Method for preparing degradable artificial ductulus lacrimalis

A canaliculus, artificial technology, applied in the direction of catheters, eye implants, etc., to achieve the effect of simple operation, uniform distribution, and easy demoulding

Active Publication Date: 2011-07-27
SHANGHAI QISHENG BIOLOGICAL PREPARATION CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no relevant report on the preparation of collagen, chitosan and polyvinyl alcohol into artificial lacrimal canaliculi

Method used

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  • Method for preparing degradable artificial ductulus lacrimalis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] A preparation method of degradable artificial lacrimal canaliculus is as follows:

[0049] Step 1. Dissolve the medical polyvinyl alcohol PVA-124 particles in water for injection in a water bath at 90°C to prepare a transparent 5-10% (w / w) polyvinyl alcohol solution;

[0050] The second step. Under room temperature conditions, the chitosan with deacetylation degree ≥ 90% is dissolved in 1 ~ 2% (w / w) acetic acid solution, stirred for 16 hours-24 hours, until fully dissolved, prepared into 2 ~4% (w / w) chitosan solution, refrigerated at 2°C to 8°C;

[0051] Step 3. Pepsin digestion, acetic acid solution extraction and washing of bovine Achilles tendon, from which extract type I collagen solution, concentration ≥ 0.5% (w / w), refrigerated at 2°C to 8°C;

[0052] The fourth step. Weigh 90g 1% collagen solution and 10g 2% chitosan solution, stir and mix in ice bath, add 50g 10% polyvinyl alcohol solution, stir for 1 hour and mix until the mixed solution forms a uniform transl...

Embodiment 2

[0064] Weigh 70g of 1% collagen solution and 30g of 2% chitosan solution, stir and mix in ice bath. Add 50g of 10% polyvinyl alcohol solution, continue to stir and mix until the mixed solution forms a uniform translucent viscous liquid, slowly add a little 2M NaOH solution dropwise while stirring, adjust the pH value of the solution to 7.08, continue to stir for half an hour, Let stand overnight. Use a syringe to draw a homogeneous mixed solution, put a needle on it, inject the solution into the mold, and then freeze / thaw repeatedly at -20°C / 20°C for 5 times, take out the elastic composite gel formed into a column, and insert a hard gel into the middle of the composite gel column. Make a hole with a round rod, put it into water for injection and wash it for 30 minutes, stand it upright, air-dry it at room temperature, remove the mold, trim both ends, and package, and the rest are the same as in Example 1.

Embodiment 3

[0066] Weigh 30g of 1% collagen solution and 20g of 2% chitosan solution, stir and mix in an ice bath, add 25g of 10% polyvinyl alcohol solution, continue to stir and mix until the mixed solution forms a uniform translucent viscous liquid. Slowly add a little 2M NaOH solution dropwise while stirring to adjust the pH value of the solution to 6.89, continue stirring for half an hour, and let stand overnight at low temperature. Use a syringe to draw the mixed solution, put a needle on it, inject the solution into the mold, then freeze / thaw 5 times at -20°C / 20°C, take out the columnar composite gel, and pass the 7# puncture needle through the gel column For the central axis, soak the needle-piercing gel column in ≤0.25% glutaraldehyde solution for 0.5 hours, take it out, rinse it repeatedly in water for injection, stand it upright, dry it naturally at room temperature, demould, cut both ends, encapsulate, and the rest with Example 1 is the same.

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Abstract

The invention discloses a preparation method of a degradable artificial lacrimal duct, and the clinical application of the lacrimal duct on the treatment of lacrimal passage blockage or dacryocystitis. The main components of the artificial lacrimal duct comprises biodegradable I type collagen, chitosan, and medical polyvinyl alcohol. The I type collagen has the effect of rapidly arresting bleeding and accelerating the wound healing, the chitosan has the function of bacteriostasis, anti blocking and anti cicatrix, and the polyvinyl alcohol enhances the supporting ability of the artificial lacrimal duct. The inner diameter of the invention is 0.5 to 0.7 mm, the outer diameter is 0.9 to 1.5 mm, for the lacrimal duct which has length larger than or equal to 20 mm, the components mixing is even, the inner surface and the outer surface are flat, thereby having good tenacity and elasticity, and being suitable for the clinical operation. After the lacrimal duct is contacted with water for 10 to 30 minutes, the water is absorbed rapidly and the invention is swelled, and the functions of mechanical locating and preventing the lacrimal duct from gliding can be realized. After the wound is healed, the lacrimal duct is gradually degraded, the degradation product can flow out from the nasal cavity along with tears, and the second time operation is not required to take off the degradation product, the effect of curing the blockage of the lacrimal passage is improved, and the abrasion caused by the second time operation is avoided. Chemical crosslink in a specific degree also can be performed to the lacrimal duct according to different requirements of degrading time to adjust the degrading speed inside the lacrimal passage.

Description

technical field [0001] The present invention relates to a preparation method of a degradable artificial lacrimal canaliculus, in particular to a method of rapidly making a degradable artificial lacrimal canaliculus by forming a gel column, which is used clinically to treat lacrimal duct obstruction and dacryocystitis. The invention belongs to the technical field of artificial lacrimal canaliculus in ophthalmology. Background technique [0002] In ophthalmic treatment, there are more and more cases of lacrimal duct obstruction and acute / chronic dacryocystitis caused by various etiologies, accounting for about 3% of outpatient cases in ophthalmology. Bring great inconvenience to patient's life and work. At present, the commonly used method for treating lacrimal duct obstruction is mechanical or laser probing, followed by repeated washing with water. However, the difficulty that is difficult to solve is that after the first probing, tissue re-adhesion easily occurs at the ori...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L29/14A61L29/12A61L29/04A61F2/14
Inventor 郑贵球蒋丽霞顾其胜
Owner SHANGHAI QISHENG BIOLOGICAL PREPARATION CO LTD
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