The invention relates to a sterilization and disinfection liquid for clinical laboratory medical staff. The sterilization and disinfection liquid is prepared from, by weight, 10-30 parts of leaves of ligustrum sinense Lour., 5-15 parts of herba inulae, 3-10 parts of calyxes or calyxes with fruit of franchet groundcherry, 10-30 parts of semen sojae germinatum, 10-20 parts of stems or leaves of Amur ampelopsis, 5-15 parts of frangrant ainsliaea herb, 10-30 parts of caulis sargentodoxae, 10-15 parts of lawn pennywort herb, 10-20 parts of shouliang yam tubers, 5-15 parts of herba eupatorii, 20-30 parts of stems and leaves of great burdock, 20-30 parts of herba pyrolae, 5-15 parts of herb of blin conyza, 3-8 parts of all-grass of mosla chinensis Maxim. and 5-10 parts of cortex myricae. The sterilization and disinfection liquid is prepared from the pure natural raw materials, free of stimulus to skin of human bodies, safe, effective and capable of effectively killing staphylococcus aureus, escherichia coli, pneumococcus, streptococcus, pseudomonas aeruginosa and shigella dysenteriae and particularly aiming at the staphylococcus aureus, the pseudomonas aeruginosa and the escherichia coli.