2 results about "Nandina" patented technology

The application discloses a three-dimensional cell scaffold based on a leaf vein of nandina domestica and a preparation method and application thereof, relates to the technical field of biomedical materials and tissue engineering, and discloses the following steps: taking the leaf vein of nandina domestica as a base material, and preparing the scaffold through alkali boiling, sodium periodate selective oxidation and amino-silica / chitosan composite modification. The scaffold retains the three-dimensional structure of the natural leaf vein completely, and significantly improves the cell compatibility of the material. Cell experiments prove that the scaffold has no cytotoxicity, can support the three-dimensional growth of mesenchymal stem cells and PC12 cells and the like with high survival rate, and effectively promotes cell proliferation. The method is simple, green, environmentally-friendly and low in cost, and the obtained scaffold has wide application prospects in the fields of tissue engineering, regenerative medicine and drug screening and the like.

The invention discloses a method for extracting berberine from nandina domestica and a berberine extract prepared by the method. The method comprises the following steps: carrying out compound enzyme enzymolysis treatment on a nandina raw material, carrying out ventilated extraction on the nandina raw material and NADES at 40-55 DEG C for 40-60 minutes, and filtering an extracting solution to remove solid impurities; and separating and purifying the filtered leaching solution, and further purifying the berberine through methods such as crystallization or recrystallization to obtain a high-purity berberine extract product. According to the method, the content and yield of berberine in the product can be remarkably improved, the content of berberine in the product can reach 98.6%, and the yield of berberine in the product can reach 85.22%.