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Jute root extract with antibacterial activity and preparation method thereof

A technology of antibacterial activity and root extract, which is applied in the field of application, can solve the problems that the extraction of antibacterial components and its application research have not been reported, and achieve the effect of high antibacterial activity

Inactive Publication Date: 2011-06-15
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But the antibacterial test research relevant with hemp is relatively few at present, and research object mainly concentrates on the fiber of apocynum hemp, hemp, flax etc.; There are few reports about the bacteriostasis of root, leaf etc. of hemp plant; The extraction and application of antibacterial components of jute plants have not been reported yet

Method used

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  • Jute root extract with antibacterial activity and preparation method thereof

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Effect test

Embodiment 1

[0018] Take jute root as raw material, wash, dry, pass through an 80-mesh sieve after crushing, add 10 times the amount of 95% (v / v) ethanol, extract at 40°C for 120 minutes, and use ultrasonic-assisted extraction. The extraction conditions are: The temperature is 40°C, the ultrasonic power is 200W, and the ultrasonic time is 25min. After extraction, filter and collect the filtrate, concentrate the filtrate to 1 / 2 volume under reduced pressure, and put it on the HPD100 macroporous resin column for dynamic adsorption. The process conditions are: flow rate 5-10ml / min, adsorption time 3h, using 30 % acetone elution, analysis time 3h, acetone recovery, to obtain jute root extract rich in antibacterial active ingredients.

Embodiment 2

[0020] Take jute root as raw material, wash, dry, pass through an 80-mesh sieve after crushing, add 15 times the amount of 95% (v / v) ethanol, extract at 40°C for 120 minutes, and use ultrasonic-assisted extraction. The extraction conditions are: The temperature is 40°C, the ultrasonic power is 200W, and the ultrasonic time is 25min. After extraction, filter and collect the filtrate, concentrate the filtrate to 1 / 3 volume under reduced pressure, and put it on HPD100 macroporous resin column for dynamic adsorption. 30% acetone elution, analysis time 3h, acetone recovery, to obtain jute root extract rich in antibacterial active ingredients.

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Abstract

The invention discloses a jute root extract with antibacterial activity and a preparation method thereof. The method relates to a comprehensive jute utilization technology, and is used for preparing extracts. The jute root extract serves as a natural antibacterial preservative and an antibacterial finishing agent, and belongs to the field of application of foods, medicaments and textiles. The method comprises the following steps of: washing jute root serving as a raw material with water; drying; grinding; extracting ethanol under ultrasonic assistance; concentrating under the reduced pressure; and adsorbing and purifying with a macroporous resin to obtain the jute root extract with high antibacterial activity. The antibacterial effect of the jute root extract is detected by using gram-positive bacterium staphylococcus aureus, bacillus subtilis, candida albicans and gram-negative bacteria escherichia coli as tested bacteria by an in-vitro bacteria inhibition zone method. By adopting the method, the jute root extract with antibacterial activity has best antibacterial effect on the staphylococcus aureus and bacillus subtilis, better antibacterial effect on candida albicans and good antibacterial effect on escherichia coli.

Description

Technical field: [0001] The invention discloses a jute root extract with antibacterial activity and a preparation method thereof, which is a jute comprehensive utilization technology, and is used to prepare the extract as a natural antibacterial preservative and antibacterial finishing agent, and belongs to the application fields of food, medicine, textiles and the like. Background technique: [0002] Jute, an annual herb of the genus Corchorus of the Tiliaceae family, has a long history of cultivation in my country. Due to the high content of lignin and hemicellulose in jute fiber, the fiber is thick and hard, the count is low, the elasticity and elongation at break are also low, so the spinnability is poor, and it has been used to process sacks, sacks and other packaging materials. Jute is rich in yield, low in price, and has better ecological and environmental protection characteristics such as antibacterial, anti-mildew, anti-ultraviolet radiation, and easy degradation. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K47/46A23L3/3472
Inventor 吕兵章军
Owner JIANGNAN UNIV
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