116 results about "Salmonella Pullorum" patented technology

The invention provides an Enterococcus faecium ANSE228 of which the collection number is CGMCC No.4082. The invention also provides application of the Enterococcus faecium ANSE228 to inhibition of salmonella pullorum and / or Escherichia coli and / or Staphylococcus aureus. The Enterococcus faecium ANSE228 is obtained by processes of repeated separation, purification, rejuvenation and the like, and has high biological activity, obvious probiotic property, high adversity resistance and the like. The invention also provides a microecological agent which contains the Enterococcus faecium ANSE228. When the microecological agent is added into drinking water and / or feeds for breeding animals, the Enterococcus faecium ANSE228 can be quickly activated and reproduced and a dominant beneficial flora can be formed after the Enterococcus faecium ANSE228 is fed into intestinal canals of the animals, and the Enterococcus faecium ANSE228 has the effects of reducing a harmful flora in the intestinal canals, adjusting microecological balance of the intestinal canals, substituting for medicaments such as antibiotic and the like, and improving weight increment of the animals and the utilization rate of the feeds.

The invention provides an Enterococcus faecium ANSE228 of which the collection number is CGMCC No.4082. The invention also provides application of the Enterococcus faecium ANSE228 to inhibition of salmonella pullorum and / or Escherichia coli and / or Staphylococcus aureus. The Enterococcus faecium ANSE228 is obtained by processes of repeated separation, purification, rejuvenation and the like, and has high biological activity, obvious probiotic property, high adversity resistance and the like. The invention also provides a microecological agent which contains the Enterococcus faecium ANSE228. When the microecological agent is added into drinking water and / or feeds for breeding animals, the Enterococcus faecium ANSE228 can be quickly activated and reproduced and a dominant beneficial flora can be formed after the Enterococcus faecium ANSE228 is fed into intestinal canals of the animals, and the Enterococcus faecium ANSE228 has the effects of reducing a harmful flora in the intestinal canals, adjusting microecological balance of the intestinal canals, substituting for medicaments such as antibiotic and the like, and improving weight increment of the animals and the utilization rate of the feeds.

Disclosed herein is a novel bacteriophage which has specific bactericidal activity against one or more Salmonella bacteria selected from the group consisting of Salmonella Enteritidis, Salmonella Typhimurium, Salmonella Gallinarum, and Salmonella Pullorum without affecting beneficial bacteria, in addition to showing excellent tolerance to acid, heat and desiccation. The novel bacteriophage can be widely used as an active ingredient for therapeutic agents, animal feeds or drinking water, cleaners and sanitizers for preventing and treating the infectious diseases caused by Salmonella Enteritidis, Salmonella Typhimurium, Salmonella Gallinarum or Salmonella Pullorum including salmonellosis, Salmonella food poisoning, Fowl Typhoid, and Pullorum disease or for controlling the salmonella bacteria.

The present invention relates to a novel bacteriophage, more particularly, a bacteriophage that has a specific bactericidal activity against one or more Salmonella bacteria selected from the group consisting of Salmonella Enteritidis, Salmonella Typhimurium, Salmonella Gallinarum, and Salmonella Pullorum. Further, the present invention relates to a composition for the prevention or treatment of infectious diseases including salmonellosis and Salmonella food poisoning caused by Salmonella Enteritidis or Salmonella Typhimurium, Fowl Typhoid caused by Salmonella Gallinarum, and Pullorum disease caused by Salmonella Pullorum, comprising the bacteriophage as an active ingredient. Furthermore, the present invention relates to a feed additive, drinking water, a cleaner and a sanitizer, comprising the bacteriophage as an active ingredient.

The invention relates to a Salmonella bacteriophage, particularly a wide-host-spectrum strongly-lytic Salmonella pullorum bacteriophage. The invention also relates to Salmonella pullorum for controlling the bacteriophage in a culture environment. The Salmonella pullorum bacteriophage is named PSPu-4-116, and collected in China Center for Type Culture Collection, and the collection number is CCTCC M2011042.

The invention discloses a multiple PCR identification kit of salmonella and five serotypes of salmonella. The invention provides a primer group for detecting salmonella and five serotypes of salmonella. The primer group comprises a primer pair 1, a primer pair 2, a primer pair 3, a primer pair 4, a primer pair 5 and a primer pair 6. Aiming at specific fragments of salmonella and its five important serotypes (such as salmonella typhimurium, enteritidis, agona, choleraesuis and pullorum), the six pairs of the primers for specific amplification are designed, the amplified fragments comprise 425bp of salmonella, 198bp of salmonella typhimurium, 302bp of salmonella enteritidis, 145bp of salmonella agona, 368bp of salmonella choleraesuis and 249bp of salmonella pullorum, and a sample gene group can be accordingly determined by multiple PCR and electrophoresis detection. Through six pairs of the specific primers and one-step multiple PCR, the salmonella and its five important serotypes can be identified. The multiple PCR identification kit has the characteristics of fast identification rate, low cost, easy operation and easy result determination.

The invention relates to a multiple PCR (polymerase chain reaction) method for identifying salmonella enteritidis, salmonella typhimurium, salmonella pullorum and salmonella gallinarum. The multi-PCR method comprises the following steps of: with the extracted DNA (deoxyribonucleic acid) of a bacterium to be detected as a template, carrying out mPCR (multiple polymerase chain reaction) amplification on five pairs of primers as shown in SEQ ID NO.1-10; and carrying out agarose gel electrophoresis on mPCR amplification products, and then determining according to electrophoresis results. According to the multiple PCR method, the synthesized primers are used for carrying out PCR amplification, a diseased material can be directly ground and amplified by adopting the established multiple PCR, different salmonella serotypes can be detected in one system, serum glass plate agglutination is completed in only about six hours compared with three days for the traditional serum glass plate agglutination after separation pure culture in clinical detection, the diagnosis time is greatly shortened, and a basis is provided for clinical diagnosis.

An ELISA kit for detecting a Salmonella pullorum antibody is established by screening Salmonella pullorum dominant antigen GroEL through an immunoprecipitation technology, expressing GroEL recombinant protein through utilizing a prokaryotic expression vector, and utilizing an antigen protein. The kit can reduce the response of chicken in the detection process of the Salmonella pullorum antibody, and can improve the detection specificity and the sensitivity.

The invention discloses a pullorum staining agglutination antigen as well as a preparation method and an application thereof, and belongs to the technical field of veterinarian diagnosis. Salmonella pullorum SP7220, SP7701, SP8441 and SP9905 are subjected to recovery and passage respectively to form a seed bacterium liquid, then the seed bacterium liquid is inoculated with a solid culture medium for proliferation, after inactivation with formalin, the concentration of the bacterium liquid is adjusted with turbidimetry, finally, the bacterium is fully and evenly mixed and packaged after a crystal violet solution is added for staining, and the pullorum staining agglutination antigen is prepared. The technological method is simple, reasonable, scientific, stable in production and low in cost, the selected production stains of the pullorum staining agglutination antigen are good in antigenicity and low in mutation rate, the prepared pullorum staining agglutination antigen product has the advantages of high sensitivity, high specificity, quickness in diagnosis and clearness in agglutination image, the staining agglutination antigen is relatively ideal for pullorum detection.

The present invention relates to a novel bacteriophage, more particularly, a bacteriophage that has a specific bactericidal activity against Salmonella enteritidis, Salmonella typhimurium, Salmonella gallinarum and Salmonella pullorum, a composition for the prevention or treatment of infectious diseases including salmonellosis and Salmonella food poisoning caused by Salmonella enteritidis or Salmonella typhimurium, Fowl typhoid caused by Salmonella gallinarum, and Pullorum disease caused by Salmonella pullorum, which comprises the bacteriophage as an active ingredient, and an animal feed, drinking water, cleaner, and sanitizer which comprise the bacteriophage as an active ingredient.

The invention discloses an attenuated Salmonella pullorum and application thereof. By using gene knockout technology, the in vivo colonization gene and the main virulence gene of Salmonella pullorum are deleted, thus obtaining an dual-gene-deleted attenuated Salmonella pullorum SM091-DED strain; and the microbiological preservation number of the strain is CGMCC NO.4604. The virulence of the attenuated Salmonella pullorum disclosed by the invention is obviously reduced, and the in vivo colonization time is short after the attenuated Salmonella pullorum is inoculated into a host; the herd infection test shows that the attenuated Salmonella pullorum has no horizontal transmission capability; the attenuated Salmonella pullorum provides full cross protection for homotype and allotype high-virulence Salmonella pullorum; and after SPF (Specific Pathogen Free) chicks are immunized, the infection of the high-virulence strain can be effectively eliminated, and the herd infection can not be caused. Thus, the invention provides a safe, fine-immunogenicity and low-virulence Salmonellosis avium attenuated live vaccine for preventing Salmonellosis avium.

The invention provides a salmonella broad-spectrum virulent bacteriophage. The preservation number of the bacteriophage is CCTCC NO: M 2020839. The invention also provides a preparation process and application of the salmonella broad-spectrum virulent bacteriophage, and the preparation process comprises fermentation and extraction; in the step of fermentation, a salmonella gallinarum attenuated vaccine strain is used as host bacteria for bacteriophage fermentation. The bacteriophage XPARCPS02 disclosed by the invention can be used for cracking salmonella of various serotypes and also has different degrees of cracking effects on drug-resistant bacteria; the salmonella typhimurium lysate lysate has a cracking capability on salmonella typhimurium, salmonella typhimurium drug-resistant bacteria, salmonella gallinarum, salmonella enteritidis, salmonella enteritidis drug-resistant bacteria, salmonella typhimurium, salmonella pullorum, salmonella paratyphi A, salmonella paratyphi B and salmonella dubicularis.

Disclosed herein is a novel bacteriophage which has specific bactericidal activity against one or more Salmonella bacteria selected from the group consisting of Salmonella enteritidis, Salmonella typhimurium, Salmonella gallinarum, and Salmonella pullorum without affecting beneficial bacteria, in addition to showing excellent tolerance to acid, heat and desiccation. The novel bacteriophage of the present invention can be widely used as an active ingredient for therapeutic agents, animal feeds or drinking water, cleaners and sanitizers for preventing and treating the infectious diseases caused by Salmonella enteritidis, Salmonella typhimurium, Salmonella gallinarum or Salmonella pullorum including salmonellosis, Salmonella food poisoning, Fowl Typhoid, and Pullorum disease or for controlling the Salmonella bacteria. The present invention also provides important insights into prevention and control strategies against Salmonella infection and suggests that the use of bacteriophage can be a novel, safe, and effectively plausible alternative to antibiotics for the prevention of Salmonella infection in poultry.

Disclosed herein are is a novel bacteriophage which has specific bactericidal activity against one or more Salmonella bacteria selected from the group consisting of Salmonella Enteritidis, Salmonella Typhimurium, Salmonella Gallinarum, and Salmonella Pullorum without affecting beneficial bacteria. Disclosed are also compositions, animal feeds or drinking water, cleaners and sanitizers for preventing and treating the infectious diseases caused by Salmonella Enteritidis, Salmonella Typhimurium, Salmonella Gallinarum or Salmonella Pullorum including salmonellosis, Salmonella food poisoning, Fowl Typhoid, and Pullorum disease or for controlling the salmonella bacteria.

The invention provides an environment biological disinfectant with phages as main components and application thereof. The biological disinfectant is composed of Escherichia coli phage CCTCC (China Center for Type Culture Collection) M209208, Bacterium enteritidis phage CCTCC M2010226, Salmonella pullorum phage CCTCC M2011042, Shigella flexneri phage CCTCC M209029, cinnamon extract, Chinese pulsatilla extract and auxiliary agents, wherein the purified phages are prepared into liquid with the concentration of more than or equal to 10<4> pfu (plaque forming unit) / mL, and are used for sterilizing and cleansing environment places and devices. The biological disinfectant has the characteristics of strong sterilization capability, rapid sterilization, no toxicity or harm, no irritation, no corrosion, no colorizing performance and the like, is especially suitable for disinfection and cleansing of breeding farms, slaughter houses, livestock product processing factories, food warehouses and other environments and devices, and has better popularization and use values.

Disclosed herein are is a novel bacteriophage which has specific bactericidal activity against one or more Salmonella bacteria selected from the group consisting of Salmonella Enteritidis, Salmonella Typhimurium, Salmonella Gallinarum, and Salmonella Pullorum without affecting beneficial bacteria. Disclosed are also compositions, animal feeds or drinking water, cleaners and sanitizers for preventing and treating the infectious diseases caused by Salmonella Enteritidis, Salmonella Typhimurium, Salmonella Gallinarum or Salmonella Pullorum including salmonellosis, Salmonella food poisoning, Fowl Typhoid, and Pullorum disease or for controlling the salmonella bacteria.

The invention provides a hybridoma cell strain capable of secreting IpaJ monoclonal antibody of salmonella pullorum, and a monoclonal antibody and application thereof. The hybridoma cell strain capable of secreting IpaJ monoclonal antibody of salmonella pullorum is preserved in CCTCC. The monoclonal antibody secreted by the hybridoma cell strain has the advantages of high valence, good specificityand high in affinity to natural antigens. A competitive ELISA detection method for the IpaJ monoclonal antibody of salmonella pullorum has relatively good sensitivity and specificity and is used forevaluating the immune state of a chicken body and researching related infectious diseases.

The invention discloses a salmonella pullorum SP9905. The strain is preserved at China Center for Type Culture Collection with an address of Wuhan University on October 15, 2014 and the preservation number is CCTCC NO.M 2014478. The invention also discloses an application of the salmonella pullorum in preparation of a live vaccine. The vaccine provided by using salmonella pullorum SP9905 has the advantages of high viable count and low freeze-drying death rate; the product is safe and reliable; a strong protecting force can be generated in the fourteenth day due to an immune protection effect, the immune protective rate is 100% and a good protective capability (80-100%) can be obtained for the other serotype salmonella (salmonella enteritidis and salmonella gallinarum).

The invention discloses rare earth metal complexes using orbifloxacin as a ligand, a method for synthesizing the same and application thereof. In the tests of the inhibiting effect of the complexes on bacteria such as salmonella pullorum, swine streptococcosis and bovine streptococcus uberis, the results show that the complexes have quite high bacteriostatic activity, wherein all of Er(III), Sm(III) and Eu(III) complexes of the orbifloxacin have certain bacteriostatic activity on the swine streptococcosis which cannot be inhibited by orbifloxacin and doxycycline. Mouse acute toxicity tests show that the complexes have low in-vivo toxicity to mice and are highly tolerable to mice. The toxicity of various complexes meets the acute oral toxicity ungraded standards of European Community and the PRC food security assessment grade-two (actually non-toxic) standard, wherein the neodymium complex of the orbifloxacin has the lowest toxicity.

The present invention relates to a novel bacteriophage, more particularly, a bacteriophage that has a specific bactericidal activity against Salmonella typhimurium, Salmonella gallinarum, or Salmonella pullorum, a composition for the prevention or treatment of infectious diseases including salmonellosis and Salmonella food poisoning caused by Salmonella typhimurium, Fowl typhoid caused by Salmonella gallinarum, and Pullorum disease caused by Salmonella pullorum, which comprises the bacteriophage as an active ingredient, and an animal feed, drinking water, cleaner, and sanitizer which comprise the bacteriophage as an active ingredient. The present invention also provides important insights into prevention and control strategies against Salmonella infection and suggests that the use of bacteriophage can be a novel, safe, and effectively plausible alternative to antibiotics for the prevention of Salmonella infection in poultry.

The invention provides a traditional Chinese medicine preparation for controlling pullorosis salmonellosis. The traditional Chinese medicine preparation is characterized by being prepared from the following extracts in parts by weight: 3 to 5 parts of polygonum cuspidatum extract, 1 to 2 parts of fructus chebulae extract, and 1 to 2 parts of herba violae extract. The prepared traditional Chinese medicine compound particles are uniform in size, consistent in color and luster, and free of moisture adsorption, caking, deliquescence and other phenomena, meet the requirements of Chinese Pharmacopoeia, and are controllable in quality. The pharmacodynamics study shows that the traditional Chinese medicine preparation can be used for preventing and treating pullorosis salmonellosis.

The invention belongs to the field of biotechnology detection, and particularly relates to a PCR detection kit for rapidly identifying Salmonella Pullorum / Salmonella Gullinarum, wherein the PCR detection kit comprises a SPUL_2693 gene detection primer pair, and the SPUL_2693 gene detection primer pair comprises a forward primer with a nucleotide sequence represented by SEQ ID NO.1 and a reverse primer with a nucleotide sequence represented by SEQ ID NO.2. According to the present invention, the kit can perform rapid and high-throughput identification on Salmonella Pullorum / Salmonella Gullinarum, can be used as the auxiliary method for the traditional serological typing of Salmonella, and provides the new simple, rapid and highly-sensitive method for the monitoring and laboratory diagnosisof Salmonella Pullorum / Salmonella Gullinarum.

The present invention relates to a novel bacteriophage, more particularly, a bacteriophage that has a specific bactericidal activity against Salmonella enteritidis, Salmonella typhimurium, Salmonella gallinarum and Salmonella pullorum, a composition for the prevention or treatment of infectious diseases including salmonellosis and Salmonella food poisoning caused by Salmonella enteritidis or Salmonella typhimurium, Fowl typhoid caused by Salmonella gallinarum, and Pullorum disease caused by Salmonella pullorum, which comprises the bacteriophage as an active ingredient, and an animal feed, drinking water, cleaner, and sanitizer which comprise the bacteriophage as an active ingredient.

The present invention relates to a Salmonella bacteriophage, in particular to a Siphoviridae bacteriophage with a wide host spectrum and a strong lytic activity against Salmonella pullorum. The above-mentioned Salmonella pullorum bacteriophage is named SP4, and is deposited at the China General Microbiological Culture Collection Center, the deposit date is Jul. 27, 2017 and the deposit number is CGMCC No, 14332, The bacteriophage has a strong lysis effect on Salmonella, and the bacteriophage can also reduce the mortality rate of chicks infected with Salmonella pullorum, The preparation can be used alone or as a cocktail, and provides a safe, non-toxic and side-effect-free and residual effect-free bacteriophage product for the treatment of the infections caused by Salmonella of chicken origin, Salmonella of duck origin, Salmonella of mink origin, Salmonella of food origin and Salmonella of pig origin.

The invention relates to a pullorum disease agglutination antigen and a preparation method thereof and mainly relates to a bacterial strain which is salmonella pullorum standard strain C79-1 and a screened wild isolated strain S44. The wild isolated strain S44 of salmonella pullorum is classified and named as follows: salmonella enterica intestinal sub-salmonella pullorum, of which the Latin name is Salmonella enterica subsp. enterica pullorum, which is screened from the salmonella pullorum separated from chicken and which is collected at China General Microbiological Culture Collection Center with the collection No.: CGMCC No. 14258. The method provided by the invention is scientific and reasonable, stable in production and low in cost; the selected pullorum disease agglutination antigen production strain is high in antigenicity and low in aberration rate; and the prepared pullorum disease agglutination antigen product has the advantages of high sensibility, high specificity, quick diagnosis, easiness in observing agglutination effect, and the like.

The invention discloses a avian leukemia double plate agglutination antigen of chicken pullorum disease-J subgroup and a preparation method thereof. The method includes constructing a plasmid vector displaying avian leukemia specific antigen GP85 of subgroup J on the surface, and transferring it into the strain for preparing chicken pullorum antigen to construct Salmonella pullorum which can display GP85 on the surface. The Salmonella pullorum can simultaneously express the surface antigen of Salmonella pullorum and the specific antigen of avian leukosis virus subgroup J through IPTG, and canbe used for preparing the double plate agglutination antigen. The double plate agglutination antigen can be used to detect two kinds of epidemic antibodies at the same time by one reaction. The operation is simple, the specificity is strong, the detection time and the detection cost are obviously saved, and the double antigen can be used for rapid detection of fence edge.

The present invention relates to a novel bacteriophage, more particularly, a bacteriophage that has a specific bactericidal activity against Salmonella typhimurium, Salmonella gallinarum, or Salmonella pullorum, a composition for the prevention or treatment of infectious diseases including salmonellosis and Salmonella food poisoning caused by Salmonella typhimurium, Fowl typhoid caused by Salmonella gallinarum, and Pullorum disease caused by Salmonella pullorum, which comprises the bacteriophage as an active ingredient, and an animal feed, drinking water, cleaner, and sanitizer which comprise the bacteriophage as an active ingredient.

The invention discloses a small rhzomorph MccY and a preparation method and application thereof. The amino acid sequence of the small rhzomorph MccY is GGRGHIAEYFSGPITQVSFYG. Compared with MccJ25 which only has bactericidal activity on a small part of serotype salmonella such as enteritis and the like, the small rhzomorph MccY has a bacteriostatic / bactericidal effect on salmonella pullorum, salmonella typhimurium, salmonella kafenkii, salmonella infantis, salmonella London and shigella sonnei, and can kill other salmonella serotypes which cannot be killed by MccJ25; the small rhzomorph MccY has a remarkable bactericidal effect on salmonella typhimurium and salmonella pullorum which are common in livestock and poultry production, also has a bacteriostatic / bactericidal effect on shigella sonnei, overcomes the defect of narrow spectrum of small rhzomorph, has breakthrough significance, and has the potential of being used as an antibiotic substitute.