39 results about "O-Antigens" patented technology

Compositions and methods are described for vaccinating against E. coli intra-abdominal infections. The compositions contain a FimH polypeptide, one or more conjugates containing E. coli O-antigens polysaccharide covalently coupled to a carrier protein, and an adjuvant.

The invention provides a nucleotide specific for Escherichia coli 054 O-antigen, which is the total nucleotide sequence of the gene cluster for controlling the synthesis of O-antigens in Escherichia 054, e.g. isolated nucleotide represented by SEQ ID No:1, with overall length of 14062 bases, or nucleotide of SEQ ID No:1 including one or more inserted, deleted or substituted bases and sustains the isolated nucleotide functions, it also includes the oligonucleotides of wzx gines in the O-antigen gene cluster originating from the Escherichia 054, and genes and wzy genes having similar functions with wzx, and genes having similar functions with wzy genes or wzy genes. The invention proves the high specifity of oligonucleotides for Escherichia 054 O-antigen through PCR. A method for detecting and identifying Escherichia 054 in human body and the environment by means of the oligonucleotide according to the invention is also disclosed.

The invention provides a nucleotide specific for shigella bodyii 10 O-antigen, which is the total nucleotide sequence of the gene cluster for controlling the synthesis of O-antigens in Escherichia, e.g. isolated nucleotide represented by SEQ ID No:1, with overall length of 15253 bases, or nucleotide of SEQ ID No:1 including one or more inserted, deleted or substituted bases and sustaining the functions of the isolated nucleotides, it also includes the oligonucleotides of glycosyl transferase genes and oligosaccharide unit treatment genes in the O-antigen gene cluster originated from Shigella dysenteriae 10. The invention proves the high specifity of oligonucleotides for Shigella dysenteriae 10 O-antigen through PCR. A method for detecting and identifying Shigella dysenteriae 10 by means of the oligonucleotide according to the invention is also disclosed.

The present invention relates to method of testing a sample for the presence of nucleic acids encoding E. coli polysaccharide O-antigen serotype O111, S. enterica polysaccharide O-antigen serotype C2 and S. enterica polysaccharide O-antigen serotype B.

Compositions and methods are described for vaccinating against E. coli urinary tract infections. The compositions contain a FimH polypeptide, one or more conjugates containing E. coli O-antigens polysaccharide covalently coupled to a carrier protein, and an adjuvant.

The invention relates to a specific nucleotide for aeromonas hydrophila O antigens and application thereof. The nucleotide comprises: (1) at least one of nucleotides shown as SEQ ID NO:1 to SEQ ID NO:8; (2) at least one of nucleotides which are complementary with the nucleotides shown as SEQ ID NO:1 to SEQ ID NO:8. The nucleotides can be used for preparing a PCR (Polymerase Chain Reaction) kit and a gene chip, which are used for detecting aeromonas hydrophila. The specific nucleotide for theaeromonas hydrophila O antigens and the PCR kit and the gene chip, which contain the nucleotide, have high practicability; the PCR kit has the advantages of simple and convenient preparation method, short detection period, rapid speed and high operability; the industrial production is facilitated and the detection cost is relatively lower; the accuracy is high and the sensitivity is high.

The invention discloses a vibrio parahaemolyticus bacteriophage vB_VpP_DE17, which has a strong lysis effect on vibrio parahaemolyticus and can infect 10 vibrio parahaemolyticus in 11 O antigens. The bacteriophage can be amplified through a specific host to generate a fermentation product with high titer, and can tolerate the high temperature of 60 DEG C and tolerate pH of 5.0-10.0. The optimal multiplicity of infection (MOI) of the vibrio parahaemolyticus bacteriophage is wide, and the vibrio parahaemolyticus bacteriophage has a strong effect of preventing and controlling the vibrio parahaemolyticus when the MOI is in a range of 1-0.1. Therefore, the vibrio parahaemolyticus bacteriophage can be used for preventing and controlling acute hepatopancreas necrosis syndrome caused by vibrio parahaemolyticus in the aquaculture process, and has high application value.

The invention provides a nucleotide specific for Escherichia coli 0145 O-antigen, which is the total nucleotide sequence of the gene cluster for controlling the synthesis of O-antigens in Escherichia, e.g. isolated nucleotide represented by SEQ ID No:1, with overall length of 16932 bases, or nucleotide of SEQ ID No:1 including one or more inserted, deleted or substituted bases and sustaining the functions of the isolated nucleotides, it also includes the oligonucleotides of glycosyl transferase genes and oligosaccharide unit treatment genes in the O-antigen gene cluster originated from Escherichia 0145. The invention proves the high specifity of oligonucleotides for Escherichia coli 0145 O-antigen through PCR. A method for detecting and identifying Escherichia 0145 by means of the oligonucleotide according to the invention is also disclosed.

The invention provides a nucleotide specific for shigella bodyii 10 O-antigen, which is the total nucleotide sequence of the gene cluster for controlling the synthesis of O-antigens in shigella bodyii 10, e.g. isolated nucleotide represented by SEQ ID No:1, with overall length of 13402 bases, or nucleotide of SEQ ID No:1 including one or more inserted, deleted or substituted bases and sustains the isolated nucleotide functions, it also includes the oligonucleotides of wzx gines in the O-antigen gene cluster originating from the shigella bodyii 10, and genes and wzy genes having similar functions with wzx, and genes having similar functions with wzy genes or wzy genes or orf 8 genes with unknown functions. The invention proves the high specifity of oligonucleotides for shigella bodyii 10 O-antigen through PCR. A method for detecting and identifying shigella bodyii 10 in human body and the environment by means of the oligonucleotide according to the invention is also disclosed.

The present disclosure generally relates to genetic engineering of bacteria. More particularly, the present disclosure describes genetic engineering of E. coli to create mutant O-antigen ligase, as well as novel lipopolysaccharide molecules resulting from that genetic engineering. Methods for using those novel molecules are also described.

The invention provides a nucleotide specific for Escherichia coli 0123 O-antigen, which is the total nucleotide sequence of the gene cluster for controlling the synthesis of O-antigens in Escherichia, e.g. isolated nucleotide represented by SEQ ID No:1, with overall length of 17084 bases, or nucleotide of SEQ ID No:1 including one or more inserted, deleted or substituted bases, and sustaining the functions of the isolated nucleotides, it also includes the oligonucleotides of glycosyl transferase genes and oligosaccharide unit treatment genes in the O-antigen gene cluster originated from Escherichia 0123.

The invention provides a nucleotide specific for shigella bodyii 10 O-antigen, which is the total nucleotide sequence of the gene cluster for controlling the synthesis of O-antigens in Escherichia, e.g. isolated nucleotide represented by SEQ ID No:1, with overall length of 15253 bases, or nucleotide of SEQ ID No:1 including one or more inserted, deleted or substituted bases and sustaining the functions of the isolated nucleotides, it also includes the oligonucleotides of glycosyl transferase genes and oligosaccharide unit treatment genes in the O-antigen gene cluster originated from Shigella dysenteriae 10. The invention proves the high specifity of oligonucleotides for Shigella dysenteriae 10 O-antigen through PCR. A method for detecting and identifying Shigella dysenteriae 10 by means of the oligonucleotide according to the invention is also disclosed.

The invention provides a nucleotide specific for Escherichia coli 0141 O-antigen, which is the total nucleotide sequence of the gene cluster for controlling the synthesis of O-antigens in Escherichia 0141, e.g. isolated nucleotide represented by SEQ ID No:1, with overall length of 15601 bases, or nucleotide of SEQ ID No:1 including one or more inserted, deleted or substituted bases and sustains the isolated nucleotide functions, it also includes the oligonucleotides of glycosyl transferase genes and oligosaccharide unit treatment genes in the O-antigen gene cluster originated from Escherichia coli 0141. The invention proves the high specifity of oligonucleotides for Escherichia coli 0141 O-antigen through PCR. A method for detecting and identifying Escherichia coli 0141 in human body and the environment by means of the oligonucleotide according to the invention is also disclosed.

The invention provides a nucleotide specific for Escherichia coli 054 O-antigen, which is the total nucleotide sequence of the gene cluster for controlling the synthesis of O-antigens in Escherichia 054, e.g. isolated nucleotide represented by SEQ ID No:1, with overall length of 14062 bases, or nucleotide of SEQ ID No:1 including one or more inserted, deleted or substituted bases and sustains the isolated nucleotide functions, it also includes the oligonucleotides of wzx gines in the O-antigen gene cluster originating from the Escherichia 054, and genes and wzy genes having similar functions with wzx, and genes having similar functions with wzy genes or wzy genes. The invention proves the high specifity of oligonucleotides for Escherichia 054 O-antigen through PCR. A method for detecting and identifying Escherichia 054 in human body and the environment by means of the oligonucleotide according to the invention is also disclosed.

The invention relates to a liquid chip detection method for serotypes of four main pathogenic O antigens of yersinia enterocolon and an application thereof. According to the invention, an O3 type wbbUgene, an O5 type wzt gene, an O8 type wzy gene and an O9 type per gene of yersinia enterocolon are used as target genes, specific primers and probes for each serotype are designed and screened, and acorresponding liquid chip detection method is established. By utilizing the method provided by the invention, molecular biological detection can be accurately, quickly and sensitively carried out onfour main pathogenic O antigen serotype strains of yersinia enterocolon.

The invention provides a nucleotide specific to O-antigens of Escherichia coli O140, a full sequence of gene cluster in Escherichia coli O140, controlling O-antigen synthesis, such as the separated nucleotide shown in SEQ IN No.1, with a overall length of 14009 basic groups; or a nucleotide in SEQ IN No.1 having one or many inserted, deleted or substituted basic groups and simultaneously maintaining functions of the separated nucleotide; also including an oligonucleotide coming from glycosyltransferase gene and oligosaccharide unit-processing gene in the O-antigen gene cluster of Escherichia coli O140; by PCR, the invention verifies that oligonucleotide has specificity to all the O-antigens of Escherichia coli O140; the invention also discloses a method of using the oligonucleotide to detect and identify Escherichia coli O140 in the human body and environment.

The invention provides a nucleotide specific to O-antigens of Escherichia coli O154, a full sequence of gene cluster in Escherichia coli O154, controlling O-antigen synthesis, such as the separated nucleotide shown in SEQ IN No.1, with a overall length of 13635 basic groups; or a nucleotide in SEQ IN No.1 having one or many inserted, deleted or substituted basic groups and simultaneously maintaining functions of the separated nucleotide; also including an oligonucleotide coming from glycosyltransferase gene and oligosaccharide unit-processing gene in the O-antigen gene cluster of Escherichia coli O154; by PCR, the invention verifies that oligonucleotide has specificity to all the O-antigens of Escherichia coli O154; the invention also discloses a method of using the oligonucleotide to detect and identify Escherichia coli O154 in the human body and environment.

The invention provides a nucleotide specific for Escherichia coli 0151 O-antigen, which is the total nucleotide sequence of the gene cluster for controlling the synthesis of O-antigens in Escherichia 0151, e.g. isolated nucleotide represented by SEQ ID No:1, with overall length of 15670 bases, or nucleotide of SEQ ID No:1 including one or more inserted, deleted or substituted bases and sustains the isolated nucleotide functions, it also includes the oligonucleotides of glycosyl transferase genes and oligosaccharide unit treatment genes in the O-antigen gene cluster originated from Escherichia coli 0151. The invention proves the high specifity of oligonucleotides for Escherichia 0151 O-antigen through PCR. A method for detecting and identifying Escherichia coli 0151 in human body and the environment by means of the oligonucleotide according to the invention is also disclosed.

The invention provides a nucleotide specific for Escherichia coli 0151 O-antigen, which is the total nucleotide sequence of the gene cluster for controlling the synthesis of O-antigens in Escherichia 0151, e.g. isolated nucleotide represented by SEQ ID No:1, with overall length of 15670 bases, or nucleotide of SEQ ID No:1 including one or more inserted, deleted or substituted bases and sustains the isolated nucleotide functions, it also includes the oligonucleotides of glycosyl transferase genes and oligosaccharide unit treatment genes in the O-antigen gene cluster originated from Escherichia coli 0151. The invention proves the high specifity of oligonucleotides for Escherichia 0151 O-antigen through PCR. A method for detecting and identifying Escherichia coli 0151 in human body and the environment by means of the oligonucleotide according to the invention is also disclosed.

The invention provides a nucleotide specific for shigella bodyii 10 O-antigen, which is the total nucleotide sequence of the gene cluster for controlling the synthesis of O-antigens in shigella bodyii 10, e.g. isolated nucleotide represented by SEQ ID No:1, with overall length of 13402 bases, or nucleotide of SEQ ID No:1 including one or more inserted, deleted or substituted bases and sustains the isolated nucleotide functions, it also includes the oligonucleotides of wzx gines in the O-antigen gene cluster originating from the shigella bodyii 10, and genes and wzy genes having similar functions with wzx, and genes having similar functions with wzy genes or wzy genes or orf 8 genes with unknown functions. The invention proves the high specifity of oligonucleotides for shigella bodyii 10 O-antigen through PCR. A method for detecting and identifying shigella bodyii 10 in human body and the environment by means of the oligonucleotide according to the invention is also disclosed.

The invention relates to specific nucleotides for Vibrio cholerae O-antigens and applications of the specific nucleotides. The nucleotides comprise 1), at least one of nucleotides represented in SEQ ID NO: 1-8; 2), at least one of nucleotides complemented with the nucleotides represented in SEQ ID NO:1-8. The nucleotides can be used for preparing a PCR (polymerase chain reaction) kit and a gene chip for detecting Vibrio cholerae. The specific nucleotides for Vibrio cholerae O-antigens as well as the PCR kit and the gene chip containing the nucleotides have high practicability, a preparation method of the PCR kit is simple and convenient, the detection period is short, the speed is high, the operability is high, industrial production is facilitated, and the detection cost is relatively lower; the accuracy and sensitivity are high.

The invention relates to a nucleotide specific to Aeromonas hydrophila O antigens and an application thereof. The nucleotide comprises: (1) at least one of nucleotides shown as SEQ ID NO:1 to 8; and (2) at least one of nucleotides being complementary with the nucleotides shown as SEQ ID NO:1 to 8. The nucleotides can be used for preparing a PCR (Polymerase Chain Reaction) kit for detecting Aeromonas hydrophila and a gene chip. The nucleotide specific to the Aeromonas hydrophila O antigens and the PCR kit and the gene chip including the nucleotide have high practicability. The PCR kit has the advantages of easiness and convenience in preparation, short detection period, high speed, high operability, easiness for industrial production, relatively low detection cost, high accuracy and high sensitivity.

The invention provides a nucleotide specific for Escherichia coli 0156 O-antigen, which is the total nucleotide sequence of the gene cluster for controlling the synthesis of O-antigens in Escherichia coli 0156, e.g. isolated nucleotide represented by SEQ ID No:1, with overall length of 15390 bases, or nucleotide of SEQ ID No:1 including one or more inserted, deleted or substituted bases and sustains the isolated nucleotide functions, it also includes the oligonucleotides of glycosyl transferase genes and oligosaccharide unit treatment genes in the O-antigen gene cluster originated from Escherichia coli 0156. The invention proves the high specifity of oligonucleotides for Escherichia coli 0156 O-antigen through PCR. A method for detecting and identifying Escherichia coli 0156 in human body and the environment by means of the oligonucleotide according to the invention is also disclosed.

The application discloses a chicken salmonella pullorum strain stably expressing variant O antigen and a construction method and application thereof, and constructs an expression plasmid containing a J23119 promoter and a SPUL_2404 gene, and then the expression plasmid is transferred into chicken salmonella pullorum, so that a chicken salmonella pullorum strain overexpressing the SPUL_2404 gene is constructed to stably express the variant O antigen, and agglutination test shows that the strain only has a typical agglutination reaction with chicken salmonella pullorum variant serums, and does not have a typical agglutination reaction with chicken salmonella pullorum standard serums, and after subculture, O antigens of all subclone colonies are stable, and only have a typical agglutination reaction with the variant serums, so that the strain can be used for preparing chicken salmonella pullorum variant agglutination antigens.

The invention relates to a specific nucleotide for aeromonas hydrophila O antigens and application thereof. The nucleotide comprises: (1) at least one of nucleotides shown as SEQ ID NO:1 to SEQ ID NO:8; (2) at least one of nucleotides which are complementary with the nucleotides shown as SEQ ID NO:1 to SEQ ID NO:8. The nucleotides can be used for preparing a PCR (Polymerase Chain Reaction) kit and a gene chip, which are used for detecting aeromonas hydrophila. The specific nucleotide for theaeromonas hydrophila O antigens and the PCR kit and the gene chip, which contain the nucleotide, have high practicability; the PCR kit has the advantages of simple and convenient preparation method, short detection period, rapid speed and high operability; the industrial production is facilitated and the detection cost is relatively lower; the accuracy is high and the sensitivity is high.

The invention relates to a specific nucleotide for aeromonas hydrophila O antigens and application thereof. The nucleotide comprises: (1) at least one of nucleotides shown as SEQ ID NO:1 to SEQ ID NO:8; (2) at least one of nucleotides which are complementary with the nucleotides shown as SEQ ID NO:1 to SEQ ID NO:8. The nucleotides can be used for preparing a PCR (Polymerase Chain Reaction) kit and a gene chip, which are used for detecting aeromonas hydrophila. The specific nucleotide for theaeromonas hydrophila O antigens and the PCR kit and the gene chip, which contain the nucleotide, have high practicability; the PCR kit has the advantages of simple and convenient preparation method, short detection period, rapid speed and high operability; the industrial production is facilitated and the detection cost is relatively lower; the accuracy is high and the sensitivity is high.

The invention relates to a nucleotide specific to Aeromonas hydrophila O antigens and an application thereof. The nucleotide comprises: (1) at least one of nucleotides shown as SEQ ID NO:1 to 8; and (2) at least one of nucleotides being complementary with the nucleotides shown as SEQ ID NO:1 to 8. The nucleotides can be used for preparing a PCR (Polymerase Chain Reaction) kit for detecting Aeromonas hydrophila and a gene chip. The nucleotide specific to the Aeromonas hydrophila O antigens and the PCR kit and the gene chip including the nucleotide have high practicability. The PCR kit has the advantages of easiness and convenience in preparation, short detection period, high speed, high operability, easiness for industrial production, relatively low detection cost, high accuracy and high sensitivity.

For O antigens of Providencia O19, O20, O28, O30, O31 and O36 serotypes, a real-time fluorescent TaqMan polymerase chain reaction rapid detection system based on an MGB probe is used for analysis. According to the invention, the specific gene of the O antigen gene cluster of the Providencia is used as a target gene; a primer and an MGB probe for O antigen typing of the Providencia are designed andscreened, a corresponding real-time fluorescent PCR (RT-PCR) detection method is established, and a credible way is provided for O antigen typing of the aeromonas hydrophila by utilizing a molecularbiological means. The MGB probe disclosed by the invention is used for detecting the prowedenia in excrement, urethral infection, wounds, burns and bacteremia specimens, and O antigen typing is carried out on the Providencia, so that the method has the advantages of high sensitivity, high specificity, rapid detection and the like.

The invention relates to a nucleotide specific to vibrio cholerae O antigens and application of the nucleotide, wherein the nucleotide comprises 1) at least one of nucleotides as shown in SEQ ID NO: 1-8; and 2) at least one of nucleotides supplementary with the nucleotides as shown in SEQ ID NO: 1-8. The nucleotides can be used for preparing a PCR kit and a gene chip for detecting vibrio cholerae. The nucleotide specific to vibrio cholerae O antigens disclosed by the invention as well as the PCR kit and the gene chip containing the nucleotide is strong in practicability; the PCR kit is simple and convenient in preparation method, short in detection cycle, rapid in speed, strong in operability and easy in industrial production, while the PCR kit is relatively low in detection cost; and the nucleotide is high in accuracy and high in sensitivity.

The invention relates to a nucleotide specific to Aeromonas hydrophila O antigens and an application thereof. The nucleotide comprises: (1) at least one of nucleotides shown as SEQ ID NO:1 to 8; and (2) at least one of nucleotides being complementary with the nucleotides shown as SEQ ID NO:1 to 8. The nucleotides can be used for preparing a PCR (Polymerase Chain Reaction) kit for detecting Aeromonas hydrophila and a gene chip. The nucleotide specific to the Aeromonas hydrophila O antigens and the PCR kit and the gene chip including the nucleotide have high practicability. The PCR kit has the advantages of easiness and convenience in preparation, short detection period, high speed, high operability, easiness for industrial production, relatively low detection cost, high accuracy and high sensitivity.