The invention discloses an application preparation applied to an eyelid to relieve visual fatigue and a preparation method thereof, and belongs to the field of medicine. The application preparation ismainly prepared from semen celosiae, fructus ligustri lucidi, semen cassiae, pearl, calcined calamine, rhizoma acori graminei, herba menthae, storax, chrysanthemum flower, folium mori, borneol, ethylparaben, cod-liver oil, animal bile and purified water. The application preparation can relieve relevant symptoms by being directly applied to the eyelid for 10 minutes, is safe and convenient, and has a remarkable curative effect. The safe, convenient and effective application preparation for relieving visual fatigue can relieve visual fatigue rapidly, raise the eye hygiene level and prevent myopia, is also suitable for people suffering from cataract, glaucoma, vitreous opacity, macular degeneration, retinal detachment, dacryocystitis and the like, and has the effects of relieving eye discomfort and refreshing brain and eyes.