

Embodiment 2

[0073] In a pig farm in Anhui, the phenomenon of water spit in meat pigs occurs frequently, especially in piglets. After analysis, it is suggested that all antibiotics should be stopped and GF118 Bacillus subtilis should be used instead. Feed should be supplemented with 20 billion live bacteria / g of GF118 Bacillus subtilis at 200-300g per ton for continuous use. From the 3rd day, the phenomenon of spit water was alleviated, and after 5 days of continuous use, the symptom of spit water basically disappeared.

[0074] Among them, the pig feed contains the following components by weight ratio: acorn powder 700-750, wheat bran 230-250, corn flour 120-130, shrimp shell powder 12-14, ginger 4-5, Wujiapi 2- 3. Rehmannia glutinosa 10-12, astragalus 2-3, epimedium 1-2, malt 3-4, Tianjihuang 2-3, white lentils 10-12, glutinous rice 2-3, thorn pear root 1-2 And an umbrella 2-3.

Embodiment 3

[0075] Example 3: Optimizing effect of GF118 Bacillus subtilis instead of antibiotics on breeding indicators such as pig weight gain and reduction of feed-to-meat ratio

[0076] A pig farm in Beijing used GF118 Bacillus subtilis instead of antibiotics. From 21 days after weaning, 200-300g per ton of GF118 Bacillus subtilis was added to the feed, and continued to use until the pigs were slaughtered. Bacillus subtilis, experimental components: experimental group 1, experimental group 2 and experimental group 3, experimental group 1 was added 20 billion live bacteria / g GF118 Bacillus subtilis at 200 g per ton of feed; experimental group 2 was added per ton of feed 20 billion live bacteria / g of 250g of GF118 Bacillus subtilis; experiment 3 groups added 300g of 20 billion live bacteria / g of GF118 Bacillus subtilis per ton of feed; the control group, experiment 1 group, experiment 2 and experiment The three groups were compared with the two key indicators of weight gain and feed-to-...

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Abstract

The invention discloses bacillus subtilis and application thereof in a microbial agent and pork pig feed. The collection number of the bacillus subtilis in the China General Microbiological Culture Collection Center is CGMCC No.15941; the collection date is June 12, 2018; the category is Bacillus subtilis; the bacillus subtilis has a stronger function of resisting pathogenic bacteria. The invention also discloses the application of the bacillus subtilis in preparing a microbial agent for treating pork pig paratyphoid fever, wherein the pig paratyphoid fever can be perfectly relieved and prevented for the pork pig eating the microbial agent for treating pig paratyphoid fever. The invention also discloses the application of the bacillus subtilis in preparing a microbial agent for treating pig epidemic diarrhea, wherein the pig epidemic diarrhea can be perfectly relieved and prevented for the pork pig eating the microbial agent for treating pig epidemic diarrhea. The invention further discloses pork pig feed added with the bacillus subtilis, wherein the weight gain of the pork pig can be accelerated, the feed conversion ratio is reduced, and the production cost is lowered.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a bacillus subtilis and its application in bacterial agents and pig feed. Background technique [0002] Pork and related by-products are an important part of our country's dietary structure, so the pig industry is an important agricultural pillar industry related to the national economy and people's livelihood. However, various bacterial and viral infectious diseases such as blue ear disease, viral diarrhea, cholera, Tuberculosis, clostridial enteritis, etc. have become a huge hazard to the modern aquaculture industry. Antibiotics are widely used as disease prevention and growth-promoting agents, which greatly reduce the morbidity, mortality and production costs of animal breeding. The intensive farming method has greatly improved the production efficiency of the farming industry, but the overcrowded and unsanitary environment also increases the risk of animal infectious ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A61K35/742A61P31/04A61P1/12A23K10/18C12R1/125
CPCA61K35/742A23K10/18A61P1/12A61P31/04C12N1/205C12R2001/125
Inventor 杨波刘川浩郭春雨杨建赵阳
Owner GENE FOCI BEIJING BIOTECH CO LTD
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