38 results about "O-Antigens" patented technology

Compositions and methods are described for vaccinating against E. coli intra-abdominal infections. The compositions contain a FimH polypeptide, one or more conjugates containing E. coli O-antigens polysaccharide covalently coupled to a carrier protein, and an adjuvant.

The invention provides a nucleotide specific for Escherichia coli 054 O-antigen, which is the total nucleotide sequence of the gene cluster for controlling the synthesis of O-antigens in Escherichia 054, e.g. isolated nucleotide represented by SEQ ID No:1, with overall length of 14062 bases, or nucleotide of SEQ ID No:1 including one or more inserted, deleted or substituted bases and sustains the isolated nucleotide functions, it also includes the oligonucleotides of wzx gines in the O-antigen gene cluster originating from the Escherichia 054, and genes and wzy genes having similar functions with wzx, and genes having similar functions with wzy genes or wzy genes. The invention proves the high specifity of oligonucleotides for Escherichia 054 O-antigen through PCR. A method for detecting and identifying Escherichia 054 in human body and the environment by means of the oligonucleotide according to the invention is also disclosed.

The invention provides a nucleotide specific for shigella bodyii 10 O-antigen, which is the total nucleotide sequence of the gene cluster for controlling the synthesis of O-antigens in Escherichia, e.g. isolated nucleotide represented by SEQ ID No:1, with overall length of 15253 bases, or nucleotide of SEQ ID No:1 including one or more inserted, deleted or substituted bases and sustaining the functions of the isolated nucleotides, it also includes the oligonucleotides of glycosyl transferase genes and oligosaccharide unit treatment genes in the O-antigen gene cluster originated from Shigella dysenteriae 10. The invention proves the high specifity of oligonucleotides for Shigella dysenteriae 10 O-antigen through PCR. A method for detecting and identifying Shigella dysenteriae 10 by means of the oligonucleotide according to the invention is also disclosed.

The present invention relates to method of testing a sample for the presence of nucleic acids encoding E. coli polysaccharide O-antigen serotype O111, S. enterica polysaccharide O-antigen serotype C2 and S. enterica polysaccharide O-antigen serotype B.

Compositions and methods are described for vaccinating against E. coli urinary tract infections. The compositions contain a FimH polypeptide, one or more conjugates containing E. coli O-antigens polysaccharide covalently coupled to a carrier protein, and an adjuvant.

The invention provides a nucleotide specific for Escherichia coli 0145 O-antigen, which is the total nucleotide sequence of the gene cluster for controlling the synthesis of O-antigens in Escherichia, e.g. isolated nucleotide represented by SEQ ID No:1, with overall length of 16932 bases, or nucleotide of SEQ ID No:1 including one or more inserted, deleted or substituted bases and sustaining the functions of the isolated nucleotides, it also includes the oligonucleotides of glycosyl transferase genes and oligosaccharide unit treatment genes in the O-antigen gene cluster originated from Escherichia 0145. The invention proves the high specifity of oligonucleotides for Escherichia coli 0145 O-antigen through PCR. A method for detecting and identifying Escherichia 0145 by means of the oligonucleotide according to the invention is also disclosed.

The invention provides a nucleotide specific for shigella bodyii 10 O-antigen, which is the total nucleotide sequence of the gene cluster for controlling the synthesis of O-antigens in shigella bodyii 10, e.g. isolated nucleotide represented by SEQ ID No:1, with overall length of 13402 bases, or nucleotide of SEQ ID No:1 including one or more inserted, deleted or substituted bases and sustains the isolated nucleotide functions, it also includes the oligonucleotides of wzx gines in the O-antigen gene cluster originating from the shigella bodyii 10, and genes and wzy genes having similar functions with wzx, and genes having similar functions with wzy genes or wzy genes or orf 8 genes with unknown functions. The invention proves the high specifity of oligonucleotides for shigella bodyii 10 O-antigen through PCR. A method for detecting and identifying shigella bodyii 10 in human body and the environment by means of the oligonucleotide according to the invention is also disclosed.

The invention provides a nucleotide specific for Escherichia coli 0141 O-antigen, which is the total nucleotide sequence of the gene cluster for controlling the synthesis of O-antigens in Escherichia 0141, e.g. isolated nucleotide represented by SEQ ID No:1, with overall length of 15601 bases, or nucleotide of SEQ ID No:1 including one or more inserted, deleted or substituted bases and sustains the isolated nucleotide functions, it also includes the oligonucleotides of glycosyl transferase genes and oligosaccharide unit treatment genes in the O-antigen gene cluster originated from Escherichia coli 0141. The invention proves the high specifity of oligonucleotides for Escherichia coli 0141 O-antigen through PCR. A method for detecting and identifying Escherichia coli 0141 in human body and the environment by means of the oligonucleotide according to the invention is also disclosed.

The invention relates to a liquid chip detection method for serotypes of four main pathogenic O antigens of yersinia enterocolon and an application thereof. According to the invention, an O3 type wbbUgene, an O5 type wzt gene, an O8 type wzy gene and an O9 type per gene of yersinia enterocolon are used as target genes, specific primers and probes for each serotype are designed and screened, and acorresponding liquid chip detection method is established. By utilizing the method provided by the invention, molecular biological detection can be accurately, quickly and sensitively carried out onfour main pathogenic O antigen serotype strains of yersinia enterocolon.

The invention provides a nucleotide specific to O-antigens of Escherichia coli O140, a full sequence of gene cluster in Escherichia coli O140, controlling O-antigen synthesis, such as the separated nucleotide shown in SEQ IN No.1, with a overall length of 14009 basic groups; or a nucleotide in SEQ IN No.1 having one or many inserted, deleted or substituted basic groups and simultaneously maintaining functions of the separated nucleotide; also including an oligonucleotide coming from glycosyltransferase gene and oligosaccharide unit-processing gene in the O-antigen gene cluster of Escherichia coli O140; by PCR, the invention verifies that oligonucleotide has specificity to all the O-antigens of Escherichia coli O140; the invention also discloses a method of using the oligonucleotide to detect and identify Escherichia coli O140 in the human body and environment.

The invention provides a nucleotide specific to O-antigens of Escherichia coli O154, a full sequence of gene cluster in Escherichia coli O154, controlling O-antigen synthesis, such as the separated nucleotide shown in SEQ IN No.1, with a overall length of 13635 basic groups; or a nucleotide in SEQ IN No.1 having one or many inserted, deleted or substituted basic groups and simultaneously maintaining functions of the separated nucleotide; also including an oligonucleotide coming from glycosyltransferase gene and oligosaccharide unit-processing gene in the O-antigen gene cluster of Escherichia coli O154; by PCR, the invention verifies that oligonucleotide has specificity to all the O-antigens of Escherichia coli O154; the invention also discloses a method of using the oligonucleotide to detect and identify Escherichia coli O154 in the human body and environment.

The invention provides a nucleotide specific for Escherichia coli 0151 O-antigen, which is the total nucleotide sequence of the gene cluster for controlling the synthesis of O-antigens in Escherichia 0151, e.g. isolated nucleotide represented by SEQ ID No:1, with overall length of 15670 bases, or nucleotide of SEQ ID No:1 including one or more inserted, deleted or substituted bases and sustains the isolated nucleotide functions, it also includes the oligonucleotides of glycosyl transferase genes and oligosaccharide unit treatment genes in the O-antigen gene cluster originated from Escherichia coli 0151. The invention proves the high specifity of oligonucleotides for Escherichia 0151 O-antigen through PCR. A method for detecting and identifying Escherichia coli 0151 in human body and the environment by means of the oligonucleotide according to the invention is also disclosed.

The invention provides a nucleotide specific for Escherichia coli 0156 O-antigen, which is the total nucleotide sequence of the gene cluster for controlling the synthesis of O-antigens in Escherichia coli 0156, e.g. isolated nucleotide represented by SEQ ID No:1, with overall length of 15390 bases, or nucleotide of SEQ ID No:1 including one or more inserted, deleted or substituted bases and sustains the isolated nucleotide functions, it also includes the oligonucleotides of glycosyl transferase genes and oligosaccharide unit treatment genes in the O-antigen gene cluster originated from Escherichia coli 0156. The invention proves the high specifity of oligonucleotides for Escherichia coli 0156 O-antigen through PCR. A method for detecting and identifying Escherichia coli 0156 in human body and the environment by means of the oligonucleotide according to the invention is also disclosed.

For O antigens of Providencia O19, O20, O28, O30, O31 and O36 serotypes, a real-time fluorescent TaqMan polymerase chain reaction rapid detection system based on an MGB probe is used for analysis. According to the invention, the specific gene of the O antigen gene cluster of the Providencia is used as a target gene; a primer and an MGB probe for O antigen typing of the Providencia are designed andscreened, a corresponding real-time fluorescent PCR (RT-PCR) detection method is established, and a credible way is provided for O antigen typing of the aeromonas hydrophila by utilizing a molecularbiological means. The MGB probe disclosed by the invention is used for detecting the prowedenia in excrement, urethral infection, wounds, burns and bacteremia specimens, and O antigen typing is carried out on the Providencia, so that the method has the advantages of high sensitivity, high specificity, rapid detection and the like.

The invention relates to a nucleotide specific to vibrio cholerae O antigens and application of the nucleotide, wherein the nucleotide comprises 1) at least one of nucleotides as shown in SEQ ID NO: 1-8; and 2) at least one of nucleotides supplementary with the nucleotides as shown in SEQ ID NO: 1-8. The nucleotides can be used for preparing a PCR kit and a gene chip for detecting vibrio cholerae. The nucleotide specific to vibrio cholerae O antigens disclosed by the invention as well as the PCR kit and the gene chip containing the nucleotide is strong in practicability; the PCR kit is simple and convenient in preparation method, short in detection cycle, rapid in speed, strong in operability and easy in industrial production, while the PCR kit is relatively low in detection cost; and the nucleotide is high in accuracy and high in sensitivity.