30results about How to "Overcome the difficulty of not being able to carry out production on an annual basis" patented technology

The method for raising seedlings of Rhizoma Polygonatum, the steps include: preparation of explants of Rhizoma Rhizoma Diannensis, culture for eliminating browning, callus induction, adventitious bud generation and proliferation culture, dark culture for promoting rhizome growth, rejuvenation culture of test-tube plantlets, rooting culture, hardening of seedlings Cultivation; the beneficial effect is: the Yunnan gold explants are carried out in the substratum A in the transfer culture of every 7 days, and the effect of removing the browning phenomenon occurring in the tissue culture process is remarkable; simultaneously carry out callus induction, cluster bud generation and Proliferation culture improves the reproduction efficiency, the reproduction coefficient can be increased to more than 8.67, and the tissue culture time can be shortened to 45 days; in the dark culture stage, the rhizome of Polygonatum yunnanensis is greatly improved, and the survival rate can reach more than 95%; the invention is divided into stages Four kinds of media are used for transfer culture, which overcomes the problem of easy breeding of fungi and accumulation of viruses when Polygonatum yunnanensis is used for group cultivation of seedlings. The cultivated seedlings have strong adaptability and stress resistance, which reduces the pollution rate of group cultured seedlings and improves To ensure the stability of species.

The invention provides a method for polyploidy induction and efficient artificial seedling culture of polyploidy plants of campanumoea lancifolia, which comprises the following steps: (1) inoculating disinfected and sterilized seeds into a culture medium A for sterile germination; (2) soaking stem tips with leaves of sterile seedlings in a sterilized colchicine solution, then transferring the stem tips into a culture medium B for culture, and then transferring the stem tips into a culture medium C; and (3) completing proliferative rooting of polyploidy plants in the culture medium C in one step. According to the method, the in-vitro rapid propagation process of the campanumoea lancifolia polyploidy plant is optimized and adjusted, proliferation and rooting are synchronously carried out, the tissue culture process is simplified, the whole culture process is carried out in one culture medium at the same time, and the artificial in-vitro propagation efficiency is greatly improved. In addition, a campanumoea lancifolia homologous polyploidy induction system is established; under the condition of delayed polyploidy growth, a direct organ generation mode is adopted for proliferation, meanwhile, a high propagation coefficient is kept, the culture period is greatly shortened, the cost is low, the period is short, and the quality and the survival rate are high.

The invention discloses a fast asexual propagation method for maca hybrid. The method comprises the following steps: inoculating a sterilized maca hybrid explant into a callus inducing medium, generating callus, and the generated callus into a clump seedling generating and breeding medium to cultivate; when clump seedlings are generated and bred to a certain quantity, carrying out subculture multiplication in the breeding medium; when the quantity of the bred seedlings reaches the rooting requirements, transferring healthy main seedlings in the clump seedlings into a rooting medium to cultivate; and when the seedlings are healthy and the roots are thick, carrying out hardening-seedling for 3 days according to a conventional method, and transplanting into a floating plate with disinfection humus and cultivating for 60 days so as to obtain the healthy maca hybrid clonal seedlings. The production technology of the maca hybrid clonal seedlings is optimized and adjusted; after the callus and the clump seedlings are obtained, MS with high NH<4+> and B5 with low NH<4+> are adopted as basic culture mediums for alternative use in enrichment culture; the phenomena that a maca test-tube plantlet is easy to age and yellow are thoroughly solved; the asexual propagation cycle is shortened; the breeding efficiency is improved; and an effective way is provided for breeding of F1 generation of high-quality seedlings of the maca hybrid.

The invention provides a rapid reproduction method of schlumbergera seedlings. The method comprises the following steps: inoculating a selected, disinfected and sterilized schlumbergera explant onto a culture medium for induction and carrying out synchronous induction and proliferation culture until the explant sprouts and differentiates out a bud; sub-culturing until being proliferated to 3-4 folds, and then transferring to a rooting medium for rooting culture until 3-5 fine roots are grown at the base of a plant; conventionally hardening and then transplanting to a mixed substrate of humus soil, red soil and pearlite; and watering and fertilizing conventionally for growing to obtain the schlumbergera seedlings. In the method, key links in the whole production technical flow of the schlumbergera seedlings are optimized and integrated, the culture medium for induction is blended and bud induction and proliferation culture are carried out synchronously, thus simplifying tissue culture procedure and shortening breeding cycle; and only by adopting two types of the culture media, the bud can be directly formed without callus formation, thus reducing probability of variation occurred on an adventitious bud, effectively controlling generation of a variant strain, being capable of fixing and maintaining fine variety of the schlumbergera seedlings rapidly, improving seedling quality and solving the problems of complicated parental source and unstable quality of the seedlings produced by the conventional breeding system.

The invention discloses a new method for artificial rapid propagation of cremastra appendiculata (D.Don) Makino.The method comprises the following steps: (1) obtaining an explant; (2) firstly washingsoil on the surface of pseudobulb in step (1) with tap water, then soaking with a washing powder solution, then rinsing with running water, then treating with an ethanol solution, and rinsing after disinfection; (3) obtaining sterile seedlings; (4) transferring young shoots grown from buds cultured in step (3) to a medium A in step (3) for enrichment culture, cutting off seedlings with callus, andcontinuing to transfer to a medium B for culture; (5) inoculating a medium C with single seedlings with rice grain-sized pseudobulb after subculture in step (4) for culture; (6) exercising the seedlings and transplanting. The method disclosed by the invention has the advantages that the in-vitro rapid propagation of the cremastra appendiculata (D.Don) Makino is optimized and adjusted; the processof artificial rapid propagation is simplified; the propagation efficiency is greatly improved; the cost is low and the time is short; not only is the survival rate of tube seedlings through seedlingexercising improved, but the nursery cycle is also greatly shortened.

The present invention discloses a new method for artificial fast propagation of Shanci mushroom, comprising the following steps: (1) obtaining explants; (2) washing the surface soil of the pseudobulbs in step 1 with tap water, and then soaking them with washing powder solution Rinse with running water, then process with ethanol solution, and rinse after disinfection; (3) obtain sterile seedlings; (4) take the sprouts grown from the bud spots in step 3 and transfer them to the A medium in step 3 Proliferation culture is carried out inside, and the seedlings carrying callus are cut off, and continue to be transferred into B medium to cultivate; (5) the single seedling with rice grain size pseudobulb after subculture in step 4 is inoculated in C medium (6) seedling refining and transplanting. The invention optimizes and adjusts the rapid propagation in vitro of S. chinensis, simplifies the artificial rapid propagation process, greatly improves the propagation efficiency, has low cost and short time, not only improves the survival rate of the test-tube seedlings, but also greatly shortens the seedling raising period. .

The invention discloses a fast asexual propagation method for maca hybrid. The method comprises the following steps: inoculating a sterilized maca hybrid explant into a callus inducing medium, generating callus, and the generated callus into a clump seedling generating and breeding medium to cultivate; when clump seedlings are generated and bred to a certain quantity, carrying out subculture multiplication in the breeding medium; when the quantity of the bred seedlings reaches the rooting requirements, transferring healthy main seedlings in the clump seedlings into a rooting medium to cultivate; and when the seedlings are healthy and the roots are thick, carrying out hardening-seedling for 3 days according to a conventional method, and transplanting into a floating plate with disinfection humus and cultivating for 60 days so as to obtain the healthy maca hybrid clonal seedlings. The production technology of the maca hybrid clonal seedlings is optimized and adjusted; after the callus and the clump seedlings are obtained, MS with high NH<4+> and B5 with low NH<4+> are adopted as basic culture mediums for alternative use in enrichment culture; the phenomena that a maca test-tube plantlet is easy to age and yellow are thoroughly solved; the asexual propagation cycle is shortened; the breeding efficiency is improved; and an effective way is provided for breeding of F1 generation of high-quality seedlings of the maca hybrid.

The invention provides a tissue cultivation method of vetiveria zizanioides transplants. The method comprises steps that: sterilizated vetiveria zizanioides explants are placed in induction and proliferation culture media; inducting and proliferation cultivating are carried out concurrently until explants are differentiated and young buds are germinated; times of subcultures are carried out untila required number of seedlings are obtained; the seedlings are transplanted into cultivation media to be cultivated until 3 to 5 roots appear; the seedlings are then transplanted into cultivation bags; routine managements of watering and fertilizing are carried out, and vetiveria zizanioides transplants can be obtained after 40 days of growth. With the method provided by the present invention, usage amounts of drugs can be reduced. Through the concurrent inducting and proliferation cultivating, appearances of variants can be efficiently controlled, tissue cultivating processes can be simplified, propagation periods can be shortened, production cost can be reduced, and merits of vetiveria zizanioides varieties can be kept fast and stably. With the method provided by the present invention, problems in methods with common propagation systems, such as complex parent resources, unstable seedling qualities and slow seedling propagations, can be solved.

The invention provides a high-efficiency artificial seedling-raising method of high-quality passionflower hybrids, which comprises the steps of: inoculating the sterilized leaf explants in a culture medium, inducing callus, forming clustered buds and proliferating , rooting induction, seedling hardening and transplanting. The present invention optimizes and adjusts the production process of hybrid seedlings of passionflower, synchronously carries out callus growth, cluster bud generation and cluster bud proliferation, simplifies the tissue culture process, and carries out three culture processes in one culture medium at the same time , greatly improving the reproductive efficiency. In addition, the invention solves the phenomena such as plant yellowing and leaf defoliation that are easy to occur in the tissue culture process of Passiflora plants, and has low cost, short cycle, high quality and high survival rate.

A provided dendrobium aphyllum tissue-culture quick propagation method comprises: germination of cracked mature seeds obtained by inbred of dendrobium aphyllum, forming and differentiation of protocorms, rooting culture, culture of complete plants, transplanting and surviving. Compared with conventional propagation methods such as high-position bud cutting or strain segregating, the method provided by the invention employs tissue culture technology for propagation of dendrobium aphyllum, and the tissue culture of dendrobium aphyllum is performed by employing protocorms, so that the propagation speed is fast, a large number of seedlings are obtained and grow well, and thus a large number of dendrobium aphyllum seedlings with same properties are cultured in a short period, the transplanting survival rate is high, the market demand is satisfied, and the method has various advantages such as low cost, short period, high yield and the like.

The invention discloses a tissue culture breeding method for acorus gramineus. The tissue culture breeding method comprises the steps of explant disinfection, induction, enrichment culture, subculture, rooting culture, seedling hardening and transplanting. According to the method, the acorus gramineus is high in reproduction speed, and roots and seedlings are robust; the cost and the production time are greatly saved; the problems that the source of parents of seedlings produced by a conventional breeding system is complicated and the quality of the seedlings is unstable; the tissue culture breeding method is suitable for standard, industrial and large-scale production; high-quality seedlings of the uniform standard are provided for the market; the problem that acorus gramineus seedlings cannot be yearly produced is solved.

The invention provides a rapid reproduction method of schlumbergera seedlings. The method comprises the following steps: inoculating a selected, disinfected and sterilized schlumbergera explant onto a culture medium for induction and carrying out synchronous induction and proliferation culture until the explant sprouts and differentiates out a bud; sub-culturing until being proliferated to 3-4 folds, and then transferring to a rooting medium for rooting culture until 3-5 fine roots are grown at the base of a plant; conventionally hardening and then transplanting to a mixed substrate of humus soil, red soil and pearlite; and watering and fertilizing conventionally for growing to obtain the schlumbergera seedlings. In the method, key links in the whole production technical flow of the schlumbergera seedlings are optimized and integrated, the culture medium for induction is blended and bud induction and proliferation culture are carried out synchronously, thus simplifying tissue culture procedure and shortening breeding cycle; and only by adopting two types of the culture media, the bud can be directly formed without callus formation, thus reducing probability of variation occurred on an adventitious bud, effectively controlling generation of a variant strain, being capable of fixing and maintaining fine variety of the schlumbergera seedlings rapidly, improving seedling quality and solving the problems of complicated parental source and unstable quality of the seedlings produced by the conventional breeding system.

The invention provides a method for polyploid induction of red fruit ginseng and an efficient artificial seedling cultivation method for polyploid plants, comprising the following steps: (1) inoculating the sterilized seeds in medium A for aseptic germination; (2) Get sterile seedlings with leafy stem tips and soak them in sterilized colchicine solution, then transfer them to medium C after cultivating them in medium B; (3) polyploid plants are grown in medium C Proliferation and rooting can be completed in one step. The invention optimizes and adjusts the process of in vitro rapid propagation of polyploid plants of red ginseng, synchronously multiplies and takes root, simplifies the tissue culture process, and carries out the whole culture process in one culture medium at the same time, greatly improving the artificial in vitro propagation efficiency. In addition, the present invention establishes a red fruit ginseng autopolyploid induction system; in the case of delayed polyploid growth, the direct organogenesis method is adopted to proliferate, while maintaining a high reproduction coefficient, greatly shortening the culture period, and reducing the cost. Low cost, short cycle time, high quality and high survival rate.

The invention discloses a Davallia mariesii breeding method, which comprises the following steps: an induction proliferation medium and a rooting medium are provided, the inducting and proliferation cultivating are concurrently carried out, after exercising seedling, and the seedling can be directly transplanted to a seedlings bag for culturing. The invention provides a technical scheme of a key link in a seedling breeding technology process of the Davallia mariesii, the proliferation rate is fast, the reproduction rates can reach 5-7 times during one period with 25-30 days; the rooting culture lasts about 20 days, each plant can breed 5-8 roots, the seedling is robust, the root develops, a tissue culture process is simplified, the cost and production time are greatly reduced, and the Davallia mariesii breeding method is suitable for standardization and industrial-scale production.

The invention provides a pararuellia delavayana reproduction method. The method comprises the steps that a disinfected and sterilized pararuellia delavayana explant is inoculated into different culture media successively for callus induction, bud production and reproduction synchronous culture. According to the pararuellia delavayana reproduction method, the common vitrification phenomenon produced during rapid asexual propagation is avoided, the cost is low, time is short, and the quality and survival rate are high.

The invention discloses an artificial efficient propagation method of swertia nervosa (G.Don)Wall.. According to the method, in the same culture medium, callus induction and basal stem multiple shootgeneration can be conducted simultaneously, basal stem multiple shoot generation and multiplication culture can also be conducted simultaneously, and thus the culture procedure is simplified into three steps; in the whole rapid propagation process, only three kinds of culture mediums are needed to achieve the purposes of callus induction, basal stem multiple shoot generation, and multiplication culture, avoid the phenomena of browning and vitrification and solve the problem of root taking, the inoculation procedures and the culture space are saved, and arrangement of production plans is facilitated; besides, since the inoculation frequency is reduced, the possibility of contamination is lowered; the culture mediums for callus induction and multiplication culture have the function of basalstem multiple shoot generation, so that biological materials better adapt to replacement of the culture mediums, the success rate of tissue culture of the biological materials is increased, and the propagation coefficient is greatly increased.

The present invention innovatively utilizes tidal bioreactors to rapidly propagate lily transplanted seed balls, by cultivating and growing lily seed balls with a diameter of 0.2 to 0.4 cm in conventional solid propagation medium as explants and inserting them into tidal bioreactors. In the bioreactor, the liquid medium formula was replaced during the 50-day cultivation process, and the pollution was controlled by adding drugs, and the seed balls were gradually expanded and rooted. After the seed balls were released from the bottle, the lily seed balls were vernalized, transplanted, Planting methods are managed. The present invention can simplify the tissue culture procedure by gradually carrying out seed ball expansion and rooting culture, greatly reduce the labor cost in conventional solid tissue culture, and can effectively control pollution and the generation of mutant strains, shorten the lily breeding cycle, and save The cost of production can quickly fix and maintain the excellent characters of new lily varieties, and solve the problems of slow breeding speed, mixed provenance and unstable quality of seed balls produced by conventional lily breeding systems.

The invention discloses a method for cultivating maca polyploid plants. The method comprises the steps that disinfected maca seeds are inoculated into an empty B5 culture medium, after the seeds germinate, hypocotyledonary axes of the seeds are taken to be inoculated to a callus induction culture medium with high colchicine to be cultured, obtained calluses are transplanted to a tufted-bud induction and enrichment medium with low colchicine to be cultured, then subculture multiplication is carried out for three to four generations, and maca polyploid multiple shoots are obtained; when the polyploidy multiple shoots grow to 3 cm to 4 cm, single studs are cut off to be transplanted to a rooting medium, and maca polyploid test-tube plantlets can be obtained after culturing of 25 d; after hardening-seedling is carried out on the polyploid test-tube plantlets for 3 d according to the convention, the polyploid test-tube plantlets are transplanted into a floating tray with disinfection humus, and maca polyploid seedlings are obtained after culturing of 60 d. The method has the advantages that screening can be carried out during the callus period, the method is simple and high in inductivity, few chimeras exist, the death rate is low, the polyploid test-tube plantlets can be obtained through meristematic proliferation and obtained polyploid materials, the induction time is greatly shortened, and the induced polyploid plants are good in quality and high in stress resistance.

The invention discloses a method for improving the efficiency of artificial rapid propagation of flaxgrass overcoming burnt tip necrosis, which belongs to the field of biotechnology, and comprises the following steps: inoculating a sterilized and sterilized explant of a segmented stem segment into a culture medium, Induce the germination of axillary buds, the occurrence and proliferation of clustered buds on the base stems, overcome the necrosis of burnt tips, rejuvenate and take root, and transplant hardened seedlings. The invention completely solves the phenomenon of burning tip necrosis common in in vitro rapid propagation of plants of the family Scrophulariaceae, especially of the genus Flax, and has low cost, short cycle, high seedling quality and survival rate. In addition, the production process of aseptic seedlings of C. grandiflorum was optimized and adjusted. While improving burn tip necrosis, axillary bud germination and growth, basal stem cluster bud induction and growth were simultaneously carried out, and the tissue culture process was simplified. Three culture processes At the same time in a culture medium, greatly improving the reproduction efficiency.

The invention discloses a method for artificial high-efficiency propagation of Swertia variegata. In the same medium, callus induction and basal shoot cluster formation can be carried out at the same time, and basal tube cluster bud formation and multiplication culture can be carried out simultaneously. The culture procedure is simplified to three steps; the entire rapid propagation process only needs three kinds of medium to solve the problems of callus, basal shoot clustering bud generation and proliferation, overcoming browning and vitrification, and rooting, saving inoculation procedures and The cultivation space is conducive to the arrangement of production plans; in addition, because the number of inoculations is reduced, the possibility of contamination is reduced, and the medium for callus induction and proliferation culture has the function of basal shoot clustering, which makes the biological material replace the medium It is easier to adapt, thereby improving the success rate of tissue culture of biological materials, and greatly improving the reproduction coefficient.