46results about How to "Simplify the cultivation procedure" patented technology

The invention relates to a method for expressing an antibacterial peptide apidaecin by using Escherichia coli and for preparing the antibacterial peptide apidaecin. The method comprises the following steps: cloning AP2 gene and a polyhedron gene sequence polh into a recombinant vector in order to form a recombinant vector with a polh-AP fusion gene fragment; cloning the polh-AP fusion gene fragment into an expression vector, converting the expression vector to an expression host cell, and culturing the expression host cell to express a fusion protein with an antibacterial peptide AP2; and culturing the expression host cell to induce the expression of the recombinant protein polh-AP, centrifuging induced bacterial strains, collecting, re-suspending, carrying out ultrasonic fragmentation, centrifuging, collecting the above obtained insoluble inclusion body, purifying, and collecting a recombinant protein sample. An expression system has the characteristics of simple expression system culture program, low production cost, efficient expression of the antibacterial peptide apidaecin, and realization of AP2 and polh fusion expression, so compared with the prior art, the method has the advantages of effective reduction of toxicity of the AP2 to Escherichia coli, improvement of the stability of the antibacterial peptide, realization of high level expression of the antibacterial peptide, convenient purification of the active antibacterial peptide, and improvement of the output of the antibacterial peptide apidaecin.

The invention relates to a method for promoting germination through release of pyrus betulaefolia seed dormancy by sodium malonate and belongs to the technical field of plant production. The method for promoting the germination of pyrus betulaefolia seeds comprises the following main steps: 1) collection and cleaning of seeds; 2) seed soaking in clean water and seed disinfection; 3) treatment by a chemical agent and seed cleaning; and 4) sowing. The pyrus betulaefolia seed treated by the sodium malonate is applied, begins to germinate in three days after the sowing and can totally germinate in one week around; and the germination rate is high and can reach more than 85 percent. The method initially finds that the pyrus betulaefolia seed can release the seed dormancy through the treatment of the sodium malonate without low-temperature lamination treatment. The application of the research can effectively shorten the germinating time and cross-breeding cycle of pyrus betulaefolia and simplify the embryo culturing procedure.

The invention discloses a culture medium for culturing antrodia camphorata by vessel culturing and a preparation method thereof. The culture medium is prepared from the following raw materials in parts by weight: 200 to 300 parts of potato leaching powder, 20 to 40 parts of glucose, 0 to 20 parts of soluble soybean meal, 1 to 20 parts of malt extractant, 0.5 to 1.5 parts of magnesium sulfate, 10 to 60 parts of a mixture of a cinnamomum plant, 0.5 to 1.5 parts of potassium dihydrogen phosphate, 0.01 to 0.05 part of vitamin B1, 0 to 0.1 part of chloramphenicol, 15 to 25 parts of agar and 1,000 parts of ultrapure water. According to the culture medium disclosed by the invention, a culturing vessel is used for culturing the antrodia camphorata; the culturing condition has strong controllability; the culturing procedure is simplified; the sundry bacteria contamination can be reduced; the heavy metal pollution is avoided too; the growth increment of antrodia camphorata mycelium or sporocarp is increased as well; and the production cost is lowered.

The invention discloses a method and application for inducing direct somatic embryogenesis. According to the method and application, function genes RID3(AT3G49180) and YUC10(AT1G48910) capable of promoting direct somatic embryogenesis are found. Through ectopic expression of the function genes, inducing can be directly conducted on arabidopsis cotyledons to form the somatic embryo without any callus. The method for inducing direct somatic embryogenesis has the advantages of being simple in culture, short in inducing period, high in inducing frequency and the like, and can be used for study onaspects of differentiation, development and totipotency expression of botanical cells, crop variety improvement, production of artificial seeds, storage of high-quality germplasm resources, mutant screening and the like.

The invention provides a tissue cultivation method of vetiveria zizanioides transplants. The method comprises steps that: sterilizated vetiveria zizanioides explants are placed in induction and proliferation culture media; inducting and proliferation cultivating are carried out concurrently until explants are differentiated and young buds are germinated; times of subcultures are carried out untila required number of seedlings are obtained; the seedlings are transplanted into cultivation media to be cultivated until 3 to 5 roots appear; the seedlings are then transplanted into cultivation bags; routine managements of watering and fertilizing are carried out, and vetiveria zizanioides transplants can be obtained after 40 days of growth. With the method provided by the present invention, usage amounts of drugs can be reduced. Through the concurrent inducting and proliferation cultivating, appearances of variants can be efficiently controlled, tissue cultivating processes can be simplified, propagation periods can be shortened, production cost can be reduced, and merits of vetiveria zizanioides varieties can be kept fast and stably. With the method provided by the present invention, problems in methods with common propagation systems, such as complex parent resources, unstable seedling qualities and slow seedling propagations, can be solved.

A provided dendrobium aphyllum tissue-culture quick propagation method comprises: germination of cracked mature seeds obtained by inbred of dendrobium aphyllum, forming and differentiation of protocorms, rooting culture, culture of complete plants, transplanting and surviving. Compared with conventional propagation methods such as high-position bud cutting or strain segregating, the method provided by the invention employs tissue culture technology for propagation of dendrobium aphyllum, and the tissue culture of dendrobium aphyllum is performed by employing protocorms, so that the propagation speed is fast, a large number of seedlings are obtained and grow well, and thus a large number of dendrobium aphyllum seedlings with same properties are cultured in a short period, the transplanting survival rate is high, the market demand is satisfied, and the method has various advantages such as low cost, short period, high yield and the like.

The invention discloses a medium for cultivating a wild paxillus involutus stock culture. The medium is prepared from, by mass, 15-25g of soluble starch, 2-6g of peptone, 1-5g of KH2PO4, 0.5-4.5g of MgSO4, 1L of water and 2-6 of pH. The medium has the advantages of early germination, rapid growth, good growth, high mycelial biomass, short production cycle and the like, the biological characteristics of the bacterium are mastered, and a foundation is laid for further development of an application value and artificial domestication and cultivation. The successful cultivation of the stock culturealso provides new germplasm resources for the development and utilization of medicinal ingredients and teaching and researching, and has great economic and social benefits.

The invention discloses a tissue culture breeding method for acorus gramineus. The tissue culture breeding method comprises the steps of explant disinfection, induction, enrichment culture, subculture, rooting culture, seedling hardening and transplanting. According to the method, the acorus gramineus is high in reproduction speed, and roots and seedlings are robust; the cost and the production time are greatly saved; the problems that the source of parents of seedlings produced by a conventional breeding system is complicated and the quality of the seedlings is unstable; the tissue culture breeding method is suitable for standard, industrial and large-scale production; high-quality seedlings of the uniform standard are provided for the market; the problem that acorus gramineus seedlings cannot be yearly produced is solved.