6 results about "Nandina" patented technology

The invention relates to the technical field of soil improvement and soil pollution treatment, in particular to a method for repairing waste coal mine area soil through nandina domestica. According to the method, soil remediation is carried out on the abandoned coal mine area by combining chemical and biological improvement methods, compost, wood charcoal and ferric sulfate are adopted as a composite soil remediation agent, and nandina domestica is planted, so that the soil acidity and the heavy metal flowability of the abandoned coal mine area are reduced, and the soil environment of the abandoned coal mine area is further improved.

The application discloses a three-dimensional cell scaffold based on a leaf vein of nandina domestica and a preparation method and application thereof, relates to the technical field of biomedical materials and tissue engineering, and discloses the following steps: taking the leaf vein of nandina domestica as a base material, and preparing the scaffold through alkali boiling, sodium periodate selective oxidation and amino-silica / chitosan composite modification. The scaffold retains the three-dimensional structure of the natural leaf vein completely, and significantly improves the cell compatibility of the material. Cell experiments prove that the scaffold has no cytotoxicity, can support the three-dimensional growth of mesenchymal stem cells and PC12 cells and the like with high survival rate, and effectively promotes cell proliferation. The method is simple, green, environmentally-friendly and low in cost, and the obtained scaffold has wide application prospects in the fields of tissue engineering, regenerative medicine and drug screening and the like.

The invention discloses a method for extracting berberine from nandina domestica and a berberine extract prepared by the method. The method comprises the following steps: carrying out compound enzyme enzymolysis treatment on a nandina raw material, carrying out ventilated extraction on the nandina raw material and NADES at 40-55 DEG C for 40-60 minutes, and filtering an extracting solution to remove solid impurities; and separating and purifying the filtered leaching solution, and further purifying the berberine through methods such as crystallization or recrystallization to obtain a high-purity berberine extract product. According to the method, the content and yield of berberine in the product can be remarkably improved, the content of berberine in the product can reach 98.6%, and the yield of berberine in the product can reach 85.22%.

The utility model discloses a cuttage pool device for ruby nandina breeding, and belongs to the technical field of ruby nandina breeding, the cuttage pool device for ruby nandina breeding comprises a seedbed, an arc-shaped frame is installed above the seedbed, and a shunt pipe is arranged below the arc-shaped frame; five groups of four-way atomizers are arranged below a shunt pipe, in actual use, a cuttage pool is arranged in a greenhouse, automatic intermittent spraying formed by an electromagnetic valve, a time automatic controller and electronic leaves can provide the most suitable rooting condition for cuttage of twigs with leaves, the four-way atomizers can spray cutting slips through intermittent spraying, and the cutting slips can be sprayed by the four-way atomizers. According to the method, a water film is kept on the surface of the cutting slips, so that the cutting slips are not withered due to water loss before rooting, the temperature of the cutting slips can be reduced due to transpiration of water on the surface of the cutting slips, the cutting slips are not burnt even under the burning sun in summer, and on the contrary, the cutting slips are subjected to sufficient photosynthesis, and rapid rooting and survival of the cutting slips are promoted.

The invention provides a construction method for determining the HPLC (High Performance Liquid Chromatography) content of nandina domestica, which comprises the following steps: A) dissolving a test raw material by adopting a solvent, and extracting to obtain a to-be-detected solution; b) determining the to-be-detected liquid by adopting a high performance liquid chromatography to obtain an HPLC spectrum of the nandina domestica; chromatographic conditions of the high performance liquid chromatography are as follows: a chromatographic column is a C18 column; a mobile phase A is an acetonitrile solution, a mobile phase B is a 0.1% phosphoric acid aqueous solution, and gradient elution is carried out; the gradient elution is specifically as follows: 0-20 min, the phase A is 10%-11%, and the phase B is 90%-89%; 20-25 min, the phase A accounts for 11%-18%, and the phase B accounts for 89%-82%; 25-40 min, the phase A accounts for 18%-40%, and the phase B accounts for 82%-60%. According to the present invention, the high performance liquid chromatography is adopted, the acetonitrile-0. 1% phosphoric acid solution is adopted as the mobile phase to perform gradient elution, and the chlorogenic acid reference substance, the neochlorogenic acid reference substance and the cryptochlorogenic acid reference substance are adopted as the reference substances to establish the HPLC content determination method of the nandina domestica so as to provide more scientific technical means for controlling the medicinal quality of the nandina domestica.

The utility model belongs to the technical field of seedling raising boxes, and particularly relates to a shrub seedling raising box which comprises a box body. A fluorescent lamp is fixedly installed at the top in the box body, a water tank is fixedly installed at the bottom in the box body, supports are fixedly installed on the two sides of the top of the water tank, an installation plate is slidably arranged at the upper ends of the supports, through holes are formed in the upper portions of the two ends of the installation plate, and the installation plate is slidably installed on the supports through the through holes. Sliding rails are fixedly mounted at the bottom of the mounting plate, sliding grooves are formed in one sides of the sliding rails, a seedling raising plate is arranged between the sliding rails, the two sides of the seedling raising plate are slidably mounted in the sliding grooves of the sliding rails, a plurality of seedling raising grooves are formed in the seedling raising plate in an array mode, water passing holes are formed in the bottoms of seedling raising grass, and a water tank is arranged below the seedling raising plate. The bottom of the water tank is fixedly mounted at the lower end of the bracket through a fixing block; the seedling raising plate is immersed into the bottom of the water tank, nandina domestica in the seedling raising plate is irrigated, the irrigation process is gentle, and leaves and stems are not prone to being damaged.