The invention provides a preparation method of hot pot red oil, which has a recipe consisting of vegetable oil, star anise, galangal, long pepper, lithospermum, citronella, spiceleaf, kaempferiae, elecampane, pericarpium zanthoxyli, citron, amomun kravanh, dried orange peel, angelica dahurica, xin dogwood flowers, camphortree root, grass shell ginkgo, cinnamon, fructus amomi globosi, Shuizhizi, ageratum, cumin, common fennel, liquorice, pepper, rhizoma zingiberis, amomum villosum, angelica, longan, allspice, muscade, clove, ginkgo, wild pepper, purple cardamomum and meat cardamomum. The hot pot red oil of the invention has unique taste, eaters have rich mouth feelings, the hot pot red oil has strong fragrance and long aftertaste, and in addition, the hot pot red oil has good health care effect. The manufacture method is simple, the cost is low, the hot pot red oil can be instantly eaten after being made, and sterilization can also be carried out after the hot pot red oil are bagged. The hot pot red oil of the invention can be used for seasoning various hot pots, and can be prepared into different flavors as required to meet the requirements of different regions and different eating habits.