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Application of effective part of cockroach extract in preparing drug for inhibiting fungus growth

A technology of effective parts and extracts, which is applied in the field of preparation of antifungal infection drugs, can solve problems such as poor metabolic properties and physical and chemical properties, poor bioavailability, and inconvenient use for patients, and achieve potential social and economic benefits and increase income , Conducive to the effect of industrialization

Inactive Publication Date: 2013-03-20
DALI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the biggest problem facing echinocandycin is: there is no oral dosage form, only limited to injection (and limited to hospital use), so it is quite inconvenient for patients to use
Although the new product has an expanded antibacterial spectrum, it has poor metabolic properties and physical and chemical properties, low water solubility, and poor bioavailability; patients need a high-fat diet to facilitate drug absorption, or take expensive special preparations to take effect; in order to increase water solubility The added cyclodextrin also poses a greater threat to those with renal insufficiency

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1 : Use D-101 macroporous adsorption resin to enrich the lyophilized powder raw material of effective parts of cockroaches

[0024] 1.1 Preparation of the alcoholic extract of Periplaneta americana: Add 3 kg of Periplaneta americana medicinal materials, add 10,000 ml of 55% ethanol, soak and extract at room temperature, extract 3 times, 24 hours each time. The extracts were combined, concentrated to dryness under reduced pressure, and dried in vacuum to obtain the alcoholic extract of Periplaneta americana.

[0025] 1.2 Preparation of different extracts from D-101 macroporous adsorption resin column chromatography: 20 grams of alcoholic extract of Periplaneta americana, mixed with 30 grams of D-101 macroporous adsorption resin, and 200 grams of D-101 macroporous Adsorption resin column, first eluted with water until colorless, then eluted with 30% ethanol-water solution until colorless, took 10 ml of the eluent and concentrated it to dryness under reduced press...

Embodiment 2

[0027] Example 2 : Detection of antifungal activity of effective parts of Periplaneta americana alcohol extract D-101 macroporous adsorption resin column chromatography 60% alcohol lotion

[0028] Referring to the standardized antifungal susceptibility test method proposed by the National Committee for Clinical Laboratory Standards (NCCLS), the in vitro antifungal activity of the effective fraction I-60 prepared in Example 1 was tested.

[0029] 2.1 Fungal standard strain:

[0030] Candida albicans ATCC10231: Provided by Chongqing Center for Disease Control;

[0031] Candida albicans ATCC90029 (5-FC drug-resistant strain): Provided by the Clinical Laboratory Center of the Ministry of Health of Beijing Hospital;

[0032] Candida albicans ATCC76615 (CS3): Provided by Changzheng Hospital Affiliated to Second Military Medical University.

[0033] Candida albicans 98001: provided by Wuhan Culture Collection Center;

[0034] Candida albicans ATCC76615:19 (Y0119): Provided by Na...

Embodiment 3

[0077] Example 3 : Acute toxicity (LD 50 )test

[0078] 3.1 Experimental materials:

[0079] Sample to be tested: the effective fraction I-60 prepared according to Example 1;

[0080] Solvent: 0.5%CMC-Na;

[0081] Animals: ICR mice, both male and female, weighing 19.0-20.9 g, were purchased from Shanghai Slack Experimental Animal Co., Ltd.

[0082] 3.2 Experimental method:

[0083] 3.2.1 Preparation of sample suspension: Weigh the effective fraction I-60 sample to prepare a suspension with a concentration of 50 mg / ml.

[0084] 3.2.2 Administration: 12 mice (half male and half female) were administered intragastrically with I-60 suspension of the effective part; the administration volume was 40 ml / kg, and the administration dose was 2000 mg / kg.

[0085] 3.2.3 Observation record: observe and record the reaction of the mice after administration.

[0086] 3.3 Experimental results: The feeding and activity of the animals were observed continuously for 10 days after administ...

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Abstract

The invention relates to application of an effective part of a cockroach extract in preparing a drug for inhibiting fungus growth, and particularly relates to application of an effective part obtained by refining a crude cockroach extract through macroporous adsorption resin in the aspect of preparing a drug capable of resisting fungal infection. The effective part is refined from a fresh or dried Periplaneta americana L. polypide product through alcohol and water extraction, macroporous adsorption resin column chromatography and alcohol solvent elution, and has an obvious inhibition effect on the growth activity of candida albicans and a fungus growth inhibition capacity 80 times higher than that of fluconazole and 5-fluorocytosine and 800 times higher than that of amphotericin. The effective part of the cockroach extract can be prepared into forms, such as a hydrogel, a frothy gel, a cataplasm, freeze-dried powder, a water aqua, an aerosol, a suppository, a film, an external liniment, an ointment and the like, and is used for preparing pharmaceutical preparations for preventing and curing fungal infection, medical appliances and daily-use chemical products.

Description

technical field [0001] The present invention relates to the technical field of medicine, in particular, the present invention relates to the application of the effective part obtained by refining the crude extract of cockroaches through macroporous adsorption resin material column chromatography in the preparation of antifungal infection drugs. Background technique [0002] With the development of medical science, various new drugs and new technologies such as broad-spectrum antibiotics, hormones, various catheter intubation techniques, chemotherapy, transplantation and other medical methods are widely used, as well as HIV infection and tumors, causing the human immune system to be gradually The number of cases of destruction is increasing year by year, which leads to an increasing incidence of deep mycoses; on the other hand, with the widespread use of antibiotics and clinical antifungal drugs, traditional antibiotics cannot effectively fight against some serious fungal infe...

Claims

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Application Information

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IPC IPC(8): A61K35/64A01N61/00A61P31/10A01P3/00
Inventor 白丽高鹏陈俊雅周萍高鹏飞赵昱钱金栿
Owner DALI UNIV
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