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Semiaquilegia root extract, preparation method and application thereof

A technology of extract, Tiankui, applied in the preparation of medicine for preventing and treating cataract, in the field of Tiankui extract, achieving the effect of suppressing turbidity, preventing and treating cataract, and low cost

Inactive Publication Date: 2010-07-14
汤磊
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the research and related application of Tiankui for the prevention and treatment of cataract have not been reported yet.

Method used

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  • Semiaquilegia root extract, preparation method and application thereof
  • Semiaquilegia root extract, preparation method and application thereof
  • Semiaquilegia root extract, preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Embodiment 1 of the present invention: preparation of geranium extract sample

[0031] Take the fresh or dried geranium, chop it up, and extract with water, methanol, ethanol, acetone; or a mixed solvent of water, methanol, ethanol, or acetone in any proportion as the extraction solvent. The extraction method is decoction, cold immersion, reflux, ultrasonic extraction, supercritical fluid extraction or microwave extraction, and the obtained extract is evaporated under reduced pressure, vacuum freeze-dried or spray-dried to remove the solvent to obtain the geranium extract. The specific raw materials and their weight, the extraction solvent used, the extraction method adopted, and the solvent removal method are shown in Table 1.

[0032] The preparation method of table 1 geranium extract sample

[0033]

[0034]

Embodiment 2

[0035]Example 2 of the present invention: identification of characteristic active ingredients in geranium extract

[0036] 1. TLC identification of tangelofolidine

[0037] Weigh about 1 g of the samples TK1-TK13 in Example 1 and place them in a 20 mL Erlenmeyer flask with a stopper, add 20.00 mL of methanol, ultrasonically extract for 30 min, take out, filter, and concentrate the filtrate in a water bath to 5 mL, respectively as the test samples TK1-TK6 solution. In addition, take the reference substance of tangconidine, add methanol to make a solution with a concentration of 1mg / mL, and use it as the reference substance solution. According to "Chinese Pharmacopoeia" 2005 edition an appendix VIB thin-layer chromatography, draw need testing solution 10 μ L respectively, reference substance solution 2 μ L, point on the same silica gel GF254 thin-layer plate, with n-butanol-glacial acetic acid-water (7 μ L) : 1: 2) solution is the developing agent, second development, take out...

Embodiment 3

[0041] Example 3 of the present invention: further purification of geranium extract samples

[0042] 1. Take an appropriate amount of geranium extract (TK5), disperse and dissolve it with an appropriate amount of water, repeatedly extract it several times with petroleum ether, then repeatedly extract the water layer with ethyl acetate and n-butanol, and recover the solvent respectively to obtain the ethyl acetate part ( TK5A) and n-butanol fraction (TK5B) and water fraction (TK5C).

[0043] 2. Take an appropriate amount of geranium extract (TK1), dissolve it with a small amount of methanol, put it on the D-101 macroporous resin column that has been pretreated, first elute with distilled water, then elute with 20% ethanol, and then use Elute with 50% ethanol, collect the 50% ethanol eluate, and recover the solvent to obtain a further purified sample (TK1A).

[0044] 3. Take an appropriate amount of geranium extract (TK1), dissolve it with a small amount of methanol and put it ...

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Abstract

The invention discloses a semiaquilegia root extract, which contains meadow rue cresidine, Griffonillide and lithospermoside. And in addition, the invention also discloses a preparation method of semiaquilegia root extract, which comprises the following steps of: taking fresh or dried semiaquilegia root, cutting, extracting with solvent extraction, and then removing the solvent of the obtained solvent extraction to get semiaquilegia root extract. The invention also discloses application of the semiaquilegia root extract on the preparation of medicine for preventing and treating cataract. The preparation and purification process of the semiaquilegia root extract and purified semiaquilegia root extract is simple and easy, and has obvious effect when being used as medicine for preventing and treating cataract. The highlight improvement and obvious effect of the invention are that the semiaquilegia root is Chinese medicinal herb which is common and easy to take in southwest of China, East China, northeast of China and the like, has low cost and is natural medicine for preventing and treating cataract.

Description

technical field [0001] The invention relates to a natural herbal medicine extract, in particular to a geranium extract, a preparation method and an application of the geranium extract in preparing cataract prevention and treatment medicines. The geranium in the present invention specifically refers to the whole herb whose scientific name is Semiaguilegia adoxoides (DC.) Makino. Background technique [0002] Cataract is an eye disease in which the lens becomes cloudy due to various factors as a result of light scattering due to the lens clouding, so that visual disturbance occurs locally because the light scattering causes inability to form a normal image on the retina. [0003] According to its etiology, cataracts can be divided into the following categories: congenital cataracts, senile cataracts, complicated cataracts, traumatic cataracts, diabetic cataracts, and others. In addition, there are known radioactive cataracts. and glass worker's cataract, etc., all of which co...

Claims

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Application Information

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IPC IPC(8): A61K36/71A61P27/12
Inventor 汤磊郭宗华肖海涛侯富才王建塔
Owner 汤磊
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