The invention relates to a dairy cow breast disinfectant, and belongs to the fields of biomedicine and medicaments for agriculture and livestock. The organic dairy cow breast disinfectant is characterized by comprising the following raw materials in percentage by mass according to the formula: 0.25 to 1 percent of 99 percent glycerol, 0.25 to 1 percent of synthetic Chinese medicinal herb concentrate, and the balance of deionized water. The Chinese medicinal herb concentrate is prepared from extracts of honeysuckle, indigowoad leaf, Chinese violet, muskroot-like semiaquilegia root, atractylodes rhizome, folium artemisiae argyi, phyllanthus ruinaria, dandelion, sophora flavescens, glabrous greenbrier rhizome, fructus cnidii, patrinia and groundsel by cleaning, extraction, re-extraction of primary residue, synthesis, decolorization and concentration, and the density of the concentrate is more than or equal to 1.04g/mm<3>. The disinfectant is used for diminishing dairy cow breast inflammation caused by invasion of multiple microbes such as candida albicans, escherichia coli, streptococcus, microsporum, salmonella and shigella and controlling invasion of pathogenic microbes on breasts and mammary glands of dairy cows.