129 results about "Shigella dysenteriae" patented technology

Provided are compositions for attracting and / or stimulating oviposition. The compositions comprise a suitable carrier and a bacterium capable of producing nonanoic acid, tetradecanoic acid, or methyl tetradecanoate; Bacillus thuringiensis; Lactococcus lactis; Klebsiella oxytoca; Shigella dysenteriae; Brevundimonas vesicularis; a supernatant of a culture of any of the these bacteria; nonanoic acid; tetradecanoic acid; or methyl tetradecanoate, or any combination thereof, at a concentration effective to attract the mosquito to a target. The compositions may be comprised within a kit or trap. Also provided are methods of attracting mosquitoes to a target.

The invention relates to laundry detergent, in particular to efficient laundry detergent with bacterial inhibition and sterilization functions. The laundry detergent comprises components in percentage by weight as follows: 17%-23% of a surfactant, 1%-3% of an acid aid, 0.2%-0.5% of caustic soda flakes, 0.3%-0.5% of a chelating agent, 3%-6% of heartleaf houttuynia herb extract, 0.2%-0.4% of a preservative, 1%-2% of a thickening agent, 0.5%-1% of acorus gramineus essential oil, 1%-2% of fructus amomi essential oil, 1%-2% of licorice extract, 0.6%-1.5% of tea saponin, 0.4%-0.8% of lavender essential oil, 0.6%-1.2% of mint essential oil and the balance of deionized water. The efficient laundry detergent has an inhibiting function on streptococcus hemolyticus, staphylococcus aureus, hemophilus influenza, catarrhalis, pneumococcus, escherichia coli, shigella dysenteriae and salmonella typhi, also has an inhibiting function on various viruses and further has a body immunity enhancing function and an anti-tumor effect.

The invention relates to a Chinese herbal medicinal wet wipe for cleaning and disinfection of the skin, which relates to the technical field of daily necessities. The wet wipe comprises a wet wipe solution and a carrier for the wet wipe solution. The invention is characterized in that raw materials for preparation of the wet wipe solution comprise, by weight, 0.5 to 1% of lavender essential oil, 0.4 to 0.8% of cassia twig essential oil, 0.3 to 1% of cablin patchouli herb essential oil, 0.01 to 0.1% of menthol, 0.01 to 0.2% of paeonol, 0.3 to 1% of a coptis extract, 0.2 to 1% of a witch hazel extract, 0.5 to 2% of hydrogenated castor oil and 5 to 10 % of ethanol, with the balance being water. According to the invention, a non-woven fabric is used as the carrier; the wet wipe is convenient to use and can kill common pathogenic bacteria like escherichia coli, staphylococcus aureus, bacillus subtilis, aspergillus flavus, Gram-positive cocci, shigella dysenteriae, helicobacter pylori and Candida albicans; the wet wipe is mild-natured, natural and safe because of utilization of a Chinese herbal medicine formula and can overcome the problems of irritation and corrosivity of currently-used chemical disinfection products.

The invention relates to a multiplex PCR-based synchronous and rapid method for detecting 13 pathogenic microorganisms in water, which comprises the steps of using multiplex PCR to simultaneously amplify gene-specific fragments of the 13 pathogenic microorganisms including escherichia coli, enterohaemorragic escherichia coli o157:h7, legionella pneumophila, salmonella enteritidis, shigella dysenteriae, staphyloccocus aureus, listeria monoeytogenes, helicobacter pylori, mycobacterium tuberculosis, klebsiella pneumonia, vibrio cholera, bacillus anthracis and yersinia pestis, and detecting PCR amplified products through agarose gel electrophoresis, thereby achieving synchronous and rapid detection for the 13 pathogenic microorganisms.

The invention discloses a primer for detection of shigella and a detection method. The sequence of the primer for the detection of the shigella is expressed as SEQ ID NO: 1-4. The detection method for the shigella comprises the following steps of: (1) extracting DNA serving as a template; (2) adopting a loop-mediated isothermal amplification reaction system which comprises 2muL of template, 2muL of primer, 1muL of Bst DNA and 7.5 to 12.5muL of amplification reaction solution, adding water into the reaction system to obtain 25muL of reaction system, performing thermostatic reaction for 1 to 1.5 hours at the temperature of between 63 and 65 DEG C, then placing the reaction system at the temperature of 80 DEG C for 2 to 10 minutes, and terminating the reaction; and (3) observing the results, namely observing a detection tube by using naked eyes, wherein the result is positive shigella when turbid is produced, and the result is negative when no turbid is produced. The method for detecting the shigella by using the primer to perform the loop-mediated isothermal amplification reaction does not need special equipment, can quickly detect shigella dysenteriae genes in a detection sample, and has the advantages of specificity, high sensitivity and simple identification method.

The invention provides an oral composition which is an oral preparation prepared by taking Periplaneta americana extract and Punicosides as active ingredients, wherein the weight ratio of the Periplaneta americana extract to Punicosides is 1:1-1:5. The invention also provides a preparation method and application of the oral composition. According to the oral composition disclosed by the invention, toothpaste can perform synchronous nursing on the oral cavity, teeth and gingiva and can effectively solve dental ulcer, the Punicosides have high in-vivo anti-oxidation and free radical cleaning activities, and the oral composition has a broad-spectrum antibacterial effect, has obvious effects of inhibiting staphylococcus aureus, hemolytic streptococcus, comma bacillus and shigella dysenteriae and has the effects of astringing, stopping bleeding, improving eyesight and the like.

The invention discloses a compound andrographolidume preparation for veterinary use. The compound andrographolidume preparation comprises the following components in parts by weight: 10-100 parts of andrographolidume, 5-50 parts of sophocarpidine, 0-20 parts of a stabilizer, 0-985 parts of a solvent and 0-985 parts of auxiliary materials. The compound preparation prepared from two traditional Chinese medicine effective ingredients of the andrographolidume and the sophocarpidine as main drugs has better broad-spectrum antibacterial and anti-viral effects and has different inhibition effects on shigella dysenteriae, escherichia coli, staphylococcus aureus, diplococcus pneumoniae, streptococcocci, various viruses and the like; the sophocarpidine is an alkaloid effective ingredient extracted from sophors flavescens ait and has a remarkable inhibition effect on shigella dysenteriae, escherichia coli, salmonella, proteusbacillus vulgaris, staphylococcus aureus, streptococcocci and amebic protozoa; the clinical treatment effect of the compound preparation on intestinal diseases of livestock is remarkable and superior to that of a prescribed preparation of a prescription medication of the compound preparation.

The invention is based on the discovery of the epitope in the Stx1 protein for the 13C4 antibody. The invention features non-full length Stx1 polypeptides that include the epitope for the 13C4 monoclonal antibody epitope. The invention also features methods of producing anti-Stx1 antibodies specific for the 13C4 epitope of the Stx1 protein. Additionally, the invention features methods for treating a subject having, or at risk of developing, a Shiga toxin associated disease (e.g., hemolytic uremia syndrome and diseases associated with E. coli and S. dysenteriae infection) with a polypeptide that includes the 13C4 epitope or with an anti-Stx1 antibody developed using the methods of the invention. Furthermore, the invention features the detection of Stx1 in a sample using the antibodies developed using the methods of the invention.

The present invention discloses a grass carp enteritis treatment drug composition, which contains, by weight, 10-30 parts of humulus scandens, 20-30 parts of humifuse euphorbia herb, 2-10 parts of melia azedarach leaf, 20-25 parts of heartleaf houttuynia herb, 2-10 parts of arborvitae leaf, and 2-7 parts of garlic. The invention further discloses a preparation method for the drug composition. According to the grass carp enteritis treatment drug composition, honeysuckle provides an anti-pathogenic microorganism effect, a certain inhibition effect is provided for a variety of pathogenic bacteria such as staphylococcus aureus, hemolytic streptococcus, escherichia coli, shigella dysenteriae, vibrio cholerae, typhoid bacillus, paratyphoid bacillus and the like, and a certain inhibition effect is further provided for pneumococcus, meningococcus, pseudomonas aeruginosa, and tubercle bacillus. The preparation method is simple and easy to perform, and is suitable for mass production.

The invention discloses an environment-friendly biological pesticide which is characterized by comprising the following components in parts by weight: 0.001-0.002 parts of shigella dysenteriae bacillus aceticus powder, 0.001-0.002 parts of bdellovibrio powder, 0.001-0.002 parts of phage powder, 0.001-0.002 parts of oceanospirillales powder, 20-26 parts of tea seed cake, 10-16 parts of rape seed cake, 1-10 parts of synergist, 5-20 parts of taraxacum, 1-3 parts of rhizoma smilacis glabrae, 1-3 parts of ground beeltle, 1-3 parts of indian adenosma, 1-3 parts of pericarpium arecae, 1-3 parts of screwtree roots, 12-18 parts of houttuynia cordata, 5-18 parts of common andrographis herb and 100-150 parts of water. The environment-friendly pesticide is a novel pesticide which is efficient, targeted, environment-friendly and nuisanceless, has no secondary toxicity, residue or pollution, and keeps the ecological balance, is an important guarantee for achieving ecological agriculture, green ecological agriculture and a green enterprise, and has a wide prospect.

The invention discloses a Shigella flora / serotype multiplex-PCR (polymerase chain reaction) detection primer set and kit. The detection primer set is composed of primer pairs for detecting Shigella sonnei, type-1-5 Shigella flexneri, type-I Shigella dysenteriae, type-6 Shigella flexneri, and Shigella. According to the invention, a multiplex-PCR detection method based on a common PCR platform is built; according to the method, multiplex-PCR reaction is carried out on total DNA of bacteria extracted from a sample to be detected in a same reaction system by using the primer pairs obtained through analysis and design, and through electrophoretic analysis on a reaction product, whether the sample is Shigella can be determined and Shigella flora / serotype can be judged.

The invention relates to a gene chip for detecting enterohemorrhagic escherichia coli, comprising a solid-phase carrier and an oligonucleotide specific probe fixed on the solid-phase carrier, wherein the oligonucleotide specific probe fixed on the solid-phase carrier has at least one of the following gene sequences: at least one DNA fragment selected from oligosaccharide unit treatment enzyme gene sequences in O-antigen gene clusters of different sero-group escherichia coli; a DNA fragment selected from coding genes of enterohemorrhagic escherichia coli enterotoxin; a DNA fragment selected from shigella dysenteriae type-1 genes; and a complementary DNA or RNA fragment of the previous DNA fragments. The invention also relates to a use method of the gene chip and a kit comprising the gene chip. The gene chip and the kit can achieve the purpose of detecting the enterohemorrhagic escherichia coli and have simple and convenient operation, high accuracy and strong repeatability.

The invention relates to the aplication of amigo-smile polyoses used for resisting bacteria or viruses, in particular to the application of the amigo-smile polyoses for preparing a medicament with the effects of resisting the bacteria or the viruses. The amigo-smile polyoses of the invention has inhibition effects to a plurality of bacteria or viruses, such as Bacillus subtilis, Staphylococcus aureus, Micrococcus luteus, Escherichia coli, salmonella, Proteus vuigaris, Shigella dysenteriae, Candida albicans or parainfluenza virus, and the like. The known method of the prior art can be adopted to prepare the amigo-smile polyoses of the invention into a medicament compound and the medicament compounds of any preparation formulation acceptable on pharmacy, such as granular formulation, oral liquid, capsule, troche, injection, pulvis, and the like.

The invention belongs to a rapid detection technology of food-borne pathogens, in particular to a loop-mediated isothermal amplification (LAMP) method for detecting shigella dysenteriae DNA. The method comprises the preparation of reagent comprising sample processing reagent, LAMP reactive reagent, a large fragment of Bst DNA polyase, agarose, bromophenol blue loading buffer solution and ethidium bromide solution and the procedures of the detection of amplification products and sediment generation. The method comprises the following steps: firstly, designing a specific primer; secondly, extracting sample DNA; and thirdly, preparing a reaction system. Compared with the currently adopted traditional bacterial culture method, an immunological detection method and other molecular biology methods, the method has the advantages of simple and rapid operation, no special instrument and reagent, sensitivity and specificity and can be applied to the rapid detection of shigella dysenteriae in foods and other samples.

The invention relates to a pollution-free hog house disinfectant prepared from the following raw materials in parts by weight: 12-15 parts of rhizoma anemarrhenae, 5-7 parts of catalpa leaf, 20-30 parts of rheum officinale, 5-12 parts of pericarpium citri reticulatae viride, 12-15 parts of cortex magnoliae officinalis, 20-30 parts of agastache rugosus, 10-15 parts of elsholtzia ciliata, 3-5 parts of cyrtomium rhizome, 6-9 parts of common andrographis herb, 3-7 parts of negundo chastetree fruit, 15-20 parts of table salt, and 5-12 parts of sodium bicarbonate. The product has quite good bactericidal and bacteriostatic effects, can effectively inhibit and kill pathogenic bacteria, fungi and viruses in the environment, also has inhibition and killing effects on various parasites, and especially has quite good bacteriostatic effect on escherichia coli, staphylococcus aureus and shigella dysenteriae; and the product is natural, safe and pollution-free, can be used in various stages of hog breeding, meets demands of consumers on pollution-free breeding and requirements of modern hog industry, and has broad market prospects.

The invention relates to the field of microecological feed additives, and particularly relates to preparation and application of a composite microecological feed additive. The composite microecological feed additive provided by the invention is prepared from screened active compound probiotics. Diarrhea caused by domestic animal bacterial infections can be prevented and treated. Two probiotics used by the composite microecological feed additive have the abilities of inhibiting infection of common gastrointestinal pathogens of animals, including shigella dysenteriae, pathogenic escherichia coli and salmonella enteritidis, and also can reduce the toxicity caused by dysentery bacillus infection Caco-2 cells and inhibit adhesion of the shigella dysenteriae to the Caco-2 cells. Finally, the obvious effects of stimulating the animal immunity, improving the gastrointestinal digestion abilities of animals, and promoting animal growth are achieved. A production technology of a strain adopts a liquid fermental cultivation technology and a low-temperature freeze drying technology, so that the obtained inoculant is high in survival rate and purity. The composite microecological feed additive is a green environment-friendly feed additive.

A nucleotide specific to the O-antigen of Shigella dysenteriae 3, Escherichia coli O124 and Escherichia coil O164 is a complete nucleotide sequence of the genom for controlling the synthesis of O-antigen is shigella dysenteriae 3, such as the separated nucleotide shown by SEQ ID No.1. It has 12629 bases, or has one or more inserted, deletional or substituted bases but keeps the function of said separated neucleotide. It also includes the oligonucleotide of glycosyltransferase gene and oligose unit treating gene in O-antigen genom. A method for using said oligonucleotide to detect said three bacteria is also disclosed.

The invention discloses a henhouse disinfectant formula which consists of the following raw materials in parts by weight: 5-10% of an onion original liquid, 5-10% of a sanguisorba officinalis extract liquid, 0.5-1% of hexachlorophene, 1-3% of formalin with the formaldehyde content of 40%, 0.5-1% of sodium dichloroisocyanurate, and 0.01%-0.02% of an additive named 'Chuqin'an' according to the Chinese pronunciation; a henhouse disinfectant is prepared by mixing and stirring evenly the raw materials according to the proportion. The onion original liquid and the sanguisorba officinalis extract liquid are used as the main raw materials of the disinfectant, the onion original liquid has the effect of killing staphylococcus aureus, corynebacterium diphtheriae and the like, the sanguisorba officinalis extract liquid can kill various strains of typhoid fever or paratyphoid fever pathogens and shigella dysenteriae in one minute, and the two components cooperate with a chemical disinfectant, so that the disinfectant cannot make pathogenic bacteria generate the drug resistance and has strong disinfection effect and the like.

The invention relates to a sterilization and disinfection liquid for clinical laboratory medical staff. The sterilization and disinfection liquid is prepared from, by weight, 10-30 parts of leaves of ligustrum sinense Lour., 5-15 parts of herba inulae, 3-10 parts of calyxes or calyxes with fruit of franchet groundcherry, 10-30 parts of semen sojae germinatum, 10-20 parts of stems or leaves of Amur ampelopsis, 5-15 parts of frangrant ainsliaea herb, 10-30 parts of caulis sargentodoxae, 10-15 parts of lawn pennywort herb, 10-20 parts of shouliang yam tubers, 5-15 parts of herba eupatorii, 20-30 parts of stems and leaves of great burdock, 20-30 parts of herba pyrolae, 5-15 parts of herb of blin conyza, 3-8 parts of all-grass of mosla chinensis Maxim. and 5-10 parts of cortex myricae. The sterilization and disinfection liquid is prepared from the pure natural raw materials, free of stimulus to skin of human bodies, safe, effective and capable of effectively killing staphylococcus aureus, escherichia coli, pneumococcus, streptococcus, pseudomonas aeruginosa and shigella dysenteriae and particularly aiming at the staphylococcus aureus, the pseudomonas aeruginosa and the escherichia coli.

A method for comparing the variable reactivity of multiple, differentially mutated copies of 16S subsequences found in a number of ribosomal RNA operons of a single bacterial cell is described. The application of this method for distinguishing between closely related organisms, such as the genera Escherichia and Shigella, and between species of Shigella including S. boydii, S. dysenteriae, S. flexneri, and S. sonnei using nucleic acid probes is also presented.

The invention discloses a method for preparing a natural red jujube skin pigment and application of the natural red jujube skin pigment. The method comprises the following steps of: removing jujube flesh from red jujubes to obtain red jujube skin; drying the red jujube skin, crushing, leaching in a weak base solution, and filtering to obtain a red jujube skin pigment stock solution; and concentrating, drying and crushing the red jujube skin pigment stock solution to obtain powdered natural red jujube skin pigment. According to the method, a process is simple and low in cost and is suitable for large-scale production; and the powdered red jujube skin pigment ensures the stability and wide application of the pigment. The prepared natural red jujube skin pigment has the antibacterial activity on gram-positive bacteria of staphylococcus aureus, listeria monocytogenes, lactobacillus, alicyclobacillus and the like and on gram negative bacteria of escherichia coli, proteus, shigella dysenteriae, pneumobacillus, acinetobacter and the like.

The invention discloses a fish feed additive capable of clearing intestine and stopping diarrhea, and a preparation method thereof. According to the preparation method, animal waste is changed into biogas residue with utility value via biogas anaerobic fermentation; and the fish feed additive is prepared via further processing so as to bring benefits to cultivation and protect the environment. According to the preparation method, the biogas residue obtained via biogas fermentation is subjected to mixing granulation and particle spraying, so that nutritional ingredients are enriched, unpleasant odor is changed, palatability is improved, and feeding attraction is improved. According to the preparation method, particles obtained via granulation are subjected to grease spraying, so that floatability of the particles in water is increased, it is convenient for ingestion of fish, dissolving of the particles in water is slowed, and utilization ratio is increased. According to the preparation method, traditional Chinese medicine raw materials such as the root of Chinese pulsatilla, cortex fraxini, cortex phellodendri, rhizoma coptidis, and rhizoma atractylodis macrocephalae are added, so that shigella dysenteriae is inhibited; bacteria and parasites in fish are killed; and the fish feed additive is capable of clearing intestine and stopping diarrhea, and preventing enteritis.

The invention discloses a lipopolysaccharide synthetase gene cluster recombinant escherichia coli capable of expressing Shigella dysenteriae 1 type pathogenic bacteria. The escherichia coli contains rfbR (rhamnosyltransferase), rfbQ (rhamnosyltransferase) and orf9 (galactosyltransferase) in Shigella dysenteriae 1 type pathogenic bacteria rfb gene cluster, and the genotype is W3110 delta wbbl / p-rfp+p-0-Ag. The invention also discloses application of the escherichia coli to preparation of Shigella1 type pathogenic bacterium resistant glycoprotein vaccine. The recombinant escherichia coli can produce lipopolysaccharide structure of Shigella dysenteriae 1 type pathogenic bacteria; the escherichia coli is used as a platform, so that the Shigella1 type pathogenic bacterium resistant glycoprotein vaccine can be further efficiently produced at low cost; good industrial development and application prospects are realized.

The invention relates to Salmonella typhi Ty21a comprising core-linked Shigella dysenteriae serotype 1 O-specific polysaccharide (O-Ps) and DNA encoding O antigen biosynthesis, said DNA selected from the group consisting of: a) the DNA sequence set out in any one of SEQ ID NOs: 1 and 2 and species homologs thereof; b) DNA encoding Shigella dysenteriae serotype 1 polypeptides encoded by any one of SEQ ID NOs: 1 and 2, and species homologs thereof; and c) DNA encoding a O antigen biosynthesis gene product that hybridizes under moderately stringent conditions to the DNA of (a) or (b); and related sequences, compositions of matter, vaccines, methods of using, and methods of making.

The invention discloses a Shigella multivalent conjugate vaccine which includes five serotypes, i.e., Shigella dysenteriae I type, Shigella flexneri 2a type, Shigella flexneri 3a type, Shigella flexneri 6 type and Shigella sonnei; the Shigella multivalent conjugate vaccine is prepared by the steps of preparing conjugate stock solutions from specific polysaccharides of the bacteria and carrier protein, and mixing five conjugate stock solutions in a proper proportion. The prepared conjugate vaccine disclosed by the invention is applicable to immunoprophylaxis of Shigella infection.

The invention provides a nucleotide special to the O-antigen of Shigella dysenteriae 13, namely the nucleotide total sequence of gene group controlling the composition of the O-antigen in Shigella dysenteriae 13. It also includes the O-antigen gene group's construction and the oligonucleotide deriving from the glycosyl-transfer ase gene and the oligose unit processing gene (including wzx gene or the gene with the function similar to wzx gene and wzy gene or the gene with the function similar to wzx gene) in the O-antigen group of Shigella dysenteriae 13, and includes the method to get the O-antigen gene group. It verifies high speciality of the oligonucleotide. It also discloses the method of testing the oligonucleotide and determining Shigella dysenteriae 13 in human body and the environment.

The invention provides a nucleotide specific for shigella bodyii 10 O-antigen, which is the total nucleotide sequence of the gene cluster for controlling the synthesis of O-antigens in Escherichia, e.g. isolated nucleotide represented by SEQ ID No:1, with overall length of 15253 bases, or nucleotide of SEQ ID No:1 including one or more inserted, deleted or substituted bases and sustaining the functions of the isolated nucleotides, it also includes the oligonucleotides of glycosyl transferase genes and oligosaccharide unit treatment genes in the O-antigen gene cluster originated from Shigella dysenteriae 10. The invention proves the high specifity of oligonucleotides for Shigella dysenteriae 10 O-antigen through PCR. A method for detecting and identifying Shigella dysenteriae 10 by means of the oligonucleotide according to the invention is also disclosed.

The invention relates to a Chinese medicinal decoction for treating white scour of piglets and a preparation method thereof. A preparation method comprises the following steps of: picking red-knees herb plants, removing silt and cleaning, airing, cutting into filaments of 0.5 centimeter, putting the filaments into water, decocting for 1 to 2 hours, filtering, and extracting liquid medicine to obtain the Chinese medicinal decoction for treating the white scour of piglets. In the Chinese medicinal decoction, red-knees herb is pungent in taste and warm in nature, and has the effects of dispelling wind, removing dampness, detoxicating, relieving swelling, astringing to arrest diarrhea, eliminating stasis and relieving pain; extracts of the red-knees herb has the high bacteriostatic effect on staphylococcus aureus, pseudomonas aeruginosa, hemolytic streptococcus, shigella dysenteriae and escherichia coli; and the Chinese medicinal decoction has an obvious effect on the treatment of the white scour of the piglets and does not have the medicinal residues, and clinical symptoms such as diarrhea of ill domestic animals and the like disappear after administration.