The invention relates to specific nucleotides for
Vibrio cholerae O-antigens and applications of the specific nucleotides. The nucleotides comprise 1), at least one of nucleotides represented in SEQ ID NO: 1-8; 2), at least one of nucleotides complemented with the nucleotides represented in SEQ ID NO:1-8. The nucleotides can be used for preparing a PCR (
polymerase chain reaction) kit and a
gene chip for detecting
Vibrio cholerae. The specific nucleotides for
Vibrio cholerae O-antigens as well as the PCR kit and the
gene chip containing the nucleotides have high practicability, a preparation method of the PCR kit is simple and convenient, the detection period is short, the speed is high, the
operability is high, industrial production is facilitated, and the detection cost is relatively lower; the accuracy and sensitivity are high.