13 results about "Salmonella Pullorum" patented technology

This invention belongs to the field of molecular biology detection technology, specifically relating to a primer and probe combination for detecting avian Salmonella based on the RAA-LFD method and its application, particularly to a primer and probe combination for detecting three avian serotypes of Salmonella: Salmonella pullorum, Salmonella enteritidis, and Salmonella typhimurium, based on the RAA-LFD method and its application. This invention designs different types of probes and primers targeting the genes of serotypes SP, SE, and STM of Salmonella. Using the primer and probe combination of this invention, rapid, sensitive, and specific detection of Salmonella serotypes and the three avian Salmonella serotypes SP, SE, and STM can be achieved, greatly shortening the detection cycle of avian salmonellosis and improving the detection efficiency and accuracy of avian salmonellosis.

The application belongs to the field of biotechnology detection, and particularly relates to a multiplex PCR detection kit for identifying and distinguishing chicken pullorum salmonella and chicken typhoid salmonella. The kit comprises stn gene detection primers, I137_14445 gene detection primers and ybgL gene detection primers. The kit can quickly, high-throughput and accurately identify and distinguish chicken pullorum salmonella and chicken typhoid salmonella, can replace traditional salmonella serological typing and biochemical identification and other complex procedures, and provides a simple, rapid, reproducible new method for monitoring and laboratory diagnosis of chicken pullorum salmonella and chicken typhoid salmonella.

The application belongs to the field of feed additives, and particularly discloses application of sodium butyrate in preparation of a feed additive against chicken white dysentery Salmonella, and sodium butyrate is added in chicken basic feed as the feed additive against chicken white dysentery Salmonella; the addition of sodium butyrate in chicken basic feed can significantly reduce the bacterial load of chicken white dysentery Salmonella infection in chicken liver and spleen, significantly increase the ratio of villus length to crypt depth of the cecum of chicken infected with chicken white dysentery Salmonella, and improve the intestinal absorption capacity; the content of CD3+ T cells, TCR+ T cells, macrophages and B cells in the liver of chicken infected with chicken white dysentery Salmonella can be significantly reduced; and then the body weight of chicken infected with chicken white dysentery Salmonella is significantly increased, and the survival rate of chicken infected with chicken white dysentery Salmonella is increased. In summary, the addition of sodium butyrate in chicken basic feed can enhance the resistance to chicken white dysentery Salmonella infection.

The invention belongs to the technical field of biological detection, and discloses a bacteriophage PQ38, application thereof and a salmonella inhibiting medicine, the bacteriophage PQ38 has the preservation number of GDMCC NO: 67169-B1, is preserved in Guangdong Microbial Culture Collection Center, the preservation time is October 27, 2025, and the bacteriophage PQ38 is named as Siphoviridae jerseyvirus in taxonomy; the bacteriophage has a good salmonella splitting capability in salmonella, and tests show that the bacteriophage has an excellent capability of splitting salmonella pullorum.

The invention discloses a nano-enzyme for preventing salmonella pullorum infection, the nano-enzyme comprises kaempferol and cerium ions, the kaempferol and the cerium ions form a kaempferol-cerium composite nano-enzyme CeKae NPs through coordination, and the nano-enzyme has superoxide dismutase (SOD)-like activity and catalase (CAT)-like activity. The invention further discloses a preparation method and application of the nano-enzyme for preventing salmonella pullorum infection. The CeKae NPs nano-enzyme provided by the invention comprises kaempferol and cerium ions, has efficient SOD and CAT nano-enzyme activity, and can realize antioxidant self-cascade nano-enzyme reaction, effectively remove active oxygen and improve the pathological environment.

The present application relates to the technical field of microorganism, and especially relates to a kind of Enterococcus durans C24, probiotic agent and application thereof.The present application provides Enterococcus durans C24, and its preservation number is CGMCC No.33995.The strain has broad-spectrum antibacterial activity, can inhibit Salmonella enteritidis, Salmonella pullorum and Salmonella typhimurium, has no hemolytic activity, and has good stress resistance, can survive in gastrointestinal environment, and also has high biological safety, and is expected to be used for clinical prevention and control of salmonellosis.

The invention discloses lactobacillus johnsonii for preventing and treating pullorum disease salmonellosis and application of the lactobacillus johnsonii, and belongs to the field of microorganisms. According to the invention, a strain of Lactobacillus johnsonii LMZ8 is separated and screened, and the preservation number of the strain of Lactobacillus johnsonii LMZ8 is GDMCC No: 66614. The lactobacillus johnsonii LMZ8 is good in acid resistance and has certain tolerance to high-concentration cholate. Experimental results show that the lactobacillus johnsonii LMZ8 can effectively inhibit the growth of various pathogenic bacteria such as salmonella typhimurium and salmonella enteritidis, especially has a prominent inhibition effect on five strains of multi-drug-resistant salmonella pullorum with no same drug resistance spectrum, can effectively prevent and treat the pullorum disease caused by salmonella pullorum, and improves the growth performance of chicks. The lactobacillus johnsonii LMZ8 shows remarkable development potential in the aspects of preventing and controlling food-borne pathogenic bacteria and preventing and treating diseases such as pullorum disease, and has a wide development prospect.

The application discloses sgRNA for detecting chicken salmonella pullorum and application of the sgRNA in RPA-CRISPR / Cas12b detection, and belongs to the technical field of biology. The sgRNA designed by taking group_3798 gene as a specific target has good sensitivity, specificity and repeatability when used for detecting chicken salmonella pullorum, does not depend on large experimental equipment, can visually display results, and provides a method for rapidly detecting chicken salmonella pullorum on site for poultry breeding.

This invention relates to the field of rapid detection technology for pathogenic microorganisms, specifically to a primer-probe combination, kit, and method of use for detecting Salmonella pullorum based on fluorescent RT-RAA. The primers for detecting Salmonella pullorum based on fluorescent RT-RAA are shown in SEQ ID NO.1 and SEQ ID NO.4. The kit developed using this primer-probe combination allows for rapid and efficient detection of Salmonella pullorum in samples using RT-RAA fluorescence, exhibiting high sensitivity and good repeatability, and showing promising application prospects in related rapid detection methods.

The invention discloses a lytic salmonella pullorum bacteriophage and application thereof.The bacteriophage is provided with a polyhedral head and a contractile tail, the average diameter of the head is about 71 nm, the average diameter of the tail is about 126 nm, the bacteriophage belongs to the muscular tail bacteriophage family, and the bacteriophage can split 15 salmonella pullorum strains in 18 salmonella clinical isolates and can be used for treating salmonella pullorum. The incubation period is about 40 minutes, the cracking period is about 170 minutes, and the cracking amount reaches 142 PFU / cell. The lytic salmonella pullorum bacteriophage, the lytic salmonella pullorum bacteriophage and the separated bacteriophage have the advantages of wide host spectrum, high splitting efficiency, low optimal infection complex number, low production cost and the like, clinical tests show that the death rate of infected chickens can be remarkably reduced, the detection rate of salmonella in visceral organs is reduced, and the application prospect is wide. The salmonella pullorum prevention and control agent can effectively prevent and control salmonella pullorum, relieve pathological injuries, purify chicken flocks after long-term use, improve the laying rate and the laying quality, and provide a novel safe, efficient, economical and environment-friendly salmonella pullorum prevention and control scheme for poultry breeding industry.

The application discloses a method for establishing a chicken white diarrhea Salmonella typhimurium challenge model and relates to the technical field of biological medicine. The method comprises the following steps: taking 12-day-old chicken embryos and injecting a chitosan oligosaccharide solution into the air chamber of the chicken embryos; placing the chicken embryos injected with the chitosan oligosaccharide solution in an incubator to incubate until the chicken embryos are 14-day-old; injecting chicken white diarrhea Salmonella typhimurium into the air chamber of the 14-day-old chicken embryos to perform challenge; placing the chicken embryos after challenge in the incubator to continue incubation until the chicken embryos are 21-day-old; and establishing a challenge model after the chicken embryos hatch into chicks at the age of 21 days. The application creatively optimizes the method for establishing a challenge model, screens the optimal injection concentration of a chitosan solution and the challenge concentration of chicken white diarrhea Salmonella typhimurium; and the chicken white diarrhea Salmonella typhimurium challenge model established by the method can control the highest morbidity and chick survival rate of chicks after 24 hours and ensure uniform disease onset time.

The application discloses a bacillus mucilaginosus for inhibiting systemic infection type, pneumonia type and neurotype chicken pullorum salmonella and application thereof. The bacillus mucilaginosus for inhibiting systemic infection type, pneumonia type and neurotype chicken pullorum salmonella is named Anti-S.gallinarum, is preserved in the China Center for Type Culture Collection, and has a preservation number of CCTCC NO: M20251300.

This invention discloses a fermented Sophora japonica flower extract for enhancing the efficacy of colistin, its preparation method, and its application. The preparation method of the fermented Sophora japonica flower extract includes the following steps: inoculating Aspergillus niger into a solid fermentation medium containing Sophora japonica flowers for fermentation to obtain a fermented product; separating the fermented product to obtain the fermented Sophora japonica flower extract. This fermented Sophora japonica flower extract can enhance the inhibitory effect of colistin on Salmonella (especially Salmonella pullorum), helping to reduce antibiotic use and combat the growth of drug resistance.