Patents
Literature
Hiro is an intelligent assistant for R&D personnel, combined with Patent DNA, to facilitate innovative research.
Hiro

30results about How to "Overcome the difficulty of not being able to carry out production on an annual basis" patented technology

Method for cultivating seedlings of polygonatum kingianum

The method for raising seedlings of Rhizoma Polygonatum, the steps include: preparation of explants of Rhizoma Rhizoma Diannensis, culture for eliminating browning, callus induction, adventitious bud generation and proliferation culture, dark culture for promoting rhizome growth, rejuvenation culture of test-tube plantlets, rooting culture, hardening of seedlings Cultivation; the beneficial effect is: the Yunnan gold explants are carried out in the substratum A in the transfer culture of every 7 days, and the effect of removing the browning phenomenon occurring in the tissue culture process is remarkable; simultaneously carry out callus induction, cluster bud generation and Proliferation culture improves the reproduction efficiency, the reproduction coefficient can be increased to more than 8.67, and the tissue culture time can be shortened to 45 days; in the dark culture stage, the rhizome of Polygonatum yunnanensis is greatly improved, and the survival rate can reach more than 95%; the invention is divided into stages Four kinds of media are used for transfer culture, which overcomes the problem of easy breeding of fungi and accumulation of viruses when Polygonatum yunnanensis is used for group cultivation of seedlings. The cultivated seedlings have strong adaptability and stress resistance, which reduces the pollution rate of group cultured seedlings and improves To ensure the stability of species.
Owner:玉溪市祥馨农业技术开发有限公司

Davallia mariesii breeding method

The invention discloses a Davallia mariesii breeding method, which comprises the following steps: an induction proliferation medium and a rooting medium are provided, the inducting and proliferation cultivating are concurrently carried out, after exercising seedling, and the seedling can be directly transplanted to a seedlings bag for culturing. The invention provides a technical scheme of a key link in a seedling breeding technology process of the Davallia mariesii, the proliferation rate is fast, the reproduction rates can reach 5-7 times during one period with 25-30 days; the rooting culture lasts about 20 days, each plant can breed 5-8 roots, the seedling is robust, the root develops, a tissue culture process is simplified, the cost and production time are greatly reduced, and the Davallia mariesii breeding method is suitable for standardization and industrial-scale production.
Owner:FLOWER RES INST OF YUNNAN ACAD OF AGRI SCI

Tissue culture method for strawberry transplant seedlings

Strawberry transplanting seedling tissue culture method, the steps include: A, selection of strawberry explants, B, disinfection and sterilization, C, anti-browning culture, D, callus induction, cluster bud generation and proliferation culture, E, expansion Propagation and cultivation, F, rooting and cultivation, G, seedling hardening and transplanting. The beneficial effect is that the tissue culture procedure of strawberry seedlings is simple, and callus induction, cluster bud formation and proliferation culture can be carried out simultaneously in the same medium; the tissue culture period is short, the reproduction coefficient is high, and the whole rapid propagation process only needs three kinds of culture The problem of browning, callus, cluster bud generation and proliferation, and rooting can be solved based on the basis; adventitious root induction of test-tube seedlings does not add phytohormones, and the survival rate of hardened seedlings is high and grows rapidly; seedlings can maintain the same genotype, Progeny are not prone to browning, and there is little virus accumulation; the tissue culture method of inhibiting browning is low in cost, short in time, and the seedlings produced are of good quality, high survival rate, and stable properties, which can meet the industrial production of high-quality strawberry seedlings need.
Owner:玉溪市祥馨农业技术开发有限公司

Method for polyploidy induction and efficient artificial seedling culture of polyploidy plants of campanumoea lancifolia

ActiveCN113519394AShorten the cultivation cycleEnhanced Physiological and Biochemical ProcessesHorticulture methodsPlant tissue cultureBiotechnologyColchicine
The invention provides a method for polyploidy induction and efficient artificial seedling culture of polyploidy plants of campanumoea lancifolia, which comprises the following steps: (1) inoculating disinfected and sterilized seeds into a culture medium A for sterile germination; (2) soaking stem tips with leaves of sterile seedlings in a sterilized colchicine solution, then transferring the stem tips into a culture medium B for culture, and then transferring the stem tips into a culture medium C; and (3) completing proliferative rooting of polyploidy plants in the culture medium C in one step. According to the method, the in-vitro rapid propagation process of the campanumoea lancifolia polyploidy plant is optimized and adjusted, proliferation and rooting are synchronously carried out, the tissue culture process is simplified, the whole culture process is carried out in one culture medium at the same time, and the artificial in-vitro propagation efficiency is greatly improved. In addition, a campanumoea lancifolia homologous polyploidy induction system is established; under the condition of delayed polyploidy growth, a direct organ generation mode is adopted for proliferation, meanwhile, a high propagation coefficient is kept, the culture period is greatly shortened, the cost is low, the period is short, and the quality and the survival rate are high.
Owner:YUNNAN UNIV OF TRADITIONAL CHINESE MEDICINE

Fast asexual propagation method for maca hybrid

The invention discloses a fast asexual propagation method for maca hybrid. The method comprises the following steps: inoculating a sterilized maca hybrid explant into a callus inducing medium, generating callus, and the generated callus into a clump seedling generating and breeding medium to cultivate; when clump seedlings are generated and bred to a certain quantity, carrying out subculture multiplication in the breeding medium; when the quantity of the bred seedlings reaches the rooting requirements, transferring healthy main seedlings in the clump seedlings into a rooting medium to cultivate; and when the seedlings are healthy and the roots are thick, carrying out hardening-seedling for 3 days according to a conventional method, and transplanting into a floating plate with disinfection humus and cultivating for 60 days so as to obtain the healthy maca hybrid clonal seedlings. The production technology of the maca hybrid clonal seedlings is optimized and adjusted; after the callus and the clump seedlings are obtained, MS with high NH<4+> and B5 with low NH<4+> are adopted as basic culture mediums for alternative use in enrichment culture; the phenomena that a maca test-tube plantlet is easy to age and yellow are thoroughly solved; the asexual propagation cycle is shortened; the breeding efficiency is improved; and an effective way is provided for breeding of F1 generation of high-quality seedlings of the maca hybrid.
Owner:会泽枢康中草药种植有限公司

Tissue culture and rapid propagation method of million stars

The invention provides a tissue culture and rapid propagation method of million stars, which comprises the process steps of: selecting an explant, sterilizing the explant, inducing and culturing an adventitious bud, carrying out enrichment culture on the adventitious bud, subculturing, rooting, carrying out transition transplanting and the like. The million stars is cultured on various corresponding culture media and is directly transplanted until growing into a rooting seedling, wherein the seed evolution rate can reach 90 percent, and the transplanting survival rate is above 95 percent. The method solves the problem on the key technology for establishing in a million stars sterile system, achieves the purposes of high seed entering efficiency, high propagation speed and stable production technology through comprehensive adjustment of hormone, nutrition and culture environment, and realizes massive, standardized and industrialized production of the million stars.
Owner:FLOWER RES INST OF YUNNAN ACAD OF AGRI SCI

Artificial efficient propagation method of aristolochia tuberosa

The invention discloses an artificial efficient propagation method of aristolochia tuberosa. The method comprises the following steps of inoculating a disinfected and sterilized explant with a bud stem segments of the aristolochia tuberosa into a culture medium A for culture, starting propagation culture under the conditions that the illuminance is 1500-2000lx, the illumination time is 10h / d and the temperature is controlled to be 22 + / -1 DEG C, and obtaining a propagation coefficient of 3.57 after 35 days. A material is shorn into the stem segments containing 2-3 joints, the stem segments areinoculated into a culture medium B to be cultured, a clustered cluster system with base adventitious cluster buds and upper axillary buds growing is formed after 45 days, and the propagation coefficient reaches 10.0 or above. When the height of proliferated cluster buds is 3-4cm, single seedlings are inoculated into the culture medium B to be cultured for 45d to obtain rooted seedlings with strong seedlings and thick roots, and then the rooted seedlings are transplanted. The method is low in cost, short in period and high in test-tube plantlet quality and survival rate, provides technical support for protecting wild resources and breeding high-quality plantlets, and can be used for producing excellent plantlets with consistent genotype backgrounds in a short time.
Owner:YUNNAN UNIV OF TRADITIONAL CHINESE MEDICINE

Tide type bioreactor and method for culturing lily bulblets thereof

The invention innovatively utilizes a tide type bioreactor to rapidly propagate lily transplanting small bulblets. The bulblets are cultured in a conventional solid propagation culture medium to grow to obtain the lily small bulblets with the diameter of 0.2-0.4cm to be used as explants; the explants are inoculated into the tide type bioreactor; a liquid culture medium formula is replaced in a culturing process of 50 days; medicines are added to control the pollution and bulblet expansion and rooting are gradually carried out; after the bulblets are discharged out of a bottle, the lily bulblets are managed by conventional vernalization, transplanting and planting methods. According to the invention, bulblet expansion and rooting culture are carried out gradually and a tissue culture procedure can be simplified; the manual cost in conventional solid tissue culture is greatly reduced; the generation of pollution and variant strains can be effectively controlled; the lily breeding period is shortened and the production cost is saved; the good properties of new species of the lilies can be rapidly fixed and kept; the difficulties that the propagating speed of the bulblets produced by a conventional lily propagation system is slow, the provenance is complicated and the quality of the bulblets is not stable are overcome.
Owner:FLOWER RES INST OF YUNNAN ACAD OF AGRI SCI

Rapid reproduction method of schlumbergera seedlings

The invention provides a rapid reproduction method of schlumbergera seedlings. The method comprises the following steps: inoculating a selected, disinfected and sterilized schlumbergera explant onto a culture medium for induction and carrying out synchronous induction and proliferation culture until the explant sprouts and differentiates out a bud; sub-culturing until being proliferated to 3-4 folds, and then transferring to a rooting medium for rooting culture until 3-5 fine roots are grown at the base of a plant; conventionally hardening and then transplanting to a mixed substrate of humus soil, red soil and pearlite; and watering and fertilizing conventionally for growing to obtain the schlumbergera seedlings. In the method, key links in the whole production technical flow of the schlumbergera seedlings are optimized and integrated, the culture medium for induction is blended and bud induction and proliferation culture are carried out synchronously, thus simplifying tissue culture procedure and shortening breeding cycle; and only by adopting two types of the culture media, the bud can be directly formed without callus formation, thus reducing probability of variation occurred on an adventitious bud, effectively controlling generation of a variant strain, being capable of fixing and maintaining fine variety of the schlumbergera seedlings rapidly, improving seedling quality and solving the problems of complicated parental source and unstable quality of the seedlings produced by the conventional breeding system.
Owner:FLOWER RES INST OF YUNNAN ACAD OF AGRI SCI

New method for artificial rapid propagation of cremastra appendiculata (D.Don) Makino

The invention discloses a new method for artificial rapid propagation of cremastra appendiculata (D.Don) Makino.The method comprises the following steps: (1) obtaining an explant; (2) firstly washingsoil on the surface of pseudobulb in step (1) with tap water, then soaking with a washing powder solution, then rinsing with running water, then treating with an ethanol solution, and rinsing after disinfection; (3) obtaining sterile seedlings; (4) transferring young shoots grown from buds cultured in step (3) to a medium A in step (3) for enrichment culture, cutting off seedlings with callus, andcontinuing to transfer to a medium B for culture; (5) inoculating a medium C with single seedlings with rice grain-sized pseudobulb after subculture in step (4) for culture; (6) exercising the seedlings and transplanting. The method disclosed by the invention has the advantages that the in-vitro rapid propagation of the cremastra appendiculata (D.Don) Makino is optimized and adjusted; the processof artificial rapid propagation is simplified; the propagation efficiency is greatly improved; the cost is low and the time is short; not only is the survival rate of tube seedlings through seedlingexercising improved, but the nursery cycle is also greatly shortened.
Owner:YUNNAN UNIV OF TRADITIONAL CHINESE MEDICINE

A New Method of Artificial Rapid Propagation of Arrowhead Mushroom

The present invention discloses a new method for artificial fast propagation of Shanci mushroom, comprising the following steps: (1) obtaining explants; (2) washing the surface soil of the pseudobulbs in step 1 with tap water, and then soaking them with washing powder solution Rinse with running water, then process with ethanol solution, and rinse after disinfection; (3) obtain sterile seedlings; (4) take the sprouts grown from the bud spots in step 3 and transfer them to the A medium in step 3 Proliferation culture is carried out inside, and the seedlings carrying callus are cut off, and continue to be transferred into B medium to cultivate; (5) the single seedling with rice grain size pseudobulb after subculture in step 4 is inoculated in C medium (6) seedling refining and transplanting. The invention optimizes and adjusts the rapid propagation in vitro of S. chinensis, simplifies the artificial rapid propagation process, greatly improves the propagation efficiency, has low cost and short time, not only improves the survival rate of the test-tube seedlings, but also greatly shortens the seedling raising period. .
Owner:YUNNAN UNIV OF TRADITIONAL CHINESE MEDICINE

Asexual Rapid Propagation Method of Maca Hybrid

The invention discloses a fast asexual propagation method for maca hybrid. The method comprises the following steps: inoculating a sterilized maca hybrid explant into a callus inducing medium, generating callus, and the generated callus into a clump seedling generating and breeding medium to cultivate; when clump seedlings are generated and bred to a certain quantity, carrying out subculture multiplication in the breeding medium; when the quantity of the bred seedlings reaches the rooting requirements, transferring healthy main seedlings in the clump seedlings into a rooting medium to cultivate; and when the seedlings are healthy and the roots are thick, carrying out hardening-seedling for 3 days according to a conventional method, and transplanting into a floating plate with disinfection humus and cultivating for 60 days so as to obtain the healthy maca hybrid clonal seedlings. The production technology of the maca hybrid clonal seedlings is optimized and adjusted; after the callus and the clump seedlings are obtained, MS with high NH<4+> and B5 with low NH<4+> are adopted as basic culture mediums for alternative use in enrichment culture; the phenomena that a maca test-tube plantlet is easy to age and yellow are thoroughly solved; the asexual propagation cycle is shortened; the breeding efficiency is improved; and an effective way is provided for breeding of F1 generation of high-quality seedlings of the maca hybrid.
Owner:会泽枢康中草药种植有限公司

Tissue cultivaition method of vetiveria zizanioides transplant

The invention provides a tissue cultivation method of vetiveria zizanioides transplants. The method comprises steps that: sterilizated vetiveria zizanioides explants are placed in induction and proliferation culture media; inducting and proliferation cultivating are carried out concurrently until explants are differentiated and young buds are germinated; times of subcultures are carried out untila required number of seedlings are obtained; the seedlings are transplanted into cultivation media to be cultivated until 3 to 5 roots appear; the seedlings are then transplanted into cultivation bags; routine managements of watering and fertilizing are carried out, and vetiveria zizanioides transplants can be obtained after 40 days of growth. With the method provided by the present invention, usage amounts of drugs can be reduced. Through the concurrent inducting and proliferation cultivating, appearances of variants can be efficiently controlled, tissue cultivating processes can be simplified, propagation periods can be shortened, production cost can be reduced, and merits of vetiveria zizanioides varieties can be kept fast and stably. With the method provided by the present invention, problems in methods with common propagation systems, such as complex parent resources, unstable seedling qualities and slow seedling propagations, can be solved.
Owner:FLOWER RES INST OF YUNNAN ACAD OF AGRI SCI

A kind of high-efficiency artificial seedling raising method of Passiflora high-quality hybrid

The invention provides a high-efficiency artificial seedling-raising method of high-quality passionflower hybrids, which comprises the steps of: inoculating the sterilized leaf explants in a culture medium, inducing callus, forming clustered buds and proliferating , rooting induction, seedling hardening and transplanting. The present invention optimizes and adjusts the production process of hybrid seedlings of passionflower, synchronously carries out callus growth, cluster bud generation and cluster bud proliferation, simplifies the tissue culture process, and carries out three culture processes in one culture medium at the same time , greatly improving the reproductive efficiency. In addition, the invention solves the phenomena such as plant yellowing and leaf defoliation that are easy to occur in the tissue culture process of Passiflora plants, and has low cost, short cycle, high quality and high survival rate.
Owner:YUNNAN UNIV OF TRADITIONAL CHINESE MEDICINE

A kind of tissue culture breeding method of P.

The invention discloses a tissue culture breeding method for acorus gramineus. The tissue culture breeding method comprises the steps of explant disinfection, induction, enrichment culture, subculture, rooting culture, seedling hardening and transplanting. According to the method, the acorus gramineus is high in reproduction speed, and roots and seedlings are robust; the cost and the production time are greatly saved; the problems that the source of parents of seedlings produced by a conventional breeding system is complicated and the quality of the seedlings is unstable; the tissue culture breeding method is suitable for standard, industrial and large-scale production; high-quality seedlings of the uniform standard are provided for the market; the problem that acorus gramineus seedlings cannot be yearly produced is solved.
Owner:FLOWER RES INST OF YUNNAN ACAD OF AGRI SCI

Artificial efficient propagation method for primula denticulata

The invention provides an artificial efficient propagation method for primula denticulate. The method comprises the following steps: a culture medium A is inoculated with leaf explants of primula denticulate after sterilization treatment for culture, and callus induction and cluster bud generation culture are carried out under the conditions that the illuminance is 1800-2300 lx, illumination lastsfor 8 h / d and temperature is controlled at 20 + / - 1 DEG C; after 30 d, induced cluster buds are transferred in a fresh culture medium A, and after 30 d, the propagation coefficient of adventitious buds is up to about 20.00. When the proliferated cluster buds are 5-8 cm high, a culture medium B is inoculated with single seedlings for culture, strong rooting seedlings with thick roots are obtainedaft er 25-30 d, and then, the rooting seedlings are transplanted. The method is low in cost, short in cycle and high in quality and survival rate of test-tube plantlets, provides technical support forprotecting wild resources and high-quality seedling propagation, and can produce excellent seedlings with consistent genotype background in a short time.
Owner:云南华农农业有限公司

Rapid reproduction method of schlumbergera seedlings

The invention provides a rapid reproduction method of schlumbergera seedlings. The method comprises the following steps: inoculating a selected, disinfected and sterilized schlumbergera explant onto a culture medium for induction and carrying out synchronous induction and proliferation culture until the explant sprouts and differentiates out a bud; sub-culturing until being proliferated to 3-4 folds, and then transferring to a rooting medium for rooting culture until 3-5 fine roots are grown at the base of a plant; conventionally hardening and then transplanting to a mixed substrate of humus soil, red soil and pearlite; and watering and fertilizing conventionally for growing to obtain the schlumbergera seedlings. In the method, key links in the whole production technical flow of the schlumbergera seedlings are optimized and integrated, the culture medium for induction is blended and bud induction and proliferation culture are carried out synchronously, thus simplifying tissue culture procedure and shortening breeding cycle; and only by adopting two types of the culture media, the bud can be directly formed without callus formation, thus reducing probability of variation occurred on an adventitious bud, effectively controlling generation of a variant strain, being capable of fixing and maintaining fine variety of the schlumbergera seedlings rapidly, improving seedling quality and solving the problems of complicated parental source and unstable quality of the seedlings produced by the conventional breeding system.
Owner:FLOWER RES INST OF YUNNAN ACAD OF AGRI SCI

A method for polyploid induction of red fruit ginseng and efficient artificial seedling cultivation of polyploid plants

ActiveCN113519394BShorten the cultivation cycleEnhanced Physiological and Biochemical ProcessesPlant tissue cultureHorticulture methodsOrganogenesisColchicine
The invention provides a method for polyploid induction of red fruit ginseng and an efficient artificial seedling cultivation method for polyploid plants, comprising the following steps: (1) inoculating the sterilized seeds in medium A for aseptic germination; (2) Get sterile seedlings with leafy stem tips and soak them in sterilized colchicine solution, then transfer them to medium C after cultivating them in medium B; (3) polyploid plants are grown in medium C Proliferation and rooting can be completed in one step. The invention optimizes and adjusts the process of in vitro rapid propagation of polyploid plants of red ginseng, synchronously multiplies and takes root, simplifies the tissue culture process, and carries out the whole culture process in one culture medium at the same time, greatly improving the artificial in vitro propagation efficiency. In addition, the present invention establishes a red fruit ginseng autopolyploid induction system; in the case of delayed polyploid growth, the direct organogenesis method is adopted to proliferate, while maintaining a high reproduction coefficient, greatly shortening the culture period, and reducing the cost. Low cost, short cycle time, high quality and high survival rate.
Owner:YUNNAN UNIV OF TRADITIONAL CHINESE MEDICINE

Davallia mariesii breeding method

The invention discloses a Davallia mariesii breeding method, which comprises the following steps: an induction proliferation medium and a rooting medium are provided, the inducting and proliferation cultivating are concurrently carried out, after exercising seedling, and the seedling can be directly transplanted to a seedlings bag for culturing. The invention provides a technical scheme of a key link in a seedling breeding technology process of the Davallia mariesii, the proliferation rate is fast, the reproduction rates can reach 5-7 times during one period with 25-30 days; the rooting culture lasts about 20 days, each plant can breed 5-8 roots, the seedling is robust, the root develops, a tissue culture process is simplified, the cost and production time are greatly reduced, and the Davallia mariesii breeding method is suitable for standardization and industrial-scale production.
Owner:FLOWER RES INST OF YUNNAN ACAD OF AGRI SCI

Artificial efficient propagation method for primula denticulata

The invention discloses an artificial efficient propagation method for primula denticulata. The method comprises the following steps of inoculating disinfected and sterilized primula denticulata leafexplants into a culture medium A for culture, carrying out callus induction and clump bud occurrence culture under the conditions that the illuminance is 1800-2300lx, the illumination time is 8h / d andthe temperature is controlled to be 20+ / -1 DEG C, transferring induced clump buds into a fresh culture medium A for culturing after 30 days, and enabling the adventitious bud propagation coefficientto reach about 20.00 after 30 days; and when the height of the proliferated clump buds is 5-8 cm, inoculating single seedlings into a culture medium B, culturing for 25-30 days to obtain rooting seedlings with strong seedlings and thick roots, and then transplanting the rooting seedlings. The method is low in cost, short in period and high in test-tube plantlet quality and survival rate, providestechnical support for protecting wild resources and breeding high-quality plantlets, and can be used for producing excellent plantlets with consistent genotype backgrounds in a short time.
Owner:YUNNAN UNIV OF TRADITIONAL CHINESE MEDICINE

A method for artificial rapid propagation of orchid orchids by using embryogenic callus

The invention discloses a method for artificial rapid propagation of orchid orchids by using embryogenic callus, which comprises the following steps: cutting open the sterilized and sterilized capsules, inoculating the seeds in the germination medium, and waiting for healing After the wound tissue appeared, embryo callus induction and proliferation, protocorm differentiation, growth and proliferation, rooting culture, seedling hardening and transplanting were carried out. The core of the present invention is that the embryogenic callus is produced after the seed coat of Orchid orchid is broken, and then differentiated into protocorms; on this basis, the culture medium is optimized and adjusted, and the embryogenic callus is proliferated, the protocorm is differentiated, Germination growth and proliferation are carried out simultaneously, which simplifies the tissue culture process, and the three culture processes are carried out in one medium at the same time, which greatly improves the reproduction efficiency. In addition, the invention has low cost, short period, high seedling quality and survival rate.
Owner:YUNNAN UNIV OF TRADITIONAL CHINESE MEDICINE

Artificial efficient propagation method of swertia nervosa (G.Don)Wall.

The invention discloses an artificial efficient propagation method of swertia nervosa (G.Don)Wall.. According to the method, in the same culture medium, callus induction and basal stem multiple shootgeneration can be conducted simultaneously, basal stem multiple shoot generation and multiplication culture can also be conducted simultaneously, and thus the culture procedure is simplified into three steps; in the whole rapid propagation process, only three kinds of culture mediums are needed to achieve the purposes of callus induction, basal stem multiple shoot generation, and multiplication culture, avoid the phenomena of browning and vitrification and solve the problem of root taking, the inoculation procedures and the culture space are saved, and arrangement of production plans is facilitated; besides, since the inoculation frequency is reduced, the possibility of contamination is lowered; the culture mediums for callus induction and multiplication culture have the function of basalstem multiple shoot generation, so that biological materials better adapt to replacement of the culture mediums, the success rate of tissue culture of the biological materials is increased, and the propagation coefficient is greatly increased.
Owner:JISHOU UNIVERSITY

A kind of cultivation method of Maca polyploid plant

The invention discloses a method for cultivating maca polyploid plants. The method comprises the steps that disinfected maca seeds are inoculated into an empty B5 culture medium, after the seeds germinate, hypocotyledonary axes of the seeds are taken to be inoculated to a callus induction culture medium with high colchicine to be cultured, obtained calluses are transplanted to a tufted-bud induction and enrichment medium with low colchicine to be cultured, then subculture multiplication is carried out for three to four generations, and maca polyploid multiple shoots are obtained; when the polyploidy multiple shoots grow to 3 cm to 4 cm, single studs are cut off to be transplanted to a rooting medium, and maca polyploid test-tube plantlets can be obtained after culturing of 25 d; after hardening-seedling is carried out on the polyploid test-tube plantlets for 3 d according to the convention, the polyploid test-tube plantlets are transplanted into a floating tray with disinfection humus, and maca polyploid seedlings are obtained after culturing of 60 d. The method has the advantages that screening can be carried out during the callus period, the method is simple and high in inductivity, few chimeras exist, the death rate is low, the polyploid test-tube plantlets can be obtained through meristematic proliferation and obtained polyploid materials, the induction time is greatly shortened, and the induced polyploid plants are good in quality and high in stress resistance.
Owner:会泽枢康中草药种植有限公司

The method of overcoming burning tip necrosis and improving the efficiency of artificial rapid propagation of Flaxa grandiflorum

The invention discloses a method for improving the efficiency of artificial rapid propagation of flaxgrass overcoming burnt tip necrosis, which belongs to the field of biotechnology, and comprises the following steps: inoculating a sterilized and sterilized explant of a segmented stem segment into a culture medium, Induce the germination of axillary buds, the occurrence and proliferation of clustered buds on the base stems, overcome the necrosis of burnt tips, rejuvenate and take root, and transplant hardened seedlings. The invention completely solves the phenomenon of burning tip necrosis common in in vitro rapid propagation of plants of the family Scrophulariaceae, especially of the genus Flax, and has low cost, short cycle, high seedling quality and survival rate. In addition, the production process of aseptic seedlings of C. grandiflorum was optimized and adjusted. While improving burn tip necrosis, axillary bud germination and growth, basal stem cluster bud induction and growth were simultaneously carried out, and the tissue culture process was simplified. Three culture processes At the same time in a culture medium, greatly improving the reproduction efficiency.
Owner:YUNNAN UNIV OF TRADITIONAL CHINESE MEDICINE

Novel method for callus induction and efficient plant regeneration of pineapple variant

The invention discloses a novel method for callus induction and efficient plant regeneration of a pineapple variant. The novel method comprises the following steps: (1) obtaining an absorptive bud explant; (2) flushing and disinfecting absorptive buds in step (1), and inoculating the absorptive buds into a culture medium A to obtain calluses; (3) transferring the calluses generated from a root of a leaf base in step (2) into a culture medium B to perform callus proliferation and adventitious bud generation culture; (4) inoculating single seedlings with thick and strong basal stems in adventitious clumpy buds in step (3) into a culture medium C for rooting culture; and (5) seedling hardening and transplanting. In-vitro rapid propagation of the pineapple variant is optimized and adjusted, callus induction and proliferation and adventitious bud generation and proliferation are synchronously carried out, the tissue culture process is simplified, four culture processes are simultaneously carried out in one culture medium, the propagation efficiency is greatly improved, the cost is low, the time is short, the problem of low survival rate of seedling hardening and domestication of the pineapple test-tube plantlet is solved, and the seedling raising period is greatly shortened.
Owner:YUNNAN UNIV OF TRADITIONAL CHINESE MEDICINE

Efficient artificial seedling raising method for high-quality hybrids of passiflora coerulea L.

ActiveCN110521598AMaintain multiplication factorInhibit the occurrence of vitrified seedlingsHorticulture methodsPlant tissue cultureLeaf fallShort cycle
The invention provides an efficient artificial seedling raising method for high-quality hybrids of passiflora coerulea L.. The method comprises the steps that disinfected and sterilized leaf explantsare inoculated into a culture medium, and callus induction, cluster bud generation and proliferation, rooting induction and seedling hardening and transplanting are conducted. By means of the method,a production technology for hybrid seedlings of passiflora coerulea L. is optimized and adjusted, callus growth, cluster bud generation and cluster bud proliferation are conducted synchronously, the tissue culture process is simplified, the three culture processes are performed simultaneously in one culture medium, and the reproduction efficiency is greatly improved. In addition, the method solvesthe problems of plant yellowing yellowing, leaf falling and the like which easily occur in the tissue culture process of passiflora plants, and has the advantages of low cost, short cycle and high quality and survival rate.
Owner:YUNNAN UNIV OF TRADITIONAL CHINESE MEDICINE
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products