24 results about "Salmonella Pullorum" patented technology

The invention relates to the technical field of prevention and control of salmonella pullorum, in particular to novel application of lactobacillus rhamnosus P118. The lactobacillus rhamnosus P118 provided by the invention has any one of the following applications: (1) the infection rate of chicks to salmonella pullorum is reduced, (2) the infection rate of the chicks to salmonella pullorum is reduced, (3) the infection rate of the chicks to salmonella pullorum is reduced, and (4) the infection rate of the chicks to salmonella pullorum is reduced; (2) the egg laying performance of hens infected with salmonella pullorum is improved; (3) reducing the bacterial load in the tissues and organs of the hens; (4) reducing horizontal propagation and vertical propagation of salmonella pullorum; and (5) reducing the risk that salmonella pullorum is vertically spread from hens to offspring through eggs. The antagonistic effect of the lactobacillus rhamnosus P118 on pullorum disease in an in-vivo and in-vitro infection model is systematically evaluated, and it is found that the P118 has a remarkable inhibiting effect on colonization infection of the pullorum disease in chick and laying hen models in the egg laying peak period.

This invention belongs to the field of molecular biology detection technology, specifically relating to a primer and probe combination for detecting avian Salmonella based on the RAA-LFD method and its application, particularly to a primer and probe combination for detecting three avian serotypes of Salmonella: Salmonella pullorum, Salmonella enteritidis, and Salmonella typhimurium, based on the RAA-LFD method and its application. This invention designs different types of probes and primers targeting the genes of serotypes SP, SE, and STM of Salmonella. Using the primer and probe combination of this invention, rapid, sensitive, and specific detection of Salmonella serotypes and the three avian Salmonella serotypes SP, SE, and STM can be achieved, greatly shortening the detection cycle of avian salmonellosis and improving the detection efficiency and accuracy of avian salmonellosis.

The application belongs to the field of biotechnology detection, and particularly relates to a multiplex PCR detection kit for identifying and distinguishing chicken pullorum salmonella and chicken typhoid salmonella. The kit comprises stn gene detection primers, I137_14445 gene detection primers and ybgL gene detection primers. The kit can quickly, high-throughput and accurately identify and distinguish chicken pullorum salmonella and chicken typhoid salmonella, can replace traditional salmonella serological typing and biochemical identification and other complex procedures, and provides a simple, rapid, reproducible new method for monitoring and laboratory diagnosis of chicken pullorum salmonella and chicken typhoid salmonella.

The application belongs to the field of feed additives, and particularly discloses application of sodium butyrate in preparation of a feed additive against chicken white dysentery Salmonella, and sodium butyrate is added in chicken basic feed as the feed additive against chicken white dysentery Salmonella; the addition of sodium butyrate in chicken basic feed can significantly reduce the bacterial load of chicken white dysentery Salmonella infection in chicken liver and spleen, significantly increase the ratio of villus length to crypt depth of the cecum of chicken infected with chicken white dysentery Salmonella, and improve the intestinal absorption capacity; the content of CD3+ T cells, TCR+ T cells, macrophages and B cells in the liver of chicken infected with chicken white dysentery Salmonella can be significantly reduced; and then the body weight of chicken infected with chicken white dysentery Salmonella is significantly increased, and the survival rate of chicken infected with chicken white dysentery Salmonella is increased. In summary, the addition of sodium butyrate in chicken basic feed can enhance the resistance to chicken white dysentery Salmonella infection.

The invention discloses a method and a kit for simultaneously detecting avian leukosis virus, mycoplasma synoviae and salmonella pullorum antibodies, and belongs to the technical field of animal epidemic disease detection. According to the method, an avian leukosis virus p27 protein, a chicken mycoplasma synoviae p50 protein and a truncated GroEL-delta8-1 protein of a dominant antigen GroEL of salmonella pullorum are taken as coating antigens, synchronous detection of the three antibodies is realized by optimizing ELSA reaction conditions, and a kit capable of simultaneously detecting the three antibodies is developed at the same time. The kit has the characteristics of strong specificity, high sensitivity and simplicity and convenience in operation, is suitable for rapid screening and purification of poultry epidemic diseases, and reduces the detection cost.

The invention belongs to the technical field of biological detection, and discloses a bacteriophage PQ38, application thereof and a salmonella inhibiting medicine, the bacteriophage PQ38 has the preservation number of GDMCC NO: 67169-B1, is preserved in Guangdong Microbial Culture Collection Center, the preservation time is October 27, 2025, and the bacteriophage PQ38 is named as Siphoviridae jerseyvirus in taxonomy; the bacteriophage has a good salmonella splitting capability in salmonella, and tests show that the bacteriophage has an excellent capability of splitting salmonella pullorum.

The invention relates to the technical field of agricultural biology, in particular to an alpha spiral mutant of granulysin with improved antibacterial activity and application of the alpha spiral mutant. The amino acid sequence of the alpha spiral mutant peptide of granulysin with improved antibacterial activity is as shown in SEQ ID No: 2. According to the present invention, the antibacterial activity of the granulysin mutant on different bacteria such as gram-negative bacteria salmonella typhimurium, escherichia coli and salmonella pullorum is enhanced;

The invention relates to the technical field of agricultural biology, in particular to an antibacterial peptide chicken NK lysin mutant and application thereof. According to the present invention, the antibacterial activity of the chicken NK lysin mutant on different bacteria such as salmonella typhimurium 541, gram-negative bacteria, Escherichia coli O157: H7 1491, gram-negative bacteria, salmonella pullorum of gram-negative bacteria, and Staphylococcus aureus can be enhanced, and the antibacterial activity of the chicken NK lysin mutant on different bacteria such as salmonella typhimurium 541, gram-negative bacteria, Escherichia coli O157: H7 1491, gram-negative bacteria, salmonella pullorum of gram-negative bacteria, and Staphylococcus aureus can be enhanced;

The present application relates to a CNTN5 gene related to a host chicken Salmonella pullorum resistance trait and a SNP molecular marker and application thereof, the CNTN5 gene and the SNP molecular marker are used for breeding or assisting breeding of chicken breeds with high resistance to chicken Salmonella pullorum, the nucleotide sequence of the CNTN5 gene is shown as SEQ ID NO. 1, the CNTN5 gene SNP molecular marker is located in the intron region of the CNTN5 gene on chromosome 1 of the chicken, at the 185063016bp site on the chromosome 1, and the polymorphism is T or C; when the base at the CNTN5 gene SNP molecular marker is T, the chicken has high resistance to chicken Salmonella pullorum; when the base at the CNTN5 gene SNP molecular marker is C, the chicken has low resistance to chicken Salmonella pullorum. The CNTN5 gene and the SNP molecular marker provided by the present application can be used to quickly and effectively screen and breed chicken breeds with high resistance to chicken Salmonella pullorum.

This invention relates to the field of agricultural biotechnology, specifically to the antimicrobial peptide chicken NK lysin mutant and its applications. The chicken NK lysin mutant of this invention is effective against various bacteria, such as Salmonella typhimurium (…). Salmonella typhimurium 541, Gram-negative bacteria), Escherichia coli ( Escherichia coli O157:H7 1491, Gram-negative bacteria), Gram-negative bacteria Salmonella pullorum ( salmonella pullorum ) and Staphylococcus aureus ( Staphylococcus aureus The antibacterial activity of ) is enhanced.

The application discloses a chicken salmonella pullorum strain stably expressing variant O antigen and a construction method and application thereof, and constructs an expression plasmid containing a J23119 promoter and a SPUL_2404 gene, and then the expression plasmid is transferred into chicken salmonella pullorum, so that a chicken salmonella pullorum strain overexpressing the SPUL_2404 gene is constructed to stably express the variant O antigen, and agglutination test shows that the strain only has a typical agglutination reaction with chicken salmonella pullorum variant serums, and does not have a typical agglutination reaction with chicken salmonella pullorum standard serums, and after subculture, O antigens of all subclone colonies are stable, and only have a typical agglutination reaction with the variant serums, so that the strain can be used for preparing chicken salmonella pullorum variant agglutination antigens.

The invention discloses a nano-enzyme for preventing salmonella pullorum infection, the nano-enzyme comprises kaempferol and cerium ions, the kaempferol and the cerium ions form a kaempferol-cerium composite nano-enzyme CeKae NPs through coordination, and the nano-enzyme has superoxide dismutase (SOD)-like activity and catalase (CAT)-like activity. The invention further discloses a preparation method and application of the nano-enzyme for preventing salmonella pullorum infection. The CeKae NPs nano-enzyme provided by the invention comprises kaempferol and cerium ions, has efficient SOD and CAT nano-enzyme activity, and can realize antioxidant self-cascade nano-enzyme reaction, effectively remove active oxygen and improve the pathological environment.

The present application relates to the technical field of microorganism, and especially relates to a kind of Enterococcus durans C24, probiotic agent and application thereof.The present application provides Enterococcus durans C24, and its preservation number is CGMCC No.33995.The strain has broad-spectrum antibacterial activity, can inhibit Salmonella enteritidis, Salmonella pullorum and Salmonella typhimurium, has no hemolytic activity, and has good stress resistance, can survive in gastrointestinal environment, and also has high biological safety, and is expected to be used for clinical prevention and control of salmonellosis.

The invention discloses lactobacillus johnsonii for preventing and treating pullorum disease salmonellosis and application of the lactobacillus johnsonii, and belongs to the field of microorganisms. According to the invention, a strain of Lactobacillus johnsonii LMZ8 is separated and screened, and the preservation number of the strain of Lactobacillus johnsonii LMZ8 is GDMCC No: 66614. The lactobacillus johnsonii LMZ8 is good in acid resistance and has certain tolerance to high-concentration cholate. Experimental results show that the lactobacillus johnsonii LMZ8 can effectively inhibit the growth of various pathogenic bacteria such as salmonella typhimurium and salmonella enteritidis, especially has a prominent inhibition effect on five strains of multi-drug-resistant salmonella pullorum with no same drug resistance spectrum, can effectively prevent and treat the pullorum disease caused by salmonella pullorum, and improves the growth performance of chicks. The lactobacillus johnsonii LMZ8 shows remarkable development potential in the aspects of preventing and controlling food-borne pathogenic bacteria and preventing and treating diseases such as pullorum disease, and has a wide development prospect.

The application discloses sgRNA for detecting chicken salmonella pullorum and application of the sgRNA in RPA-CRISPR / Cas12b detection, and belongs to the technical field of biology. The sgRNA designed by taking group_3798 gene as a specific target has good sensitivity, specificity and repeatability when used for detecting chicken salmonella pullorum, does not depend on large experimental equipment, can visually display results, and provides a method for rapidly detecting chicken salmonella pullorum on site for poultry breeding.

This invention relates to the field of rapid detection technology for pathogenic microorganisms, specifically to a primer-probe combination, kit, and method of use for detecting Salmonella pullorum based on fluorescent RT-RAA. The primers for detecting Salmonella pullorum based on fluorescent RT-RAA are shown in SEQ ID NO.1 and SEQ ID NO.4. The kit developed using this primer-probe combination allows for rapid and efficient detection of Salmonella pullorum in samples using RT-RAA fluorescence, exhibiting high sensitivity and good repeatability, and showing promising application prospects in related rapid detection methods.

A primer and probe for detecting Salmonella pullorum, and a kit. The primer comprises SP-F and SP-R for detecting the target sequence of the Salmonella pullorum (SP) citE2 gene, and the probe is modified with a fluorescent reporter group and a fluorescent quenching group. By means of providing the primer pair of SP-F and SP-R, which pair specifically binds to the target sequence of the citE2 gene, and combining the primer pair with the probe, whic is used for specific recognition, the accurate and sensitive detection of Salmonella pullorum can be realized.

The invention discloses a lytic salmonella pullorum bacteriophage and application thereof.The bacteriophage is provided with a polyhedral head and a contractile tail, the average diameter of the head is about 71 nm, the average diameter of the tail is about 126 nm, the bacteriophage belongs to the muscular tail bacteriophage family, and the bacteriophage can split 15 salmonella pullorum strains in 18 salmonella clinical isolates and can be used for treating salmonella pullorum. The incubation period is about 40 minutes, the cracking period is about 170 minutes, and the cracking amount reaches 142 PFU / cell. The lytic salmonella pullorum bacteriophage, the lytic salmonella pullorum bacteriophage and the separated bacteriophage have the advantages of wide host spectrum, high splitting efficiency, low optimal infection complex number, low production cost and the like, clinical tests show that the death rate of infected chickens can be remarkably reduced, the detection rate of salmonella in visceral organs is reduced, and the application prospect is wide. The salmonella pullorum prevention and control agent can effectively prevent and control salmonella pullorum, relieve pathological injuries, purify chicken flocks after long-term use, improve the laying rate and the laying quality, and provide a novel safe, efficient, economical and environment-friendly salmonella pullorum prevention and control scheme for poultry breeding industry.

The invention relates to the technical field of pullorum disease, in particular to a PCR (Polymerase Chain Reaction) detection kit for quickly identifying salmonella gallinarum with pullorum disease, which comprises a detection system and a shell, and a top cover is hinged to one side of the top of the shell; a loading plate is vertically arranged in the shell in a sliding fit manner, a plurality of loading ports for loading PCR tubes are formed in the loading plate, and loading bags are fixedly connected to the loading ports; an oscillating assembly used for oscillating the loading bag and a limiting assembly used for limiting the position of the loading plate are arranged in the shell. The oscillation assembly comprises an oscillation plate, a controller and a driving part, and the controller is used for controlling the driving part to operate; the oscillating plate is located below the loading bag and is in vertical sliding fit with the inner side wall of the shell; the driving piece is fixedly connected to the inner bottom wall of the shell, and an output shaft of the driving piece is fixedly connected with a stirring wheel. Through the synergistic effect, the oscillation plate can achieve the efficient oscillation effect, it is ensured that reactants are evenly distributed in a PCR tube, and the uniformity and efficiency of the PCR reaction are improved.

The application discloses a method for establishing a chicken white diarrhea Salmonella typhimurium challenge model and relates to the technical field of biological medicine. The method comprises the following steps: taking 12-day-old chicken embryos and injecting a chitosan oligosaccharide solution into the air chamber of the chicken embryos; placing the chicken embryos injected with the chitosan oligosaccharide solution in an incubator to incubate until the chicken embryos are 14-day-old; injecting chicken white diarrhea Salmonella typhimurium into the air chamber of the 14-day-old chicken embryos to perform challenge; placing the chicken embryos after challenge in the incubator to continue incubation until the chicken embryos are 21-day-old; and establishing a challenge model after the chicken embryos hatch into chicks at the age of 21 days. The application creatively optimizes the method for establishing a challenge model, screens the optimal injection concentration of a chitosan solution and the challenge concentration of chicken white diarrhea Salmonella typhimurium; and the chicken white diarrhea Salmonella typhimurium challenge model established by the method can control the highest morbidity and chick survival rate of chicks after 24 hours and ensure uniform disease onset time.

The present application relates to the technical field of Salmonella bacteriophage, and particularly relates to a bacteriophage capable of lysing Salmonella Blegdam (Salmonella Blegdam bacteriophage) and a composition and application thereof. The bacteriophage is Salmonella bacteriophage RDP-SA-22010, the preservation number of which is CGMCC No. 45274, and the bacteriophage was preserved in the China General Microbiological Culture Collection Center on September 21, 2022. The Salmonella bacteriophage RDP-SA-22010 can also lyse Salmonella enteritidis, Salmonella pullorum and Salmonella kentucky. A composition comprises the Salmonella bacteriophage RDP-SA-22010. A reagent or kit comprises the above-mentioned composition. The present application also provides that the Salmonella bacteriophage RDP-SA-22010 can be applied to the preparation of a biological sterilizing agent, the preparation of a medicine for treating diseases caused by Salmonella and the preparation of a feed additive. The Salmonella bacteriophage RDP-SA-22010 in the present application provides a new biological control means for the prevention and treatment of Salmonella Blegdam.

The application discloses a bacillus mucilaginosus for inhibiting systemic infection type, pneumonia type and neurotype chicken pullorum salmonella and application thereof. The bacillus mucilaginosus for inhibiting systemic infection type, pneumonia type and neurotype chicken pullorum salmonella is named Anti-S.gallinarum, is preserved in the China Center for Type Culture Collection, and has a preservation number of CCTCC NO: M20251300.

The invention discloses a novel salmonella pullorum SP1910 strain, a pullorum disease antigen prepared from the novel salmonella pullorum SP1910 strain and a preparation method and application of the antigen, the strain has better stability and specificity, the detection effect of the antigen prepared from the strain on pullorum disease is superior to that of a commercial antigen, the pullorum disease can be efficiently detected, positive chickens are eliminated, and the detection cost is reduced. Chicken flocks are purified, infection of pullorum disease is reduced, and breeding benefits are improved.

This invention discloses a fermented Sophora japonica flower extract for enhancing the efficacy of colistin, its preparation method, and its application. The preparation method of the fermented Sophora japonica flower extract includes the following steps: inoculating Aspergillus niger into a solid fermentation medium containing Sophora japonica flowers for fermentation to obtain a fermented product; separating the fermented product to obtain the fermented Sophora japonica flower extract. This fermented Sophora japonica flower extract can enhance the inhibitory effect of colistin on Salmonella (especially Salmonella pullorum), helping to reduce antibiotic use and combat the growth of drug resistance.