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Use of Eupatorium adenophorum polysaccharide for antibiosis or antivirus

A polysaccharide technology of Eupatorium adenophorum and Eupatorium adenophorum, which is applied in the direction of antibacterial drugs, antifungal agents, and resistance to vector-borne diseases. It can solve the problems of polysaccharides that have not been seen yet, and achieve simple methods, low production costs, and high polysaccharide content in products. high effect

Inactive Publication Date: 2009-05-06
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among the published methods for the development and utilization of Eupatorium adenophorum, there are no reports on the preparation of polysaccharides and their uses.

Method used

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  • Use of Eupatorium adenophorum polysaccharide for antibiosis or antivirus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Take an appropriate amount of crushed whole plant of Eupatorium adenophorum, heat and reflux with 5 times the amount of distilled water, extract 3 times, each extraction time is 3, 2, 1 hour respectively, combine the three extracts, filter with 150-mesh double-layer filter cloth, and the filtrate is in Use thin film evaporation at 70°C to concentrate to 1 / 3 of the original volume; add 5 times 75% ethanol to the concentrated extract under stirring to precipitate the polysaccharide in the concentrated extract, and collect the crude precipitate of Eupatoria adenophorum polysaccharide; Add distilled water to the crude polysaccharide precipitate to dissolve it, make it fully redissolved, use the sevag method to remove the protein, repeat it 9 times, concentrate (the method is the same as above), add 5 times of 75% methanol to the concentrated solution to carry out alcohol precipitation, and use for precipitation Wash with absolute ethanol, acetone and ether respectively to ob...

Embodiment 2

[0040] Take an appropriate amount of ground Eupatorium adenophorum, heat and reflux with 20 times the amount of distilled water, extract 4 times, each extraction time is 3, 2, and 1 hour, combine the three extracts, filter with 100-mesh double-layer filter cloth, and the filtrate is in Use thin film evaporation at 50°C to concentrate to 1 / 5 of the original volume; add 2 times 95% ethanol to the concentrated extract under stirring to precipitate the polysaccharide in the concentrated extract, and collect the crude precipitate of Eupatoria adenophorum polysaccharide; Add distilled water to the crude polysaccharide precipitate to dissolve it, make it fully redissolved, use the sevag method to remove the protein, repeat 3 times, concentrate (the method is the same as above), add 3 times of 85% methanol to the concentrated solution to carry out alcohol precipitation, and use for precipitation Wash with absolute ethanol, acetone and ether respectively to obtain Eupatorium adenophorum...

Embodiment 3

[0044] Take an appropriate amount of crushed whole Eupatoria adenophorum, soak it in 5 times the amount of distilled water for 2 hours, and extract the residue with microwave once under the conditions of microwave power of 600W, solid-liquid weight ratio of 1:5, and microwave extraction time of 2 minutes. Add the same volume of water to the ballast and soak for 1 hour, then microwave twice to extract; combine the two extracts, filter through a 150-mesh double-layer filter cloth, and concentrate the filtrate to 1 / 3 of the original volume under reduced pressure at 70°C; Add 5 times of 75% ethanol to the extract, precipitate the polysaccharide in the concentrated extract, collect the coarse precipitate of Eupatorium adenophorum polysaccharide; add distilled water to the coarse precipitate of polysaccharide to dissolve it, make it fully redissolved, and use the sevag method Deproteinization, repeated 9 times, concentration (method as above), adding 5 times of 75% methanol to the co...

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Abstract

The invention relates to the aplication of amigo-smile polyoses used for resisting bacteria or viruses, in particular to the application of the amigo-smile polyoses for preparing a medicament with the effects of resisting the bacteria or the viruses. The amigo-smile polyoses of the invention has inhibition effects to a plurality of bacteria or viruses, such as Bacillus subtilis, Staphylococcus aureus, Micrococcus luteus, Escherichia coli, salmonella, Proteus vuigaris, Shigella dysenteriae, Candida albicans or parainfluenza virus, and the like. The known method of the prior art can be adopted to prepare the amigo-smile polyoses of the invention into a medicament compound and the medicament compounds of any preparation formulation acceptable on pharmacy, such as granular formulation, oral liquid, capsule, troche, injection, pulvis, and the like.

Description

1. Technical field [0001] The invention relates to an Eupatorium adenophorum polysaccharide, in particular to an antibacterial and antiviral application of the Eupatorium adenophorum polysaccharide extracted from a plant of Eupatorium adenophorum, and belongs to the field of medicinal natural products. 2. Background technology [0002] Eupatorium adenophorum (Eupatorium adenophorum Spreng) is a perennial herbaceous plant of the genus Eupatorium adenophorum, commonly known as airplane grass and devil's grass. Originally produced in Mexico, it entered the southern part of my country through Australia in the 1940s, and is now widely distributed in Yunnan, Hainan, Guangxi, Guizhou, Tibet, Sichuan and other provinces. At the same time, Eupatorium adenophorum contains toxic substances, which often cause horses, cattle, sheep and other grazing livestock to be poisoned or even die by mistake. Eupatorium adenophorum has caused irreparable and huge losses to agriculture, animal husba...

Claims

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Application Information

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IPC IPC(8): A61K31/715A61P31/04A61P31/10
CPCY02A50/30
Inventor 高平苏文涛皮宁宁苏东海
Owner SICHUAN UNIV
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