The invention discloses powder for strengthening body resistance and eliminating tumor, and a preparation method and application thereof. The powder is prepared from red ginseng, caterpillar fungus, human placenta, scorpion, centipede, trogopterus dung, long-nosed pit viper, antlers sheet, pangolin scales, ground beetle, notoginseng root, dogtooth violet, dragon's blood, amber, myrrh, dried ginger, asafetida, gecko, zedoary, fructus amomi, muskroot-like semiaquilegia root, airpotato yam rhizome, fritillaria, hive, vietnamese sophora root, cicada shell, eleutherococcus senticosus, raw largehead atractylodes rhizome, solanum nigrum and hairyvein agrimonia herb and bud. The powder is convenient to prepare, can be used for treating various cyst, fibroma, myoma, malignant and the like effectively, and has a good therapeutic effect and an auxiliary effect for treating diseases such as abdominal distention, poor appetite, weakness and pain caused by various malignant tumors.