39 results about "Salmonella Pullorum" patented technology

The present invention belongs to the field of biotechnology and relates to a five - plex fluorescence PCR detection reagent for Salmonella, Salmonella enteritidis, Salmonella typhimurium, Salmonella pullorum and Salmonella gallinarum and its application. By using the five - plex fluorescence PCR detection reagent of the present invention, the purpose of simultaneously identifying and detecting Salmonella enteritidis, Salmonella typhimurium, Salmonella pullorum and Salmonella gallinarum can be achieved. It has the advantages of simple operation, rapidity, high accuracy, high sensitivity and good repeatability, and is of great significance for the rapid and accurate detection of clinical animal salmonellosis and epidemic disease prevention and control.

The invention belongs to the technical field of molecular biological detection, and particularly relates to a primer group for detecting salmonella pullorum based on an RAA-LFD technology and application of the primer group. The invention provides a primer group for detecting salmonella pullorum based on an RAA-LFD technology. The primer group comprises a forward primer, a reverse primer and a probe, the sequence of the forward primer is as shown in SEQ ID NO. 1; the sequence of the reverse primer is as shown in SEQ ID NO. 2; the sequence of the probe is based on SEQ ID NO.3, wherein the original base G of the 31st base of the SEQ ID NO.3 is replaced by an idSp group. According to the specific primer based on the RAA-LFD technology, the phenomenon of false positive caused by generation of primer dimers is avoided, the specificity is high, and the sensitivity is high. The primer group provided by the invention can be used for detecting salmonella pullorum and related strains, and only the salmonella pullorum is positive, so that the primer group shows good specificity.

The invention relates to the technical field of prevention and control of salmonella pullorum, in particular to novel application of lactobacillus rhamnosus P118. The lactobacillus rhamnosus P118 provided by the invention has any one of the following applications: (1) the infection rate of chicks to salmonella pullorum is reduced, (2) the infection rate of the chicks to salmonella pullorum is reduced, (3) the infection rate of the chicks to salmonella pullorum is reduced, and (4) the infection rate of the chicks to salmonella pullorum is reduced; (2) the egg laying performance of hens infected with salmonella pullorum is improved; (3) reducing the bacterial load in the tissues and organs of the hens; (4) reducing horizontal propagation and vertical propagation of salmonella pullorum; and (5) reducing the risk that salmonella pullorum is vertically spread from hens to offspring through eggs. The antagonistic effect of the lactobacillus rhamnosus P118 on pullorum disease in an in-vivo and in-vitro infection model is systematically evaluated, and it is found that the P118 has a remarkable inhibiting effect on colonization infection of the pullorum disease in chick and laying hen models in the egg laying peak period.

This invention belongs to the field of molecular biology detection technology, specifically relating to a primer and probe combination for detecting avian Salmonella based on the RAA-LFD method and its application, particularly to a primer and probe combination for detecting three avian serotypes of Salmonella: Salmonella pullorum, Salmonella enteritidis, and Salmonella typhimurium, based on the RAA-LFD method and its application. This invention designs different types of probes and primers targeting the genes of serotypes SP, SE, and STM of Salmonella. Using the primer and probe combination of this invention, rapid, sensitive, and specific detection of Salmonella serotypes and the three avian Salmonella serotypes SP, SE, and STM can be achieved, greatly shortening the detection cycle of avian salmonellosis and improving the detection efficiency and accuracy of avian salmonellosis.

The application belongs to the field of biotechnology detection, and particularly relates to a multiplex PCR detection kit for identifying and distinguishing chicken pullorum salmonella and chicken typhoid salmonella. The kit comprises stn gene detection primers, I137_14445 gene detection primers and ybgL gene detection primers. The kit can quickly, high-throughput and accurately identify and distinguish chicken pullorum salmonella and chicken typhoid salmonella, can replace traditional salmonella serological typing and biochemical identification and other complex procedures, and provides a simple, rapid, reproducible new method for monitoring and laboratory diagnosis of chicken pullorum salmonella and chicken typhoid salmonella.

The invention discloses a method for establishing a salmonella pullorum challenge model, and relates to the technical field of biological medicines. The invention discloses a method for establishing a salmonella pullorum challenge model. The method comprises the following steps: taking a 12-day-old chick embryo, and injecting a chitosan oligosaccharide solution into a chick embryo air chamber; placing the chick embryos injected with the chitosan oligosaccharide solution into an incubator for incubation until the chick embryos are 14 days old; salmonella pullorum is injected into an air chamber of the 14-day-old chick embryo for challenging; after challenging the chick embryos, continuously incubating the chick embryos in an incubator until 21 days old; and after the chick embryos are incubated to 21 days old, hatched and changed into chicks, establishing a toxic attack model. According to the invention, the method of the challenge model is creatively optimized, and the optimal chitosan solution injection concentration and the optimal challenge concentration of salmonella pullorum are screened; the salmonella pullorum challenge model is built through the method, the highest morbidity and survival rate of chicks can be controlled after 24 h, and it can be guaranteed that the morbidity time is uniform.

The invention discloses a method for rapidly detecting salmonella pullorum by fluorescent quantitative PCR (polymerase chain reaction) and used primers, and the method for rapidly detecting salmonella pullorum by fluorescent quantitative PCR specifically comprises the following steps: (1) collecting a sample; (2) nucleic acid extraction; (3) preparing a reaction system; (4) running a sample detection program; and (5) detecting a test result and judging. According to the rapid detection method disclosed by the invention, an ultra-short-range PCR program is designed as follows: pre-denaturation is performed at 95 DEG C for 2 minutes; according to the present invention, with the program setting, the salmonella pullorum detection time is shortened by about 60% compared with the salmonella pullorum detection time of the commercially available kit and the commercial standard program, and the detection efficiency is substantially improved; the cost of a single detection reagent is reduced by 40% compared with that of an imported kit, and the economic cost is reduced; compared with a traditional culture method, the method has the advantages that the time is 3-5 days earlier, the detection result is reliable, the repeatability is good, and the method can be used for clinical diagnosis and monitoring of SP.

The invention provides a polysaccharide antigen derived from salmonella pullorum and marked by fluorescent molecules as well as a preparation method and application of the polysaccharide antigen. The invention provides a simple, convenient and rapid extraction method of the salmonella pullorum polysaccharide antigen, and the polysaccharide antigen for bacterial serological detection is prepared by combining fluorescence labeling on the basis of the experimental principle of fluorescence polarization. The polysaccharide antigen prepared according to the method provided by the invention can be used for serological detection of salmonella pullorum and has good sensitivity, and a new method is provided for detection of salmonella pullorum infection.

The application belongs to the field of feed additives, and particularly discloses application of sodium butyrate in preparation of a feed additive against chicken white dysentery Salmonella, and sodium butyrate is added in chicken basic feed as the feed additive against chicken white dysentery Salmonella; the addition of sodium butyrate in chicken basic feed can significantly reduce the bacterial load of chicken white dysentery Salmonella infection in chicken liver and spleen, significantly increase the ratio of villus length to crypt depth of the cecum of chicken infected with chicken white dysentery Salmonella, and improve the intestinal absorption capacity; the content of CD3+ T cells, TCR+ T cells, macrophages and B cells in the liver of chicken infected with chicken white dysentery Salmonella can be significantly reduced; and then the body weight of chicken infected with chicken white dysentery Salmonella is significantly increased, and the survival rate of chicken infected with chicken white dysentery Salmonella is increased. In summary, the addition of sodium butyrate in chicken basic feed can enhance the resistance to chicken white dysentery Salmonella infection.

The invention discloses a method and a kit for simultaneously detecting avian leukosis virus, mycoplasma synoviae and salmonella pullorum antibodies, and belongs to the technical field of animal epidemic disease detection. According to the method, an avian leukosis virus p27 protein, a chicken mycoplasma synoviae p50 protein and a truncated GroEL-delta8-1 protein of a dominant antigen GroEL of salmonella pullorum are taken as coating antigens, synchronous detection of the three antibodies is realized by optimizing ELSA reaction conditions, and a kit capable of simultaneously detecting the three antibodies is developed at the same time. The kit has the characteristics of strong specificity, high sensitivity and simplicity and convenience in operation, is suitable for rapid screening and purification of poultry epidemic diseases, and reduces the detection cost.

The present invention discloses a high-temperature resistant Salmonella pullorum phage, a phage composition thereof and its application. The high-temperature resistant Salmonella pullorum phage is named PC127 and was deposited in the General Microbiology Center of the China Microorganism Culture Collection Administration on April 12, 2021, with a deposit number of CGMCC No. 22366. The phage composition includes phage PC127 and other matching phages. The phage has strong lysis and a wide lysis spectrum against Salmonella pullorum, can effectively prevent and treat pullorum, and has the effects of reducing the mortality rate of chickens, reducing the positive rate of pullorum antibodies, and increasing egg production. In addition, it can be used as an active ingredient and detection kit of an environmental disinfectant. While solving Salmonella infection, long-term application can purify pullorum in chicken farms.

The invention belongs to the technical field of biological detection, and discloses a bacteriophage PQ38, application thereof and a salmonella inhibiting medicine, the bacteriophage PQ38 has the preservation number of GDMCC NO: 67169-B1, is preserved in Guangdong Microbial Culture Collection Center, the preservation time is October 27, 2025, and the bacteriophage PQ38 is named as Siphoviridae jerseyvirus in taxonomy; the bacteriophage has a good salmonella splitting capability in salmonella, and tests show that the bacteriophage has an excellent capability of splitting salmonella pullorum.

The invention relates to the technical field of agricultural biology, in particular to an alpha spiral mutant of granulysin with improved antibacterial activity and application of the alpha spiral mutant. The amino acid sequence of the alpha spiral mutant peptide of granulysin with improved antibacterial activity is as shown in SEQ ID No: 2. According to the present invention, the antibacterial activity of the granulysin mutant on different bacteria such as gram-negative bacteria salmonella typhimurium, escherichia coli and salmonella pullorum is enhanced;

The invention relates to the technical field of agricultural biology, in particular to an antibacterial peptide chicken NK lysin mutant and application thereof. According to the present invention, the antibacterial activity of the chicken NK lysin mutant on different bacteria such as salmonella typhimurium 541, gram-negative bacteria, Escherichia coli O157: H7 1491, gram-negative bacteria, salmonella pullorum of gram-negative bacteria, and Staphylococcus aureus can be enhanced, and the antibacterial activity of the chicken NK lysin mutant on different bacteria such as salmonella typhimurium 541, gram-negative bacteria, Escherichia coli O157: H7 1491, gram-negative bacteria, salmonella pullorum of gram-negative bacteria, and Staphylococcus aureus can be enhanced;

The present application relates to a CNTN5 gene related to a host chicken Salmonella pullorum resistance trait and a SNP molecular marker and application thereof, the CNTN5 gene and the SNP molecular marker are used for breeding or assisting breeding of chicken breeds with high resistance to chicken Salmonella pullorum, the nucleotide sequence of the CNTN5 gene is shown as SEQ ID NO. 1, the CNTN5 gene SNP molecular marker is located in the intron region of the CNTN5 gene on chromosome 1 of the chicken, at the 185063016bp site on the chromosome 1, and the polymorphism is T or C; when the base at the CNTN5 gene SNP molecular marker is T, the chicken has high resistance to chicken Salmonella pullorum; when the base at the CNTN5 gene SNP molecular marker is C, the chicken has low resistance to chicken Salmonella pullorum. The CNTN5 gene and the SNP molecular marker provided by the present application can be used to quickly and effectively screen and breed chicken breeds with high resistance to chicken Salmonella pullorum.

This invention relates to the field of agricultural biotechnology, specifically to the antimicrobial peptide chicken NK lysin mutant and its applications. The chicken NK lysin mutant of this invention is effective against various bacteria, such as Salmonella typhimurium (…). Salmonella typhimurium 541, Gram-negative bacteria), Escherichia coli ( Escherichia coli O157:H7 1491, Gram-negative bacteria), Gram-negative bacteria Salmonella pullorum ( salmonella pullorum ) and Staphylococcus aureus ( Staphylococcus aureus The antibacterial activity of ) is enhanced.

The invention relates to the technical field of bacterial detection, in particular to a Salmonella pullorum high-throughput detection method which comprises the following steps: inoculating Salmonella pullorum to a TSB culture medium, culturing to obtain a bacterial liquid, inactivating the bacterial liquid, centrifuging to remove supernatant, dyeing by using a crystal violet ethanol solution, adjusting the concentration of the Salmonella pullorum to 5 * 10 < 7 > CFU / mL to 5 * 10 < 9 > CFU / mL, and carrying out high-throughput detection on the Salmonella pullorum. And preparing to obtain a salmonella gallinarum antigen solution. The method comprises the following steps: diluting serum to be detected, adding into a porous plate, drying and fixing, sealing by using a PBS (Phosphate Buffer Solution), adding a salmonella gallinarum antigen solution, incubating, washing, and observing by naked eyes or measuring by using a microplate reader so as to judge the result. According to the invention, high-flux, low-cost and rapid detection of pullorum disease can be realized, the problem of insufficient accuracy of traditional manual interpretation is solved, and a technical method is provided for identification and diagnosis of pullorum disease.

The invention discloses a method for rapidly extracting and enriching salmonella pullorum nucleic acid based on chitosan modified diatomite. The method comprises the following steps: (1) pretreating diatomite; (2) functional modification of chitosan; and (3) extracting salmonella pullorum nucleic acid. The chitosan-diatomite composite material disclosed by the invention is based on a synergistic mechanism of an electrostatic interaction micropore interception effect, the selectivity is improved by utilizing the cationic characteristic of chitosan, the chitosan is fixed on the surface of diatomite through glutaraldehyde crosslinking, the dissolution loss in an acid environment is avoided, the high specific surface area of the diatomite and the charge adsorption of the chitosan are combined, and the adsorption capacity of the diatomite is improved. A'physical interception + chemical bonding 'dual-mode enrichment mechanism is established, efficient capture of existing nucleic acid is achieved, qPCR detection sensitivity is remarkably improved, the bottleneck problem of a traditional nucleic acid extraction method in complex poultry samples is solved, and the method has the advantages of high detection rate, high anti-interference performance and operation convenience and is suitable for popularization and application. And reliable technical support is provided for rapid molecular diagnosis and epidemiological monitoring of the pullorum disease bacteria.

The invention provides a primer probe combination, a kit and a method for detecting salmonella pullorum, and belongs to the technical field of rapid detection of pathogenic microorganisms. A series of primer probes are designed aiming at the ipaJ gene of the salmonella pullorum strain, a primer probe combination with the best amplification effect is screened from the primer probes, and the salmonella pullorum is detected by utilizing the primer probe combination and adopting a fluorescent RAA method. The detection efficiency, sensitivity (the lowest detection limit is 1.5 * 10 <-1 > pg / mu L) and accuracy of the detection method are higher than those of common PCR, and rapid specific detection of salmonella pullorum can be achieved.

The application discloses a chicken salmonella pullorum strain stably expressing variant O antigen and a construction method and application thereof, and constructs an expression plasmid containing a J23119 promoter and a SPUL_2404 gene, and then the expression plasmid is transferred into chicken salmonella pullorum, so that a chicken salmonella pullorum strain overexpressing the SPUL_2404 gene is constructed to stably express the variant O antigen, and agglutination test shows that the strain only has a typical agglutination reaction with chicken salmonella pullorum variant serums, and does not have a typical agglutination reaction with chicken salmonella pullorum standard serums, and after subculture, O antigens of all subclone colonies are stable, and only have a typical agglutination reaction with the variant serums, so that the strain can be used for preparing chicken salmonella pullorum variant agglutination antigens.

The invention discloses a nano-enzyme for preventing salmonella pullorum infection, the nano-enzyme comprises kaempferol and cerium ions, the kaempferol and the cerium ions form a kaempferol-cerium composite nano-enzyme CeKae NPs through coordination, and the nano-enzyme has superoxide dismutase (SOD)-like activity and catalase (CAT)-like activity. The invention further discloses a preparation method and application of the nano-enzyme for preventing salmonella pullorum infection. The CeKae NPs nano-enzyme provided by the invention comprises kaempferol and cerium ions, has efficient SOD and CAT nano-enzyme activity, and can realize antioxidant self-cascade nano-enzyme reaction, effectively remove active oxygen and improve the pathological environment.

The invention relates to the technical field of salmonella bacteriophages, and in particular relates to a bacteriophage capable of splitting salmonella brassicae as well as a composition and application of the bacteriophage capable of splitting the salmonella brassicae. The bacteriophage is salmonella bacteriophage RDP-SA-22010, the preservation number of the bacteriophage is CGMCC (China General Microbiological Culture Collection Center) No.45274, and the bacteriophage is preserved in the China General Microbiological Culture Collection Center on September 21, 2022. The salmonella bacteriophage RDP-SA-22010 can also be used for cracking salmonella enteritidis, salmonella pullorum and salmonella kafenkii. The invention also relates to a composition, which comprises the salmonella bacteriophage RDP-SA-22010. The reagent or the kit comprises the composition. The invention also provides application of the salmonella bacteriophage RDP-SA-22010 to preparation of a biological bactericide, preparation of a medicine for treating diseases caused by salmonella and preparation of a feed additive. The salmonella bacteriophage RDP-SA-22010 disclosed by the invention provides a novel biological prevention and treatment means for the prevention and treatment of salmonella bulidan.

The present invention discloses a rapid identification kit for Salmonella pullorum and Salmonella typhi based on res gene. The kit comprises detection primers, positive control, negative control and blank control, the positive control is Salmonella pullorum ATCC9184 genomic DNA, the negative control is Salmonella pullorum ATCC10398 genomic DNA, the blank control is sterilized double distilled water, and the primers comprise: For: 5'-ATCTCATCAACCAGCATACT-3'; Rev: 5'-TTCTTACGCAGCAATGAG-3'. The present invention also discloses a PCR method for rapid identification of two biotypes of Salmonella pullorum and Salmonella typhi serotypes in Salmonella typhi serotypes using res gene as detection target. The method of the present invention has the advantages of being fast, simple, highly specific and highly sensitive. Compared with the traditional biochemical identification method of Salmonella pullorum and Salmonella typhi, it has great advantages in detection time and detection cost, and is suitable for clinical batch detection.

The invention discloses a method for preparing a highly specific Salmonella pullorum plate agglutination antigen. The Salmonella pullorum C79-1 and C79-7 strains are revived and prepared into seed liquid, inoculated with a suitable culture medium, fermented and cultured according to a specific process, inactivated and centrifuged to collect the bacteria, and then washed at 121°C for 2 hours and multiple times to remove the protein antigen components that easily cross-react with Escherichia coli or other Enterobacteriaceae bacteria commonly found in the environment. The C79-1 and C79-7 strains are adjusted to a suitable bacterial concentration, Tween-20, crystal violet and glycerol are added, and the Salmonella pullorum plate agglutination antigen is obtained by sufficient homogenization. The antigen has higher specificity and is more accurate when used for pullorum purification detection. The bacterial yield according to the fermentation process is 4 times that of ordinary fermentation and 2 times that of solid culture. No ethanol precipitation is required, and the production cost is lower. Tween-20 is added to increase the surface tension of the antigen, solve the problem of difficulty in coating evenly during use, and provide a new and better diagnostic reagent for the purification of pullorum.

The present application relates to the technical field of microorganism, and especially relates to a kind of Enterococcus durans C24, probiotic agent and application thereof.The present application provides Enterococcus durans C24, and its preservation number is CGMCC No.33995.The strain has broad-spectrum antibacterial activity, can inhibit Salmonella enteritidis, Salmonella pullorum and Salmonella typhimurium, has no hemolytic activity, and has good stress resistance, can survive in gastrointestinal environment, and also has high biological safety, and is expected to be used for clinical prevention and control of salmonellosis.

The invention discloses lactobacillus johnsonii for preventing and treating pullorum disease salmonellosis and application of the lactobacillus johnsonii, and belongs to the field of microorganisms. According to the invention, a strain of Lactobacillus johnsonii LMZ8 is separated and screened, and the preservation number of the strain of Lactobacillus johnsonii LMZ8 is GDMCC No: 66614. The lactobacillus johnsonii LMZ8 is good in acid resistance and has certain tolerance to high-concentration cholate. Experimental results show that the lactobacillus johnsonii LMZ8 can effectively inhibit the growth of various pathogenic bacteria such as salmonella typhimurium and salmonella enteritidis, especially has a prominent inhibition effect on five strains of multi-drug-resistant salmonella pullorum with no same drug resistance spectrum, can effectively prevent and treat the pullorum disease caused by salmonella pullorum, and improves the growth performance of chicks. The lactobacillus johnsonii LMZ8 shows remarkable development potential in the aspects of preventing and controlling food-borne pathogenic bacteria and preventing and treating diseases such as pullorum disease, and has a wide development prospect.

The application discloses sgRNA for detecting chicken salmonella pullorum and application of the sgRNA in RPA-CRISPR / Cas12b detection, and belongs to the technical field of biology. The sgRNA designed by taking group_3798 gene as a specific target has good sensitivity, specificity and repeatability when used for detecting chicken salmonella pullorum, does not depend on large experimental equipment, can visually display results, and provides a method for rapidly detecting chicken salmonella pullorum on site for poultry breeding.

This invention relates to the field of rapid detection technology for pathogenic microorganisms, specifically to a primer-probe combination, kit, and method of use for detecting Salmonella pullorum based on fluorescent RT-RAA. The primers for detecting Salmonella pullorum based on fluorescent RT-RAA are shown in SEQ ID NO.1 and SEQ ID NO.4. The kit developed using this primer-probe combination allows for rapid and efficient detection of Salmonella pullorum in samples using RT-RAA fluorescence, exhibiting high sensitivity and good repeatability, and showing promising application prospects in related rapid detection methods.

A primer and probe for detecting Salmonella pullorum, and a kit. The primer comprises SP-F and SP-R for detecting the target sequence of the Salmonella pullorum (SP) citE2 gene, and the probe is modified with a fluorescent reporter group and a fluorescent quenching group. By means of providing the primer pair of SP-F and SP-R, which pair specifically binds to the target sequence of the citE2 gene, and combining the primer pair with the probe, whic is used for specific recognition, the accurate and sensitive detection of Salmonella pullorum can be realized.

The invention discloses a lytic salmonella pullorum bacteriophage and application thereof.The bacteriophage is provided with a polyhedral head and a contractile tail, the average diameter of the head is about 71 nm, the average diameter of the tail is about 126 nm, the bacteriophage belongs to the muscular tail bacteriophage family, and the bacteriophage can split 15 salmonella pullorum strains in 18 salmonella clinical isolates and can be used for treating salmonella pullorum. The incubation period is about 40 minutes, the cracking period is about 170 minutes, and the cracking amount reaches 142 PFU / cell. The lytic salmonella pullorum bacteriophage, the lytic salmonella pullorum bacteriophage and the separated bacteriophage have the advantages of wide host spectrum, high splitting efficiency, low optimal infection complex number, low production cost and the like, clinical tests show that the death rate of infected chickens can be remarkably reduced, the detection rate of salmonella in visceral organs is reduced, and the application prospect is wide. The salmonella pullorum prevention and control agent can effectively prevent and control salmonella pullorum, relieve pathological injuries, purify chicken flocks after long-term use, improve the laying rate and the laying quality, and provide a novel safe, efficient, economical and environment-friendly salmonella pullorum prevention and control scheme for poultry breeding industry.