Method for preparing degradable artificial ductulus lacrimalis

A lacrimal canaliculus, artificial technology, applied in the direction of catheters, eye implants, etc., to achieve the effect of simple operation, not easy to break or deform, and avoid re-damage

Active Publication Date: 2008-05-07
SHANGHAI QISHENG BIOLOGICAL PREPARATION CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

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Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] A preparation method of degradable artificial tear canaliculus is:

[0049] Step 1. Dissolve medical polyvinyl alcohol PVA-124 particles in water for injection in a water bath at 90°C to prepare a transparent 5-10% (w / w) polyvinyl alcohol solution;

[0050] The second step. At room temperature, dissolve chitosan with a degree of deacetylation ≥90% in 1-2% (w / w) acetic acid solution and stir for 16-24 hours until it is completely dissolved. ~4%(w / w) chitosan solution, refrigerated at 2℃~8℃;

[0051] The third step. Pepsin digestion, acetic acid solution extraction and cleaned bovine Achilles tendon, from which the type I collagen solution is extracted, the concentration is ≥0.5% (w / w), and it is refrigerated at 2℃~8℃;

[0052] Step 4. Weigh 90g 1% collagen solution and 10g 2% chitosan solution, stir and mix in an ice bath, add 50g 10% polyvinyl alcohol solution, stir for about 1 hour and mix until the mixed solution becomes uniform and translucent Viscous liquid

[0053] Ste...

Embodiment 2

[0064] Weigh 70g of 1% collagen solution and 30g of 2% chitosan solution, stir and mix well in an ice bath. Add 50g 10% polyvinyl alcohol solution, continue to stir and mix until the mixed solution forms a uniform translucent viscous liquid, slowly add a little 2M NaOH solution dropwise while stirring, adjust the pH of the solution to 7.08, continue to stir for half an hour, low temperature Let stand for one night. Use a syringe to draw up the homogeneous mixed solution, put a needle on, inject the solution into the mold, and then repeatedly freeze / thaw at -20℃ / 20℃ for 5 times, take out the columnar elastic composite gel, and insert a hard composite gel in the middle of the composite gel column. Make a hole with the quality round rod, and then put it in water for injection for 30 minutes, stand it upright, air dry at room temperature, demold, trim both ends, and encapsulate. The rest is the same as in Example 1.

Embodiment 3

[0066] Weigh 30 g of 1% collagen solution and 20 g of 2% chitosan solution, stir and mix in an ice bath, add 25 g of 10% polyvinyl alcohol solution, continue to stir and mix until the mixed solution forms a uniform translucent viscous liquid. While stirring, slowly add a little 2M NaOH solution dropwise to adjust the pH of the solution to 6.89, continue stirring for half an hour, and let it stand overnight at low temperature. Use a syringe to draw the mixed solution, put on a needle, inject the solution into the mold, and then repeatedly freeze / thaw at -20°C / 20°C for 5 times, take out the columnar composite gel, and pass the 7# puncture needle through the gel column Center shaft, soak the needled gel column in ≤0.25% glutaraldehyde solution for 0.5 hours. After taking it out, rinse it repeatedly in water for injection, stand it up, air dry at room temperature, demould, cut both ends, encapsulate, and the rest with Example 1 is the same.

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Abstract

The invention discloses a preparation method of a degradable artificial lacrimal canaliculus and its clinical application in treating lacrimal duct obstruction or dacryocystitis. The main components of the artificial lacrimal canaliculus are biodegradable type I collagen, chitosan and medical polyvinyl alcohol. Type I collagen has the effect of rapidly stopping bleeding and promoting wound healing, chitosan can play the role of antibacterial, anti-adhesion, and anti-scar, and polyvinyl alcohol enhances the supporting capacity of artificial lacrimal canaliculus. The lacrimal canaliculus produced by the invention has an inner diameter of 0.5-0.7mm, an outer diameter of 0.9-1.5mm, and a length of ≥20mm. The ingredients are evenly mixed, the inner and outer surfaces are smooth, and the lacrimal canaliculus has good toughness and elasticity, and is convenient for clinical operations. After 10 to 30 minutes of contact with water, it quickly absorbs water and swells, which can play a role in mechanical positioning and preventing the lacrimal canaliculus from slipping. After the wound is healed, the small tube is gradually degraded, and the degradation product can flow out from the nasal cavity with tears, without the need for secondary surgery to remove it, which improves the effect of treating lacrimal duct obstruction and avoids abrasions caused by secondary surgery. According to the different requirements of degradation time, a certain degree of chemical cross-linking can be carried out on the lacrimal canaliculus to adjust its degradation rate in the lacrimal duct.

Description

Technical field [0001] The invention relates to a method for preparing a degradable artificial lacrimal canaliculus, in particular to a method for rapidly preparing a degradable artificial lacrimal canaliculus by forming a gel column, which is clinically used to treat lacrimal duct obstruction and dacryocystitis. It belongs to the technical field of artificial tear canaliculus in ophthalmology. Background technique [0002] In ophthalmology treatment, there are more and more cases of lacrimal duct obstruction and acute / chronic dacryocystitis caused by various causes, accounting for about 3% of outpatient ophthalmology cases, and tears or bleeding caused by obstruction of the lacrimal duct Bring great inconvenience to patients' life and work. At present, the commonly used method to treat obstruction of the lacrimal duct is mechanical or laser probing, followed by repeated washing with liquid medicine. However, the difficulty that is difficult to solve is that after the first probi...

Claims

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Application Information

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IPC IPC(8): A61L29/14A61L29/12A61L29/04A61F2/14
Inventor 郑贵球蒋丽霞顾其胜
Owner SHANGHAI QISHENG BIOLOGICAL PREPARATION CO LTD
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