46results about How to "Simplify the cultivation procedure" patented technology

The invention relates to a method for expressing an antibacterial peptide apidaecin by using Escherichia coli and for preparing the antibacterial peptide apidaecin. The method comprises the following steps: cloning AP2 gene and a polyhedron gene sequence polh into a recombinant vector in order to form a recombinant vector with a polh-AP fusion gene fragment; cloning the polh-AP fusion gene fragment into an expression vector, converting the expression vector to an expression host cell, and culturing the expression host cell to express a fusion protein with an antibacterial peptide AP2; and culturing the expression host cell to induce the expression of the recombinant protein polh-AP, centrifuging induced bacterial strains, collecting, re-suspending, carrying out ultrasonic fragmentation, centrifuging, collecting the above obtained insoluble inclusion body, purifying, and collecting a recombinant protein sample. An expression system has the characteristics of simple expression system culture program, low production cost, efficient expression of the antibacterial peptide apidaecin, and realization of AP2 and polh fusion expression, so compared with the prior art, the method has the advantages of effective reduction of toxicity of the AP2 to Escherichia coli, improvement of the stability of the antibacterial peptide, realization of high level expression of the antibacterial peptide, convenient purification of the active antibacterial peptide, and improvement of the output of the antibacterial peptide apidaecin.

The invention relates to a method for promoting germination through release of pyrus betulaefolia seed dormancy by sodium malonate and belongs to the technical field of plant production. The method for promoting the germination of pyrus betulaefolia seeds comprises the following main steps: 1) collection and cleaning of seeds; 2) seed soaking in clean water and seed disinfection; 3) treatment by a chemical agent and seed cleaning; and 4) sowing. The pyrus betulaefolia seed treated by the sodium malonate is applied, begins to germinate in three days after the sowing and can totally germinate in one week around; and the germination rate is high and can reach more than 85 percent. The method initially finds that the pyrus betulaefolia seed can release the seed dormancy through the treatment of the sodium malonate without low-temperature lamination treatment. The application of the research can effectively shorten the germinating time and cross-breeding cycle of pyrus betulaefolia and simplify the embryo culturing procedure.

The invention relates to a method for cultivating Rhodiola rosea aseptic seedlings with well-developed root system, and belongs to the field of modern biological methods. The method comprises the following steps: (1), collecting wild Rhodiola rosea seedlings; (2), disinfecting the collected stems of Rhodiola rosea wild seedlings; (3), collecting young rhodiola rosea alpine stems (4) preparation of culture medium; (5) cultivation of aseptic seedlings of Rhodiola rosea with well-developed root system. The present invention overcomes the disadvantages and deficiencies such as weak seedling growth, difficult rooting, less root number, and short seedling age in the existing Rhodiola rosea tissue culture process, and provides a method for cultivating sterile seedlings of Rhodiola rosea with well-developed root system, In order to obtain high-quality regenerated plants of Rhodiola rosea.

The invention discloses a culture medium for culturing antrodia camphorata by vessel culturing and a preparation method thereof. The culture medium is prepared from the following raw materials in parts by weight: 200 to 300 parts of potato leaching powder, 20 to 40 parts of glucose, 0 to 20 parts of soluble soybean meal, 1 to 20 parts of malt extractant, 0.5 to 1.5 parts of magnesium sulfate, 10 to 60 parts of a mixture of a cinnamomum plant, 0.5 to 1.5 parts of potassium dihydrogen phosphate, 0.01 to 0.05 part of vitamin B1, 0 to 0.1 part of chloramphenicol, 15 to 25 parts of agar and 1,000 parts of ultrapure water. According to the culture medium disclosed by the invention, a culturing vessel is used for culturing the antrodia camphorata; the culturing condition has strong controllability; the culturing procedure is simplified; the sundry bacteria contamination can be reduced; the heavy metal pollution is avoided too; the growth increment of antrodia camphorata mycelium or sporocarp is increased as well; and the production cost is lowered.

The invention discloses a method and application for inducing direct somatic embryogenesis. According to the method and application, function genes RID3(AT3G49180) and YUC10(AT1G48910) capable of promoting direct somatic embryogenesis are found. Through ectopic expression of the function genes, inducing can be directly conducted on arabidopsis cotyledons to form the somatic embryo without any callus. The method for inducing direct somatic embryogenesis has the advantages of being simple in culture, short in inducing period, high in inducing frequency and the like, and can be used for study onaspects of differentiation, development and totipotency expression of botanical cells, crop variety improvement, production of artificial seeds, storage of high-quality germplasm resources, mutant screening and the like.

The invention provides a tissue cultivation method of vetiveria zizanioides transplants. The method comprises steps that: sterilizated vetiveria zizanioides explants are placed in induction and proliferation culture media; inducting and proliferation cultivating are carried out concurrently until explants are differentiated and young buds are germinated; times of subcultures are carried out untila required number of seedlings are obtained; the seedlings are transplanted into cultivation media to be cultivated until 3 to 5 roots appear; the seedlings are then transplanted into cultivation bags; routine managements of watering and fertilizing are carried out, and vetiveria zizanioides transplants can be obtained after 40 days of growth. With the method provided by the present invention, usage amounts of drugs can be reduced. Through the concurrent inducting and proliferation cultivating, appearances of variants can be efficiently controlled, tissue cultivating processes can be simplified, propagation periods can be shortened, production cost can be reduced, and merits of vetiveria zizanioides varieties can be kept fast and stably. With the method provided by the present invention, problems in methods with common propagation systems, such as complex parent resources, unstable seedling qualities and slow seedling propagations, can be solved.

The invention discloses a one-step plantlet formation medium and method for the isolated culture of lamiophlomis rotata. By adopting the one-step plantlet formation medium, a lamiophlomis rotata test-tube plantlet leaf forms a plantlet in one step through isolated culture. The one-step plantlet formation culture medium is characterized in that a MS (Murashige and Skoog) medium is used as a basic medium, and 6-benzylaminopurine (6-BA), naphthylacetic acid (NAA) and 2,4-dichlorphenoxyacetic acid (2,4-D) which are different in concentrations and combinations are added to the base medium. According to the one-step plantlet formation method, the test-tube plantlet leaf serving as an explant is inoculated, then is induced by a callus and is directly differentiated into an adventitious bud and an adventitious root, so that the lamiophlomis rotata regenerated plantlet is obtained by one step. By adopting the one-step plantlet formation method, the callus obtained by induction in an initial medium does not need to be transferred into a differential medium for regenerating the plantlet. Compared with an isolated culture multi-step regeneration plantlet formation method, the one-step plantlet formation method has the advantages that the steps are simplified, the culture period is short, the repeatability is good, and the planting percent is high.

The invention discloses a one-step seedling rapid propagation method for tissue culture of Chinese cabbage, which belongs to the technical field of plant tissue culture. The invention solves the problems of low coefficient, slow growth and difficulty in large-scale production in conventional propagation methods of the cabbage. The method is carried out according to the following steps: Step 1, disinfection and sterilization; Step 2, one-step seedling cultivation; Step 3, domestication and transplanting of test-tube seedlings: take out the rooted seedlings and put them in a seedling hardening room with sufficient light After 3 to 5 days, open the bottle mouth and continue to harden the seedlings for 2 to 3 days; then take out the test tube seedlings, wash the root culture medium, and transplant them into the substrate. The substrate is composed of peat soil and vermiculite, and the weight of peat soil and vermiculite The ratio of parts is 1:1, and the seedlings are sprayed 2-3 times a day for the first 7 days. The invention is used for tissue culture of Chinese cabbage.

A provided dendrobium aphyllum tissue-culture quick propagation method comprises: germination of cracked mature seeds obtained by inbred of dendrobium aphyllum, forming and differentiation of protocorms, rooting culture, culture of complete plants, transplanting and surviving. Compared with conventional propagation methods such as high-position bud cutting or strain segregating, the method provided by the invention employs tissue culture technology for propagation of dendrobium aphyllum, and the tissue culture of dendrobium aphyllum is performed by employing protocorms, so that the propagation speed is fast, a large number of seedlings are obtained and grow well, and thus a large number of dendrobium aphyllum seedlings with same properties are cultured in a short period, the transplanting survival rate is high, the market demand is satisfied, and the method has various advantages such as low cost, short period, high yield and the like.

The invention discloses a medium for cultivating a wild paxillus involutus stock culture. The medium is prepared from, by mass, 15-25g of soluble starch, 2-6g of peptone, 1-5g of KH2PO4, 0.5-4.5g of MgSO4, 1L of water and 2-6 of pH. The medium has the advantages of early germination, rapid growth, good growth, high mycelial biomass, short production cycle and the like, the biological characteristics of the bacterium are mastered, and a foundation is laid for further development of an application value and artificial domestication and cultivation. The successful cultivation of the stock culturealso provides new germplasm resources for the development and utilization of medicinal ingredients and teaching and researching, and has great economic and social benefits.

The invention discloses a tissue culture breeding method for acorus gramineus. The tissue culture breeding method comprises the steps of explant disinfection, induction, enrichment culture, subculture, rooting culture, seedling hardening and transplanting. According to the method, the acorus gramineus is high in reproduction speed, and roots and seedlings are robust; the cost and the production time are greatly saved; the problems that the source of parents of seedlings produced by a conventional breeding system is complicated and the quality of the seedlings is unstable; the tissue culture breeding method is suitable for standard, industrial and large-scale production; high-quality seedlings of the uniform standard are provided for the market; the problem that acorus gramineus seedlings cannot be yearly produced is solved.